RPS28

General Information

Gene Name       : RPS28
Gene ID             : ENSG00000233927.4
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RPS28)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RPS28ENSG00000233927.446.57442.97244.77ENCSR000AEF
HepG2RPS28ENSG00000233927.41314.19223.56768.88ENCSR985KAT
K562RPS28ENSG00000233927.4763.52582.50673.01ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RPS28-BGKcLV33-K562RPS28K562Released   CRISPRKD% = 12.2,18.2
knockdown followed by RNA-seq RPS28-BGKcLV33-K562ENCSR928XFZRPS28K562ReleasedENCSR271ZAB   CRISPRKD% = 0.0,0.0






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RPS28ENCAB557DQGA305-095A1BGKcLV33-13, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RPS28K562CRISPRBGKcLV33-5,BGKcLV33-6BGKcLV33-1,BGKcLV33-212.2,18.2ReleasedENCODE4
RPS28K562CRISPRENCSR928XFZENCSR271ZABRPS28-BGKcLV33-13,RPS28-BGKcLV33-14NT-BGKcLV33-1,NT-BGKcLV33-20.0,0.0ReleasedENCODE4
RPS28HepG2CRISPRBGHcLV31-7,BGHcLV31-8BGHcLV31-1,BGHcLV31-20.0,0.0NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS28Product_ID: A305-095A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPS28-human		 RPS28-K562-CRISPR-A305-095A.png<br>Caption: Western blot following CRISPR against RPS28 in K562 whole cell lysate using RPS28 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS28. RPS28 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RPS28 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RPS28 RNA is bound by 18 proteins.  







eCLIP assay for RPS28 is not avaliable on ENCORE database.





