CACTIN

General Information

Gene Name       : CACTIN
Gene ID             : ENSG00000105298.13
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CACTIN)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CACTINENSG00000105298.131.183.212.19ENCSR000AEF
HepG2CACTINENSG00000105298.1319.233.0611.14ENCSR985KAT
K562CACTINENSG00000105298.1314.716.4610.59ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
eCLIPeCLIP: 4190ENCSR150QBSCACTINHepG2ReleasedENCAB389VTIENCSR858DLD  
eCLIPeCLIP: 4158ENCSR145KZOCACTINK562ReleasedENCAB389VTIENCSR237DZX  
knockdown followed by RNA-seq CACTIN-BGKcLV23-K562ENCSR416WTECACTINK562ReleasedENCSR877JEM   CRISPRKD% = 61.8,53.6
knockdown followed by RNA-seq CACTIN-BGHcLV22-HepG2ENCSR865WQRCACTINHepG2ReleasedENCSR704VPL   CRISPRKD% = 56.2,57.9






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
CACTIN4190HepG2ENCSR150QBSENCSR858DLDENCAB389VTIA303-349A1ReleasedENCODE4
CACTIN4158K562ENCSR145KZOENCSR237DZXENCAB389VTIA303-349A1ReleasedENCODE4


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CACTINENCAB389VTIA303-349A1BGHcLV22-3, Released, ENCODE4BGKcLV23-27, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CACTINK562CRISPRBGKcLV21-3,BGKcLV21-4BGKcLV21-1,BGKcLV21-216.3,18.0NotSatisfiedENCODE4
CACTINK562CRISPRENCSR416WTEENCSR877JEMCACTIN-BGKcLV23-27,CACTIN-BGKcLV23-28NT-BGKcLV23-1,NT-BGKcLV23-261.8,53.6ReleasedENCODE4
CACTINHepG2CRISPRENCSR865WQRENCSR704VPLCACTIN-BGHcLV22-3,CACTIN-BGHcLV22-4NT-BGHcLV22-1,NT-BGHcLV22-256.2,57.9ReleasedENCODE4
CACTINHepG2CRISPRBGHcLV22-5,BGHcLV22-6BGHcLV22-1,BGHcLV22-249.9,51.6NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CACTINProduct_ID: A303-349A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CACTIN-human		 CACTIN-HEPG2-CRISPR-A303-349A.png<br>Caption: Western blot following CRISPR against CACTIN in HepG2 whole cell lysate using CACTIN specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CACTIN. CACTIN protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 CACTIN-K562-CRISPR-A303-349A.png<br>Caption: Western blot following CRISPR against CACTIN in K562 whole cell lysate using CACTIN specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CACTIN. CACTIN protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CACTIN is not avaliable on ENCORE database.



Proteins bound to RNA
  • CACTIN RNA is bound by 7 proteins.  







eCLIP assay for CACTIN is not avaliable on ENCORE database.





