DDX10

General Information

Gene Name       : DDX10
Gene ID             : ENSG00000178105.10
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: DDX10)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DDX10ENSG00000178105.1036.3335.0835.70ENCSR000AEF
HepG2DDX10ENSG00000178105.1023.8129.9626.88ENCSR985KAT
K562DDX10ENSG00000178105.1044.0463.6653.85ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq DDX10-BGKcLV28-K562ENCSR002ODCDDX10K562ReleasedENCSR220DTX   CRISPRKD% = 61.1,62.6






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DDX10ENCAB959QKKA300-617A1Released, ENCODE4BGKcLV28-67, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DDX10K562CRISPRENCSR002ODCENCSR220DTXDDX10-BGKcLV28-67,DDX10-BGKcLV28-68NT-BGKcLV28-1,NT-BGKcLV28-261.1,62.6ReleasedENCODE4
DDX10HepG2CRISPRBGHcLV29-67,BGHcLV29-68BGHcLV29-1,BGHcLV29-2no clear band,NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX10Product_ID: A300-617A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX10-human		 Bethyl_A300-617A_1_DDX10.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX10 specific antibody, A300-617A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX10-specific antibody, A300-617A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DDX10-K562-CRISPR-A300-617A.png<br>Caption: Western blot following CRISPR against DDX10 in K562 whole cell lysate using DDX10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX10. DDX10 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for DDX10 is not avaliable on ENCORE database.



Proteins bound to RNA
  • DDX10 RNA is bound by 20 proteins.  







eCLIP assay for DDX10 is not avaliable on ENCORE database.





