SAMHD1

General Information

Gene Name       : SAMHD1
Gene ID             : ENSG00000101347.9
Essential Gene : No

Functions

Localization

Domains (AlphaFold: SAMHD1)


Antibody Validation

 View validation images for all SAMHD1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878SAMHD1ENSG00000101347.928.3428.4528.39ENCSR000AEF
HepG2SAMHD1ENSG00000101347.927.9731.1329.55ENCSR985KAT
K562SAMHD1ENSG00000101347.911.6112.7012.15ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq SAMHD1-BGKcLV33-K562ENCSR870EBUSAMHD1K562ReleasedENCSR271ZAB   CRISPRKD% = 69.0,73.3
knockdown followed by RNA-seq SAMHD1-BGHcLV31-HepG2ENCSR878TRKSAMHD1HepG2ReleasedENCSR452TFV   CRISPRKD% = 71.8,72.4






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
SAMHD1ENCAB434HZOA303-690A1BGHcLV31-23, Released, ENCODE4BGKcLV33-27, Released, ENCODE4
SAMHD1ENCAB065TLLA303-691A1BGHcLV31-23, Released, ENCODE4BGKcLV33-27, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
SAMHD1K562CRISPRBGKcLV33-21,BGKcLV33-22BGKcLV33-1,BGKcLV33-262.1,72.2155534NotSatisfiedENCODE4
SAMHD1K562CRISPRENCSR870EBUENCSR271ZABSAMHD1-BGKcLV33-27,SAMHD1-BGKcLV33-28NT-BGKcLV33-1,NT-BGKcLV33-269.0,73.3ReleasedENCODE4
SAMHD1HepG2CRISPRENCSR878TRKENCSR452TFVSAMHD1-BGHcLV31-23,SAMHD1-BGHcLV31-24NT-BGHcLV31-1,NT-BGHcLV31-271.8,72.4ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SAMHD1Product_ID: A303-690A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SAMHD1-human		 SAMHD1-HEPG2-CRISPR-A303-690A.png<br>Caption: Western blot following CRISPR against SAMHD1 in HepG2 whole cell lysate using SAMHD1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAMHD1. SAMHD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SAMHD1-K562-CRISPR-A303-690A.png<br>Caption: Western blot following CRISPR against SAMHD1 in K562 whole cell lysate using SAMHD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAMHD1. SAMHD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SAMHD1Product_ID: A303-691A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SAMHD1-human		 SAMHD1-K562-CRISPR-A303-691A.png<br>Caption: Western blot following CRISPR against SAMHD1 in K562 whole cell lysate using SAMHD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAMHD1. SAMHD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for SAMHD1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • SAMHD1 RNA is bound by 6 proteins.  







eCLIP assay for SAMHD1 is not avaliable on ENCORE database.





6 proteins are bound to SAMHD1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr203552046835520528-3'-UTR,intron-Distal3.928493.64119HepG2UPF1
chr203552020735520275-3'-UTR,intron-Distal3.520904.81659HepG2UPF1
chr203552074735520782-3'-UTR,intron-Distal3.136673.06830K562UPF1
chr203551917935519266-3'-UTR,intron-Distal6.3841810.19274K562PABPC4
chr203552073835520805-3'-UTR,intron-Distal4.928524.46951K562FAM120A
chr203552065535520729-3'-UTR,intron-Distal3.739893.66308K562UPF1
chr203552093635521021-3'-UTR,intron-Distal3.371824.74960HepG2UPF1
chr203557514035575204-5'-UTR,CDS,intron-Distal,intron-Proximal4.195873.16051HepG2PPIG
chr203554517535545239-CDS,intron-Distal,intron-Proximal4.422593.89826HepG2BCLAF1
chr203554535135545441-CDS,intron-Distal,intron-Proximal3.877104.51739HepG2BCLAF1
chr203554780035547881-CDS,intron-Distal3.427915.56491HepG2BCLAF1
chr203555262435552778-intron-Distal4.707265.75114HepG2PTBP1
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for SAMHD1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for SAMHD1 is not available on ENCORE database.