18 proteins are bound to RPS28 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1983871558387179+3'-UTR,intron-Proximal3.605277.91814HepG2DDX3X
chr1983871558387175+3'-UTR,intron-Proximal3.6233713.02469HepG2LARP4
chr1983871558387175+3'-UTR,intron-Proximal3.1991618.49410K562LARP4
chr1983872218387257+3'-UTR4.638224.61933HepG2LARP4
chr1983871558387175+3'-UTR,intron-Proximal3.3063041.54532K562PABPC4
chr1983871468387177+3'-UTR,intron-Proximal4.369554.52312K562AQR
chr1983871548387173+3'-UTR,intron-Proximal4.5441410.82619HepG2RPS3
chr1983872548387274+3'-UTR5.1781219.51834K562TIA1
chr1983872228387231+3'-UTR3.9675514.32877K562TIA1
chr1983871548387207+3'-UTR,intron-Proximal3.418717.02421K562METAP2
chr1983872348387262+3'-UTR4.069808.41415K562PABPC4
chr1983872318387254+3'-UTR5.2803321.85189K562TIA1
chr1983869198386976+5'-UTR,CDS,intron-Proximal3.196327.88981K562METAP2
chr1983869598387012+5'-UTR,intron-Proximal4.378834.59919HepG2AQR
chr1983869488387016+5'-UTR,CDS,intron-Proximal3.4976010.87706HepG2PRPF8
chr1983869288386978+5'-UTR,CDS,intron-Proximal3.092865.55505K562DDX24
chr1983864028386427+5'-UTR,CDS3.100595.27722HepG2DDX55
chr1983869478386975+5'-UTR,CDS3.2848549.94950K562PABPC4
chr1983869498386979+5'-UTR,CDS,intron-Proximal3.792479.13812HepG2DDX3X
chr1983869658386976+5'-UTR,intron-Proximal3.7337214.04580HepG2LARP4
chr1983869488386977+5'-UTR,CDS,intron-Proximal4.2447610.69327HepG2RPS3
chr1983869198386965+5'-UTR,CDS3.8064116.74105HepG2LARP4
chr1983868358386857+CDS,intron-Proximal3.412068.24214HepG2LARP4
chr1983865578386583+CDS3.5587761.38149K562DDX3X
chr1983864268386450+CDS3.4182016.54648HepG2LARP4
chr1983867838386842+CDS,intron-Proximal3.962115.35619K562AQR
chr1983864348386453+CDS3.2863733.69336K562PABPC4
chr1983865848386653+CDS,intron-Proximal3.2635512.30918HepG2BUD13
chr1983865558386563+CDS3.31312400.00000K562YBX3
chr1983868368386856+CDS,intron-Proximal3.5552446.30637K562DDX3X
chr1983864508386453+CDS3.5441814.37337HepG2LARP4
chr1983864898386570+CDS,intron-Proximal3.867438.33266K562SF3B4
chr1983865398386555+CDS,intron-Proximal3.32714400.00000K562YBX3
chr1983868868386906+CDS3.2451710.95593K562YBX3
chr1983865688386588+CDS,intron-Proximal3.446178.46777HepG2LARP4
chr1983865508386588+CDS,intron-Proximal3.323974.21265HepG2DDX3X
chr1983865858386690+CDS,intron-Distal,intron-Proximal4.1235712.68495HepG2EFTUD2
chr1983864508386453+CDS3.49113400.00000K562YBX3
chr1983865838386588+CDS,intron-Proximal3.9258849.53567K562DDX3X
chr1983864358386443+CDS3.54057400.00000K562YBX3
chr1983864438386450+CDS3.46256400.00000K562YBX3
chr1983867618386846+CDS,intron-Proximal3.406476.76873HepG2AQR
chr1983865868386594+CDS,intron-Proximal4.3841012.08528K562AQR
chr1983868368386854+CDS,intron-Proximal4.2991667.72941K562PABPC4
chr1983864918386584+CDS,intron-Proximal3.1069818.18121HepG2BUD13
chr1983865398386568+CDS,intron-Proximal4.0461823.13152HepG2LARP4
chr1983865798386587+CDS3.9117212.19227K562APOBEC3C
chr1983865398386553+CDS,intron-Proximal3.1892625.89815K562PABPC4
chr1983867748386841+CDS,intron-Proximal4.7297314.03953HepG2SF3B4
chr1983868348386846+CDS,intron-Proximal3.236227.43702K562GEMIN5
chr1983864368386454+CDS,intron-Proximal5.2923214.42751K562EIF3G
chr1983865578386588+CDS,intron-Proximal3.98846400.00000K562PABPC4
chr1983865398386546+CDS,intron-Proximal3.007785.05890K562METAP2
chr1983865628386631+CDS,intron-Proximal3.3998320.60281HepG2PRPF8
chr1983867868386851+CDS,intron-Proximal3.500647.98670K562SF3B4
chr1983864278386434+CDS3.3531612.19960K562PABPC4
chr1983868368386850+CDS,intron-Proximal3.124253.67794HepG2DDX3X
chr1983865398386577+CDS,intron-Proximal4.1788510.68912K562EIF3G
chr1983865738386589+CDS,intron-Proximal3.398708.81638K562GEMIN5
chr1983865488386573+CDS3.1658319.14514K562GEMIN5
chr1983868368386853+CDS,intron-Proximal3.6076110.21081K562APOBEC3C
chr1983871058387153+intron-Proximal4.541635.63851K562SF3B4
chr1983864848386537+intron-Proximal3.227773.13970HepG2SF3B4
chr1983865948386619+intron-Proximal3.9759014.06167K562AQR
chr1983870168387069+intron-Proximal3.552153.99381K562BUD13
chr1983864868386536+intron-Proximal4.656368.04968HepG2EFTUD2
chr1983870148387096+intron-Proximal3.486904.50953K562CSTF2T
chr1983866198386632+intron-Proximal4.2053814.21033K562AQR
chr1983866348386683+intron-Proximal3.615787.07647K562PRPF8
chr1983865998386630+intron-Proximal3.158643.01994K562BUD13
chr1983870798387141+intron-Proximal3.476435.85035HepG2SF3B4
chr1983870228387069+intron-Proximal4.751868.66247K562EFTUD2
chr1983870298387109+intron-Proximal4.8661711.88533K562PRPF8
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RPS28 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RPS28 is not available on ENCORE database.

  • 106 RBPs can affect the levels of RPS28.  








The Knockdown/knockout RNA-Seq assay for RPS28 is not available on ENCORE database.





