AGGF1

General Information

Gene Name : AGGF1
Gene ID : ENSG00000164252.12
Essential Gene : No

Functions

Novel RBP

Localization(RBPImage: AGGF1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Cytoplasm
Compartment/Organelle
Mitochondria

Domains (AlphaFold: AGGF1)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A303-634A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	AGGF1	ENSG00000164252.12	14.90	12.80	13.85	ENCSR000AEF
HepG2	AGGF1	ENSG00000164252.12	4.64	9.90	7.27	ENCSR985KAT
K562	AGGF1	ENSG00000164252.12	7.74	8.69	8.21	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 537	ENCSR725ARB	AGGF1	K562	Released	ENCAB191KLA	ENCSR910LAN
eCLIP	eCLIP: 543	ENCSR543TPH	AGGF1	HepG2	Released	ENCAB191KLA	ENCSR182AEG
knockdown followed by RNA-seq	AGGF1-BGKLV32-K562	ENCSR812TLY	AGGF1	K562	Released		ENCSR143COQ	shRNA	KD% = 78.7,81.8
knockdown followed by RNA-seq	AGGF1-BGHcLV05-HepG2	ENCSR666QJU	AGGF1	HepG2	Released		ENCSR805TIZ	CRISPR	KD% = 94.5,96.2

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
AGGF1	537	K562	ENCSR725ARB	ENCSR910LAN	ENCAB191KLA	A303-634A	1	Released	ENCODE3
AGGF1	543	HepG2	ENCSR543TPH	ENCSR182AEG	ENCAB191KLA	A303-634A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
AGGF1	ENCAB191KLA	A303-634A	1		Released, ENCODE3	Released, ENCODE3	BGHLV31-7, Released, ENCODE3	BGKLV32-5, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
AGGF1	K562	shRNA	ENCSR812TLY	ENCSR143COQ	AGGF1_BGKLV32-5,AGGF1_BGKLV32-6	NT_BGKLV32-1,NT_BGKLV32-2	78.7,81.8	Released	ENCODE3
AGGF1	HepG2	shRNA			BGHLV31-7,BGHLV31-8	BGHLV31-1,BGHLV31-2		NotSatisfied	ENCODE3
AGGF1	HepG2	CRISPR	ENCSR666QJU	ENCSR805TIZ	AGGF1-BGHcLV05-5D,AGGF1-BGHcLV05-6D	NT-BGHcLV05-1D,NT-BGHcLV05-2D	94.5,96.2	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

AGGF1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Cytoplasm

Compartment/Organelle

Mitochondria

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
AGGF1	Product_ID: A303-634A Lot_ID: 1 Source: Bethyl Labs Target Name: AGGF1-human

RNA Targets

AGGF1 protein binds to 8,508 RNA regions.

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Proteins bound to RNA

AGGF1 RNA is bound by 4 proteins.

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AGGF1 protein has 8,508 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
AGGF1	chr11	2154393	2154397	-	3.77989	400.00000	HepG2	IGF2, INS-IGF2	3'-UTR,CDS,intron-Distal
AGGF1	chr21	46904264	46904273	+	7.01534	400.00000	K562	COL18A1	intron-Distal
AGGF1	chr18	75697040	75697048	-	6.39795	400.00000	K562	RP11-671C19.2, LINC01029	intron-Distal
AGGF1	chr11	2152958	2152961	-	5.74098	400.00000	HepG2	IGF2	3'-UTR,intron-Distal
AGGF1	chr22	49851989	49852048	-	6.46171	400.00000	K562	C22orf34	intron-Distal
AGGF1	chr11	2152788	2152793	-	6.60779	400.00000	HepG2	IGF2	3'-UTR,intron-Distal
AGGF1	chr17	75466160	75466238	+	6.46877	400.00000	HepG2	SEPT9, RP11-75C10.7	intron-Distal
AGGF1	chr11	2152887	2152889	-	6.11271	400.00000	HepG2	IGF2	3'-UTR,intron-Distal
AGGF1	chr21	47614065	47614144	-	6.48035	400.00000	K562	LSS	intron-Distal
AGGF1	chr22	49870772	49870887	-	9.49468	400.00000	K562	C22orf34	intron-Distal

