EIF2B3      CRISPR in K562      Control: NT-BGKcLV15

General Information
RBPEIF2B3
Cell_LineK562
MethodCRISPR
Exp_NameEIF2B3-BGKcLV15-K562
ENCODE_series_IDENCSR513MXV
Batch_IDBGKcLV15
Pool IDPool-200102
Local_Set_Nameset43_K
ENCODE_KD_Exp_IDENCSR786OLN
ENCODE_CN_Exp_IDENCSR404OHQ
Rep1EIF2B3-BGKcLV15-27
Rep2EIF2B3-BGKcLV15-28
CN1NT-BGKcLV15-1
CN2NT-BGKcLV15-2
Rep1_qPCR53.3
Rep2_qPCR56.1
Rep1_WB83.7
Rep2_WB86.9
Antibody Cat#MA1-078
Antibody Lot#LOT# NL176595
Antibody DCC IDENCAB985GAJ
StatusReleased
ProjectENCODE4
ID125




Experiment Information (Status: NotSatisfied)
BGKcLV15-25BGKcLV15-26
idx561562
TRCN#_or_BGC#BGC#0000389BGC#0000389
shRNA_or_gRNA_sequenceAACCTGTTCCCGGTCAAAAGAACCTGTTCCCGGTCAAAAG
PAMGGGGGG
NameEIF2B gamma_85EIF2B gamma_85
Sample_IDBGKcLV15-25BGKcLV15-26
transduction_Date4/2/194/2/19
daysDay 6Day 6
RBP_nameEIF2B3EIF2B3
qPCR_result71.364.4
Ave_qPCR67.9
RT-qPCR_primer-Ftggtacccattgaacctgct
RT-qPCR_primer-Rccttttgaacatccctggtt
protein_conc61225749
WB_result53.053.9
Ave_WB53.5
WB_DONE_date4/21/19
MW58KD
IPMouse / Ig
antibody_Cat#MA1-078LOT# NL176595
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM100.60
Rep2_TPM34.10
Action
Library_start_date
repeat_library
Note
ID46714672




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV15-25BGKcLV15-26
Sample_IDBGKcLV15-25BGKcLV15-26
Sample Name
Sample Name
RBPEIF2B3EIF2B3
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2020-09-122020-09-12
ProjectENCODE4ENCODE4
Note
ID53935394




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database