EWSR1

General Information

Gene Name : EWSR1
Gene ID : ENSG00000182944.17
Essential Gene : No

Functions

Splicing regulation

Localization(RBPImage: EWSR1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Variable Nuclear Intensity
Nuclear release mitosis

Domains (AlphaFold: EWSR1)

RRM

Antibody Validation

Bethyl Labs: A300-417A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	EWSR1	ENSG00000182944.17	271.62	326.61	299.12	ENCSR000AEF
HepG2	EWSR1	ENSG00000182944.17	244.44	218.55	231.50	ENCSR985KAT
K562	EWSR1	ENSG00000182944.17	270.84	302.39	286.62	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 440	ENCSR887LPK	EWSR1	K562	Released	ENCAB366VTK	ENCSR067UKU
knockdown followed by RNA-seq	EWSR1-BGKLV08-K562	ENCSR831YGP	EWSR1	K562	Released		ENCSR913CAE	shRNA	KD% = 87.7,83.3
knockdown followed by RNA-seq	EWSR1-BGHLV31-HepG2	ENCSR532ZPP	EWSR1	HepG2	Released		ENCSR538QOG	shRNA	KD% = 95.0,96.6
RNA Bind-n-Seq	RBNS: EWSR1	ENCSR063HQO	EWSR1	cell-free sample	Released

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
EWSR1	440	K562	ENCSR887LPK	ENCSR067UKU	ENCAB366VTK	A300-417A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
EWSR1	ENCAB366VTK	A300-417A	1			Released, ENCODE3	BGHLV31-35, Released, ENCODE3	BGKLV08-25, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
EWSR1	K562	shRNA	ENCSR831YGP	ENCSR913CAE	EWSR1-LV08-25,EWSR1-LV08-26	Nontarget-LV08-1_00003527,nontarget-LV08-2_00003527	87.7,83.3	Released	ENCODE3
EWSR1	HepG2	shRNA			BGHLV12-25,BGHLV12-26	BGHLV12-1,BGHLV12-2		NotSatisfied	ENCODE3
EWSR1	HepG2	shRNA			BGHLV26-11,BGHLV26-12	BGHLV26-1,BGHLV26-2	redo transfection,redo transfection	NotSatisfied	ENCODE3
EWSR1	HepG2	shRNA			BGHLV27-17,BGHLV27-18	BGHLV27-1,BGHLV27-2		NotSatisfied	ENCODE3
EWSR1	HepG2	shRNA			BGHLV30-37,BGHLV30-38	BGHLV30-1,BGHLV30-2	0.0,0.0	NotSatisfied	ENCODE3
EWSR1	HepG2	shRNA	ENCSR532ZPP	ENCSR538QOG	EWSR1_BGHLV31-35,EWSR1_BGHLV31-36	NT-BGHLV31-1,NT-BGHLV31-2	95.0,96.6	Released	ENCODE3
EWSR1	HepG2	shRNA			BGHLV33-13,BGHLV33-14	BGHLV33-1,BGHLV33-2		NotSatisfied	ENCODE3

RNA Bind-n-Seqn

GeneName	gene_aliases	Status	Project	Note
EWSR1	EWSR1		Released	ENCODE3

Localization Image in HepG2

EWSR1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Variable Nuclear Intensity

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
EWSR1	Product_ID: A300-417A Lot_ID: 1 Source: Bethyl Labs Target Name: EWSR1-human

RNA Targets

EWSR1 protein binds to 8,710 RNA regions.

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The binding motifs of EWSR1

Proteins bound to RNA

EWSR1 RNA is bound by 48 proteins.

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EWSR1 protein has 8,710 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
EWSR1	chr19	2270161	2270240	+	7.37911	86.36328	K562	OAZ1	intron-Distal
EWSR1	chr19	2270240	2270292	+	6.95268	65.31644	K562	OAZ1	5'-UTR,intron-Distal
EWSR1	chr16	85648614	85648710	+	4.54729	32.49518	K562	GSE1	intron-Distal
EWSR1	chr16	1422439	1422531	-	4.74973	25.87011	K562	UNKL	intron-Distal
EWSR1	chr22	49961687	49961781	-	4.88652	23.89154	K562	C22orf34	intron-Distal
EWSR1	chr13	92000858	92000928	+	3.97776	23.43094	K562	MIR17HG	intron-Distal
EWSR1	chr16	591277	591341	+	4.27771	21.38535	K562	CAPN15	intron-Distal
EWSR1	chr16	88558883	88558964	+	5.81335	20.59806	K562	ZFPM1	intron-Distal
EWSR1	chr4	1240155	1240227	-	4.68477	19.63075	K562	CTBP1	intron-Distal
EWSR1	chr16	88570827	88570966	+	4.37131	18.04847	K562	ZFPM1	intron-Distal

