GSK3B

General Information

Gene Name       : GSK3B
Gene ID             : ENSG00000082701.15
Essential Gene : No

Functions

Localization

Domains (AlphaFold: GSK3B)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878GSK3BENSG00000082701.1527.3428.2527.80ENCSR000AEF
HepG2GSK3BENSG00000082701.1518.6921.2219.95ENCSR985KAT
K562GSK3BENSG00000082701.1515.0315.5715.30ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq GSK3B-BGKcLV15-K562ENCSR025XBHGSK3BK562ReleasedENCSR404OHQ   CRISPRKD% = 72.0,65.0
knockdown followed by RNA-seq GSK3B-BGHcLV16-HepG2ENCSR426JCDGSK3BHepG2ReleasedENCSR964HKT   CRISPRKD% = 54.9,61.4






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
GSK3AENCAB957NOPMA3-038RF227934BGHcLV16-31, Released, ENCODE4BGKcLV15-37, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
GSK3BK562CRISPRENCSR025XBHENCSR404OHQGSK3B-BGKcLV15-37,GSK3B-BGKcLV15-38NT-BGKcLV15-1,NT-BGKcLV15-272.0,65.0ReleasedENCODE4
GSK3BHepG2CRISPRENCSR426JCDENCSR964HKTGSK3B-BGHcLV16-31,GSK3B-BGHcLV16-32NT-BGHcLV16-1,NT-BGHcLV16-254.9,61.4ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GSK3AProduct_ID: MA3-038
Lot_ID: RF227934
Source: Thermo Fisher
Target Name: GSK3A-human		 GSK3beta-HEPG2-CRISPR-MA3-038.png<br>Caption: Western blot following CRISPR against GSK3B in HepG2 whole cell lysate using GSK3B specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3B. GSK3B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 GSK3beta-K562-CRISPR-MA3-038.png<br>Caption: Western blot following CRISPR against GSK3B in K562 whole cell lysate using GSK3B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3B. GSK3B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for GSK3B is not avaliable on ENCORE database.



Proteins bound to RNA
  • GSK3B RNA is bound by 25 proteins.  







eCLIP assay for GSK3B is not avaliable on ENCORE database.