20 proteins are bound to DDX10 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr11108810999108811217+3'-UTR,CDS3.151078.18115K562PUM1
chr11108811121108811203+3'-UTR,CDS3.393415.23038K562ZNF622
chr11108811188108811276+3'-UTR3.723917.52358K562UCHL5
chr11108811433108811474+3'-UTR4.333623.77023K562ZNF622
chr11108811156108811297+3'-UTR4.595054.69071HepG2GRWD1
chr11108811364108811454+3'-UTR3.275903.68829K562YBX3
chr11108712172108712206+CDS,intron-Proximal3.714554.74268K562UCHL5
chr11108594007108594145+CDS4.092393.75533K562TRA2A
chr11108594139108594210+CDS,intron-Proximal4.111823.31827HepG2GRWD1
chr11108550238108550288+CDS,intron-Proximal4.826266.13315HepG2BCLAF1
chr11108593955108594059+CDS3.751425.81804K562BUD13
chr11108559757108559790+CDS,intron-Proximal3.043193.19733K562ZNF622
chr11108712041108712105+CDS,intron-Proximal3.391107.58911K562UCHL5
chr11108722875108722937+CDS,intron-Proximal3.032527.13869K562ZNF800
chr11108722879108722937+CDS,intron-Proximal3.921058.82687K562UCHL5
chr11108594116108594189+CDS3.889528.61219K562UCHL5
chr11108562716108562766+CDS,intron-Proximal3.687844.51739HepG2BCLAF1
chr11108594097108594175+CDS3.504245.65306K562ZNF622
chr11108709271108709293+CDS,intron-Proximal3.220584.30396K562UCHL5
chr11108593911108594003+CDS4.340748.23108K562UCHL5
chr11108547887108547940+CDS3.807965.73766K562YBX3
chr11108810972108811039+CDS,intron-Proximal3.208984.56572K562ZNF800
chr11108810970108811015+CDS,intron-Proximal3.351884.62864K562ZNF622
chr11108811027108811121+CDS3.700046.14048K562ZNF622
chr11108594040108594158+CDS3.737683.89592K562XRCC6
chr11108788657108788736+CDS4.942346.17189K562PUM1
chr11108593722108593798+CDS,intron-Proximal3.053157.74050K562UCHL5
chr11108559662108559687+CDS,intron-Proximal3.291073.27569HepG2BCLAF1
chr11108811069108811137+CDS5.5850011.70549K562UCHL5
chr11108811041108811115+CDS4.533784.37348HepG2BCLAF1
chr11108535952108536058+CDS4.275887.82678K562ZNF622
chr11108593890108593930+CDS4.152333.87582K562ZNF622
chr11108562623108562688+CDS3.119344.26492HepG2BCLAF1
chr11108788598108788686+CDS,intron-Proximal4.0486810.54329K562UCHL5
chr11108593723108593817+CDS,intron-Proximal3.133944.74766HepG2BCLAF1
chr11108562602108562700+CDS,intron-Proximal3.890888.04012K562ZNF622
chr11108594006108594086+CDS5.6042311.70549K562UCHL5
chr11108709253108709271+CDS4.103353.71613K562UCHL5
chr11108594103108594196+CDS,intron-Proximal5.315898.41647HepG2BCLAF1
chr11108547811108547887+CDS,intron-Proximal3.059073.63043HepG2GRWD1
chr11108810971108811069+CDS,intron-Proximal6.0619616.27089K562UCHL5
chr11108590523108590554+CDS,intron-Proximal4.214554.16170K562UCHL5
chr11108810973108811118+CDS5.167259.05927K562GRWD1
chr11108810969108811041+CDS,intron-Proximal3.411824.26492HepG2BCLAF1
chr11108549074108549125+CDS4.530336.47760K562YBX3
chr11108712141108712203+CDS3.025206.13500K562ZNF800
chr11108549125108549160+CDS3.096653.69775K562YBX3
chr11108564242108564295+CDS,intron-Proximal3.570614.17155HepG2BCLAF1
chr11108547867108547887+CDS3.629405.11155K562YBX3
chr11108612279108612339+intron-Distal3.772284.91037K562EXOSC5
chr11108615916108616045+intron-Distal3.168834.13064K562ILF3
chr11108615943108616044+intron-Distal4.573039.08502K562PTBP1
chr11108657612108657679+intron-Distal4.093613.60235HepG2HNRNPM
chr11108615809108616051+intron-Distal3.824576.58297HepG2HNRNPK
chr11108615801108615919+intron-Distal6.5540914.93344HepG2PCBP2
chr11108611702108611731+intron-Distal4.064763.30696K562EXOSC5
chr11108755290108755404+intron-Distal4.732123.92866K562PTBP1
chr11108615817108615893+intron-Distal4.277703.23168HepG2DDX59
chr11108642521108642611+intron-Distal4.118213.59917K562EXOSC5
chr11108607027108607112+intron-Distal3.426443.50893K562TARDBP
chr11108659561108659629+intron-Distal4.270424.31871HepG2HNRNPM
chr11108642576108642624+intron-Distal3.834683.07980HepG2HNRNPM
chr11108615944108616006+intron-Distal5.1441616.92837HepG2PCBP2
chr11108740970108741061+intron-Distal4.352544.51735HepG2QKI
chr11108657812108657851+intron-Distal4.846057.06134K562EXOSC5
chr11108615953108616031+intron-Distal3.657884.81278HepG2PTBP1
chr11108656751108656776+intron-Distal3.118213.15041K562EXOSC5
chr11108754705108754811+intron-Distal4.201824.40847HepG2PTBP1
chr11108743526108743654+intron-Distal5.047467.89532HepG2PCBP2
chr11108656128108656175+intron-Distal4.428794.80399K562EXOSC5
chr11108656640108656690+intron-Distal4.664774.48623K562EXOSC5
chr11108780184108780261+intron-Distal4.709664.35687HepG2DDX59
chr11108626677108626773+intron-Distal4.635806.59655K562PTBP1
chr11108628412108628547+intron-Distal3.761576.86368K562PTBP1
chr11108628416108628550+intron-Distal4.412615.07733HepG2PTBP1
chr11108664508108664568+intron-Distal4.441194.30850HepG2QKI
chr11108741200108741269+intron-Distal4.441194.30850HepG2QKI
chr11108642624108642699+intron-Distal4.558415.16513HepG2HNRNPM
chr11108604567108604656+intron-Distal4.273354.28663HepG2SUGP2
chr11108656798108656826+intron-Distal4.618215.76606K562EXOSC5
chr11108642390108642499+intron-Distal4.482413.60235HepG2HNRNPM
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for DDX10 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for DDX10 is not available on ENCORE database.