106 RBPs can affect the gene expression of RPS28 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RPS28ENSG00000233927.41770.434802223.41120-0.328702.260601.70050HepG2ACO1
RPS28ENSG00000233927.4630.35640302.548301.058903.619302.49700HepG2ADAR
RPS28ENSG00000233927.43404.994604602.94960-0.434903.157902.43090HepG2AUH
RPS28ENSG00000233927.4642.71300493.612400.380702.285501.73590HepG2BOP1
RPS28ENSG00000233927.4445.85640994.71930-1.157707.162905.25510HepG2BUD13
RPS28ENSG00000233927.4642.65770453.145200.504002.355001.71330HepG2CPSF6
RPS28ENSG00000233927.4660.01810310.270001.0889017.8148015.41910HepG2YBX3
RPS28ENSG00000233927.41419.60710930.128000.609903.508802.80290HepG2CELF1
RPS28ENSG00000233927.41775.301701000.048400.8279027.7333025.85160HepG2DAZAP1
RPS28ENSG00000233927.42688.179302056.208100.386508.337607.02640HepG2DDX19B
RPS28ENSG00000233927.42468.790402127.129600.214802.481701.83440HepG2DKC1
RPS28ENSG00000233927.4545.39380304.633300.840105.410404.45620HepG2DNAJC21
RPS28ENSG00000233927.4642.97210301.984901.0902022.1289020.44560HepG2DNAJC2
RPS28ENSG00000233927.42426.42610945.153701.3601013.9896012.25970HepG2EIF4G2
RPS28ENSG00000233927.43707.749605285.34120-0.511504.295703.57490HepG2ETF1
RPS28ENSG00000233927.41100.972401689.13150-0.617508.555307.21420HepG2FUBP3
RPS28ENSG00000233927.4327.53080513.62630-0.649104.232703.45220HepG2GPKOW
RPS28ENSG00000233927.41420.77510836.114300.7648016.8582015.28290HepG2HNRNPF
RPS28ENSG00000233927.42209.71070910.947101.2783068.6763066.04190HepG2HNRNPL
RPS28ENSG00000233927.4677.80160339.846000.995905.307604.48890HepG2HNRNPU
RPS28ENSG00000233927.41303.00850823.329300.662203.400702.60420HepG2IGF2BP1
RPS28ENSG00000233927.4708.83880347.139001.0298020.2069018.43920HepG2ILF2
RPS28ENSG00000233927.42430.986202032.302400.258304.229902.98140HepG2KIF1C
RPS28ENSG00000233927.41788.10510991.649600.8504016.9231015.13080HepG2NOL12
RPS28ENSG00000233927.4596.28300344.399100.791804.970103.07510HepG2NOLC1
RPS28ENSG00000233927.4576.90390336.372000.778203.826902.84860HepG2PARN
RPS28ENSG00000233927.41337.617203775.75280-1.497107.462906.54460HepG2PCBP1
RPS28ENSG00000233927.4757.13380303.113001.3206011.4609010.01890HepG2PPIL4
RPS28ENSG00000233927.41802.520803842.53290-1.092004.701204.03010HepG2PTBP1
RPS28ENSG00000233927.41538.57540946.635000.7006010.386409.33050HepG2PUF60
RPS28ENSG00000233927.42146.755201004.916701.0950053.1495051.23760HepG2PUM1
RPS28ENSG00000233927.41690.676901004.169100.751504.686903.82530HepG2PUM2
RPS28ENSG00000233927.41553.264302264.64760-0.5440012.0912010.96000HepG2PUS1
RPS28ENSG00000233927.42458.524304160.35740-0.758902.512301.91200HepG2QKI
RPS28ENSG00000233927.42128.701303776.24540-0.827002.979602.28350HepG2RBM25
RPS28ENSG00000233927.41837.28070924.332400.991003.238602.16100HepG2RBM27
RPS28ENSG00000233927.42325.980004275.90220-0.878403.590502.99060HepG2NELFE
RPS28ENSG00000233927.41080.07660302.639701.8354037.6404035.68900HepG2SAFB2
RPS28ENSG00000233927.41794.330103999.56260-1.156405.366504.58910HepG2SF3B4
RPS28ENSG00000233927.42010.07880907.720301.1468042.4463040.39960HepG2SLBP
RPS28ENSG00000233927.4610.40340352.915300.7903011.6842010.50570HepG2SMN1
RPS28ENSG00000233927.43566.879604989.15390-0.484103.762203.20520HepG2SRFBP1