25 proteins are bound to GSK3B RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr3119543626119543664-3'-UTR,intron-Distal3.826863.12080HepG2XPO5
chr3119544912119545002-3'-UTR,intron-Distal3.456784.21448HepG2UPF1
chr3119543120119543187-3'-UTR,intron-Distal3.248574.91593K562FAM120A
chr3119542826119542877-3'-UTR,intron-Distal4.9171312.31980K562PUM2
chr3119540839119540890-3'-UTR,intron-Distal4.917136.68732K562PUM2
chr3119545439119545505-3'-UTR,intron-Distal,intron-Proximal4.205343.35973HepG2FUBP3
chr3119544906119544969-3'-UTR,intron-Distal3.567283.88742K562FAM120A
chr3119542931119542990-3'-UTR,intron-Distal3.413853.66308K562UPF1
chr3119540840119540896-3'-UTR,intron-Distal4.732563.83835HepG2FUBP3
chr3119542938119542990-3'-UTR,intron-Distal3.672573.37207K562FAM120A
chr3119545336119545406-3'-UTR,intron-Distal3.810005.10007K562FAM120A
chr3119541166119541271-3'-UTR,intron-Distal5.288746.43721K562PUM2
chr3119543116119543198-3'-UTR,intron-Distal3.6894911.26390K562TARDBP
chr3119540890119540923-3'-UTR,intron-Distal4.143996.21733K562PUM2
chr3119542952119542994-3'-UTR,intron-Distal4.064518.57166HepG2FUBP3
chr3119543706119543834-3'-UTR,intron-Distal3.764426.18168K562FAM120A
chr3119545345119545418-3'-UTR,intron-Distal3.429603.85636HepG2FAM120A
chr3119813211119813260-5'-UTR,intron-Distal3.7573411.34202HepG2DDX3X
chr3119813219119813261-5'-UTR,intron-Distal4.072955.47892K562DDX3X
chr3119812936119813040-5'-UTR,intron-Distal4.297084.48994K562RBM15
chr3119812669119812805-5'-UTR,intron-Distal4.5627324.13590HepG2PCBP2
chr3119812479119812504-5'-UTR,intron-Distal3.498593.27569HepG2BCLAF1
chr3119812912119812999-5'-UTR,intron-Distal3.0326815.22068HepG2DDX3X
chr3119813157119813262-5'-UTR,intron-Distal3.929048.37300HepG2NCBP2
chr3119813004119813137-5'-UTR,intron-Distal3.9170831.96003HepG2DDX3X
chr3119720986119721087-CDS,intron-Distal,intron-Proximal3.291078.02162HepG2BCLAF1
chr3119666105119666180-CDS,intron-Distal,intron-Proximal3.337624.74886HepG2BCLAF1
chr3119720888119720963-CDS,intron-Distal,intron-Proximal3.339634.26492HepG2BCLAF1
chr3119631513119631575-CDS,intron-Distal,intron-Proximal4.741443.70131HepG2PRPF8
chr3119631556119631613-CDS,intron-Distal3.359823.57857HepG2BCLAF1
chr3119721004119721065-CDS,intron-Distal4.372794.94878HepG2GRWD1
chr3119812201119812255-CDS,intron-Distal4.454834.82934K562RBM15
chr3119634888119634941-CDS,intron-Distal,intron-Proximal3.867413.34184HepG2GRWD1
chr3119624589119624656-CDS,intron-Distal,intron-Proximal3.788664.51739HepG2BCLAF1
chr3119634889119634926-CDS,intron-Distal,intron-Proximal3.406383.68471HepG2BCLAF1
chr3119640996119641085-intron-Distal4.701344.45977HepG2U2AF2
chr3119810974119811057-intron-Distal3.770073.26999K562KHSRP
chr3119622361119622468-intron-Distal3.292263.68839K562KHSRP
chr3119641004119641081-intron-Distal5.3548710.20560HepG2PTBP1
chr3119811893119811978-intron-Distal4.088784.31512K562KHSRP
chr3119604596119604625-intron-Distal4.258273.89080K562EXOSC5
chr3119662442119662648-intron-Distal4.804837.02715K562MATR3
chr3119765174119765238-intron-Distal3.399103.97172HepG2QKI
chr3119793101119793150-intron-Distal3.404663.21640HepG2ILF3
chr3119764891119764978-intron-Distal4.144164.60998HepG2PCBP2
chr3119756949119757054-intron-Distal3.902563.16986HepG2QKI
chr3119603636119603677-intron-Distal4.214543.89080K562EXOSC5
chr3119662467119662648-intron-Distal5.0730318.60142K562PTBP1
chr3119640999119641126-intron-Distal4.015765.97588K562PCBP1
chr3119795017119795065-intron-Distal4.299503.89080K562EXOSC5
chr3119604042119604097-intron-Distal3.960466.41110K562EXOSC5
chr3119771598119771641-intron-Distal4.305633.79000HepG2U2AF2
chr3119664737119664843-intron-Distal3.775063.25538K562KHSRP
chr3119711079119711217-intron-Distal3.398244.98593K562KHSRP
chr3119673026119673081-intron-Distal3.656207.26573HepG2PTBP1
chr3119662539119662665-intron-Distal3.931734.94624HepG2MATR3
chr3119662739119662858-intron-Distal4.3517215.16976K562PTBP1
chr3119605328119605388-intron-Distal4.568458.51196K562EXOSC5
chr3119640958119641083-intron-Distal5.4195719.79046HepG2PCBP2
chr3119655541119655766-intron-Distal3.249744.13575K562KHSRP
chr3119662407119662539-intron-Distal4.724213.62238HepG2MATR3
chr3119798073119798169-intron-Distal4.474574.38266K562TARDBP
chr3119602862119602997-intron-Distal4.571615.75114HepG2PTBP1
chr3119600007119600124-intron-Distal4.308124.54401K562KHSRP
chr3119718792119718910-intron-Distal3.054283.33633HepG2MATR3
chr3119637487119637567-intron-Distal3.901393.37414K562CSTF2T
chr3119662763119662857-intron-Distal4.4957613.63692HepG2PTBP1
chr3119637500119637568-intron-Distal3.873453.11181HepG2CSTF2
chr3119662552119662666-intron-Distal3.7137413.79150HepG2PTBP1
chr3119718591119718684-intron-Distal4.667125.07733HepG2PTBP1
chr3119797706119797777-intron-Distal4.054683.91772K562TARDBP
chr3119762317119762373-intron-Distal4.706036.08412HepG2HNRNPM
chr3119793490119793549-intron-Distal4.249733.59917K562EXOSC5
chr3119604965119605015-intron-Distal3.646844.61337K562EXOSC5
chr3119605710119605736-intron-Distal4.155043.30696K562EXOSC5
chr3119662736119662861-intron-Distal3.678723.10961HepG2MATR3
chr3119740801119740878-intron-Distal3.990773.84389HepG2SUGP2
chr3119759518119759564-intron-Distal3.626285.09669HepG2QKI
chr3119624755119624899-intron-Distal,intron-Proximal3.450013.68839K562KHSRP
chr3119662714119662763-intron-Distal3.679625.07626HepG2PTBP1
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for GSK3B is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for GSK3B is not available on ENCORE database.