7 proteins are bound to CACTIN RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1936120313612093-3'-UTR,CDS,intron-Distal3.8730810.25633K562RBM15
chr1936118613611877-3'-UTR,intron-Distal,intron-Proximal4.874885.69951K562AQR
chr1936119893612031-3'-UTR,CDS,intron-Distal3.356876.19583K562RBM15
chr1936134903613586-3'-UTR,CDS,intron-Distal,intron-Proximal3.525913.39477K562BUD13
chr1936123303612380-3'-UTR,CDS,intron-Distal,intron-Proximal3.754153.93112K562RBM15
chr1936109353610978-3'-UTR,CDS,intron-Distal3.912383.48187K562RBM15
chr1936118813611917-3'-UTR,5'-UTR,intron-Distal,intron-Proximal4.018593.00996K562BUD13
chr1936118973611976-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.447925.89284K562RBM15
chr1936118773611902-3'-UTR,5'-UTR,intron-Distal,intron-Proximal4.442343.11721K562AQR
chr1936241033624161-5'-UTR,CDS,intron-Distal,intron-Proximal4.563894.21675K562ZNF622
chr1936266033626686-5'-UTR,CDS,intron-Distal3.713035.42720K562UCHL5
chr1936202373620319-CDS,intron-Distal,intron-Proximal5.6973710.84943K562AQR
chr1936202153620270-CDS,intron-Distal4.570804.07072K562UCHL5
chr1936188843618942-CDS,intron-Distal3.248654.14618K562ZNF622
chr1936188743618959-CDS,intron-Distal3.670073.22245K562GRWD1
chr1936188183618874-CDS,intron-Distal,intron-Proximal4.183716.23016HepG2PRPF8
chr1936201243620179-CDS,intron-Distal,intron-Proximal3.225913.70975K562UCHL5
chr1936207123620801-CDS,intron-Distal,intron-Proximal3.087915.46976K562UCHL5
chr1936237023623823-CDS,intron-Distal4.424717.87612K562UCHL5
chr1936202053620270-CDS,intron-Distal4.401644.67744K562BUD13
chr1936206873620714-CDS,intron-Distal,intron-Proximal3.120414.60947K562AQR
chr1936129473613014-intron-Distal,intron-Proximal3.376693.99908K562BUD13
chr1936200833620102-intron-Distal,intron-Proximal5.027316.44303K562AQR
chr1936188243618873-intron-Distal,intron-Proximal3.388894.88100K562AQR
chr1936201023620123-intron-Distal,intron-Proximal4.831155.65342K562AQR
chr1936206573620687-intron-Distal,intron-Proximal3.192587.52050K562AQR
chr1936206063620657-intron-Distal,intron-Proximal3.844385.70278K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CACTIN is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CACTIN is not available on ENCORE database.

  • 87 RBPs can affect the levels of CACTIN.  








The Knockdown/knockout RNA-Seq assay for CACTIN is not available on ENCORE database.