  • 120 RBPs can affect the levels of SAMHD1.  








The Knockdown/knockout RNA-Seq assay for SAMHD1 is not available on ENCORE database.





120 RBPs can affect the gene expression of SAMHD1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
SAMHD1ENSG00000101347.71853.758901124.804700.720708.847007.30700HepG2ABCF1
SAMHD1ENSG00000101347.7843.661501011.99830-0.262502.515701.93120HepG2ACO1
SAMHD1ENSG00000101347.71108.14630813.023700.446703.318202.23330HepG2ADAR
SAMHD1ENSG00000101347.71503.71550974.741200.625305.408404.69390HepG2AKAP1
SAMHD1ENSG00000101347.7399.22660784.92040-0.975309.936208.10130HepG2AKAP8
SAMHD1ENSG00000101347.7804.681601016.72030-0.337403.505002.65370HepG2AKAP8L
SAMHD1ENSG00000101347.7785.522801243.91440-0.663205.403504.08090HepG2BCCIP
SAMHD1ENSG00000101347.7425.024501080.91090-1.3466020.0216017.95780HepG2BCLAF1
SAMHD1ENSG00000101347.7827.184301447.37400-0.8072011.7374010.61920HepG2BOP1
SAMHD1ENSG00000101347.71300.84920766.265000.763409.053007.72330HepG2CIRBP
SAMHD1ENSG00000101347.71209.328701011.739000.257302.849602.17760HepG2CKAP4
SAMHD1ENSG00000101347.7451.584801328.77440-1.5570033.0160030.99960HepG2CPSF6
SAMHD1ENSG00000101347.7680.78000926.61330-0.444803.423702.19660HepG2CPSF7
SAMHD1ENSG00000101347.71009.961501339.06530-0.406906.256805.20660HepG2DAZAP1
SAMHD1ENSG00000101347.71351.02180935.935400.5295010.538709.11860HepG2DDX19B
SAMHD1ENSG00000101347.71255.19290796.818800.655507.846106.76220HepG2DDX21
SAMHD1ENSG00000101347.71058.32930779.348900.441303.377602.36260HepG2DDX24
SAMHD1ENSG00000101347.7678.800401223.00200-0.849408.471707.19900HepG2DDX55
SAMHD1ENSG00000101347.71041.140101508.91890-0.535402.614101.66620HepG2DDX59
SAMHD1ENSG00000101347.7678.716401004.13810-0.565102.821901.80010HepG2DDX5
SAMHD1ENSG00000101347.7274.73400801.99550-1.5456019.7904018.33810HepG2DDX6
SAMHD1ENSG00000101347.7454.24830818.71060-0.8499013.7027012.23370HepG2DNAJC21
SAMHD1ENSG00000101347.7511.16030811.40080-0.6666011.3925010.16890HepG2DNAJC2
SAMHD1ENSG00000101347.71491.07170681.730401.1290018.6720016.62540HepG2EEF2
SAMHD1ENSG00000101347.7531.08230771.12840-0.538003.822603.13290HepG2EIF2S1
SAMHD1ENSG00000101347.71249.36400752.200100.7319010.915409.06350HepG2EIF3G
SAMHD1ENSG00000101347.7704.791901265.59880-0.8446011.400109.81720HepG2EIF4G2
SAMHD1ENSG00000101347.71381.570801766.30920-0.354404.951004.18850HepG2ETF1
SAMHD1ENSG00000101347.71329.62200988.868300.427104.264403.22690HepG2EWSR1
SAMHD1ENSG00000101347.7375.98770494.58270-0.395502.546601.42680HepG2FAM120A
SAMHD1ENSG00000101347.7684.386901331.03900-0.959704.757003.43310HepG2FTO
SAMHD1ENSG00000101347.71156.75820859.674500.428103.101102.36850HepG2FXR1
