EIF2B3

General Information

Gene Name       : EIF2B3
Gene ID             : ENSG00000070785.16
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: EIF2B3)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878EIF2B3ENSG00000070785.1634.4127.0030.70ENCSR000AEF
HepG2EIF2B3ENSG00000070785.1622.7429.9826.36ENCSR985KAT
K562EIF2B3ENSG00000070785.1692.2699.4495.85ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq EIF2B3-BGKcLV15-K562ENCSR786OLNEIF2B3K562ReleasedENCSR404OHQ   CRISPRKD% = 83.7,86.9
knockdown followed by RNA-seq EIF2B3-BGHcLV16-HepG2ENCSR312UYNEIF2B3HepG2ReleasedENCSR964HKT   CRISPRKD% = 71.0,74.1






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
EIF2B3ENCAB985GAJMA1-078NL176595Released, ENCODE4BGHcLV16-23, Released, ENCODE4BGKcLV15-27, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
EIF2B3K562CRISPRBGKcLV13-29,BGKcLV13-30BGKcLV13-1,BGKcLV13-2NotSatisfiedENCODE4
EIF2B3K562CRISPRBGKcLV13-31,BGKcLV13-32BGKcLV13-1,BGKcLV13-2NotSatisfiedENCODE4
EIF2B3K562CRISPRBGKcLV15-25,BGKcLV15-26BGKcLV15-1,BGKcLV15-253.0,53.9NotSatisfiedENCODE4
EIF2B3K562CRISPRENCSR786OLNENCSR404OHQEIF2B3-BGKcLV15-27,EIF2B3-BGKcLV15-28NT-BGKcLV15-1,NT-BGKcLV15-283.7,86.9ReleasedENCODE4
EIF2B3HepG2CRISPRBGHcLV14-29,BGHcLV14-30BGHcLV14-1,BGHcLV14-20.0,0.0NotSatisfiedENCODE4
EIF2B3HepG2CRISPRBGHcLV14-31,BGHcLV14-32BGHcLV14-1,BGHcLV14-20.0,0.0NotSatisfiedENCODE4
EIF2B3HepG2CRISPRENCSR312UYNENCSR964HKTEIF2B3-BGHcLV16-23,EIF2B3-BGHcLV16-24NT-BGHcLV16-1,NT-BGHcLV16-271.0,74.1ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2B3Product_ID: MA1-078
Lot_ID: NL176595
Source: Thermo Fisher
Target Name: EIF2B3-human		 EIF2B3-HEPG2-CRISPR-MA1-078.png<br>Caption: Western blot following CRISPR against EIF2B3 in HepG2 whole cell lysate using EIF2B3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B3. EIF2B3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_MA1-078_NL176595_EIF2B3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF2B3 specific antibody, MA1-078. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF2B3-specific antibody, MA1-078. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF2B3-K562-CRISPR-MA1-078.png<br>Caption: Western blot following CRISPR against EIF2B3 in K562 whole cell lysate using EIF2B3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B3. EIF2B3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for EIF2B3 is not avaliable on ENCORE database.



Proteins bound to RNA
  • EIF2B3 RNA is bound by 18 proteins.  







eCLIP assay for EIF2B3 is not avaliable on ENCORE database.