  • 104 RBPs can affect the levels of DDX10.  








The Knockdown/knockout RNA-Seq assay for DDX10 is not available on ENCORE database.





104 RBPs can affect the gene expression of DDX10 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
DDX10ENSG00000178105.51066.66170565.936200.914309.291508.37970HepG2AKAP1
DDX10ENSG00000178105.5328.10960474.18020-0.531304.615203.64430HepG2AKAP8L
DDX10ENSG00000178105.5211.83930438.19160-1.048608.755807.27200HepG2BCLAF1
DDX10ENSG00000178105.5702.819701119.23540-0.671307.608106.67650HepG2BOP1
DDX10ENSG00000178105.5431.39860609.77030-0.499203.131701.82320HepG2BUD13
DDX10ENSG00000178105.5643.810501040.73160-0.692905.385604.00720HepG2CCAR1
DDX10ENSG00000178105.5504.614601027.54290-1.0259014.2072012.79290HepG2CPSF6
DDX10ENSG00000178105.5887.95790495.843700.840507.408206.21410HepG2DDX55
DDX10ENSG00000178105.5815.670601166.84260-0.516602.385201.47800HepG2DDX59
DDX10ENSG00000178105.5318.16930420.89070-0.403702.212301.33580HepG2DHX30
DDX10ENSG00000178105.5328.92450451.57070-0.457203.238602.51580HepG2DKC1
DDX10ENSG00000178105.5324.08770432.48720-0.416303.261902.56410HepG2DNAJC2
DDX10ENSG00000178105.5369.23500522.42270-0.500702.892901.95220HepG2EEF2
DDX10ENSG00000178105.5167.34420447.70580-1.4197014.5212013.23060HepG2EIF2S1
DDX10ENSG00000178105.5379.88010666.70950-0.811508.202706.83220HepG2EIF4G2
DDX10ENSG00000178105.5700.74990891.48900-0.347302.884102.27220HepG2ETF1
DDX10ENSG00000178105.5277.71700379.07000-0.448802.771401.60280HepG2FAM120A
DDX10ENSG00000178105.5340.46020463.07350-0.443802.215501.61720HepG2G3BP1
DDX10ENSG00000178105.5258.25180377.55640-0.547903.222902.24710HepG2GEMIN5
DDX10ENSG00000178105.5641.670601164.86520-0.860309.423408.28920HepG2GPKOW
DDX10ENSG00000178105.5534.83880369.737900.532502.596101.52120HepG2GRSF1
DDX10ENSG00000178105.5225.29970337.53040-0.583202.687201.34210HepG2HNRNPA0
DDX10ENSG00000178105.5227.64500440.57610-0.952608.105407.08370HepG2HNRNPA1
DDX10ENSG00000178105.5236.05390490.04120-1.0538010.414409.14400HepG2HNRNPC
DDX10ENSG00000178105.5532.27480787.02060-0.564206.815005.75770HepG2HNRNPF
DDX10ENSG00000178105.5531.859501111.64640-1.0636015.0517013.74930HepG2HNRNPLL
DDX10ENSG00000178105.5447.21730774.99570-0.7932010.187708.88290HepG2IGF2BP1
DDX10ENSG00000178105.5257.06270473.43490-0.881005.868304.35870HepG2KHSRP
DDX10ENSG00000178105.5794.959501098.61550-0.466702.989102.12390HepG2LARP4
DDX10ENSG00000178105.5827.20950502.127800.720105.851104.95940HepG2MATR3
DDX10ENSG00000178105.5706.095901133.59230-0.683003.112702.08030HepG2MTPAP
DDX10ENSG00000178105.5751.47260985.63980-0.391302.640701.90930HepG2NSUN2
DDX10ENSG00000178105.5605.73890495.831700.288701.957401.43280HepG2PCBP1
