PPP1R8

General Information

Gene Name : PPP1R8
Gene ID : ENSG00000117751.17
Essential Gene : Yes

Functions

Splicing regulation

Localization(RBPImage: PPP1R8)

Nuclei
Unknown Nuclear Foci
Cytoplasm
Cytosolic

Domains (AlphaFold: PPP1R8)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

MBLI: RN068PW

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	PPP1R8	ENSG00000117751.17	38.96	47.29	43.12	ENCSR000AEF
HepG2	PPP1R8	ENSG00000117751.17	70.59	53.76	62.17	ENCSR985KAT
K562	PPP1R8	ENSG00000117751.17	95.27	72.23	83.75	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	PPP1R8-BGKLV28-K562	ENCSR844QNT	PPP1R8	K562	Released		ENCSR164MUK	shRNA	KD% = 43.2,57.4
knockdown followed by RNA-seq	PPP1R8-BGHcLV12-HepG2	ENCSR592AQT	PPP1R8	HepG2	Released		ENCSR827PII	CRISPR	KD% = 74.3,67.6

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
PPP1R8	ENCAB563YNZ	RN068PW	1			NotSatisfied, ENCODE4	BGHcLV12-63, Released, ENCODE4	BGKLV28-41, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
PPP1R8	K562	shRNA			BGKLV13-17,BGKLV13-18	BGKLV13-1,BGKLV13-2		NotSatisfied	ENCODE3
PPP1R8	K562	shRNA	ENCSR844QNT	ENCSR164MUK	PPP1R8_BGKLV28-41,PPP1R8_BGKLV28-42	non-target_BGKLV28-1,non-target_BGKLV28-2	43.2,57.4	Released	ENCODE3
PPP1R8	HepG2	shRNA			BGHLV23-29,BGHLV23-30	BGHLV23-1,BGHLV23-2	0.0,0.0	NotSatisfied	ENCODE3
PPP1R8	HepG2	shRNA			BGHLV31-49,BGHLV31-50	BGHLV31-1,BGHLV31-2		NotSatisfied	ENCODE3
PPP1R8	HepG2	CRISPR	ENCSR592AQT	ENCSR827PII	PPP1R8-BGHcLV12-63,PPP1R8-BGHcLV12-64	NT-BGHcLV12-1,NT-BGHcLV12-2	74.3,67.6	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

PPP1R8: Click to RBP Image Database

Nuclei

Unknown Nuclear Foci

Cytoplasm

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
PPP1R8	Product_ID: RN068PW Lot_ID: 1 Source: MBLI Target Name: PPP1R8-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for PPP1R8 is not avaliable on ENCORE database.

Proteins bound to RNA

PPP1R8 RNA is bound by 35 proteins.

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eCLIP assay for PPP1R8 is not avaliable on ENCORE database.

35 proteins are bound to PPP1R8 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr1	28177914	28178020	+	3'-UTR,intron-Distal,intron-Proximal	6.54726	400.00000	K562	PUM2
chr1	28160980	28161000	+	intron-Distal	6.32927	400.00000	HepG2	DKC1
chr1	28161044	28161055	+	intron-Distal	6.59737	400.00000	HepG2	DKC1
chr1	28161005	28161008	+	intron-Distal	8.00243	400.00000	HepG2	DKC1
chr1	28160916	28160931	+	intron-Distal	8.24463	400.00000	HepG2	DKC1
chr1	28160907	28160916	+	intron-Distal	8.12388	400.00000	HepG2	DKC1
chr1	28161022	28161044	+	intron-Distal	6.88780	400.00000	HepG2	DKC1
chr1	28160931	28160954	+	intron-Distal	8.38299	400.00000	HepG2	DKC1
chr1	28161019	28161067	+	intron-Distal	3.00666	400.00000	K562	PTBP1
chr1	28161013	28161022	+	intron-Distal	7.72772	400.00000	HepG2	DKC1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 66 entries

eCLIP assay for PPP1R8 is not avaliable on ENCORE database.

Diffrential Expression

942 differentially expressed genes after Knockdown/Knockout PPP1R8 protein.

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79 RBPs can affect the levels of PPP1R8.

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942 differentially expressed genes after Knockdown/Knockout PPP1R8 protein :

Only display 100 entries as example. Please click "View All" button to see all 942 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
NGFR	ENSG00000064300.4	127.53600	24.55240	2.37690	8.94350	5.85860	K562	PPP1R8
RHBDF1	ENSG00000007384.11	871.28430	309.48200	1.49320	8.79030	5.76610	K562	PPP1R8
ASPM	ENSG00000066279.12	1901.28250	4210.46390	-1.14700	7.63070	4.77630	K562	PPP1R8
ZNF37A	ENSG00000075407.13	436.24600	1037.88680	-1.25040	7.53290	4.73200	K562	PPP1R8
LRCH4	ENSG00000077454.11	3658.27620	1721.58410	1.08730	6.60360	4.01010	K562	PPP1R8
RPS5	ENSG00000083845.4	14018.83010	6708.94890	1.06310	6.48350	3.93640	K562	PPP1R8
PLEKHH3	ENSG00000068137.10	2006.90200	939.12120	1.09550	6.41220	3.89550	K562	PPP1R8
GOPC	ENSG00000047932.9	893.81350	1859.64870	-1.05700	6.05150	3.59490	K562	PPP1R8
PHPT1	ENSG00000054148.13	1483.36600	621.49670	1.25500	6.01640	3.57980	K562	PPP1R8
FTL	ENSG00000087086.9	93986.83620	46488.97200	1.01550	5.96840	3.55090	K562	PPP1R8
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

