ERN1

General Information

Gene Name       : ERN1
Gene ID             : ENSG00000178607.15
Essential Gene : No

Functions

Localization

Domains (AlphaFold: ERN1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ERN1ENSG00000178607.154.094.594.34ENCSR000AEF
HepG2ERN1ENSG00000178607.154.744.904.82ENCSR985KAT
K562ERN1ENSG00000178607.155.914.425.17ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ERN1-BGKcLV17-K562ENCSR026FEMERN1K562ReleasedENCSR516TLX   CRISPRKD% = 92.9,96.8
knockdown followed by RNA-seq ERN1-BGHcLV18-HepG2ENCSR005UDTERN1HepG2ReleasedENCSR670KAS   CRISPRKD% = 95.2,98.4






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ERN1ENCAB311UNOMA5-14991TL2693035Released, ENCODE4BGHcLV18-17, Released, ENCODE4BGKcLV17-17, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ERN1K562CRISPRENCSR026FEMENCSR516TLXERN1-BGKcLV17-17,ERN1-BGKcLV17-18NT-BGKcLV17-1,NT-BGKcLV17-292.9,96.8ReleasedENCODE4
ERN1HepG2CRISPRENCSR005UDTENCSR670KASERN1-BGHcLV18-17,ERN1-BGHcLV18-18NT-BGHcLV18-1,NT-BGHcLV18-295.2,98.4ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ERN1Product_ID: MA5-14991
Lot_ID: TL2693035
Source: Thermo Fisher
Target Name: ERN1-human		 ERN1-HEPG2-CRISPR-MA5-14991.png<br>Caption: Western blot following CRISPR against ERN1 in HepG2 whole cell lysate using ERN1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ERN1. ERN1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ThermoFisher_MA5-14991_TL2693035_ERN1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ERN1 specific antibody, MA5-14991. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the ERN1-specific antibody, MA5-14991. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ERN1-K562-CRISPR-MA5-14991.png<br>Caption: Western blot following CRISPR against ERN1 in K562 whole cell lysate using ERN1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ERN1. ERN1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ERN1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • ERN1 RNA is bound by 10 proteins.  







eCLIP assay for ERN1 is not avaliable on ENCORE database.





10 proteins are bound to ERN1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr176212092762121035-3'-UTR,intron-Distal3.711666.04024K562UPF1
chr176211914162119188-3'-UTR,intron-Distal3.243825.82512HepG2SUGP2
chr176211741762117458-3'-UTR,intron-Distal3.332995.59399HepG2SUGP2
chr176211709462117156-3'-UTR,intron-Distal4.000777.87505K562UPF1
chr176211876262118832-3'-UTR,intron-Distal4.258808.81208HepG2SUGP2
chr176211781562117875-3'-UTR,intron-Distal3.090526.11961HepG2SUGP2
chr176211745162117510-3'-UTR,intron-Distal3.838286.76837K562UPF1
chr176211745862117507-3'-UTR,intron-Distal3.6977811.65979HepG2SUGP2
chr176212106962121271-3'-UTR,intron-Distal5.158216.52213HepG2UPF1
chr176211746862117504-3'-UTR,intron-Distal5.345386.97910HepG2PCBP2
chr176211883762118959-3'-UTR,intron-Distal4.1181815.42976HepG2SUGP2
chr176212049562120601-3'-UTR,intron-Distal4.275096.74636K562UPF1
chr176212078562120885-3'-UTR,intron-Distal5.5539111.21630K562UPF1
chr176220732662207479-5'-UTR,CDS,intron-Distal4.388795.48791HepG2DDX3X
chr176213212462132203-CDS,intron-Distal,intron-Proximal3.566613.32861HepG2U2AF2
chr176214129362141358-CDS,intron-Distal,intron-Proximal5.253247.59497HepG2PRPF8
chr176212655362126611-CDS,intron-Distal,intron-Proximal4.078503.05889HepG2SF3B4
chr176212518062125252-CDS,intron-Distal,intron-Proximal4.160047.87148HepG2PRPF8
chr176212268762122794-CDS,intron-Distal,intron-Proximal3.042163.73413HepG2PPIG
chr176215544062155540-intron-Distal4.611155.29097HepG2LIN28B
chr176216853462168619-intron-Distal4.737194.66899HepG2HNRNPM
chr176215544062155536-intron-Distal4.878234.96063K562LIN28B
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for ERN1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ERN1 is not available on ENCORE database.