18 proteins are bound to EIF2B3 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr14531649245316595-3'-UTR,intron-Distal5.769258.52455K562PABPC4
chr14531656945316656-3'-UTR,CDS,intron-Distal3.373735.39816K562LIN28B
chr14545203045452168-5'-UTR,intron-Distal4.846024.86944K562AQR
chr14545216245452258-5'-UTR,intron-Distal5.1229937.27212K562DDX3X
chr14544683745446850-5'-UTR,CDS,intron-Distal,intron-Proximal5.8462010.20835K562DDX3X
chr14539236045392417-CDS,intron-Distal,intron-Proximal4.238874.39669K562BUD13
chr14540727145407278-CDS,intron-Distal4.7106712.49278K562YBX3
chr14534551545345565-CDS,intron-Distal3.774803.22245K562GRWD1
chr14532347745323525-CDS,intron-Distal,intron-Proximal3.731044.84442HepG2SF3B4
chr14534550345345592-CDS,intron-Distal3.175956.65384K562UCHL5
chr14539229945392322-CDS,intron-Distal,intron-Proximal3.822813.67607K562YBX3
chr14540730645407332-CDS,intron-Distal4.6285313.15557K562YBX3
chr14540725045407271-CDS,intron-Distal3.487334.30677K562YBX3
chr14534553745345597-CDS,intron-Distal4.100484.12139K562DDX24
chr14540727845407300-CDS,intron-Distal4.9271015.33972K562YBX3
chr14539231145392357-CDS,intron-Distal,intron-Proximal4.696135.14565K562UCHL5
chr14534733345347372-CDS,intron-Distal3.331565.04943K562ZNF622
chr14534559445345667-CDS,intron-Distal3.181925.97026K562PUM1
chr14544399745444062-CDS,intron-Distal4.895835.50697K562UCHL5
chr14544406245444126-CDS,intron-Distal3.455625.76403K562UCHL5
chr14540721145407335-CDS,intron-Distal4.453673.47885K562LIN28B
chr14542555345425718-intron-Distal4.131284.70117K562HNRNPL
chr14544225145442345-intron-Distal3.988773.25538K562KHSRP
chr14537476045374848-intron-Distal4.865407.11021HepG2PTBP1
chr14538863945388769-intron-Distal3.366954.70071K562SAFB2
chr14537476845374842-intron-Distal3.335403.68462HepG2MATR3
chr14537477245374856-intron-Distal4.6011717.68778K562PTBP1
chr14540570845405840-intron-Distal4.401585.25174K562KHSRP
chr14544184045441976-intron-Distal4.292263.71397K562KHSRP
chr14543944445439560-intron-Distal4.950135.89032HepG2QKI
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for EIF2B3 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for EIF2B3 is not available on ENCORE database.

  • 103 RBPs can affect the levels of EIF2B3.  








The Knockdown/knockout RNA-Seq assay for EIF2B3 is not available on ENCORE database.