Showing 1 to 10 of 100 entries

4 proteins are bound to AGGF1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr5	76344002	76344100	+	CDS,intron-Proximal	3.43746	6.60490	HepG2	BCLAF1
chr5	76344759	76344811	+	CDS,intron-Proximal	3.39721	5.45876	HepG2	BCLAF1
chr5	76357543	76357633	+	CDS,intron-Proximal	3.11413	5.41373	HepG2	PPIG
chr5	76360933	76361000	+	3'-UTR	5.94783	5.13070	K562	PUM2
chr5	76336615	76336741	+	intron-Distal	4.12094	5.07626	HepG2	PTBP1
chr5	76332380	76332451	+	3'-UTR,CDS,intron-Proximal	3.36707	4.74886	HepG2	BCLAF1
chr5	76349808	76349890	+	CDS	3.47413	4.74886	HepG2	BCLAF1
chr5	76331483	76331570	+	3'-UTR,CDS,intron-Proximal	3.33762	4.56058	HepG2	BCLAF1
chr5	76342177	76342235	+	CDS	3.53034	3.68471	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 9 of 9 entries

No autoregularoty interactions between protein AGGF1 and its RNA.

Diffrential Expression

8,361 differentially expressed genes after Knockdown/Knockout AGGF1 protein.

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81 RBPs can affect the levels of AGGF1.

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8,361 differentially expressed genes after Knockdown/Knockout AGGF1 protein :

Only display 100 entries as example. Please click "View All" button to see all 8,361 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
ETV1	ENSG00000006468.9	292.04600	808.91210	-1.46980	32.09880	30.25360	K562	AGGF1
FKBP4	ENSG00000004478.5	4633.42340	2748.36240	0.75340	30.02760	28.24610	K562	AGGF1
ARF5	ENSG00000004059.6	4280.21350	2562.14090	0.74020	27.69240	26.00200	K562	AGGF1
PHTF2	ENSG00000006576.12	3465.71370	2148.90730	0.68940	22.71930	21.20470	K562	AGGF1
CD99	ENSG00000002586.13	1115.98450	612.45430	0.86550	18.45110	17.10640	K562	AGGF1
RPAP3	ENSG00000005175.5	1173.33180	1918.91960	-0.70970	17.56240	16.25540	K562	AGGF1
POLR2J	ENSG00000005075.11	3105.92960	2061.26620	0.59140	16.23900	14.98370	K562	AGGF1
WDR54	ENSG00000005448.12	420.21210	182.05800	1.20660	16.04510	14.79390	K562	AGGF1
KRIT1	ENSG00000001631.10	1223.79580	1918.73560	-0.64880	15.33950	14.11640	K562	AGGF1
SLC25A5	ENSG00000005022.5	12834.28960	9313.45650	0.46250	15.24050	14.02000	K562	AGGF1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

81 RBPs can affect the gene expression of AGGF1 :

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Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
AGGF1	ENSG00000164252.8	86.68370	959.84840	-3.46900	126.01930	121.62380	K562	AGGF1
AGGF1	ENSG00000164252.8	237.51630	990.59420	-2.06030	46.21230	43.91910	K562	PUS1
AGGF1	ENSG00000164252.8	374.05740	998.75080	-1.41690	27.72100	25.25740	K562	AKAP8L
AGGF1	ENSG00000164252.8	410.33440	965.09570	-1.23390	26.11440	24.21520	K562	RBM15
AGGF1	ENSG00000164252.8	429.36920	904.51740	-1.07490	22.66980	21.20190	HepG2	UTP3
AGGF1	ENSG00000164252.8	617.28270	974.20530	-0.65830	12.82320	11.74500	HepG2	PUM1
AGGF1	ENSG00000164252.8	1105.75400	683.47600	0.69400	9.74040	8.27550	K562	POLR2G
AGGF1	ENSG00000164252.8	599.68090	1059.97680	-0.82180	9.83790	8.02170	K562	KIF1C
AGGF1	ENSG00000164252.8	472.29530	708.03660	-0.58410	7.63090	6.69140	HepG2	PUS1
AGGF1	ENSG00000164252.8	628.41650	903.10540	-0.52320	7.12860	6.33130	HepG2	PTBP1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 87 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

