NFX1

General Information

Gene Name       : NFX1
Gene ID             : ENSG00000086102.18
Essential Gene : No

Functions

Localization(RBPImage: NFX1)

Domains (AlphaFold: NFX1)


Antibody Validation

 View validation images for all NFX1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878NFX1ENSG00000086102.1829.0130.2329.62ENCSR000AEF
HepG2NFX1ENSG00000086102.1819.7124.4522.08ENCSR985KAT
K562NFX1ENSG00000086102.1823.6625.8924.77ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq NFX1-BGKLV13-K562ENCSR007XKLNFX1K562ReleasedENCSR815CVQ   shRNAKD% = ,
knockdown followed by RNA-seq NFX1-BGHcLV10-HepG2ENCSR548ORVNFX1HepG2ReleasedENCSR775ZWO   CRISPRKD% = 65.9,60.7






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
NFX1567K562ENCSR276LGLENCSR821ESGENCAB841CSSA302-914A1RevokedENCODE3


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
NFX1ENCAB841CSSA302-914A1Released, ENCODE3BGHcLV10-29, Released, ENCODE4
NFX1A302-916A1BGHcLV10-29, Released, ENCODE4BGKcLV44-35, Submitting, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
NFX1K562shRNAENCSR007XKLENCSR815CVQNFX1_BGKLV13_25,NFX1_BGKLV13_26NT_BGKLV13_1,NT_BGKLV13_2,ReleasedENCODE3
NFX1K562shRNABGKLV28-29,BGKLV28-30BGKLV28-1,BGKLV28-29.6,39.9NotSatisfiedENCODE3
NFX1HepG2shRNABGHLV23-23,BGHLV23-24BGHLV23-1,BGHLV23-2redo transfection,redo transfectionNotSatisfiedENCODE3
NFX1HepG2shRNABGHLV26-25,BGHLV26-26BGHLV26-1,BGHLV26-225.0,12.7NotSatisfiedENCODE3
NFX1K562CRISPRBGKcLV02-13,BGKcLV02-14BGKcLV02-1,BGKcLV02-20.0,0.0NotSatisfiedENCODE3
NFX1K562CRISPRBGKcLV08-41,BGKcLV08-42BGKcLV08-1,BGKcLV08-214.5,1.3NotSatisfiedENCODE4
NFX1K562CRISPRNFX1-BGKcLV44-35,NFX1-BGKcLV44-36NT-BGKcLV44-1,NT-BGKcLV44-261.6,61.9SequencedENCORE2
NFX1HepG2CRISPRBGHcLV03-11,BGHcLV03-12BGHcLV03-1,BGHcLV03-20.0,0.0NotSatisfiedENCODE3
NFX1HepG2CRISPRENCSR548ORVENCSR775ZWONFX1-BGHcLV10-29,NFX1-BGHcLV10-30NT-BGHcLV10-1,NT-BGHcLV10-265.9,60.7ReleasedENCODE4
NFX1HepG2CRISPRBGHcLV10-31,BGHcLV10-32BGHcLV10-1,BGHcLV10-2NotSatisfiedENCODE4
NFX1HepG2CRISPRBGHcLV45-45,BGHcLV45-46BGHcLV45-1,BGHcLV45-2NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    NFX1: Click to RBP Image Database
  • Nuclei
  • Nucleoplasm
  • Mutually Exclusive from Nucleolus
  • Cytoplasm
  • Compartment/Organelle
  • Unknown Cyto Foci
  • Cytosolic
  • Cell Cortex












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    NFX1Product_ID: A302-914A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: NFX1-human    		 NFX1-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against NFX1 in HepG2 whole cell lysate using NFX1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NFX1.NFX1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
    		 K562_Bethyl_A302-914A_1_NFX1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NFX1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NFX1 antibody (lanes under 'NFX1').
    NFX1Product_ID: A302-916A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: NFX1-human    		 NFX1-K562-CRISPR-A302-916A.png<br>Caption: Western blot following CRISPR against NFX1 in K562 whole cell lysate using NFX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NFX1. NFX1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








    RNA Targets

    • eCLIP assay for NFX1 is not avaliable on ENCORE database.