Showing 1 to 10 of 100 entries

48 proteins are bound to EWSR1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr22	29685451	29685504	+	intron-Distal	4.99962	400.00000	HepG2	PCBP2
chr22	29685504	29685561	+	intron-Distal	5.48152	84.72907	HepG2	PCBP2
chr22	29696423	29696463	+	3'-UTR	5.89184	52.87352	K562	LIN28B
chr22	29664396	29664519	+	intron-Distal,intron-Proximal	5.16810	51.87906	HepG2	EFTUD2
chr22	29687537	29687585	+	CDS,intron-Distal,intron-Proximal	3.74928	41.42353	HepG2	U2AF2
chr22	29696384	29696423	+	3'-UTR	4.98907	31.12939	K562	LIN28B
chr22	29664519	29664687	+	intron-Distal	4.94742	28.88452	HepG2	EFTUD2
chr22	29695807	29695832	+	CDS	5.34272	28.75253	K562	UCHL5
chr22	29687002	29687161	+	intron-Distal	3.80622	27.38921	HepG2	KHSRP
chr22	29664384	29664492	+	intron-Distal,intron-Proximal	4.67568	27.27656	K562	EFTUD2
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 226 entries

No autoregularoty interactions between protein EWSR1 and its RNA.

Diffrential Expression

5,967 differentially expressed genes after Knockdown/Knockout EWSR1 protein.

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80 RBPs can affect the levels of EWSR1.

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5,967 differentially expressed genes after Knockdown/Knockout EWSR1 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CRLF1	ENSG00000006016.6	1681.85720	4869.86520	-1.53380	60.41980	57.24860	HepG2	EWSR1
IL32	ENSG00000008517.12	5144.24770	2399.56260	1.10010	32.93640	30.43030	HepG2	EWSR1
JHDM1D	ENSG00000006459.6	1122.33860	2360.73430	-1.07270	26.46990	24.18090	HepG2	EWSR1
TNFRSF12A	ENSG00000006327.9	1739.47350	793.64880	1.13200	26.12580	23.84030	HepG2	EWSR1
DPEP1	ENSG00000015413.5	51.12060	253.73340	-2.31130	25.14110	22.87970	HepG2	EWSR1
DBNDD1	ENSG00000003249.9	1669.43990	829.28590	1.00930	21.01530	18.90000	HepG2	EWSR1
SLC7A2	ENSG00000003989.12	4340.72110	7155.06600	-0.72100	15.87540	13.97280	HepG2	EWSR1
CLCN6	ENSG00000011021.17	1104.65390	1972.94380	-0.83680	14.06490	12.26350	HepG2	EWSR1
NCAPD2	ENSG00000010292.8	2420.41630	3938.45470	-0.70240	13.87930	12.08600	HepG2	EWSR1
ARHGAP33	ENSG00000004777.14	130.83500	322.35330	-1.30090	12.86890	11.12190	HepG2	EWSR1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

80 RBPs can affect the gene expression of EWSR1 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
EWSR1	ENSG00000182944.13	7019.62830	4655.31620	0.59240	21.31250	19.88440	HepG2	PCBP1
EWSR1	ENSG00000182944.13	2675.13290	4575.06950	-0.77420	22.68760	18.93250	K562	EWSR1
EWSR1	ENSG00000182944.13	4626.85810	8022.52840	-0.79400	17.64580	16.21270	HepG2	RPLP0
EWSR1	ENSG00000182944.13	8080.49350	4537.82530	0.83230	16.85560	15.28080	HepG2	HNRNPK
EWSR1	ENSG00000182944.13	6704.39630	9870.39730	-0.55800	15.99050	14.95370	HepG2	RBM22
EWSR1	ENSG00000182944.13	7146.49670	4910.49970	0.54130	15.58130	14.31310	HepG2	RECQL
EWSR1	ENSG00000182944.13	4484.94210	7441.89880	-0.73060	15.32510	14.07620	HepG2	PUF60
EWSR1	ENSG00000182944.13	9288.05860	15825.53220	-0.76880	15.48850	13.53800	K562	PUF60
EWSR1	ENSG00000182944.13	7432.82750	5147.69920	0.52990	13.72420	12.84330	HepG2	SRSF1
EWSR1	ENSG00000182944.13	5284.45350	7361.44520	-0.47820	13.45850	11.94790	HepG2	PRPF4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 90 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

