APOL2

General Information

Gene Name       : APOL2
Gene ID             : ENSG00000128335.13
Essential Gene : No

Functions

Localization

Domains (AlphaFold: APOL2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878APOL2ENSG00000128335.1314.1016.9215.51ENCSR000AEF
HepG2APOL2ENSG00000128335.1325.8920.5923.24ENCSR985KAT
K562APOL2ENSG00000128335.136.786.016.39ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  

No Antibody Primary Validation image or Secondary image available



  KD RNA-Seq  

No KD RNA-Seq experiment available



  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela








RNA Targets

  • eCLIP assay for APOL2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • APOL2 RNA is bound by 23 proteins.  







eCLIP assay for APOL2 is not avaliable on ENCORE database.





23 proteins are bound to APOL2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr223662946336629518-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.264347.20962HepG2PPIG
chr223662277736622883-3'-UTR,intron-Distal3.181845.97918HepG2UPF1
chr223662331436623365-3'-UTR,intron-Distal,intron-Proximal3.368343.72765HepG2UPF1
chr223662289336623030-3'-UTR,intron-Distal3.6662510.08726HepG2UPF1
chr223662332236623365-3'-UTR,intron-Distal,intron-Proximal3.054653.01653HepG2LSM11
chr223662944636629463-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.331343.95327HepG2PPIG
chr223662916336629214-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal4.759604.28193HepG2AQR
chr223662279736622859-3'-UTR,intron-Distal3.670043.29239HepG2CSTF2
chr223662926836629288-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal5.267185.27053HepG2PPIG
chr223663551436635599-5'-UTR,CDS,intron-Distal,intron-Proximal4.149253.89619HepG2SRSF1
chr223662736336627542-CDS,intron-Distal,intron-Proximal4.396918.28249HepG2AQR
chr223662428836624341-CDS,intron-Distal4.401816.32636HepG2U2AF2
chr223662743836627484-CDS,intron-Distal3.585065.67970HepG2PABPN1
chr223662738036627459-CDS,intron-Distal,intron-Proximal3.705189.47710HepG2PPIG
chr223662395936623986-CDS,intron-Distal,intron-Proximal3.400317.77469HepG2PABPN1
chr223662352836623593-CDS,intron-Distal3.611136.99093HepG2PABPN1
chr223662374936623799-CDS,intron-Distal3.186774.21526HepG2PABPN1
chr223662915836629203-CDS,intron-Distal,intron-Proximal5.340545.35117HepG2PRPF8
chr223662379936623848-CDS,intron-Distal3.670028.24193HepG2PABPN1
chr223662398636624029-CDS,intron-Distal,intron-Proximal3.340107.54003HepG2PABPN1
chr223662391436623959-CDS,intron-Distal3.496587.20787HepG2PABPN1
chr223663390736633999-intron-Distal4.553213.85108HepG2GTF2F1
chr223663091636630961-intron-Distal3.6458210.93678HepG2SUGP2
chr223663180036631882-intron-Distal3.8663847.89767HepG2HNRNPL
chr223663221536632261-intron-Distal4.595035.60990HepG2KHSRP
chr223663103736631057-intron-Distal3.991314.61322HepG2MATR3
chr223663204336632099-intron-Distal3.209314.30158HepG2U2AF2
chr223663126536631386-intron-Distal3.6390338.58269HepG2SUGP2
chr223663056936630621-intron-Distal3.009603.66814K562U2AF2
chr223663170736631782-intron-Distal3.943847.92999HepG2PTBP1
chr223662930236629340-intron-Distal,intron-Proximal4.100693.98315HepG2SF3B4
chr223663172736631801-intron-Distal4.192644.19392HepG2GTF2F1
chr223663096136631010-intron-Distal3.129009.18352HepG2SUGP2
chr223663138636631460-intron-Distal3.1931416.67648HepG2SUGP2
chr223663393736633997-intron-Distal3.708729.42296HepG2PRPF4
chr223662910536629174-intron-Distal,intron-Proximal5.286797.65545HepG2RBM22
chr223662523536625340-intron-Distal3.297454.14863HepG2SAFB
chr223663162336631673-intron-Distal4.7997810.55454HepG2PTBP1
chr223662913236629184-intron-Distal,intron-Proximal4.678743.61881HepG2TIAL1
chr223663234136632425-intron-Distal5.197337.43163HepG2QKI
chr223663056436630622-intron-Distal6.3528913.47728HepG2U2AF2
chr223663543236635487-intron-Distal,intron-Proximal4.168423.45994HepG2NCBP2
chr223663244636632558-intron-Distal3.3885512.42434HepG2KHSRP
chr223663132236631380-intron-Distal4.1967614.35124HepG2MATR3
chr223663388836634012-intron-Distal3.161043.02806HepG2SAFB
chr223663167336631707-intron-Distal5.135478.82480HepG2PTBP1
chr223663074236630787-intron-Distal3.230683.78824HepG2KHSRP
chr223663202536632098-intron-Distal4.8056913.97042HepG2PTBP1
chr223663170536631791-intron-Distal3.4823011.47988HepG2AGGF1
chr223663134036631440-intron-Distal3.670189.12237HepG2PTBP1
chr223663087336630916-intron-Distal3.547748.63136HepG2SUGP2
chr223663234936632412-intron-Distal3.312134.37446HepG2MATR3
chr223663177136631800-intron-Distal4.3793835.10182HepG2HNRNPL
chr223663116036631222-intron-Distal4.134557.91367HepG2MATR3
chr223663221936632341-intron-Distal4.2703511.28513HepG2QKI
chr223663175536631771-intron-Distal4.9858542.70834HepG2HNRNPL
chr223663171536631755-intron-Distal4.9592537.04556HepG2HNRNPL
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for APOL2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for APOL2 is not available on ENCORE database.