SAMHD1ENSG00000101347.7430.58740797.59020-0.889304.258203.44130HepG2G3BP1
SAMHD1ENSG00000101347.7769.83030966.14480-0.327703.383902.31100HepG2HDGF
SAMHD1ENSG00000101347.71483.23690826.553200.8435013.1272011.89190HepG2HNRNPA1
SAMHD1ENSG00000101347.7955.246801230.71000-0.365504.009902.91780HepG2HNRNPA2B1
SAMHD1ENSG00000101347.7635.32630919.36100-0.533104.776003.88340HepG2HNRNPC
SAMHD1ENSG00000101347.71231.070201456.23920-0.242301.929101.32320HepG2HNRNPF
SAMHD1ENSG00000101347.7584.35420807.36150-0.466403.346102.58100HepG2HNRNPK
SAMHD1ENSG00000101347.7655.527501219.74150-0.8958023.3381021.57930HepG2HNRNPL
SAMHD1ENSG00000101347.7882.896801437.47540-0.703208.071307.05870HepG2HNRNPLL
SAMHD1ENSG00000101347.71512.07330913.284700.7273017.2368015.82460HepG2HNRNPU
SAMHD1ENSG00000101347.71619.15300854.554000.9219010.195207.93260HepG2HSPD1
SAMHD1ENSG00000101347.71040.83240810.774300.360301.973801.39490HepG2IGF2BP2
SAMHD1ENSG00000101347.7631.56240925.06890-0.550608.861807.12760HepG2KIF1C
SAMHD1ENSG00000101347.71085.24850859.973700.335602.037001.47020HepG2MAGOH
SAMHD1ENSG00000101347.71477.67850864.822700.772808.144207.10890HepG2MATR3
SAMHD1ENSG00000101347.7997.199501466.00060-0.555903.506002.40650HepG2MTPAP
SAMHD1ENSG00000101347.7562.43000772.09210-0.457103.493602.12620HepG2NAA15
SAMHD1ENSG00000101347.7632.427201327.82220-1.0701024.3474022.26590HepG2NOL12
SAMHD1ENSG00000101347.71002.733401274.58440-0.346102.369301.67530HepG2NSUN2
SAMHD1ENSG00000101347.7248.86310480.09150-0.948002.410201.59260HepG2PABPC1
SAMHD1ENSG00000101347.7573.85500788.48300-0.458402.403701.72660HepG2PABPC4
SAMHD1ENSG00000101347.7989.23480815.498200.278502.405801.83030HepG2PCBP1
SAMHD1ENSG00000101347.71386.25540952.198800.541803.312502.18000HepG2PCBP2
SAMHD1ENSG00000101347.7689.40280962.37610-0.481303.933801.88040HepG2PES1
SAMHD1ENSG00000101347.7596.06870814.58160-0.450604.674903.74320HepG2PPIL4
SAMHD1ENSG00000101347.71568.806101000.988500.648104.427602.90650HepG2PRPF6
SAMHD1ENSG00000101347.71382.88400829.758200.7368014.9249013.85110HepG2PTBP1
SAMHD1ENSG00000101347.7587.796901267.56300-1.1087020.7279019.29960HepG2PUF60
SAMHD1ENSG00000101347.71532.084201345.621600.187102.300801.74980HepG2PUM1
SAMHD1ENSG00000101347.71063.341801344.60920-0.338602.997502.29890HepG2PUM2
SAMHD1ENSG00000101347.71809.693901030.780200.8119024.5248023.02930HepG2PUS1
SAMHD1ENSG00000101347.7652.835301225.77680-0.9089017.4672015.62470HepG2RBM15
SAMHD1ENSG00000101347.7719.514501476.31150-1.0369025.7414024.48410HepG2RBM22
SAMHD1ENSG00000101347.71184.66090766.375400.628305.442703.94910HepG2RCC2
SAMHD1ENSG00000101347.71322.44050923.475900.518007.563506.74780HepG2NELFE
SAMHD1ENSG00000101347.71234.72150860.029500.521607.673106.72300HepG2RECQL