    Proteins bound to RNA
    • NFX1 RNA is bound by 17 proteins.  







    eCLIP assay for NFX1 is not avaliable on ENCORE database.





    17 proteins are bound to NFX1 RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr93334804133348117+3'-UTR,intron-Distal3.786373.53569K562LIN28B
    chr93329056433290650+5'-UTR,CDS,intron-Proximal3.801744.83899HepG2PRPF8
    chr93329053833290611+5'-UTR,CDS,intron-Proximal3.132915.27008HepG2NCBP2
    chr93329535133295420+CDS4.549089.47159HepG2BCLAF1
    chr93336666833366725+CDS4.515935.27053HepG2PPIG
    chr93331878533318828+CDS5.337628.40988HepG2BCLAF1
    chr93331365033313696+CDS,intron-Proximal3.985236.39364HepG2PPIG
    chr93329501433295118+CDS3.683585.30520K562UCHL5
    chr93331891133318979+CDS3.327456.86395HepG2BCLAF1
    chr93329482733294993+CDS4.1377517.19016HepG2BCLAF1
    chr93331377833313831+CDS,intron-Proximal3.746589.70941HepG2PRPF8
    chr93333849033338539+CDS,intron-Proximal3.812535.18845HepG2U2AF2
    chr93330318333303262+CDS,intron-Proximal3.814328.12130HepG2PPIG
    chr93334279533342842+CDS4.872826.37937HepG2BCLAF1
    chr93330727233307421+CDS,intron-Distal,intron-Proximal3.997355.84589K562EFTUD2
    chr93333855333338555+CDS4.430563.71570HepG2BCLAF1
    chr93330719133307242+CDS,intron-Proximal3.723455.40651HepG2PPIG
    chr93330133133301413+CDS4.520765.13243HepG2GRWD1
    chr93331378833313810+CDS,intron-Proximal4.955114.09264K562AQR
    chr93330725133307289+CDS4.826266.13315HepG2BCLAF1
    chr93329438233294457+CDS,intron-Proximal4.618724.92499HepG2AQR
    chr93331372133313791+CDS3.488706.51082HepG2PPIG
    chr93336474233364803+CDS,intron-Proximal3.928103.01428K562UCHL5
    chr93331111033311176+CDS,intron-Proximal3.723457.69921HepG2PPIG
    chr93329506433295132+CDS4.198865.79432HepG2BCLAF1
    chr93329472133294827+CDS3.411828.50351HepG2BCLAF1
    chr93329526233295329+CDS4.104863.29312HepG2FXR2
    chr93330136133301420+CDS,intron-Proximal4.2115010.57092HepG2BCLAF1
    chr93329439733294483+CDS,intron-Proximal3.408864.57751HepG2U2AF2
    chr93331371033313766+CDS3.090125.38711K562YBX3
    chr93330718933307238+CDS,intron-Proximal3.322825.79905HepG2BCLAF1
    chr93330322233303265+CDS4.450118.41475HepG2BCLAF1
    chr93329214133292201+intron-Distal4.101503.79098HepG2SUGP2
    chr93329340033293524+intron-Distal3.964703.06150K562KHSRP
    chr93329296433293014+intron-Distal3.394453.59523HepG2SUGP2
    chr93330443933304522+intron-Distal5.084126.58773K562RBFOX2
    chr93336606333366153+intron-Distal,intron-Proximal5.758308.60492HepG2PCBP2
    chr93331384733313912+intron-Distal,intron-Proximal4.356863.49003K562PRPF8
    chr93329277433292857+intron-Distal4.701826.08961HepG2PTBP1
    chr93335920033359268+intron-Distal3.909343.09122HepG2PTBP1
    chr93329370233293775+intron-Distal5.798948.38577HepG2PRPF8
    chr93329264333292704+intron-Distal4.422024.61447HepG2SUGP2
    chr93331501433315072+intron-Distal3.610343.32861HepG2U2AF2
    chr93331381033313843+intron-Proximal5.066314.86944K562AQR
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for NFX1 is not avaliable on ENCORE database.



