GSK3A antibody: MA3-038


Antibody Info
Product_IDMA3-038
Lot_IDRF227934
Host_organismmouse
SourceThermo Fisher
Antibody_DCC_IDENCAB957NOP
Target Info
GeneNameGSK3A
Phosphate
Target_nameGSK3A-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 GSK3alpha-HEPG2-CRISPR-MA3-038.png<br>Caption: Western blot following CRISPR against GSK3A in HepG2 whole cell lysate using GSK3A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3A. GSK3A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against GSK3A in HepG2 whole cell lysate using GSK3A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3A. GSK3A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV14-41

Status: Released

Lab: Graveley Lab

GSK3beta-HEPG2-CRISPR-MA3-038.png<br>Caption: Western blot following CRISPR against GSK3B in HepG2 whole cell lysate using GSK3B specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3B. GSK3B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against GSK3B in HepG2 whole cell lysate using GSK3B specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3B. GSK3B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV16-31

Status: Released

Lab: Graveley Lab

K562 ThermoFisher_MA3-038_RF227934_GSK3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the GSK3 specific antibody, MA3-038. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GSK3-specific antibody, MA3-038. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the GSK3 specific antibody, MA3-038. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GSK3-specific antibody, MA3-038. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

 GSK3alpha-K562-CRISPR-MA3-038.png<br>Caption: Western blot following CRISPR against GSK3A in K562 whole cell lysate using GSK3A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3A. GSK3A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against GSK3A in K562 whole cell lysate using GSK3A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3A. GSK3A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGKcLV13-41

Status: Released

Lab: Graveley Lab

GSK3beta-K562-CRISPR-MA3-038.png<br>Caption: Western blot following CRISPR against GSK3B in K562 whole cell lysate using GSK3B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3B. GSK3B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against GSK3B in K562 whole cell lysate using GSK3B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSK3B. GSK3B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGKcLV15-37

Status: Released

Lab: Graveley Lab