87 RBPs can affect the gene expression of CACTIN :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CACTINENSG00000105298.9408.29150322.276200.341202.146201.59640HepG2ACO1
CACTINENSG00000105298.9327.00890209.660200.641202.873402.33180HepG2AKAP1
CACTINENSG00000105298.9314.93480176.425000.835902.988702.01120HepG2BCCIP
CACTINENSG00000105298.9427.20380322.188500.406902.965702.28180HepG2CKAP4
CACTINENSG00000105298.9183.04550108.845000.749802.506501.78120HepG2CNOT7
CACTINENSG00000105298.9267.37490183.998600.539103.090602.41890HepG2CELF1
CACTINENSG00000105298.9279.09260200.799100.474901.839101.31750HepG2DDX3X
CACTINENSG00000105298.9177.4874085.139901.059707.118206.14050HepG2DNAJC2
CACTINENSG00000105298.9377.48730183.202101.042904.870204.02600HepG2EIF4A3
CACTINENSG00000105298.9404.21010184.978301.127609.600108.38040HepG2FXR1
CACTINENSG00000105298.9270.73160171.523400.658403.151402.44320HepG2G3BP1
CACTINENSG00000105298.9196.38970357.77030-0.865305.478604.59840HepG2GPKOW
CACTINENSG00000105298.9172.48190100.793900.774902.822701.69940HepG2GRSF1
CACTINENSG00000105298.9165.6806090.040700.879703.336301.79840HepG2HNRNPAB
CACTINENSG00000105298.9327.10940173.848800.911806.004505.00410HepG2HNRNPK
CACTINENSG00000105298.9241.18300180.195300.420502.054701.46300HepG2HNRNPL
CACTINENSG00000105298.9249.09330341.42860-0.454902.028501.48100HepG2HNRNPLL
CACTINENSG00000105298.9202.8908095.802901.082505.059104.25940HepG2HNRNPU
CACTINENSG00000105298.9281.83140174.324500.693003.032702.33100HepG2IGF2BP2
CACTINENSG00000105298.9164.1164097.858100.745903.917803.06110HepG2ILF2
CACTINENSG00000105298.9186.72690103.901800.845603.297501.96940HepG2KHDRBS1
CACTINENSG00000105298.9220.38040102.678001.101802.933101.76600HepG2LARP7
CACTINENSG00000105298.9462.09250185.043401.3202013.3168011.92410HepG2MAGOH
CACTINENSG00000105298.9373.94830186.057101.007006.833905.87520HepG2MATR3
CACTINENSG00000105298.9334.16780190.422000.811304.467703.67180HepG2NCBP2
CACTINENSG00000105298.9381.72560196.162000.960409.753608.34970HepG2NOL12
CACTINENSG00000105298.9219.61790121.807400.850303.423101.81300HepG2NOLC1
CACTINENSG00000105298.9347.50920159.401801.124305.008003.51440HepG2NONO
CACTINENSG00000105298.9366.48310178.313701.0392011.2589010.16830HepG2PCBP1
CACTINENSG00000105298.9220.2037085.447101.3656011.4728010.02900HepG2PPIL4
CACTINENSG00000105298.9268.90760181.448600.567403.025302.45840HepG2PTBP1
CACTINENSG00000105298.9314.68240187.259500.748805.033104.25860HepG2PUF60
CACTINENSG00000105298.9440.79550198.790901.1488017.3334016.11250HepG2PUM1
CACTINENSG00000105298.9642.57370410.421700.646706.631605.91270HepG2RBM22
CACTINENSG00000105298.9318.67870123.832001.363603.695702.53990HepG2RBM27
CACTINENSG00000105298.9218.75660100.558901.121202.410501.42290HepG2RCC2
CACTINENSG00000105298.9372.23730201.928400.882307.722006.89920HepG2NELFE
CACTINENSG00000105298.9317.87340188.080600.757005.607704.76850HepG2RECQL
CACTINENSG00000105298.9259.88440158.948800.709202.983202.34890HepG2RPS19
CACTINENSG00000105298.9274.81470114.172801.267104.258403.27290HepG2RPS2
CACTINENSG00000105298.9205.06720109.477200.905403.843102.89940HepG2RPS3A
CACTINENSG00000105298.9200.2334085.311701.230809.124408.06200HepG2SAFB2
CACTINENSG00000105298.9192.48550103.086900.900803.682202.17840HepG2SART3
CACTINENSG00000105298.9979.50600390.480801.3267016.1131014.41050HepG2SF3A3
CACTINENSG00000105298.9342.69110188.897500.859207.164806.28490HepG2SF3B4
CACTINENSG00000105298.9287.10780179.566400.677004.954104.05770HepG2SLBP
CACTINENSG00000105298.9242.0397099.479401.2827011.4296010.26170HepG2SMN1
CACTINENSG00000105298.9242.18060109.835801.140604.146502.95640HepG2SMNDC1
CACTINENSG00000105298.9219.95390376.01640-0.773603.718502.80750HepG2SNRNP70
CACTINENSG00000105298.9395.91490184.899501.098307.362506.40820HepG2SRSF5
CACTINENSG00000105298.9287.18840402.43720-0.486803.723603.14820HepG2SSB
CACTINENSG00000105298.9170.4880095.294900.839102.425901.40490HepG2STIP1
CACTINENSG00000105298.9407.27670210.274800.9536011.1518010.04630HepG2SUCLG1
CACTINENSG00000105298.997.11630177.24320-0.867904.222503.55160HepG2TAF15