    Diffrential Expression
    • 49 differentially expressed genes after Knockdown/Knockout NFX1 protein.  





    • 50 RBPs can affect the levels of NFX1.  







    49 differentially expressed genes after Knockdown/Knockout NFX1 protein :

    Only display 100 entries as example. Please click "View All" button to see all 49 entries.     

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    ALOX5ENSG00000012779.6150.8664066.732901.176704.374501.66300K562NFX1
    WWC3ENSG00000047644.13194.9162075.865101.361206.272203.18630K562NFX1
    SLC2A3ENSG00000059804.112692.785404838.73240-0.845505.186702.25340K562NFX1
    CD84ENSG00000066294.10186.3535064.991601.519607.338704.05320K562NFX1
    ISOC1ENSG00000066583.7271.62530726.66730-1.419706.632803.47230K562NFX1
    NFX1ENSG00000086102.14530.565001062.99220-1.002506.217303.17480K562NFX1
    PGRMC1ENSG00000101856.8858.351101561.33960-0.863105.006202.13290K562NFX1
    RNF167ENSG00000108523.11342.82440829.92340-1.275509.104805.58530K562NFX1
    COL1A1ENSG00000108821.9164.7935047.916301.782008.617305.20690K562NFX1
    TREML2ENSG00000112195.841.39570107.10560-1.371504.535401.75060K562NFX1
    EPAS1ENSG00000116016.9241.45670111.146101.119205.047102.15960K562NFX1
    SGK1ENSG00000118515.7178.6466074.408701.263505.404302.45460K562NFX1
    EGR1ENSG00000120738.7687.546901930.89790-1.4897013.324809.65920K562NFX1
    TRIM6ENSG00000121236.15581.47120262.430101.147707.056903.80620K562NFX1
    CHST3ENSG00000122863.522.553701.990903.501804.470601.71030K562NFX1
    MT2AENSG00000125148.6388.96130124.318701.6455011.204307.61780K562NFX1
    HECTD3ENSG00000126107.10646.521601301.46660-1.009406.328803.21940K562NFX1
    RNASE1ENSG00000129538.9293.95170122.116401.267206.557003.42280K562NFX1
    APOEENSG00000130203.51044.90640469.490801.154107.900004.53540K562NFX1
    H19ENSG00000130600.11635.343101310.57550-1.044606.942103.72360K562NFX1
    CDK4ENSG00000135446.121226.171402121.60030-0.791004.440901.70980K562NFX1
    TUBB2BENSG00000137285.9724.46710332.895201.121804.241301.53940K562NFX1
    PTPRFENSG00000142949.12362.39190161.761001.163606.248303.18470K562NFX1
    TWF1ENSG00000151239.9771.44440419.272600.879604.741501.92090K562NFX1
    SEPN1ENSG00000162430.12494.94760912.89440-0.883204.065701.39120K562NFX1
    LAPTM5ENSG00000162511.71453.23350795.033400.870105.089302.17180K562NFX1
    CTSSENSG00000163131.6266.96850128.200801.058204.852802.00670K562NFX1
    SNHG16ENSG00000163597.101145.630502131.78170-0.895905.450302.48360K562NFX1
    YPEL4ENSG00000166793.686.4112030.820601.487204.507801.73430K562NFX1
    KIF7ENSG00000166813.10126.7314037.656501.750704.819701.98650K562NFX1
    NKIRAS2ENSG00000168256.13639.157001209.53160-0.920204.590201.79380K562NFX1
    TRAPPC1ENSG00000170043.7186.16190449.54660-1.271905.654602.65170K562NFX1
    ENC1ENSG00000171617.980.6329025.479301.661905.064602.16240K562NFX1
    SLC6A19ENSG00000174358.1123.6189080.68960-1.772405.461002.48360K562NFX1
    FAM46CENSG00000183508.4712.75980393.887300.855504.462201.71030K562NFX1
