RPS28      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-95BGHcLV49-96
idx00
TRCN#_or_BGC#BGC#0000823BGC#0000823
shRNA_or_gRNA_sequenceTTCGGGCCCCCACCTCACCCTTCGGGCCCCCACCTCACCC
PAMTGGTGG
NameRPS28_55RPS28_55
Sample_IDBGHcLV49-95BGHcLV49-96
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameRPS28RPS28
qPCR_result50.348.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc30043597
WB_result52.254.0
Ave_WB
WB_DONE_date7/1/25
MW7.8kd
IP
antibody_Cat#A305-095ALOT #1
Antibody DCC IDENCAB557DQG
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99819982




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS28Product_ID: A305-095A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPS28-human		 RPS28-hepg2-CRISPR-A305-095A-LICOR.png<br>Caption: Western blot following CRISPR against RPS28 in HepG2 whole cell lysate using RPS28 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS28. RPS28 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 RPS28-K562-CRISPR-A305-095A.png<br>Caption: Western blot following CRISPR against RPS28 in K562 whole cell lysate using RPS28 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS28. RPS28 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-95BGHcLV49-96
Sample_IDBGHcLV49-95BGHcLV49-96
Sample Name
Sample NameRPS28-BGHcLV49-95RPS28-BGHcLV49-96
RBPRPS28RPS28
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1436214363




Library-Prep Information
BGHcLV49-95BGHcLV49-96
Sample #5960
Sample NameRPS28-BGHcLV49-95RPS28-BGHcLV49-96
Sample_Name_Alias
Index Well PositionE08F08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp264260
Peak_Molarity71.2075.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-95BGHcLV49-96
RBPRPS28RPS28
Batch_IDBGHcLV49BGHcLV49
WB_result52.20054.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48044805




Sequencing Information
BGHcLV49-95BGHcLV49-96
Sample_IDBGHcLV49-95BGHcLV49-96
Sample NameRPS28-BGHcLV49-95RPS28-BGHcLV49-96
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35293530




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database