CACTINENSG00000105298.9177.0848093.058300.928102.873202.36570HepG2TIAL1
CACTINENSG00000105298.9272.7267093.924701.5378017.0677015.82670HepG2TRIM56
CACTINENSG00000105298.9326.58680182.955500.835902.386401.57920HepG2TUFM
CACTINENSG00000105298.9539.00160191.289901.494405.361103.94960HepG2U2AF2
CACTINENSG00000105298.9374.37980193.662700.950903.657602.68840HepG2UBE2L3
CACTINENSG00000105298.9175.42850101.979200.782503.013302.22320HepG2UCHL5
CACTINENSG00000105298.9450.87460334.335700.431302.328001.76790HepG2UTP3
CACTINENSG00000105298.9184.8799098.069000.914602.629301.56780HepG2XPO5
CACTINENSG00000105298.9267.26630169.973700.652902.240801.58250HepG2XRN2
CACTINENSG00000105298.9916.12780717.966000.351503.476802.85380K562AGGF1
CACTINENSG00000105298.91049.57830788.945200.411703.548502.40150K562AKAP8
CACTINENSG00000105298.91001.00980747.078400.422003.548802.80560K562AKAP8L
CACTINENSG00000105298.93045.443901031.822101.561409.216008.24600K562AQR
CACTINENSG00000105298.9996.86930783.869300.346703.370102.35270K562ATP5C1
CACTINENSG00000105298.9571.94300369.117000.631702.678901.73410K562BCLAF1
CACTINENSG00000105298.91177.073701543.46530-0.391004.442203.19960K562CELF1
CACTINENSG00000105298.91284.202301091.080300.235002.112601.53060K562DDX47
CACTINENSG00000105298.9959.05270803.895700.254502.091101.34090K562DKC1
CACTINENSG00000105298.9820.22100327.884001.3227017.8682015.35890K562EFTUD2
CACTINENSG00000105298.9293.20160165.944400.821105.606904.48970K562EIF3G
CACTINENSG00000105298.9797.71140503.420700.664006.173005.32240K562FXR1
CACTINENSG00000105298.924.9981092.89230-1.893706.285205.12170K562G3BP2
CACTINENSG00000105298.9451.89680239.652700.914902.793401.76340K562IGF2BP2
CACTINENSG00000105298.9587.56570251.653801.2232017.6940015.41920K562MAGOH
CACTINENSG00000105298.9381.39680251.111000.602902.340601.49840K562MATR3
CACTINENSG00000105298.9997.791701207.53240-0.275303.301002.49220K562NSUN2
CACTINENSG00000105298.9443.59550223.870900.986506.843505.28410K562PCBP2
CACTINENSG00000105298.9818.98230319.073901.3598011.7469010.19090K562PRPF8
CACTINENSG00000105298.9516.30160244.513201.0782014.0351012.59990K562PTBP1
CACTINENSG00000105298.91101.882701456.77080-0.402804.417103.04280K562PUM1
CACTINENSG00000105298.9909.58030721.889900.333302.845902.24530K562RBM15
CACTINENSG00000105298.9584.28210234.343301.3179015.8007013.60160K562RBM22
CACTINENSG00000105298.9532.00050324.869400.711503.132202.26020K562RPS19
CACTINENSG00000105298.9432.08370270.508100.675502.714901.72100K562RPS3
CACTINENSG00000105298.9258.0090096.489001.4189010.399708.69250K562SF3B4
CACTINENSG00000105298.9536.63380374.763300.517902.382401.44840K562SRFBP1
CACTINENSG00000105298.9462.68220239.728200.948509.527008.22520K562SRSF1
CACTINENSG00000105298.9476.35920261.137200.867107.498605.63460K562SRSF4
CACTINENSG00000105298.9385.89170226.464100.768803.319502.15050K562SRSF5
CACTINENSG00000105298.9580.05070812.21130-0.485703.747502.14370K562SSRP1
CACTINENSG00000105298.9633.00250242.569401.383708.585707.25060K562TARDBP
CACTINENSG00000105298.9493.25540233.999001.0757012.6832010.80920K562TIA1
CACTINENSG00000105298.9447.40550229.743900.961504.381802.95360K562TRA2A
CACTINENSG00000105298.91147.221401544.14620-0.428704.703302.87920K562UBE2L3
CACTINENSG00000105298.9791.981001095.56980-0.468104.672603.52070K562WDR3
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CACTIN is not available on ENCORE database.





3 RBPs can affect the splicing of CACTIN :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECACTINENSG00000105298.9chr19-3613783361385836135043613584361439436145870.145000.000010.00014includedK562AQR
SECACTINENSG00000105298.9chr19-3619347361954436190773619240362012436202700.141000.000010.00118includedK562HNRNPU
SECACTINENSG00000105298.9chr19-3619347361954436190773619240362012436202700.153000.000140.00571includedK562AGGF1
A3SSCACTINENSG00000105298.9chr19-3620124362037036201243620270362070436208890.247000.000000.00000longerK562AQR
A3SSCACTINENSG00000105298.9chr19-3620704362088936207043620800362368536241600.293000.000000.00000longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CACTIN is not avaliable on ENCORE database.