AGGF1      shRNA in HepG2      Batch: BGHLV31

Experiment Information (Status: NotSatisfied)
BGHLV31-7BGHLV31-8
idx755756
TRCN#_or_BGC#TRCN0000129956TRCN0000129956
shRNA_or_gRNA_sequenceGCTCATCATTAGCTGAAAGTTGCTCATCATTAGCTGAAAGTT
PAM
NameAGGF1AGGF1
Sample_IDBGHLV31-7BGHLV31-8
transduction_Date9/24/20159/24/2015
days
RBP_nameAGGF1AGGF1
qPCR_result6.320.0
Ave_qPCR13.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc-1204330
WB_result
Ave_WB
WB_DONE_date
MW
IP1IP1IP
antibody_Cat#A303-634AA303-634A
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM14.50
Rep2_TPM13.90
Action
Library_start_date
repeat_library
Note
ID28442845




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
AGGF1Product_ID: A303-634A
Lot_ID: 1
Source: Bethyl Labs
Target Name: AGGF1-human		 HepG2_Bethyl_A303-634A_1_AGGF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AGGF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AGGF1 antibody (lanes under 'AGGF1').
 AGGF1-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against AGGF1 in HepG2 whole cell lysate using AGGF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AGGF1. AGGF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 K562_Bethyl_A303-634A_1_AGGF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AGGF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AGGF1 antibody (lanes under 'AGGF1').
 AGGF1-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against AGGF1 in K562 whole cell lysate using AGGF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AGGF1.AGGF1 protein appears as the green band, Tubulin serves as a control and appears in red.




Experiment Status
BGHLV31-7BGHLV31-8
Sample_IDBGHLV31-7BGHLV31-8
Sample Name
Sample Name
RBPAGGF1AGGF1
Cell_LineHepG2HepG2
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-06-262019-06-26
ProjectENCODE3ENCODE3
Note
ID21472148




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database