103 RBPs can affect the gene expression of EIF2B3 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
EIF2B3ENSG00000070785.12341.51870270.503100.336201.840201.32620HepG2ACO1
EIF2B3ENSG00000070785.12971.32480504.151400.946009.462308.54270HepG2AKAP1
EIF2B3ENSG00000070785.12275.58410417.14090-0.598003.488302.60710HepG2ASCC1
EIF2B3ENSG00000070785.12163.68700257.51030-0.653703.554402.16810HepG2BUD13
EIF2B3ENSG00000070785.12212.56740396.31320-0.898706.677005.50970HepG2CIRBP
EIF2B3ENSG00000070785.12216.11160303.18660-0.488402.453801.59800HepG2DDX24
EIF2B3ENSG00000070785.12190.43090301.12750-0.661102.459301.53820HepG2DDX59
EIF2B3ENSG00000070785.12168.55870245.43060-0.542102.787402.08260HepG2DNAJC21
EIF2B3ENSG00000070785.12289.03510398.80560-0.464402.257901.71720HepG2EIF2S1
EIF2B3ENSG00000070785.12674.68850537.529800.327802.142701.39050HepG2EIF4G1
EIF2B3ENSG00000070785.12338.76830514.89320-0.604003.277402.39510HepG2EIF4G2
EIF2B3ENSG00000070785.12425.81110601.88100-0.499305.046804.27870HepG2ETF1
EIF2B3ENSG00000070785.12163.21130295.17130-0.854805.912304.34490HepG2FASTKD2
EIF2B3ENSG00000070785.12230.04040412.50290-0.842506.028305.06360HepG2G3BP1
EIF2B3ENSG00000070785.12284.31480437.93540-0.623203.913402.84260HepG2G3BP2
EIF2B3ENSG00000070785.12120.10740180.94400-0.591202.324901.51130HepG2GEMIN5
EIF2B3ENSG00000070785.12221.83900317.87700-0.519002.507201.54350HepG2GNB2L1
EIF2B3ENSG00000070785.12133.96820300.59040-1.165907.902906.85720HepG2GPKOW
EIF2B3ENSG00000070785.12298.65220408.85940-0.453103.229502.45990HepG2HNRNPF
EIF2B3ENSG00000070785.12308.29410417.71570-0.438202.191901.56450HepG2HNRNPK
EIF2B3ENSG00000070785.12163.71760286.85960-0.809104.500003.71630HepG2HNRNPLL
EIF2B3ENSG00000070785.12282.52250402.60860-0.511003.424802.62570HepG2IGF2BP1
EIF2B3ENSG00000070785.12258.47260419.29720-0.698004.382703.54960HepG2IGF2BP2
EIF2B3ENSG00000070785.12279.41410421.74680-0.594002.901501.87240HepG2KHSRP
EIF2B3ENSG00000070785.12263.32430444.84090-0.756405.262504.36400HepG2MAGOH
EIF2B3ENSG00000070785.12174.52900292.57490-0.745303.059202.03690HepG2MTPAP
EIF2B3ENSG00000070785.12316.09150540.13760-0.773005.229104.18600HepG2NOL12
EIF2B3ENSG00000070785.12172.19200326.58250-0.923404.356902.96560HepG2NONO
EIF2B3ENSG00000070785.12173.71930254.36100-0.550102.231101.55690HepG2NSUN2
EIF2B3ENSG00000070785.12265.35630407.74750-0.619703.694902.86860HepG2PABPC4
EIF2B3ENSG00000070785.12194.43320274.26410-0.496302.083501.37830HepG2PRPF8
EIF2B3ENSG00000070785.12237.38250515.65520-1.119208.912507.92480HepG2PUF60
EIF2B3ENSG00000070785.12324.92420547.01150-0.751504.995504.10820HepG2PUM2
EIF2B3ENSG00000070785.12361.22190461.06970-0.352102.061901.51090HepG2QKI
EIF2B3ENSG00000070785.12332.75300503.05080-0.596305.329204.66880HepG2RBM22
EIF2B3ENSG00000070785.12312.20510473.76920-0.601705.076004.37970HepG2NELFE
EIF2B3ENSG00000070785.12326.88990441.22840-0.432702.988402.35070HepG2RECQL
EIF2B3ENSG00000070785.12279.96130440.45870-0.653804.784603.91880HepG2RPLP0
EIF2B3ENSG00000070785.12196.56120283.93020-0.530602.485801.81460HepG2SF3B1
EIF2B3ENSG00000070785.12280.95120443.22980-0.657705.800604.99770HepG2SF3B4
EIF2B3ENSG00000070785.12339.58960494.54480-0.542302.950002.22750HepG2SLBP
EIF2B3ENSG00000070785.12137.39770307.39860-1.161803.460901.95910HepG2SNRNP200
EIF2B3ENSG00000070785.12433.52040568.15900-0.390203.198202.67250HepG2SRFBP1
EIF2B3ENSG00000070785.12179.46370305.52150-0.767603.647602.48300HepG2SRSF3
EIF2B3ENSG00000070785.12337.31220509.19970-0.594103.497302.80870HepG2SRSF5
EIF2B3ENSG00000070785.12291.74510401.69470-0.461403.350002.59420HepG2SRSF7
EIF2B3ENSG00000070785.12249.36570376.36020-0.593904.951404.25270HepG2SRSF9
EIF2B3ENSG00000070785.12373.00240493.26460-0.403203.156102.61660HepG2SSB
EIF2B3ENSG00000070785.12218.88420353.97880-0.693503.821103.04570HepG2SUPV3L1
EIF2B3ENSG00000070785.12315.07930415.79390-0.400202.493001.95700HepG2TAF15
EIF2B3ENSG00000070785.12169.23850279.61800-0.724403.915103.07000HepG2TARDBP
EIF2B3ENSG00000070785.12193.41330266.02470-0.459902.185301.73050HepG2TIAL1
EIF2B3ENSG00000070785.12193.01650268.42100-0.475802.684302.12690HepG2TRIM56
EIF2B3ENSG00000070785.12290.65310209.095800.475002.053001.39930HepG2UCHL5
EIF2B3ENSG00000070785.12367.19670227.965900.687605.455404.66150HepG2UTP3
EIF2B3ENSG00000070785.12377.63550259.688600.540104.383103.73640HepG2ZNF622
EIF2B3ENSG00000070785.122016.046201690.068000.254303.043402.45220K562AGGF1
EIF2B3ENSG00000070785.121873.624103456.39080-0.8834017.4702016.15950K562AQR
EIF2B3ENSG00000070785.122273.484401845.163800.301103.541302.49610K562ATP5C1
EIF2B3ENSG00000070785.121768.073902156.44650-0.286503.362501.88630K562BCCIP
EIF2B3ENSG00000070785.121753.007102083.87450-0.249403.122502.10740K562BCLAF1
EIF2B3ENSG00000070785.12810.619101249.21140-0.623904.869503.69890K562BOP1