  • 126 RBPs can affect the levels of ERN1.  








The Knockdown/knockout RNA-Seq assay for ERN1 is not available on ENCORE database.





126 RBPs can affect the gene expression of ERN1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
ERN1ENSG00000178607.11407.79330554.49500-0.443302.256401.40070HepG2ABCF1
ERN1ENSG00000178607.11365.23120486.08640-0.412403.300402.64620HepG2ACO1
ERN1ENSG00000178607.11610.03270488.394600.320702.391401.67270HepG2AKAP8L
ERN1ENSG00000178607.11826.45670500.495700.723507.792405.56110HepG2ATP5C1
ERN1ENSG00000178607.11859.43310700.177100.295602.489301.83190HepG2AUH
ERN1ENSG00000178607.11513.94730254.766301.012307.835806.41800HepG2BCLAF1
ERN1ENSG00000178607.11776.59530361.709701.1022015.3455014.09440HepG2BOP1
ERN1ENSG00000178607.11650.72580371.086800.810209.246907.96000HepG2CALR
ERN1ENSG00000178607.11711.462501090.12460-0.615602.666601.91700HepG2CNOT7
ERN1ENSG00000178607.11485.13930332.068200.546803.448702.68870HepG2CPSF6
ERN1ENSG00000178607.111197.92050456.721101.3910030.2415027.23000HepG2CPSF7
ERN1ENSG00000178607.11491.39220376.268500.385002.286001.36240HepG2YBX3
ERN1ENSG00000178607.11610.07550467.782200.383103.037902.36990HepG2CELF1
ERN1ENSG00000178607.111072.79720502.978701.0927026.0510024.21330HepG2DAZAP1
ERN1ENSG00000178607.11640.44060376.789300.765209.354207.42690HepG2DDX1
ERN1ENSG00000178607.11331.67240218.356800.603002.738901.35650HepG2DDX28
ERN1ENSG00000178607.111881.704201308.088200.524505.523703.73440HepG2DDX47
ERN1ENSG00000178607.11489.93590288.334400.764704.328403.38390HepG2DDX55
ERN1ENSG00000178607.11714.76540377.088200.922505.689004.30320HepG2DDX59
ERN1ENSG00000178607.111800.70110944.232200.9312011.5604010.40230HepG2DDX6
ERN1ENSG00000178607.11697.84090356.347400.969509.088507.41810HepG2DHX30
ERN1ENSG00000178607.11655.97490369.461500.8281011.4225010.07210HepG2DNAJC21
ERN1ENSG00000178607.11574.57730298.176900.946207.701805.89810HepG2AGO1
ERN1ENSG00000178607.11636.41360420.693000.597105.565003.94690HepG2EIF2S2
ERN1ENSG00000178607.11596.24820319.802300.898608.175306.69570HepG2EIF3D
ERN1ENSG00000178607.11753.723501003.21720-0.412502.447301.84430HepG2EIF4A3
ERN1ENSG00000178607.111103.94690496.221101.1535029.4500027.35110HepG2EIF4G1
ERN1ENSG00000178607.11744.02100475.347700.646304.581403.55050HepG2EIF4G2
ERN1ENSG00000178607.111253.64280803.891600.6410011.8004010.70070HepG2ETF1
ERN1ENSG00000178607.11389.58860285.842300.446602.730101.57010HepG2FAM120A
ERN1ENSG00000178607.11530.46830308.607400.781407.329905.63010HepG2FASTKD2
ERN1ENSG00000178607.11692.84130332.639501.058504.946103.59150HepG2FTO
ERN1ENSG00000178607.11974.04220604.068900.689208.176606.85960HepG2FUBP3
ERN1ENSG00000178607.11442.554701012.12370-1.1935017.2514015.69000HepG2FXR1
ERN1ENSG00000178607.11697.22260938.93550-0.429402.769502.10400HepG2G3BP1
ERN1ENSG00000178607.11632.87860996.74510-0.655306.218204.87320HepG2G3BP2
ERN1ENSG00000178607.11591.79730376.422300.652704.516703.71190HepG2GPKOW