  • 141 RBPs can affect the levels of APOL2.  








The Knockdown/knockout RNA-Seq assay for APOL2 is not available on ENCORE database.





141 RBPs can affect the gene expression of APOL2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
APOL2ENSG00000128335.91201.923701661.77860-0.467404.065602.93830HepG2ABCF1
APOL2ENSG00000128335.91615.583202923.65660-0.8557010.854109.86780HepG2AKAP1
APOL2ENSG00000128335.91868.540001408.581200.407606.397005.42150HepG2AUH
APOL2ENSG00000128335.92195.39040920.950101.2532023.2837021.09920HepG2BCLAF1
APOL2ENSG00000128335.92340.387501244.774900.9108018.2244015.43000HepG2BUD13
APOL2ENSG00000128335.91934.912301228.894300.6548011.301909.91330HepG2CALR
APOL2ENSG00000128335.91155.842301626.45640-0.492803.339102.25950HepG2CCAR1
APOL2ENSG00000128335.94393.768002298.249400.9348017.1381015.41840HepG2CIRBP
APOL2ENSG00000128335.91239.069501545.62390-0.318904.709803.88590HepG2CKAP4
APOL2ENSG00000128335.9711.862602692.11200-1.9191030.8733028.44850HepG2CNOT7
APOL2ENSG00000128335.92558.895401368.174500.9032018.4786015.92490HepG2CPSF7
APOL2ENSG00000128335.91482.65220975.714500.6035010.345408.44390HepG2YBX3
APOL2ENSG00000128335.9492.56470654.73540-0.410602.352401.35400HepG2CSTF2
APOL2ENSG00000128335.93564.412801933.465200.882408.403407.39870HepG2CELF1
APOL2ENSG00000128335.91041.528201374.59370-0.400303.638502.86940HepG2DDX21
APOL2ENSG00000128335.91932.208002800.49310-0.535405.412604.56350HepG2DDX3X
APOL2ENSG00000128335.93997.800602961.175900.432905.025703.30800HepG2DDX47
APOL2ENSG00000128335.92029.683301041.948800.9619011.166109.73770HepG2DDX55
APOL2ENSG00000128335.91314.09150891.106300.560302.468401.54570HepG2DDX59
APOL2ENSG00000128335.91881.558101270.860200.566005.589804.20090HepG2DDX5
APOL2ENSG00000128335.95757.916302406.031701.258809.994108.89990HepG2DDX6
APOL2ENSG00000128335.91961.87640924.032501.0861023.5155021.17870HepG2DHX30
APOL2ENSG00000128335.91979.043001479.188300.419907.054806.06920HepG2DKC1
APOL2ENSG00000128335.91164.77890958.024700.281802.552801.87270HepG2DNAJC21
APOL2ENSG00000128335.9686.475101164.81470-0.762808.508006.99100HepG2EEF2
APOL2ENSG00000128335.93712.577402312.629100.682808.894207.86930HepG2EIF2S1
APOL2ENSG00000128335.91989.363102555.55880-0.361302.444401.84180HepG2EIF4A3
APOL2ENSG00000128335.91301.303102488.51610-0.9353010.153608.87840HepG2EIF4B
APOL2ENSG00000128335.92785.148301964.619900.503402.699301.89380HepG2EIF4G2
APOL2ENSG00000128335.91124.234101439.30530-0.356402.952301.82620HepG2ESF1
APOL2ENSG00000128335.9881.227801617.30200-0.8760023.7601022.29710HepG2ETF1