EIF2B3ENSG00000070785.121409.493001900.68110-0.431303.445801.82510K562BUD13
EIF2B3ENSG00000070785.12533.94890808.11080-0.597905.631203.98740K562CCAR1
EIF2B3ENSG00000070785.121677.072902174.30700-0.374603.486202.03650K562CIRBP
EIF2B3ENSG00000070785.121435.092802527.11070-0.816303.152002.00760K562CNOT7
EIF2B3ENSG00000070785.122156.626402529.02550-0.229802.503601.45460K562CPEB4
EIF2B3ENSG00000070785.12914.958801328.28560-0.537806.498305.29540K562CPSF6
EIF2B3ENSG00000070785.123629.283302617.202500.4716010.992609.79160K562DDX47
EIF2B3ENSG00000070785.122277.903201892.412000.267403.058002.13070K562DKC1
EIF2B3ENSG00000070785.12910.290901776.85770-0.9649010.100208.24780K562EFTUD2
EIF2B3ENSG00000070785.121579.452002684.41960-0.7652021.0337019.32530K562EIF2S2
EIF2B3ENSG00000070785.121329.554801902.81130-0.517203.885302.63440K562EIF3D
EIF2B3ENSG00000070785.122108.320401444.160900.545809.264607.81380K562EIF3G
EIF2B3ENSG00000070785.121093.787801582.68350-0.533006.250705.02680K562EIF4A3
EIF2B3ENSG00000070785.123031.064703906.96300-0.366205.859404.51430K562EIF4G2
EIF2B3ENSG00000070785.12805.467901351.46920-0.746609.558907.91900K562FMR1
EIF2B3ENSG00000070785.12722.182801145.47910-0.665506.599705.12780K562FTO
EIF2B3ENSG00000070785.122026.312801645.912100.299903.497002.82860K562FXR1
EIF2B3ENSG00000070785.121312.554901781.38430-0.440603.407501.80440K562GEMIN5
EIF2B3ENSG00000070785.12784.881001268.19810-0.692208.817107.39250K562GPKOW
EIF2B3ENSG00000070785.12623.256601633.49170-1.3901028.9277027.08380K562HNRNPC
EIF2B3ENSG00000070785.121039.657101458.51680-0.488405.179103.55440K562HNRNPF
EIF2B3ENSG00000070785.122266.113501613.458000.490004.368603.18240K562HNRNPM
EIF2B3ENSG00000070785.122177.031902749.06830-0.336604.474603.33110K562HNRNPU
EIF2B3ENSG00000070785.12669.571601082.85070-0.693505.471804.10270K562KRR1
EIF2B3ENSG00000070785.121687.532902132.08950-0.337403.208302.29850K562MAGOH
EIF2B3ENSG00000070785.12823.620601237.89770-0.587806.769405.36590K562METAP2
EIF2B3ENSG00000070785.121864.908902191.81060-0.233003.768202.91290K562NSUN2
EIF2B3ENSG00000070785.121366.485101897.12330-0.473305.301403.93410K562PCBP2
EIF2B3ENSG00000070785.121213.078601815.87020-0.582005.867704.65110K562PCBP3
EIF2B3ENSG00000070785.121057.839001392.85200-0.396903.962502.99860K562POLR2G
EIF2B3ENSG00000070785.121030.282601464.54260-0.507405.177303.88900K562PSIP1
EIF2B3ENSG00000070785.122615.948702071.421000.336603.645602.89450K562PTBP1
EIF2B3ENSG00000070785.123025.597304262.80740-0.494606.493105.24300K562PUF60
EIF2B3ENSG00000070785.123162.635604442.58810-0.4903010.414908.52490K562PUM2
EIF2B3ENSG00000070785.122369.633801744.089400.442104.565803.89170K562PUS1
EIF2B3ENSG00000070785.122045.465101699.305900.267402.720002.13120K562RBM15
EIF2B3ENSG00000070785.121486.800801986.51950-0.418004.128603.07950K562RBM22
EIF2B3ENSG00000070785.121009.849701335.94630-0.403704.034002.83590K562RCC2
EIF2B3ENSG00000070785.121760.972001422.444600.307904.552702.52540K562RPS10
EIF2B3ENSG00000070785.121532.671202162.76190-0.496808.955207.54690K562SF3A3
EIF2B3ENSG00000070785.12662.269501206.05650-0.864809.770707.85860K562SF3B1
EIF2B3ENSG00000070785.121193.122301729.26030-0.535407.692106.22470K562SF3B4
EIF2B3ENSG00000070785.121018.952501903.59560-0.901603.701002.20230K562SNRNP200
EIF2B3ENSG00000070785.12995.793001266.46820-0.346902.562501.58660K562SRFBP1
EIF2B3ENSG00000070785.121699.323502215.79670-0.382907.563906.09530K562SRPK2
EIF2B3ENSG00000070785.121116.312902030.62680-0.8632017.6480015.88340K562SRSF1
EIF2B3ENSG00000070785.12706.187801103.19570-0.643607.642505.76020K562SRSF4
EIF2B3ENSG00000070785.121052.460701674.55620-0.670005.002003.60190K562SUPV3L1
EIF2B3ENSG00000070785.121768.566701397.042500.340103.550402.62020K562SYNCRIP
EIF2B3ENSG00000070785.121309.728602055.24820-0.650005.790204.74350K562TARDBP
EIF2B3ENSG00000070785.121671.978801982.48710-0.245802.024801.33160K562TIA1
EIF2B3ENSG00000070785.12896.915501408.67650-0.651306.692905.18220K562TIAL1
EIF2B3ENSG00000070785.121576.896301945.12210-0.302802.370001.37580K562TRA2A
EIF2B3ENSG00000070785.121114.137101496.24750-0.425403.931002.76170K562TRIM56
EIF2B3ENSG00000070785.121113.420501664.77390-0.580308.751707.31170K562U2AF2
EIF2B3ENSG00000070785.121077.581701772.09420-0.717706.617204.96630K562UCHL5
EIF2B3ENSG00000070785.121567.694302324.02350-0.5680013.8132012.35590K562UPF1
EIF2B3ENSG00000070785.121490.953801988.59320-0.415504.725903.56740K562WDR3
EIF2B3ENSG00000070785.122340.021002067.908700.178202.077901.33660K562WDR43
EIF2B3ENSG00000070785.121376.374201804.14440-0.390402.756701.55290K562XRN2
EIF2B3ENSG00000070785.122976.132503893.99380-0.387804.378403.25130K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for EIF2B3 is not available on ENCORE database.