ERN1ENSG00000178607.11569.688001009.20910-0.825006.494304.78900HepG2GRSF1
ERN1ENSG00000178607.11818.73490464.091200.8189014.7140012.75650HepG2HDGF
ERN1ENSG00000178607.11576.55870384.531600.584302.649201.31800HepG2HLTF
ERN1ENSG00000178607.111307.12870922.188900.503203.126501.67220HepG2HNRNPA0
ERN1ENSG00000178607.11665.18140462.252800.525005.471304.23440HepG2HNRNPA2B1
ERN1ENSG00000178607.11309.50250414.89770-0.422802.123301.50660HepG2HNRNPC
ERN1ENSG00000178607.111640.81540944.288200.797007.321804.92490HepG2HNRNPD
ERN1ENSG00000178607.11523.00530346.631100.593305.923804.92700HepG2HNRNPF
ERN1ENSG00000178607.11654.79570458.174500.515005.914004.96040HepG2HNRNPL
ERN1ENSG00000178607.11588.64250359.227000.712405.700304.83080HepG2HNRNPLL
ERN1ENSG00000178607.11518.01800341.332700.601705.284104.31620HepG2IGF2BP1
ERN1ENSG00000178607.11538.12240895.62370-0.735002.855601.85830HepG2IGF2BP3
ERN1ENSG00000178607.11579.02570421.001600.459704.747603.80940HepG2ILF2
ERN1ENSG00000178607.111895.260501062.817600.834407.486805.24380HepG2ILF3
ERN1ENSG00000178607.111378.854901039.115200.408002.508601.38750HepG2KHDRBS1
ERN1ENSG00000178607.11584.85230444.362200.396203.677702.50300HepG2KIF1C
ERN1ENSG00000178607.11545.253301012.47670-0.8929010.185808.95150HepG2MAGOH
ERN1ENSG00000178607.11470.730901018.15650-1.1130013.5143012.19310HepG2MATR3
ERN1ENSG00000178607.11510.21350380.569200.422802.727901.51920HepG2NAA15
ERN1ENSG00000178607.11693.252901042.15040-0.588104.857104.03100HepG2NCBP2
ERN1ENSG00000178607.11454.36200264.860900.778503.029001.88110HepG2NONO
ERN1ENSG00000178607.11539.17470318.525500.759205.973304.90030HepG2NSUN2
ERN1ENSG00000178607.113383.91440996.493101.7637023.8825021.34690HepG2PABPC1
ERN1ENSG00000178607.111575.97240928.151700.763706.889205.75830HepG2PABPC4
ERN1ENSG00000178607.11316.53240407.95870-0.366102.231801.46340HepG2PARN
ERN1ENSG00000178607.112220.29470977.684301.1832012.145809.23380HepG2PNPT1
ERN1ENSG00000178607.11582.21530367.608300.663307.355406.19560HepG2PPIL4
ERN1ENSG00000178607.11578.93110222.325701.3806015.0178013.35140HepG2PRPF8
ERN1ENSG00000178607.11497.75270877.01680-0.8172013.8737012.83220HepG2PTBP1
ERN1ENSG00000178607.111073.23280505.405701.0863032.0710030.50200HepG2PUM1
ERN1ENSG00000178607.111213.23720505.036201.2643029.5561027.52310HepG2PUM2
ERN1ENSG00000178607.11725.38590495.114800.550906.993906.09120HepG2PUS1
ERN1ENSG00000178607.11771.94370366.516501.074507.461006.33530HepG2RAVER1
ERN1ENSG00000178607.11748.97590596.688300.327802.382501.58070HepG2RBM15
ERN1ENSG00000178607.111216.88830862.045200.497305.282104.37860HepG2RBM25
ERN1ENSG00000178607.111538.523401052.998200.546903.633501.88370HepG2RBM47
ERN1ENSG00000178607.11353.22780976.04480-1.4663038.1668036.66420HepG2NELFE
ERN1ENSG00000178607.11479.97570909.03620-0.9214017.4094016.07980HepG2RECQL
ERN1ENSG00000178607.11609.43150373.406000.706606.139605.17880HepG2RPLP0
ERN1ENSG00000178607.11559.96380360.030100.637103.438002.38520HepG2RPS10
ERN1ENSG00000178607.11307.46650438.70150-0.512802.914102.09470HepG2RPS2
