EWSR1 antibody: A300-417A


Antibody Info
Product_IDA300-417A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB366VTK
Target Info
GeneNameEWSR1
Phosphate
Target_nameEWSR1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 EWSR1-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against EWSR1 in HepG2 whole cell lysate using EWSR1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EWSR1.EWSR1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against EWSR1 in HepG2 whole cell lysate using EWSR1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EWSR1.EWSR1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV31-35

Status: Released

Lab: Graveley Lab

K562 Bethyl_A300-417A_1_EWSR1_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EWSR1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-EWSR1 antibody (lanes under 'EWSR1') or using rabbit normal IgG (lanes under 'IgG').

Caption: IP-Western Blot analysis of K562 whole cell lysate using EWSR1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-EWSR1 antibody (lanes under 'EWSR1') or using rabbit normal IgG (lanes under 'IgG').

Method: immunoprecipitation

Releated Sample: eCLIP:440

Status: Released

Lab: Yeo Lab

EWSR1-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against EWSR1 in K562 whole cell lysate using EWSR1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EWSR1.EWSR1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against EWSR1 in K562 whole cell lysate using EWSR1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EWSR1.EWSR1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-25

Status: Released

Lab: Graveley Lab