    OSBP2ENSG00000184792.11442.06940817.32900-0.886604.878302.01880K562NFX1
    IFITM1ENSG00000185885.112156.738307133.04700-1.7257015.4846011.72200K562NFX1
    KRT10ENSG00000186395.6692.566501184.74450-0.774604.081401.39120K562NFX1
    PRG2ENSG00000186652.5960.13270247.251701.9572019.4885015.42490K562NFX1
    RELNENSG00000189056.92426.930601440.970100.752004.071101.39120K562NFX1
    ZNF121ENSG00000197961.71770.017301018.450300.797304.441601.70980K562NFX1
    ZNF816-ZNF321PENSG00000213801.420.36700170.66130-3.0668018.9802015.09270K562NFX1
    CKMT1AENSG00000223572.587.74200244.17140-1.476607.733304.41000K562NFX1
    ZNF883ENSG00000228623.230.9963088.00130-1.505404.636801.82840K562NFX1
    FAM203BENSG00000230567.371.160800.0000099999.9999020.8558016.49110K562NFX1
    FAM138FENSG00000233630.4105.0233023.106602.184208.824505.36290K562NFX1
    TNFRSF6BENSG00000243509.413.881200.0000099999.999904.423101.70190K562NFX1
    GYPBENSG00000250361.2619.46940297.907701.056106.087103.06490K562NFX1
    RPL36A-HNRNPH2ENSG00000257529.121.506400.0000099999.999906.806703.61820K562NFX1
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    50 RBPs can affect the gene expression of NFX1 :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    NFX1ENSG00000086102.141218.227501518.26120-0.317603.837003.05360HepG2AUH
    NFX1ENSG00000086102.141025.860301266.16480-0.303602.107001.57950HepG2BOP1
    NFX1ENSG00000086102.14805.965001012.67950-0.329401.987601.32500HepG2CIRBP
    NFX1ENSG00000086102.14939.805101234.02690-0.392902.658902.04420HepG2DDX3X
    NFX1ENSG00000086102.14780.762601060.05040-0.441202.780302.16110HepG2DDX6
    NFX1ENSG00000086102.141176.90100887.980200.406305.036304.17170HepG2DKC1
    NFX1ENSG00000086102.14602.61380775.57770-0.364003.127302.44080HepG2DNAJC2
    NFX1ENSG00000086102.14748.051701019.05630-0.446003.143202.51280HepG2EIF2S1
    NFX1ENSG00000086102.141622.655301354.585800.260403.031102.16120HepG2EIF4G1
    NFX1ENSG00000086102.14925.707101317.71580-0.509403.973903.21810HepG2GPKOW
    NFX1ENSG00000086102.14847.791401257.51820-0.568805.344304.49900HepG2HNRNPLL
    NFX1ENSG00000086102.141111.713501361.35940-0.292302.461501.74460HepG2NOL12
    NFX1ENSG00000086102.14937.736501299.58500-0.470804.556103.81290HepG2PUF60
    NFX1ENSG00000086102.141168.82600945.374600.306003.524102.84760HepG2PUS1
    NFX1ENSG00000086102.141538.751601280.765600.264702.208501.43240HepG2RBM15
    NFX1ENSG00000086102.141704.147601325.700600.362202.953102.25060HepG2RPLP0
    NFX1ENSG00000086102.14859.23100677.692800.342302.458301.87520HepG2RPS19
    NFX1ENSG00000086102.141319.392801645.57690-0.318704.290403.70610HepG2SRFBP1
    NFX1ENSG00000086102.142011.975701428.772100.4937010.309309.44040HepG2SSB
    NFX1ENSG00000086102.141240.320201459.31520-0.234602.360301.77740HepG2SUCLG1
    NFX1ENSG00000086102.141067.56980848.039200.332002.923702.41250HepG2TIAL1
    NFX1ENSG00000086102.14683.96030855.71720-0.323203.053202.46780HepG2TRIM56
