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NFX1 RNA-Seq in K562



NFX1      CRISPR in K562      Control:

General Information
RBPNFX1
Cell_LineK562
MethodCRISPR
Exp_NameNFX1-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1NFX1-BGKcLV44-35
Rep2NFX1-BGKcLV44-36
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR66.8
Rep2_qPCR66.4
Rep1_WB61.6
Rep2_WB61.9
Antibody Cat#A302-916A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1601




Experiment Information (Status: Sequenced)
BGKcLV44-35BGKcLV44-36
idx00
TRCN#_or_BGC#BGC#0001221BGC#0001221
shRNA_or_gRNA_sequenceTTCGTGTCACGGCCCCAGTGTTCGTGTCACGGCCCCAGTG
PAMTGGTGG
NameNFX1_81NFX1_81
Sample_IDBGKcLV44-35BGKcLV44-36
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameNFX1NFX1
qPCR_result66.866.4
Ave_qPCR66.6
RT-qPCR_primer-Fagaatgtggaaacgcacaca
RT-qPCR_primer-Rcgtgacacgaaccaattcac
protein_conc31462946
WB_result61.661.9
Ave_WB61.7
WB_DONE_date8/12/24
MW124kd
IP
antibody_Cat#A302-916Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID59125913




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NFX1Product_ID: A302-916A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NFX1-human		 NFX1-K562-CRISPR-A302-916A.png<br>Caption: Western blot following CRISPR against NFX1 in K562 whole cell lysate using NFX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NFX1. NFX1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-35BGKcLV44-36
Sample_IDBGKcLV44-35BGKcLV44-36
Sample Name
Sample NameNFX1-BGKcLV44-35NFX1-BGKcLV44-36
RBPNFX1NFX1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1359413595




Library-Prep Information
BGKcLV44-35BGKcLV44-36
Sample #2324
Sample NameNFX1-BGKcLV44-35NFX1-BGKcLV44-36
Sample_Name_Alias
Index Well PositionG08H08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp291286
Peak_Molarity67.8051.90
libSampleQC_DNA_WellDNA_Library_set75/set1/G8.pngDNA_Library_set75/set1/H8.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/G2.pngRNA_Library_set75/set1/H2.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-35BGKcLV44-36
RBPNFX1NFX1
Batch_IDBGKcLV44BGKcLV44
WB_result61.60061.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID45824583




Sequencing Information
BGKcLV44-35BGKcLV44-36
Sample_IDBGKcLV44-35BGKcLV44-36
Sample NameNFX1-BGKcLV44-35NFX1-BGKcLV44-36
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads50,530,09256,370,464
total_aligned_reads48,711,31253,929,379
unique_aligned_reads45,069,66849,925,734
percent_uniqueAligned0.891940.88567
correlation_replicates0.9979040.997904
spikein_reads70,214116,490
percent_spikeins0.001390.00207
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32933294




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database