ERN1ENSG00000178607.11770.698301095.01530-0.506703.126402.27740HepG2RPS3A
ERN1ENSG00000178607.11539.95690219.916201.2958012.0360010.58160HepG2RRP9
ERN1ENSG00000178607.11524.10610367.040100.513804.922104.10410HepG2SAFB2
ERN1ENSG00000178607.111978.548001031.137500.9401011.343508.86480HepG2SART3
ERN1ENSG00000178607.111005.15730623.238100.689503.222802.49400HepG2SF3A3
ERN1ENSG00000178607.111255.78480355.562101.8203027.9221025.95030HepG2SF3B1
ERN1ENSG00000178607.111074.19040913.107700.234301.875901.37290HepG2SF3B4
ERN1ENSG00000178607.11764.88610456.510500.7445012.4121011.10810HepG2SLBP
ERN1ENSG00000178607.11737.48890357.600501.044205.186303.06890HepG2SLTM
ERN1ENSG00000178607.11624.14340395.625800.657603.519902.63380HepG2SNRNP70
ERN1ENSG00000178607.111696.77780758.894801.1607042.0405040.69150HepG2SRFBP1
ERN1ENSG00000178607.11466.90290953.07860-1.0295022.0651021.00280HepG2SRSF1
ERN1ENSG00000178607.11645.77090470.083800.458004.471003.70960HepG2SRSF5
ERN1ENSG00000178607.11602.40530340.553400.8228011.3094010.06710HepG2SRSF7
ERN1ENSG00000178607.111562.69080775.373201.0110032.4600030.97450HepG2SRSF9
ERN1ENSG00000178607.11496.48290658.90380-0.408303.754103.17730HepG2SSB
ERN1ENSG00000178607.11685.65910374.059400.874107.778005.84370HepG2STAU1
ERN1ENSG00000178607.11843.99550342.518401.3010012.349009.98410HepG2SUB1
ERN1ENSG00000178607.11739.54000378.150600.9676013.8026011.82360HepG2SUPT6H
ERN1ENSG00000178607.11575.08070350.142700.715706.087105.07060HepG2TARDBP
ERN1ENSG00000178607.11726.057001124.97460-0.631702.639201.59350HepG2TBRG4
ERN1ENSG00000178607.11355.69780192.350300.886805.269303.95900HepG2TFIP11
ERN1ENSG00000178607.11277.05560400.47420-0.531503.674103.11530HepG2TIAL1
ERN1ENSG00000178607.11732.13100465.134100.654406.728305.27400HepG2TUFM
ERN1ENSG00000178607.111462.62210479.020301.6103036.1656033.84710HepG2U2AF1
ERN1ENSG00000178607.11818.91500387.955201.077703.396102.29820HepG2U2AF2
ERN1ENSG00000178607.111038.59900492.337801.0768012.5978010.76460HepG2UBE2L3
ERN1ENSG00000178607.11785.27960552.718800.506605.596004.65740HepG2UPF1
ERN1ENSG00000178607.11713.66390533.625300.419303.961703.26390HepG2UTP3
ERN1ENSG00000178607.11610.61170437.095800.482205.080604.21810HepG2ZC3H8
ERN1ENSG00000178607.113072.49930607.882102.33740225.92070223.26740HepG2ZNF622
ERN1ENSG00000178607.111631.989802567.40160-0.6537017.3484016.04810K562AGGF1
ERN1ENSG00000178607.111468.090302671.49260-0.8637016.4785014.64510K562AKAP8L
ERN1ENSG00000178607.11589.48940875.27650-0.570304.494702.85250K562APOBEC3C
ERN1ENSG00000178607.111848.988302803.02930-0.6003012.5955010.47470K562ATP5C1
ERN1ENSG00000178607.112546.033803068.20220-0.269102.727301.56260K562CCDC124
ERN1ENSG00000178607.11558.67270361.562800.627705.404304.31620K562CPSF6
ERN1ENSG00000178607.112075.038503012.26440-0.537706.583004.32190K562DDX19B
ERN1ENSG00000178607.112446.722402874.74920-0.232602.562201.72430K562DKC1
ERN1ENSG00000178607.111376.37740509.601301.4333045.6177043.42050K562EIF2S2