RPS28ENSG00000233927.4563.428604175.08980-2.8895016.6455015.69270HepG2SRSF1
RPS28ENSG00000233927.41512.50580934.690400.694304.766203.98170HepG2SRSF5
RPS28ENSG00000233927.41170.68070821.470700.511002.233901.60180HepG2SRSF7
RPS28ENSG00000233927.41072.843403397.01300-1.662808.163407.30530HepG2SRSF9
RPS28ENSG00000233927.43164.188204331.41980-0.453003.740303.16420HepG2SSB
RPS28ENSG00000233927.41579.872901062.978000.5716012.7416011.57070HepG2SUCLG1
RPS28ENSG00000233927.4612.916903753.87760-2.6146013.0812011.99820HepG2TAF15
RPS28ENSG00000233927.4637.89550477.891600.416502.487101.79400HepG2TARDBP
RPS28ENSG00000233927.4960.772903448.08810-1.843508.636007.17070HepG2TIA1
RPS28ENSG00000233927.41003.05860333.179201.5899050.6852048.67250HepG2TRIM56
RPS28ENSG00000233927.4717.080201146.97120-0.677603.383102.69380HepG2U2AF1
RPS28ENSG00000233927.4324.48320737.90630-1.185303.771602.61000HepG2U2AF2
RPS28ENSG00000233927.41422.99190978.971100.539504.508303.42160HepG2UBE2L3
RPS28ENSG00000233927.4851.70810475.087000.842103.227102.40890HepG2UCHL5
RPS28ENSG00000233927.41131.477601350.31360-0.255102.281301.31640HepG2UTP18
RPS28ENSG00000233927.41462.68740791.932600.885102.775202.04910HepG2XRN2
RPS28ENSG00000233927.41075.241301444.85150-0.426307.778106.98200HepG2ZNF622
RPS28ENSG00000233927.49057.630705088.336600.8318019.0932017.72510K562AGGF1
RPS28ENSG00000233927.48304.115305591.869500.570402.247301.37290K562AKAP8
RPS28ENSG00000233927.49947.563705294.781900.9097010.661209.26870K562AKAP8L
RPS28ENSG00000233927.412960.703708433.578400.619802.650902.07330K562AQR
RPS28ENSG00000233927.47667.172505555.759600.464603.571302.52160K562ATP5C1
RPS28ENSG00000233927.43872.771402300.082600.751606.610305.09620K562BCLAF1
RPS28ENSG00000233927.43463.033902211.803100.646707.385305.39550K562CIRBP
RPS28ENSG00000233927.44440.839103371.860300.397203.183101.61810K562CKAP4
RPS28ENSG00000233927.44729.720003944.947800.261603.319802.09950K562CPEB4
RPS28ENSG00000233927.43070.906601744.956900.815408.198606.25040K562CSTF2
RPS28ENSG00000233927.43005.677902015.884100.576203.279201.82320K562DDX27
RPS28ENSG00000233927.42944.720404081.48240-0.4710011.083309.87630K562DDX47
RPS28ENSG00000233927.43247.925001756.331500.886902.498101.63360K562EFTUD2
RPS28ENSG00000233927.45927.893904777.783100.311101.989201.36160K562EIF2S2
RPS28ENSG00000233927.41059.460501781.75720-0.750005.159403.74620K562EIF3A
RPS28ENSG00000233927.41210.85760822.259000.558307.231205.95610K562EIF3G
RPS28ENSG00000233927.48812.180105571.717600.661304.597102.06000K562EIF3H
RPS28ENSG00000233927.43339.876901979.632600.7545013.2379011.23420K562FIP1L1
RPS28ENSG00000233927.41570.797302128.20910-0.438104.259503.19890K562FMR1
RPS28ENSG00000233927.42928.682204176.39260-0.512003.514302.84440K562FXR1
RPS28ENSG00000233927.468.81530513.22280-2.898809.875708.38300K562G3BP2
RPS28ENSG00000233927.410365.669506365.840200.703304.399903.45170K562GRSF1
RPS28ENSG00000233927.45535.781303693.617600.583702.821501.58920K562HNRNPA2B1
RPS28ENSG00000233927.44091.190102683.753300.608209.086307.66060K562HSPD1
RPS28ENSG00000233927.45985.882102065.163401.535202.764501.73820K562IGF2BP2
RPS28ENSG00000233927.44009.791102099.949700.933106.870404.86850K562IGF2BP3
RPS28ENSG00000233927.42020.244603236.64910-0.680004.311902.52060K562ILF2