SAMHD1ENSG00000101347.71007.126201568.99490-0.639608.864007.74910HepG2RPLP0
SAMHD1ENSG00000101347.71128.41860870.369200.374502.449101.69270HepG2RPS2
SAMHD1ENSG00000101347.7896.50650527.031600.766304.457803.44280HepG2RPS3A
SAMHD1ENSG00000101347.71437.00140999.742500.523303.039701.43990HepG2RTF1
SAMHD1ENSG00000101347.7232.27850813.33610-1.8080053.4985051.23920HepG2SAFB2
SAMHD1ENSG00000101347.7737.12780540.507100.447503.161402.15340HepG2SERBP1
SAMHD1ENSG00000101347.7710.219501422.82660-1.0024016.6068015.03430HepG2SF3B1
SAMHD1ENSG00000101347.7474.31430863.96010-0.8651015.1696013.95200HepG2SF3B4
SAMHD1ENSG00000101347.71030.854701215.49280-0.237702.245701.60240HepG2SLBP
SAMHD1ENSG00000101347.7324.73560896.56540-1.465109.614006.89940HepG2SLTM
SAMHD1ENSG00000101347.7377.867101583.14050-2.0668032.7297030.08590HepG2SNRNP70
SAMHD1ENSG00000101347.7694.879201667.44640-1.2628049.5608048.13160HepG2SRFBP1
SAMHD1ENSG00000101347.72131.18280901.756801.2407050.4334048.97010HepG2SRSF1
SAMHD1ENSG00000101347.7951.760801430.76890-0.588108.635007.52210HepG2SRSF7
SAMHD1ENSG00000101347.7547.17400733.60100-0.423004.589603.91180HepG2SRSF9
SAMHD1ENSG00000101347.7373.70540758.92810-1.022109.530807.42360HepG2STAU1
SAMHD1ENSG00000101347.7505.96430858.71020-0.763105.002803.38020HepG2SUB1
SAMHD1ENSG00000101347.7476.64530767.21980-0.686707.012405.56490HepG2SUPT6H
SAMHD1ENSG00000101347.7873.772901217.51820-0.478603.162902.44470HepG2SUPV3L1
SAMHD1ENSG00000101347.7645.62190810.44960-0.328002.708602.15380HepG2TAF15
SAMHD1ENSG00000101347.71745.56390836.396301.061306.682005.10380HepG2TBRG4
SAMHD1ENSG00000101347.7561.94410744.78250-0.406403.314702.40370HepG2TIA1
SAMHD1ENSG00000101347.7560.16110887.52680-0.663908.615607.82230HepG2TIAL1
SAMHD1ENSG00000101347.7308.20110895.35320-1.5386045.1280043.21030HepG2TRIM56
SAMHD1ENSG00000101347.71877.35930971.803500.9499015.9208014.36590HepG2U2AF1
SAMHD1ENSG00000101347.7738.621601114.97540-0.594103.486902.35260HepG2UPF2
SAMHD1ENSG00000101347.71431.157401075.017800.412705.692404.23800HepG2UTP18
SAMHD1ENSG00000101347.7826.804301198.51200-0.535604.221403.33270HepG2XRN2
SAMHD1ENSG00000101347.71195.89490993.549900.267302.957002.26660HepG2ZC3H8
SAMHD1ENSG00000101347.7881.852501150.25590-0.383305.614804.90630HepG2ZNF622
SAMHD1ENSG00000101347.7583.82930782.50020-0.422504.221003.54590K562AGGF1
SAMHD1ENSG00000101347.7643.74780859.80730-0.417503.321502.22300K562AKAP8
SAMHD1ENSG00000101347.7475.57720909.17610-0.934907.078506.21060K562AQR
SAMHD1ENSG00000101347.7753.30210935.16420-0.312002.533501.41550K562CCDC124
SAMHD1ENSG00000101347.7647.94300796.54950-0.297902.307301.30650K562CPEB4
SAMHD1ENSG00000101347.71069.14660824.425800.374904.347903.55680K562DDX47