APOL2ENSG00000128335.91942.471201510.694900.362602.523501.72510HepG2EWSR1
APOL2ENSG00000128335.91659.429301309.806700.341202.955301.77690HepG2FASTKD2
APOL2ENSG00000128335.9985.698401677.92730-0.767508.350607.09430HepG2FIP1L1
APOL2ENSG00000128335.91377.25630867.316900.667106.904205.01790HepG2FKBP4
APOL2ENSG00000128335.91291.68490786.151900.716302.822401.79740HepG2FTO
APOL2ENSG00000128335.91115.032101524.59860-0.451302.604501.52060HepG2FUS
APOL2ENSG00000128335.91982.043702578.96060-0.379803.065702.33760HepG2FXR1
APOL2ENSG00000128335.9947.316202392.09640-1.3364025.8498023.88340HepG2G3BP1
APOL2ENSG00000128335.9500.04800841.89150-0.751607.742306.27680HepG2GEMIN5
APOL2ENSG00000128335.91796.460201286.312000.481804.146702.89760HepG2GNB2L1
APOL2ENSG00000128335.91156.842002492.26950-1.1073012.8675010.53960HepG2GRSF1
APOL2ENSG00000128335.92737.715601910.426800.519006.002004.71990HepG2HNRNPA2B1
APOL2ENSG00000128335.91569.298102223.71880-0.502903.757602.11610HepG2HNRNPAB
APOL2ENSG00000128335.9724.810401075.86130-0.569805.678404.71250HepG2HNRNPC
APOL2ENSG00000128335.91444.040702331.95560-0.691405.980303.77490HepG2HNRNPD
APOL2ENSG00000128335.91373.85470874.979000.6508012.0515010.69860HepG2HNRNPF
APOL2ENSG00000128335.93691.576202422.839300.607405.213804.27820HepG2HNRNPK
APOL2ENSG00000128335.92555.072201893.242200.432409.350108.18120HepG2HNRNPL
APOL2ENSG00000128335.9863.022701157.50320-0.423504.146903.00270HepG2HNRNPM
APOL2ENSG00000128335.9547.147401068.74650-0.9659023.1970021.57380HepG2HNRNPU
APOL2ENSG00000128335.91599.653603110.09880-0.9592012.258009.77790HepG2HSPD1
APOL2ENSG00000128335.91453.46330861.594700.7543012.2003010.78810HepG2IGF2BP1
APOL2ENSG00000128335.9961.215202282.16480-1.247506.640705.15560HepG2IGF2BP3
APOL2ENSG00000128335.91424.224201091.684700.383506.454205.38200HepG2ILF2
APOL2ENSG00000128335.91859.410202624.65910-0.497303.219501.77790HepG2ILF3
APOL2ENSG00000128335.91578.430602566.04530-0.701106.933504.95240HepG2KHDRBS1
APOL2ENSG00000128335.93832.875202445.769600.648005.924904.40680HepG2KHSRP
APOL2ENSG00000128335.91714.129001413.252900.278404.036402.81100HepG2KIF1C
APOL2ENSG00000128335.91691.711103107.48930-0.877305.969104.12080HepG2KRR1
APOL2ENSG00000128335.9806.236701717.05710-1.0907013.7356011.91870HepG2LARP4
APOL2ENSG00000128335.9923.770501260.25480-0.448104.274902.85660HepG2LARP7
APOL2ENSG00000128335.9773.662701323.18750-0.7742010.573308.34170HepG2LIN28B
APOL2ENSG00000128335.91199.297202579.86320-1.1051019.6848018.01720HepG2MAGOH
APOL2ENSG00000128335.9891.032802594.21960-1.5418032.0260030.06510HepG2MATR3