    NFX1ENSG00000086102.141402.478601027.181700.449204.181503.41660HepG2U2AF1
    NFX1ENSG00000086102.14904.016601156.22160-0.355004.385003.53950HepG2UPF1
    NFX1ENSG00000086102.141255.699901026.946600.290003.457402.71990HepG2ZC3H8
    NFX1ENSG00000086102.141379.861301089.229400.341102.802801.61340K562AARS
    NFX1ENSG00000086102.14864.696501296.21560-0.5840010.046509.05280K562AGGF1
    NFX1ENSG00000086102.141042.573601424.27230-0.450104.539203.20520K562AKAP8
    NFX1ENSG00000086102.141032.522501348.75320-0.385503.374302.65130K562AKAP8L
    NFX1ENSG00000086102.141758.935302155.49480-0.293302.472601.91140K562AQR
    NFX1ENSG00000086102.144182.836603408.508000.295203.893702.72690K562CELF1
    NFX1ENSG00000086102.143047.197502265.074300.427808.555707.49060K562DDX47
    NFX1ENSG00000086102.141469.870801868.28260-0.346004.390103.51210K562EIF2S2
    NFX1ENSG00000086102.141415.658101005.132500.494006.844705.60440K562EIF3G
    NFX1ENSG00000086102.141005.98820799.020000.332203.012001.79130K562EIF4B
    NFX1ENSG00000086102.141622.35890922.732400.8140016.3884015.02340K562FXR1
    NFX1ENSG00000086102.142024.764902489.78450-0.298302.663401.92080K562GRSF1
    NFX1ENSG00000086102.141514.579301165.445800.377902.777101.53950K562HNRNPK
    NFX1ENSG00000086102.141326.848001641.32050-0.306903.212802.25410K562HNRNPU
    NFX1ENSG00000086102.141990.407702564.33850-0.365503.404602.52250K562HSPD1
    NFX1ENSG00000086102.141133.281501431.42700-0.336902.590001.79460K562KIF1C
    NFX1ENSG00000086102.141053.148601370.17700-0.379703.275102.07860K562MAK16
    NFX1ENSG00000086102.141532.769601223.909200.324502.839801.90420K562MATR3
    NFX1ENSG00000086102.14530.565001062.99220-1.002506.217303.17480K562NFX1
    NFX1ENSG00000086102.14736.277101145.45140-0.637602.809701.81170K562PABPN1
    NFX1ENSG00000086102.14857.236501068.81970-0.318302.147201.35960K562PSIP1
    NFX1ENSG00000086102.14913.022401337.72120-0.551105.499004.76550K562PUS1
    NFX1ENSG00000086102.141011.925301303.30160-0.365103.895803.20920K562RBM15
    NFX1ENSG00000086102.141238.571801013.235500.289602.432701.63310K562SF3B4
    NFX1ENSG00000086102.141905.369801500.079900.344903.767602.80920K562SYNCRIP
    NFX1ENSG00000086102.141302.78030975.515300.417304.515703.49540K562U2AF2
    NFX1ENSG00000086102.14987.251501248.40000-0.338602.248901.33940K562UCHL5
    NFX1ENSG00000086102.141821.288602428.40960-0.415104.556303.40720K562ZC3H8
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for NFX1 is not available on ENCORE database.





    2 RBPs can affect the splicing of NFX1 :

    Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
    SENFX1ENSG00000086102.14chr9+3329141833291576332905083329059533294417332954250.138000.000050.00360includedHepG2MATR3
    A3SSNFX1ENSG00000086102.14chr9+3331108433311175333111033331117533307191333072970.068000.000300.00237longerK562AQR

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    The Knockdown/knockout RNA-Seq assay for NFX1 is not avaliable on ENCORE database.