RPS28ENSG00000233927.48126.244105618.606900.532305.623104.35340K562KIF1C
RPS28ENSG00000233927.43555.114502168.918700.712804.397803.33310K562MAGOH
RPS28ENSG00000233927.47348.868805378.778700.450104.938903.37120K562MAK16
RPS28ENSG00000233927.43211.280302164.292500.569203.031702.06310K562MATR3
RPS28ENSG00000233927.43914.029101734.591601.174003.845702.25910K562NONO
RPS28ENSG00000233927.45263.164004227.002600.3162010.015408.71630K562NSUN2
RPS28ENSG00000233927.42120.257503405.67520-0.683704.758903.10530K562PABPC1
RPS28ENSG00000233927.46228.480303231.980100.946406.364204.67670K562PABPN1
RPS28ENSG00000233927.43279.663302120.813300.628803.045801.62530K562PCBP1
RPS28ENSG00000233927.42560.264901929.529100.407903.710602.57910K562PCBP2
RPS28ENSG00000233927.42790.980904597.54450-0.720102.020101.31040K562PCBP3
RPS28ENSG00000233927.42492.261403526.37530-0.500707.102205.83130K562POLR2G
RPS28ENSG00000233927.44216.924002247.488600.907807.279605.82450K562PPIG
RPS28ENSG00000233927.46308.529203843.556400.714803.446801.75540K562PPP1R8
RPS28ENSG00000233927.410338.498005249.991700.9775012.6031011.48980K562PUS1
RPS28ENSG00000233927.48920.755205116.137400.8020012.9356011.65170K562RBM15
RPS28ENSG00000233927.42645.665102125.797000.315502.420001.40930K562NELFE
RPS28ENSG00000233927.41767.975103162.44010-0.838907.300204.63710K562RPS3A
RPS28ENSG00000233927.42456.708203248.73320-0.403204.218902.39860K562SDAD1
RPS28ENSG00000233927.44908.221504170.996700.234702.924502.08870K562SF3A3
RPS28ENSG00000233927.41107.19400532.911001.054807.779906.30460K562SF3B4
RPS28ENSG00000233927.43216.745702066.073900.638602.089401.46490K562SRSF1
RPS28ENSG00000233927.43675.148602435.273700.593607.745005.85720K562SRSF4
RPS28ENSG00000233927.44701.444901951.664301.268309.935307.79870K562SRSF5
RPS28ENSG00000233927.43205.033704013.75200-0.324604.184802.29730K562SRSF9
RPS28ENSG00000233927.42677.202403537.18190-0.401905.688804.50310K562SYNCRIP
RPS28ENSG00000233927.45314.296802090.641901.3458013.8109012.00110K562TARDBP
RPS28ENSG00000233927.44938.287702016.754701.291903.798702.81720K562TIA1
RPS28ENSG00000233927.44927.137301979.887601.3152019.7697016.17960K562TRA2A
RPS28ENSG00000233927.43016.483502314.952100.381803.057901.58800K562TRIP6
RPS28ENSG00000233927.4263.70490513.19270-0.960603.039602.19790K562U2AF2
RPS28ENSG00000233927.43044.092403988.06090-0.389709.598208.12800K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RPS28 is not available on ENCORE database.





5 RBPs can affect the splicing of RPS28 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
RIRPS28ENSG00000233927.4chr19+838652583867038386525838658783866678386703-0.115000.010580.05650excludedK562AQR
MXERPS28ENSG00000233927.4chr19+838653983865878386836838697583863838386453838715583882240.073000.000020.003311includedK562TRA2A
MXERPS28ENSG00000233927.4chr19+838653983865878386836838697583863838386453838715583882240.085000.001160.078201includedK562CPSF6
MXERPS28ENSG00000233927.4chr19+838653983865878386836838697583863838386453838715583882240.078000.001920.032181includedK562SF3B4
MXERPS28ENSG00000233927.4chr19+838653983865878386836838697583863838386453838715583882240.066000.000180.006721includedK562SUPV3L1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RPS28 is not avaliable on ENCORE database.