ERN1ENSG00000178607.11364.44560464.64370-0.350402.165401.45520K562EIF3G
ERN1ENSG00000178607.11932.90500587.113100.668007.500006.15360K562EIF4A3
ERN1ENSG00000178607.11667.91370991.95090-0.570607.717106.16140K562EIF4G2
ERN1ENSG00000178607.11318.39820473.29590-0.571903.940402.91750K562FIP1L1
ERN1ENSG00000178607.11447.64560610.57400-0.447803.774303.08440K562FXR1
ERN1ENSG00000178607.11480.75380679.07850-0.498303.780102.90090K562GRSF1
ERN1ENSG00000178607.111469.98910641.666901.1958022.6683020.58430K562HSPD1
ERN1ENSG00000178607.111861.205402835.13920-0.607203.477602.54090K562KIF1C
ERN1ENSG00000178607.11479.86440750.19290-0.644607.109005.53620K562MATR3
ERN1ENSG00000178607.112514.509203176.06950-0.337004.039402.90700K562MBNL1
ERN1ENSG00000178607.11425.87540676.44530-0.667506.740205.52680K562MTPAP
ERN1ENSG00000178607.111515.819002226.19310-0.554504.334703.15550K562NAA15
ERN1ENSG00000178607.11624.37210751.60720-0.267602.313201.62480K562NSUN2
ERN1ENSG00000178607.11494.08040661.98910-0.422102.454801.67260K562PCBP3
ERN1ENSG00000178607.11372.97940507.77950-0.445103.145702.28790K562POLR2G
ERN1ENSG00000178607.11442.12340730.39150-0.724208.738907.60050K562PTBP1
ERN1ENSG00000178607.111192.936302649.42420-1.1512024.7011023.08320K562PUS1
ERN1ENSG00000178607.111165.403102581.43450-1.1473038.0134035.73450K562RBM15
ERN1ENSG00000178607.11347.43800736.91360-1.0847014.3457011.88800K562NELFE
ERN1ENSG00000178607.111690.340001000.528200.756502.767501.56310K562SF1
ERN1ENSG00000178607.111956.602301148.987500.767903.575602.11130K562SNRNP200
ERN1ENSG00000178607.11478.10850759.80480-0.668309.764908.12000K562SRPK2
ERN1ENSG00000178607.11521.55240716.05270-0.457303.689502.87850K562SRSF1
ERN1ENSG00000178607.11879.04910676.320900.378102.740701.69820K562SRSF5
ERN1ENSG00000178607.11400.09890509.30040-0.348202.106001.38870K562SYNCRIP
ERN1ENSG00000178607.11555.52690724.63720-0.383402.479201.80720K562TARDBP
ERN1ENSG00000178607.11900.94250699.008800.366002.596101.80600K562TIA1
ERN1ENSG00000178607.11627.46050463.576300.436603.461802.56460K562U2AF2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for ERN1 is not available on ENCORE database.





4 RBPs can affect the splicing of ERN1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEERN1ENSG00000178607.11chr17-621264146212656262125217621253456213013962130339-0.434000.000020.00081excludedHepG2U2AF1
SEERN1ENSG00000178607.11chr17-6214554962145651621440306214429262149340621494630.599000.000080.00164includedHepG2U2AF2
SEERN1ENSG00000178607.11chr17-621455496214565162144030621442926214934062149463-0.217000.003700.03629excludedK562AQR
SEERN1ENSG00000178607.11chr17-6214554962145651621440306214429262149340621494630.567000.000940.08792includedHepG2RBFOX2
MXEERN1ENSG00000178607.11chr17-62144030621442926214554962145651621425686214264762149340621494630.398000.019930.092361includedHepG2U2AF2

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for ERN1 is not avaliable on ENCORE database.