APOL2ENSG00000128335.91462.23720865.932000.755802.924601.92510HepG2MTPAP
APOL2ENSG00000128335.91724.259001140.135900.596703.118001.82500HepG2NAA15
APOL2ENSG00000128335.91693.880902655.26070-0.648507.366906.35770HepG2NCBP2
APOL2ENSG00000128335.92348.75170752.697401.6417045.8598043.46960HepG2NSUN2
APOL2ENSG00000128335.93577.560402460.854500.539702.960902.04250HepG2PABPC1
APOL2ENSG00000128335.9593.233101057.84350-0.834508.166806.83470HepG2PARN
APOL2ENSG00000128335.91865.077202392.85740-0.359503.464302.80170HepG2PCBP1
APOL2ENSG00000128335.9549.72750781.95390-0.508404.951103.32700HepG2PKM
APOL2ENSG00000128335.91329.74330953.231900.480106.698405.59060HepG2PPIL4
APOL2ENSG00000128335.91106.89380836.887300.403304.999003.99160HepG2PRPF4
APOL2ENSG00000128335.9558.65080804.00270-0.525303.371902.48810HepG2PRPF8
APOL2ENSG00000128335.91333.998102434.98350-0.8682011.7228010.75020HepG2PTBP1
APOL2ENSG00000128335.91329.257101967.59960-0.565805.933705.10520HepG2PUF60
APOL2ENSG00000128335.9938.752302088.83330-1.1539020.1842018.87990HepG2PUM1
APOL2ENSG00000128335.91320.918402087.22080-0.660008.292107.15840HepG2PUM2
APOL2ENSG00000128335.91085.258501574.81490-0.5371011.074709.98260HepG2PUS1
APOL2ENSG00000128335.92058.046402636.80270-0.357502.361701.77680HepG2QKI
APOL2ENSG00000128335.92860.373901832.636900.6422013.0795011.42510HepG2RBM15
APOL2ENSG00000128335.9929.758301351.75220-0.539908.171707.39060HepG2RBM22
APOL2ENSG00000128335.93204.599802393.198100.421105.609604.68130HepG2RBM25
APOL2ENSG00000128335.9731.250101519.67130-1.0553017.2276014.80240HepG2RBM27
APOL2ENSG00000128335.91264.619702600.39540-1.0400012.114709.21130HepG2RBM47
APOL2ENSG00000128335.91485.584202709.76850-0.8671016.7735015.65760HepG2NELFE
APOL2ENSG00000128335.91342.396802524.02480-0.9109016.6123015.30890HepG2RECQL
APOL2ENSG00000128335.91248.65420942.713900.405402.970502.26610HepG2RPLP0
APOL2ENSG00000128335.91903.981901368.183300.476702.720601.78430HepG2RPS10
APOL2ENSG00000128335.9408.32120920.08490-1.1721020.5639019.12850HepG2RPS19
APOL2ENSG00000128335.9672.826901401.52500-1.0587017.5212015.63240HepG2RPS2
APOL2ENSG00000128335.91571.541002704.09710-0.783007.713806.39790HepG2RPS3A
APOL2ENSG00000128335.9929.252001430.66870-0.622506.980105.28580HepG2RPS5
APOL2ENSG00000128335.9990.149401515.36300-0.613903.964902.12330HepG2RTF1
APOL2ENSG00000128335.91245.25550951.750700.387705.557904.69820HepG2SAFB2
APOL2ENSG00000128335.91316.01180923.421400.511004.819103.59270HepG2SERBP1
APOL2ENSG00000128335.91111.551302073.68050-0.899609.321308.02470HepG2SF3A3
APOL2ENSG00000128335.91616.74320840.040400.9445012.3467010.95660HepG2SF3B1