79 RBPs can affect the gene expression of PPP1R8 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PPP1R8	ENSG00000117751.13	2054.22850	1260.55250	0.70440	16.05610	14.70580	K562	FXR1
PPP1R8	ENSG00000117751.13	557.30080	899.69150	-0.69100	11.64260	10.60270	HepG2	TRIM56
PPP1R8	ENSG00000117751.13	631.93900	1004.70560	-0.66890	11.74560	10.48670	HepG2	UPF1
PPP1R8	ENSG00000117751.13	484.61030	901.36040	-0.89530	11.00830	9.68960	HepG2	SF3B1
PPP1R8	ENSG00000117751.13	761.06190	1104.98400	-0.53790	9.77920	8.90910	HepG2	ZNF622
PPP1R8	ENSG00000117751.13	1821.79560	1297.85750	0.48910	8.94060	8.19840	HepG2	SRSF1
PPP1R8	ENSG00000117751.13	855.10080	1293.46030	-0.59710	8.64740	7.63720	HepG2	QKI
PPP1R8	ENSG00000117751.13	2454.98860	1690.14760	0.53850	7.94010	6.74850	K562	SRSF1
PPP1R8	ENSG00000117751.13	442.01460	955.87150	-1.11270	8.16690	6.53880	HepG2	DDX59
PPP1R8	ENSG00000117751.13	680.63240	1178.64630	-0.79220	7.30810	6.37830	HepG2	EIF2S1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 84 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for PPP1R8 is not available on ENCORE database.

9 RBPs can affect the splicing of PPP1R8 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	PPP1R8	ENSG00000117751.13	chr1	+	28167524	28167745			28165287	28165363	28169696	28169814	-0.08400	0.00000	0.00000	excluded	K562	AQR
RI	PPP1R8	ENSG00000117751.13	chr1	+	28176603	28178187			28176603	28176925	28178050	28178187	-0.18200	0.00000	0.00000	excluded	K562	AQR
MXE	PPP1R8	ENSG00000117751.13	chr1	+	28169696	28169841	28173538	28173603	28165209	28165363	28176603	28178039	0.12200	0.00000	0.00000	1included	K562	AQR
MXE	PPP1R8	ENSG00000117751.13	chr1	+	28169696	28169841	28173471	28173603	28165209	28165363	28176603	28178039	0.14200	0.00000	0.00000	1included	K562	AQR
MXE	PPP1R8	ENSG00000117751.13	chr1	+	28169696	28169846	28173538	28173603	28165209	28165363	28176603	28178183	0.12000	0.00000	0.00000	1included	K562	AQR
MXE	PPP1R8	ENSG00000117751.13	chr1	+	28169696	28169846	28173471	28173603	28165209	28165363	28176603	28178183	0.14000	0.00000	0.00000	1included	K562	AQR
MXE	PPP1R8	ENSG00000117751.13	chr1	+	28167524	28167745	28169696	28169814	28165287	28165363	28173538	28173603	-0.11600	0.00000	0.00000	2included	K562	AQR
MXE	PPP1R8	ENSG00000117751.13	chr1	+	28169696	28169841	28173538	28173603	28167524	28167745	28176603	28178187	-0.29200	0.00001	0.00022	2included	HepG2	SFPQ
SE	PPP1R8	ENSG00000117751.13	chr1	+	28167524	28167745			28165287	28165363	28169696	28169841	-0.06100	0.00004	0.01307	excluded	K562	EIF3G
RI	PPP1R8	ENSG00000117751.13	chr1	+	28176603	28178187			28176603	28176925	28178050	28178187	-0.39500	0.00007	0.00327	excluded	K562	HNRNPC

Showing 1 to 10 of 19 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

PPP1R8

General Information

Functions

Localization(RBPImage: PPP1R8)

Domains (AlphaFold: PPP1R8)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for PPP1R8 is not avaliable on ENCORE database.

35 proteins are bound to PPP1R8 RNA:

eCLIP assay for PPP1R8 is not avaliable on ENCORE database.

Diffrential Expression

942 differentially expressed genes after Knockdown/Knockout PPP1R8 protein :

79 RBPs can affect the gene expression of PPP1R8 :

The Knockdown/knockout RNA-Seq assay for PPP1R8 is not available on ENCORE database.

9 RBPs can affect the splicing of PPP1R8 :

The Knockdown/knockout RNA-Seq assay for PPP1R8 is not avaliable on ENCORE database.