SAMHD1ENSG00000101347.7339.53870483.11490-0.508803.086202.11910K562EFTUD2
SAMHD1ENSG00000101347.7616.84880446.200100.467103.837202.90960K562EIF3G
SAMHD1ENSG00000101347.7264.71730479.02610-0.855708.254206.83740K562EIF4A3
SAMHD1ENSG00000101347.7326.18350431.36390-0.403202.733701.59020K562EIF4B
SAMHD1ENSG00000101347.7481.98590745.09390-0.628404.831303.68850K562FIP1L1
SAMHD1ENSG00000101347.7676.57910498.162500.441503.838703.14330K562FXR1
SAMHD1ENSG00000101347.7448.56160592.67950-0.401902.799701.72190K562HNRNPA1
SAMHD1ENSG00000101347.7868.16110656.402800.403303.943402.87130K562HNRNPU
SAMHD1ENSG00000101347.7291.82500425.39870-0.543702.510501.62420K562KRR1
SAMHD1ENSG00000101347.7397.07830552.92180-0.477703.499602.54870K562MAGOH
SAMHD1ENSG00000101347.7899.760501166.03910-0.374002.479101.41200K562MSI2
SAMHD1ENSG00000101347.7484.89760656.35030-0.436804.092203.12460K562MTPAP
SAMHD1ENSG00000101347.7655.59630509.423000.363802.558601.79100K562POLR2G
SAMHD1ENSG00000101347.7817.238701193.57010-0.546504.596603.54930K562PUF60
SAMHD1ENSG00000101347.7668.67280453.975700.558604.734303.31120K562SAFB
SAMHD1ENSG00000101347.7298.33540542.73100-0.863303.113701.81390K562SF1
SAMHD1ENSG00000101347.7537.97380719.57140-0.419603.845002.89200K562SF3A3
SAMHD1ENSG00000101347.7218.54670451.58800-1.0471012.3564010.47260K562SF3B4
SAMHD1ENSG00000101347.7450.18990737.20950-0.7115010.794109.08890K562SRPK2
SAMHD1ENSG00000101347.7298.36030455.24610-0.609603.569702.40030K562SUPV3L1
SAMHD1ENSG00000101347.7787.78310510.994300.624404.942103.83770K562SYNCRIP
SAMHD1ENSG00000101347.7258.84930532.98440-1.0420011.343209.75470K562TARDBP
SAMHD1ENSG00000101347.7285.57430420.95940-0.559803.129901.95470K562U2AF1
SAMHD1ENSG00000101347.7286.25140434.78110-0.603004.787403.73400K562U2AF2
SAMHD1ENSG00000101347.7626.33860773.30530-0.304102.644101.94170K562UPF1
SAMHD1ENSG00000101347.7498.67940661.69970-0.408103.061102.14230K562WDR3
SAMHD1ENSG00000101347.7661.213701076.90210-0.703705.698203.84690K562WRN
SAMHD1ENSG00000101347.7637.155201024.36670-0.685004.958303.74890K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for SAMHD1 is not available on ENCORE database.





3 RBPs can affect the splicing of SAMHD1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SESAMHD1ENSG00000101347.7chr20-3556374335563806355634313556359235569441355695140.054000.002730.06654includedHepG2SF3A3
A3SSSAMHD1ENSG00000101347.7chr20-3554535135545499355453513554545235547766355479220.254000.000020.00723longerHepG2TAF15
A3SSSAMHD1ENSG00000101347.7chr20-3554535135545499355453513554545235547766355479220.154000.010680.05590longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for SAMHD1 is not avaliable on ENCORE database.