  • 73 RBPs can affect the levels of GSK3B.  








The Knockdown/knockout RNA-Seq assay for GSK3B is not available on ENCORE database.





73 RBPs can affect the gene expression of GSK3B :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
GSK3BENSG00000082701.101976.017601350.540100.5490012.0930011.01230HepG2ACO1
GSK3BENSG00000082701.102960.090401597.980700.8893011.5714010.55690HepG2AKAP1
GSK3BENSG00000082701.101733.344501356.898900.353104.976803.97490HepG2AKAP8L
GSK3BENSG00000082701.101661.076201322.174700.329102.222501.51440HepG2ASCC1
GSK3BENSG00000082701.103014.128701818.189200.7291022.6968021.07560HepG2AUH
GSK3BENSG00000082701.102642.825801876.108700.494206.039905.19520HepG2BOP1
GSK3BENSG00000082701.101902.329301483.278200.358903.810402.92630HepG2CALR
GSK3BENSG00000082701.101563.457501350.262900.211402.391701.76520HepG2CKAP4
GSK3BENSG00000082701.101213.443901722.42060-0.505303.309002.56430HepG2CPSF6
GSK3BENSG00000082701.101684.908401151.089400.549606.470705.48150HepG2DDX21
GSK3BENSG00000082701.101390.203301126.244200.303702.460901.60370HepG2DDX24
GSK3BENSG00000082701.102306.159101530.701300.591206.177605.27760HepG2DDX3X
GSK3BENSG00000082701.101661.985401315.006200.337701.995601.46330HepG2DDX6
GSK3BENSG00000082701.101151.905301312.20890-0.188001.912301.35160HepG2DNAJC2
GSK3BENSG00000082701.101593.54220960.617600.730108.731707.21600HepG2EIF3D
GSK3BENSG00000082701.102472.574901360.142900.862208.707407.52360HepG2EIF4B
GSK3BENSG00000082701.102877.707902087.438600.4631010.096309.06510HepG2ETF1
GSK3BENSG00000082701.102675.836401770.120500.596006.083704.98550HepG2FIP1L1
GSK3BENSG00000082701.10974.93790756.698400.365502.820902.02680HepG2FUBP3
GSK3BENSG00000082701.101948.770101409.532000.467204.104003.27250HepG2FXR1
GSK3BENSG00000082701.101983.208301307.469600.601005.946704.98690HepG2G3BP1
GSK3BENSG00000082701.101537.676801952.63650-0.344702.237001.65800HepG2GPKOW
GSK3BENSG00000082701.101500.864601824.71500-0.281902.180601.53860HepG2HNRNPF
GSK3BENSG00000082701.101184.611401452.00360-0.293602.484001.84220HepG2HNRNPL
GSK3BENSG00000082701.102412.877601863.424400.372703.200802.53010HepG2HNRNPLL
GSK3BENSG00000082701.101485.179901796.81900-0.274802.485101.79800HepG2IGF2BP1
GSK3BENSG00000082701.101997.255701328.986500.587606.245905.26920HepG2IGF2BP2
GSK3BENSG00000082701.102041.815101247.308300.710902.547201.60580HepG2IGF2BP3
GSK3BENSG00000082701.102896.216802234.672300.374002.330201.57270HepG2LARP4
GSK3BENSG00000082701.101766.262101410.024800.324902.141601.56220HepG2MAGOH
GSK3BENSG00000082701.101773.502801417.892400.322801.924001.39330HepG2MATR3
GSK3BENSG00000082701.102186.231701451.272100.591005.946005.03430HepG2NCBP2
GSK3BENSG00000082701.101895.223601580.486900.261902.200301.51930HepG2NOL12
GSK3BENSG00000082701.101308.774501652.18160-0.336201.956401.32250HepG2NSUN2
GSK3BENSG00000082701.101792.074801292.362300.471503.974503.11960HepG2PABPC4
GSK3BENSG00000082701.101757.073601461.867300.265303.064502.17740HepG2PARN
GSK3BENSG00000082701.10921.293101317.26260-0.515807.501006.33100HepG2PPIL4
GSK3BENSG00000082701.102192.335101307.606800.7454020.6661019.43720HepG2PTBP1
GSK3BENSG00000082701.102355.062401600.274900.557308.636607.48270HepG2PUM2