2,984 alternative splicing events after Knockdown/Knockout AGGF1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	HIRA	ENSG00000100084.10	chr22	-	19395796	19395930	19394706	19394797	19396005	19396116	0.21300	0.00002	included	K562	AGGF1
SE	FOPNL	ENSG00000133393.8	chr16	-	15976805	15976905	15973660	15973745	15977864	15978062	0.11300	0.00000	included	K562	AGGF1
SE	SPRED2	ENSG00000198369.5	chr2	-	65571569	65571728	65561762	65561907	65571852	65571920	0.25400	0.00004	included	K562	AGGF1
SE	WAC	ENSG00000095787.17	chr10	+	28900183	28900410	28899627	28899750	28900702	28900851	0.07100	0.00000	included	K562	AGGF1
SE	WAC	ENSG00000095787.17	chr10	+	28884661	28884970	28879648	28879761	28897114	28897360	0.05600	0.00018	included	K562	AGGF1
SE	PKP4	ENSG00000144283.17	chr2	+	159533250	159533379	159530394	159530512	159535092	159535166	0.19100	0.00001	included	K562	AGGF1
SE	KIF23	ENSG00000137807.9	chr15	+	69733106	69733418	69732646	69732826	69737128	69737380	0.35000	0.00000	included	K562	AGGF1
SE	ALDOA	ENSG00000149925.12	chr16	+	30077196	30077248	30075744	30075826	30078554	30078679	0.15900	0.00000	included	K562	AGGF1
SE	CBX5	ENSG00000094916.9	chr12	-	54668023	54668125	54651297	54651476	54673791	54673886	0.05200	0.00016	included	K562	AGGF1
SE	PSMD6	ENSG00000163636.6	chr3	-	64008318	64008477	64007993	64008199	64008950	64009148	0.12900	0.00002	included	K562	AGGF1

Showing 1 to 10 of 100 entries

2 RBPs can affect the splicing of AGGF1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	AGGF1	ENSG00000164252.8	chr5	+	76356294	76356345	76355440	76355568	76357527	76357627	0.05500	0.00077	0.06707	included	HepG2	SRSF7
A3SS	AGGF1	ENSG00000164252.8	chr5	+	76330226	76330345	76330242	76330345	76326280	76326801	0.22800	0.00354	0.02132	longer	K562	AQR
A3SS	AGGF1	ENSG00000164252.8	chr5	+	76344739	76344812	76344760	76344812	76343985	76344097	0.06100	0.01258	0.06433	longer	K562	AQR

Showing 1 to 3 of 3 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

AGGF1

General Information

Functions

Localization(RBPImage: AGGF1)

Domains (AlphaFold: AGGF1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

AGGF1 protein has 8,508 RNA targets:

4 proteins are bound to AGGF1 RNA:

No autoregularoty interactions between protein AGGF1 and its RNA.

Diffrential Expression

8,361 differentially expressed genes after Knockdown/Knockout AGGF1 protein :

81 RBPs can affect the gene expression of AGGF1 :

2,984 alternative splicing events after Knockdown/Knockout AGGF1 protein :

2 RBPs can affect the splicing of AGGF1 :

No autoregularoty alternative splicing events between protein AGGF1 and its RNA.