101 alternative splicing events after Knockdown/Knockout EWSR1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	ZNF207	ENSG00000010244.12	chr17	+	30688486	30688534			30687910	30687986	30689932	30690003	-0.08900	0.01785	excluded	K562	EWSR1
SE	MPP1	ENSG00000130830.10	chrX	-	154012302	154012383			154011701	154011782	154013325	154013432	-0.05400	0.02446	excluded	K562	EWSR1
SE	ADAMTS14	ENSG00000138316.6	chr10	+	72510894	72511032			72509621	72509741	72511233	72511402	-0.13900	0.01785	excluded	K562	EWSR1
SE	PNISR	ENSG00000132424.10	chr6	-	99864224	99864304			99862447	99862566	99873090	99873184	-0.22600	0.00217	excluded	K562	EWSR1
RI	GIGYF1	ENSG00000146830.9	chr7	-	100283596	100284056			100283596	100283702	100283802	100284056	0.17500	0.00167	included	K562	EWSR1
RI	FKBP1A	ENSG00000088832.10	chr20	-	1349621	1350716			1349621	1349683	1350415	1350716	-0.15600	0.00674	excluded	K562	EWSR1
MXE	ADK	ENSG00000156110.9	chr10	+	75960521	75960596	75984295	75984349	75910959	75911101	76074424	76074503	0.24000	0.00031	1included	K562	EWSR1
MXE	RANBP1	ENSG00000099901.12	chr22	+	20112830	20112959	20113825	20113891	20109786	20109944	20114477	20114558	0.12000	0.00000	1included	K562	EWSR1
MXE	RPS12	ENSG00000112306.7	chr6	+	133136110	133136227	133137599	133137702	133135870	133135921	133138098	133138200	0.15800	0.00000	1included	K562	EWSR1
MXE	RPS12	ENSG00000112306.7	chr6	+	133136110	133136227	133138098	133138200	133135870	133135921	133138618	133138703	0.14300	0.00000	1included	K562	EWSR1

Showing 1 to 10 of 100 entries

41 RBPs can affect the splicing of EWSR1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	EWSR1	ENSG00000182944.13	chr22	+	29684594	29687588	29682911	29683123	29688125	29688158	-0.13500	0.00000	excluded	K562	SRSF1
SE	EWSR1	ENSG00000182944.13	chr22	+	29678378	29678546	29674018	29674205	29682911	29683123	-0.05200	0.00001	excluded	HepG2	U2AF1
SE	EWSR1	ENSG00000182944.13	chr22	+	29693816	29693939	29692228	29692358	29694722	29694885	-0.10600	0.00000	excluded	HepG2	U2AF1
SE	EWSR1	ENSG00000182944.13	chr22	+	29678378	29678546	29674018	29674205	29682911	29683123	-0.17700	0.00000	excluded	HepG2	U2AF2
SE	EWSR1	ENSG00000182944.13	chr22	+	29693816	29693973	29691514	29692358	29694722	29694885	-0.07100	0.00000	excluded	K562	AQR
A3SS	EWSR1	ENSG00000182944.13	chr22	+	29696092	29696333	29696111	29696333	29695588	29695841	0.05400	0.00000	longer	K562	AQR
A3SS	EWSR1	ENSG00000182944.13	chr22	+	29668344	29668420	29668374	29668420	29668204	29668241	0.69500	0.00000	longer	K562	AQR
RI	EWSR1	ENSG00000182944.13	chr22	+	29684594	29687588	29684594	29684775	29687550	29687588	-0.18400	0.00014	excluded	HepG2	AKAP1
RI	EWSR1	ENSG00000182944.13	chr22	+	29694722	29695270	29694722	29694885	29695184	29695270	0.53500	0.00000	included	K562	PUS1
RI	EWSR1	ENSG00000182944.13	chr22	+	29694722	29695270	29694722	29694885	29695184	29695270	0.41800	0.00000	included	K562	AGGF1

Showing 1 to 10 of 83 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The EWSR1 protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
RI	EWSR1	ENSG00000182944.13	chr22	+	29684594	29687588			29684594	29684775	29687553	29687588	0.21500	0.00004	0.03143	included	K562	EWSR1

Showing 1 to 1 of 1 entries