GSK3BENSG00000082701.101975.752701375.615100.5222011.7241010.60680HepG2PUS1
GSK3BENSG00000082701.102313.409601416.058900.7080015.1448013.84980HepG2QKI
GSK3BENSG00000082701.103239.942701744.730300.8929029.0452027.72080HepG2RBM22
GSK3BENSG00000082701.102113.821901285.206600.7178012.6325011.25520HepG2RBM25
GSK3BENSG00000082701.102092.885001463.168500.516303.435801.73570HepG2RBM47
GSK3BENSG00000082701.102036.871401455.197300.485009.004408.13020HepG2NELFE
GSK3BENSG00000082701.101353.84260934.690900.534406.322205.43390HepG2RPS19
GSK3BENSG00000082701.101729.412401343.894300.363803.264902.39540HepG2RPS2
GSK3BENSG00000082701.10625.09460999.26560-0.676802.866302.06780HepG2RRP9
GSK3BENSG00000082701.10806.439801315.23250-0.7057016.0676014.70750HepG2SAFB2
GSK3BENSG00000082701.101179.95210938.159100.330702.284901.42750HepG2SERBP1
GSK3BENSG00000082701.102718.218901957.831500.473303.093102.37980HepG2SF3A3
GSK3BENSG00000082701.101699.282901361.293600.319804.152703.45180HepG2SF3B4
GSK3BENSG00000082701.101513.364502052.30330-0.439502.309101.59780HepG2SNRNP70
GSK3BENSG00000082701.103636.339201970.640300.8837040.1276038.80010HepG2SRFBP1
GSK3BENSG00000082701.102555.654501420.934100.8468029.6321028.44860HepG2SRSF1
GSK3BENSG00000082701.103168.337901984.463200.674904.451003.15100HepG2SRSF3
GSK3BENSG00000082701.102141.587501489.442300.523803.921203.19830HepG2SRSF5
GSK3BENSG00000082701.101360.514001156.038600.234902.512901.98120HepG2SRSF9
GSK3BENSG00000082701.102178.510701711.037600.348405.897805.20310HepG2SSB
GSK3BENSG00000082701.102075.504901694.002800.292902.920102.28480HepG2SUCLG1
GSK3BENSG00000082701.10971.260501195.41600-0.299602.325501.45850HepG2SUPT6H
GSK3BENSG00000082701.101827.104701302.961800.487703.426002.68300HepG2SUPV3L1
GSK3BENSG00000082701.101422.517801173.549400.277502.372101.59640HepG2TIA1
GSK3BENSG00000082701.10825.529801447.89530-0.8106020.5876019.23000HepG2TRIM56
GSK3BENSG00000082701.103017.439301514.319800.9946019.5176017.79750HepG2U2AF1
GSK3BENSG00000082701.102561.878501676.326700.6118016.7516015.46100HepG2UTP3
GSK3BENSG00000082701.10756.574901282.61820-0.761503.335002.54380HepG2XRN2
GSK3BENSG00000082701.102058.280201331.364200.6284017.7366016.29470HepG2ZC3H8
GSK3BENSG00000082701.101684.781101082.675000.637908.001107.09010K562AQR
GSK3BENSG00000082701.10967.45030639.945600.596104.608703.23240K562EIF3D
GSK3BENSG00000082701.10824.18330669.880500.299002.372401.80400K562FXR1
GSK3BENSG00000082701.101011.90270704.590400.522103.428802.62510K562G3BP2
GSK3BENSG00000082701.10410.73030599.23950-0.544903.637501.96930K562GEMIN5
GSK3BENSG00000082701.101078.010501466.06840-0.443602.019101.33430K562HSPD1
GSK3BENSG00000082701.10683.35820882.12890-0.368302.948501.99290K562MATR3
GSK3BENSG00000082701.10831.90680643.213000.371002.979801.70610K562NCBP2
GSK3BENSG00000082701.10935.760501120.54800-0.260003.012302.23750K562NSUN2
GSK3BENSG00000082701.102209.731001566.907500.495905.824004.64460K562PUF60
GSK3BENSG00000082701.101362.311201099.668200.308902.177501.68420K562PUS1
GSK3BENSG00000082701.10399.71940592.45040-0.567703.622702.68590K562RPS19
GSK3BENSG00000082701.10411.10640594.68640-0.532602.792501.45990K562RRP9
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for GSK3B is not available on ENCORE database.