APOL2ENSG00000128335.92489.204401886.907600.399605.935104.96950HepG2SLBP
APOL2ENSG00000128335.91066.474101420.51270-0.413602.346001.47210HepG2SMNDC1
APOL2ENSG00000128335.93955.276201526.731401.3732089.4488087.62790HepG2SRFBP1
APOL2ENSG00000128335.9817.355102645.94480-1.6947044.3850042.99180HepG2SRSF1
APOL2ENSG00000128335.91232.784701942.88510-0.656303.108402.45020HepG2SRSF5
APOL2ENSG00000128335.91258.06410859.671900.549208.465007.36230HepG2SRSF7
APOL2ENSG00000128335.95241.634102152.719001.2838075.2500073.18130HepG2SRSF9
APOL2ENSG00000128335.91158.681701325.52420-0.194101.778101.34470HepG2SSB
APOL2ENSG00000128335.92274.859603098.31240-0.445703.643902.18900HepG2SSRP1
APOL2ENSG00000128335.91631.131002352.54400-0.528304.014002.66620HepG2STIP1
APOL2ENSG00000128335.92688.738501301.483401.0467011.253308.97190HepG2SUB1
APOL2ENSG00000128335.9941.401202209.45250-1.2308020.1843018.75290HepG2SUCLG1
APOL2ENSG00000128335.91874.575101132.751800.7266011.784309.97590HepG2SUPT6H
APOL2ENSG00000128335.91272.329401037.308700.294501.914701.33760HepG2SUPV3L1
APOL2ENSG00000128335.91187.915502378.60670-1.0017016.8025015.58750HepG2TAF15
APOL2ENSG00000128335.9875.191303043.26010-1.7979017.8377015.46370HepG2TBRG4
APOL2ENSG00000128335.91232.58110695.311200.8259010.064908.39890HepG2TFIP11
APOL2ENSG00000128335.9555.368401038.38940-0.9028015.3696014.36080HepG2TIAL1
APOL2ENSG00000128335.91371.276801047.776500.388102.518801.97550HepG2TRIM56
APOL2ENSG00000128335.9493.67210751.34600-0.605904.273902.34210HepG2TROVE2
APOL2ENSG00000128335.91388.809501922.48920-0.469103.398102.41960HepG2TUFM
APOL2ENSG00000128335.91804.09240950.028000.925109.827507.87210HepG2UPF2
APOL2ENSG00000128335.9755.466601531.63530-1.0196010.622408.69820HepG2XRCC6
APOL2ENSG00000128335.91718.055801021.114700.750509.759808.41610HepG2XRN2
APOL2ENSG00000128335.94125.935702022.589401.0284067.4324065.67400HepG2ZNF622
APOL2ENSG00000128335.9946.153001391.54790-0.556503.900302.33700HepG2ZRANB2
APOL2ENSG00000128335.9855.89680603.115800.504904.155803.46510K562AQR
APOL2ENSG00000128335.9567.17490268.427501.079208.749907.14910K562BOP1
APOL2ENSG00000128335.9375.03460285.411800.393902.053601.36840K562CPSF6
APOL2ENSG00000128335.9559.26930313.588500.834607.150304.57410K562DDX51
APOL2ENSG00000128335.9438.11100300.489900.543902.694101.43320K562EEF2
APOL2ENSG00000128335.9463.65710290.325400.675304.785003.56970K562EFTUD2
APOL2ENSG00000128335.9471.62240327.821800.524604.519802.95030K562EIF2S1
APOL2ENSG00000128335.9563.37130310.838900.857806.785505.07280K562EIF3D