12 RBPs can affect the splicing of EIF2B3 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEEIF2B3ENSG00000070785.12chr1-453233754532347945316449453166754534034945340498-0.079000.001180.02395excludedK562SRSF1
SEEIF2B3ENSG00000070785.12chr1-4536302645363107453472834534741145392299453923270.085000.000410.07731includedK562FTO
SEEIF2B3ENSG00000070785.12chr1-453630264536311645347283453474114539229945392327-0.117000.000080.00164excludedHepG2U2AF2
SEEIF2B3ENSG00000070785.12chr1-453630264536310745347283453474114539229945392327-0.058000.011350.08980excludedK562AQR
SEEIF2B3ENSG00000070785.12chr1-453630264536311645347283453474114539229945392411-0.113000.000460.06164excludedHepG2HNRNPUL1
SEEIF2B3ENSG00000070785.12chr1-453630264536311645347283453474114539229945392411-0.079000.000020.01107excludedK562FXR2
SEEIF2B3ENSG00000070785.12chr1-453630264536310745347283453474114539229945392327-0.071000.000950.03303excludedK562UTP18
MXEEIF2B3ENSG00000070785.12chr1-45347283453474114536302645363116453454894534568045392299453923270.181000.000000.000001includedK562DROSHA
MXEEIF2B3ENSG00000070785.12chr1-45347283453474114536302645363116453454894534568045392299453923270.124000.003980.075041includedK562SBDS
MXEEIF2B3ENSG00000070785.12chr1-4534548945345680453472834534741145341289453413674536302645363116-0.129000.001870.065812includedK562SAFB
MXEEIF2B3ENSG00000070785.12chr1-45347283453474114536302645363116453454894534568045392299453923270.163000.000130.007781includedK562PPIL4
MXEEIF2B3ENSG00000070785.12chr1-45340349453404984534128945341367453233754532347945345489453456800.121000.003780.092161includedK562DDX55
MXEEIF2B3ENSG00000070785.12chr1-45347283453474114536302645363116453454894534568045392299453924110.165000.000000.000071includedK562HNRNPUL1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for EIF2B3 is not avaliable on ENCORE database.