11 RBPs can affect the splicing of GSK3B :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEGSK3BENSG00000082701.10chr3-1195854361195854751195822651195824521195952591195953550.565000.000000.00000includedHepG2U2AF1
SEGSK3BENSG00000082701.10chr3-119562101119562200119545532119545702119582265119582452-0.099000.001340.02627excludedHepG2U2AF1
SEGSK3BENSG00000082701.10chr3-1195854361195854751195822651195824521195952591195953550.130000.001590.03921includedHepG2HNRNPK
SEGSK3BENSG00000082701.10chr3-1195854361195854751195822651195824521195952591195953550.321000.000000.00000includedHepG2U2AF2
SEGSK3BENSG00000082701.10chr3-119595259119595355119582265119582452119624601119624722-0.113000.000490.00667excludedK562AQR
SEGSK3BENSG00000082701.10chr3-119562101119562200119545532119545702119582265119582452-0.242000.000000.00000excludedK562AQR
SEGSK3BENSG00000082701.10chr3-1195854361195854751195822651195824521195952591195953550.357000.000000.00000includedK562U2AF1
SEGSK3BENSG00000082701.10chr3-119562101119562200119540169119545702119582265119582452-0.151000.006520.09917excludedK562U2AF1
SEGSK3BENSG00000082701.10chr3-119585436119585475119582265119582452119595259119595355-0.097000.000060.00250excludedHepG2SRSF1
SEGSK3BENSG00000082701.10chr3-1195854361195854751195822651195824521195952591195953550.235000.000000.00017includedK562U2AF2
SEGSK3BENSG00000082701.10chr3-1195854361195854751195822651195824521195952591195953550.154000.000010.00064includedHepG2HNRNPC
A3SSGSK3BENSG00000082701.10chr3-1195952591195953871195952591195953551196246011196246990.066000.001700.01115longerK562AQR
A3SSGSK3BENSG00000082701.10chr3-1195455321195457411195455321195457021195621011195622000.055000.004470.02619longerK562AQR
MXEGSK3BENSG00000082701.10chr3-1195952591195953551196246011196246991195822651195824521196315501196316570.283000.000000.000001includedHepG2U2AF1
MXEGSK3BENSG00000082701.10chr3-119595259119595355119624601119624699119585436119585475119631550119631657-0.349000.000000.000002includedHepG2U2AF1
MXEGSK3BENSG00000082701.10chr3-119585436119585475119592418119592523119582265119582452119595259119595355-0.061000.000200.005862includedHepG2U2AF1
MXEGSK3BENSG00000082701.10chr3-119585436119585475119595259119595355119582265119582452119624601119624699-0.401000.000000.000002includedHepG2U2AF1
MXEGSK3BENSG00000082701.10chr3-1195952591195953551196246011196246991195822651195824521196315501196316570.159000.000000.000191includedHepG2FTO
MXEGSK3BENSG00000082701.10chr3-1195854361195854751195952591195953551195822651195824521196246011196246990.074000.000190.006211includedHepG2SRSF1
MXEGSK3BENSG00000082701.10chr3-119595259119595355119624601119624699119582265119582452119631550119631657-0.264000.000080.006672includedK562FXR1
MXEGSK3BENSG00000082701.10chr3-1195952591195953551196246011196247221195822651195824521196315501196316570.147000.000190.002141includedK562AQR
MXEGSK3BENSG00000082701.10chr3-1195952591195953551196246011196247461195822651195824521196315501196316570.137000.000500.004991includedK562AQR
MXEGSK3BENSG00000082701.10chr3-119582265119582452119595259119595355119562101119562200119624601119624699-0.194000.000130.014792includedHepG2NOLC1
MXEGSK3BENSG00000082701.10chr3-119595259119595355119624601119624699119582265119582452119631550119631657-0.154000.016730.081142includedHepG2U2AF2
MXEGSK3BENSG00000082701.10chr3-119595259119595355119624601119624699119582265119582452119631550119631657-0.205000.001400.023792includedHepG2HNRNPUL1
MXEGSK3BENSG00000082701.10chr3-119595259119595355119624601119624699119582265119582452119631550119631657-0.239000.000340.043242includedK562EWSR1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for GSK3B is not avaliable on ENCORE database.