APOL2ENSG00000128335.9481.44160311.795500.626702.448401.67620K562EIF4A3
APOL2ENSG00000128335.9414.25120290.406900.512302.808701.94850K562FMR1
APOL2ENSG00000128335.9397.17070246.149200.690104.492403.30130K562FTO
APOL2ENSG00000128335.9465.30940324.255400.521004.133503.41570K562FXR1
APOL2ENSG00000128335.9123.17740241.75810-0.972805.227904.18560K562G3BP2
APOL2ENSG00000128335.9514.93210272.495000.918108.908807.47530K562GPKOW
APOL2ENSG00000128335.9891.16680523.579900.767208.672207.31370K562GRSF1
APOL2ENSG00000128335.9554.92750237.829101.2223015.5156014.18510K562HNRNPC
APOL2ENSG00000128335.9351.82650236.797300.571102.904801.94810K562HNRNPM
APOL2ENSG00000128335.9494.82530366.977800.431103.391402.50060K562MTPAP
APOL2ENSG00000128335.9430.90440311.336500.468803.043601.75530K562NCBP2
APOL2ENSG00000128335.9525.81840407.708500.366902.986302.21470K562NSUN2
APOL2ENSG00000128335.9474.89140326.816900.539003.579202.62620K562PPIG
APOL2ENSG00000128335.9454.66220282.520300.686304.567503.60370K562PRPF8
APOL2ENSG00000128335.9684.04910313.005901.1278017.8841016.26960K562PTBP1
APOL2ENSG00000128335.9996.23600637.434400.644105.088503.98470K562PUF60
APOL2ENSG00000128335.9516.93690390.595600.404202.010101.53290K562PUS1
APOL2ENSG00000128335.9409.21280194.519601.0728010.896308.90930K562RCC2
APOL2ENSG00000128335.9476.91060259.163300.879806.557605.02420K562SF3B1
APOL2ENSG00000128335.9427.35330310.527200.460602.623201.93280K562TARDBP
APOL2ENSG00000128335.9561.75660431.762000.379602.780701.50550K562TROVE2
APOL2ENSG00000128335.9533.85180432.304900.304302.024501.40420K562UPF1
APOL2ENSG00000128335.9559.45970369.904900.596805.254604.02800K562WDR3
APOL2ENSG00000128335.9525.85920384.690800.450904.178403.15430K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for APOL2 is not available on ENCORE database.





5 RBPs can affect the splicing of APOL2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEAPOL2ENSG00000128335.9chr22-3663028136630381366294473662950136635486366356070.059000.001370.02676includedHepG2U2AF1
SEAPOL2ENSG00000128335.9chr22-3663028136630381366294473662950136635486366356070.175000.000520.00803includedHepG2U2AF2
SEAPOL2ENSG00000128335.9chr22-3663468036634834366329783663340936635486366356070.077000.002850.09137includedHepG2MATR3
SEAPOL2ENSG00000128335.9chr22-3663264136632858366294473662950136635486366356070.087000.000380.01411includedHepG2SF3A3
SEAPOL2ENSG00000128335.9chr22-3663468036634834366294473662950136635486366356070.068000.003350.06779includedK562RBM15

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for APOL2 is not avaliable on ENCORE database.