DDX10ENSG00000178105.5364.20690529.71060-0.540402.674901.65330HepG2PCBP2
DDX10ENSG00000178105.5477.38430667.77530-0.484203.784103.09920HepG2PUF60
DDX10ENSG00000178105.5348.94310480.76420-0.462303.865603.16100HepG2PUS1
DDX10ENSG00000178105.51383.18920745.116300.8924016.2331015.18890HepG2RBM22
DDX10ENSG00000178105.5665.00270522.924300.346702.618102.01650HepG2RECQL
DDX10ENSG00000178105.5649.54940847.73720-0.384202.589101.92510HepG2RPLP0
DDX10ENSG00000178105.5346.91760502.17590-0.533602.487401.59110HepG2RPS10
DDX10ENSG00000178105.5276.69840400.87410-0.534802.520901.75650HepG2RPS3A
DDX10ENSG00000178105.5235.68310414.11260-0.813205.969004.60390HepG2SERBP1
DDX10ENSG00000178105.5411.53910640.28350-0.637708.147707.04090HepG2SLBP
DDX10ENSG00000178105.5754.216801224.31660-0.698904.719103.68690HepG2SNRNP70
DDX10ENSG00000178105.51459.42790841.575300.7941019.6648018.64680HepG2SRFBP1
DDX10ENSG00000178105.51028.08620548.277400.9069018.0476017.06170HepG2SRSF1
DDX10ENSG00000178105.5545.67040773.20400-0.502805.520804.59400HepG2SRSF7
DDX10ENSG00000178105.5364.14990446.04300-0.292701.844901.37760HepG2SRSF9
DDX10ENSG00000178105.5398.45670730.67580-0.8748014.7403013.74580HepG2SSB
DDX10ENSG00000178105.5403.11820554.98610-0.461202.837501.88570HepG2SUPT6H
DDX10ENSG00000178105.5243.79870493.59600-1.017608.425707.35160HepG2SUPV3L1
DDX10ENSG00000178105.5320.178601083.33810-1.7585032.0339030.08470HepG2TARDBP
DDX10ENSG00000178105.5233.74880473.08850-1.0172011.6998010.79750HepG2TIAL1
DDX10ENSG00000178105.5353.57410477.24380-0.432703.548102.92700HepG2TRIM56
DDX10ENSG00000178105.5450.01760652.38110-0.535704.472003.33000HepG2TUFM
DDX10ENSG00000178105.51074.62880702.942600.612305.857704.95990HepG2U2AF1
DDX10ENSG00000178105.5414.00280591.68050-0.515205.236604.32460HepG2UPF1
DDX10ENSG00000178105.5302.67050451.95950-0.578402.655301.67120HepG2UPF2
DDX10ENSG00000178105.5713.98290502.706800.506102.495801.30770HepG2XRCC5
DDX10ENSG00000178105.5779.26060562.160400.471002.460001.46210HepG2XRCC6
DDX10ENSG00000178105.5290.40660485.89140-0.742605.257304.26650HepG2XRN2
DDX10ENSG00000178105.5413.10930650.73760-0.655609.293308.44230HepG2ZNF622
DDX10ENSG00000178105.51573.827501242.705300.340704.290303.61060K562AGGF1
DDX10ENSG00000178105.51656.471602063.52990-0.317002.386301.83510K562AQR
DDX10ENSG00000178105.51667.323501356.718200.297303.028502.07350K562ATP5C1
DDX10ENSG00000178105.5862.144201157.75070-0.425305.356204.00370K562BCLAF1
DDX10ENSG00000178105.51206.119002287.66370-0.923507.432005.97480K562BOP1
DDX10ENSG00000178105.5941.878501274.10100-0.435903.899302.36640K562CIRBP
DDX10ENSG00000178105.51953.962303581.48810-0.874207.083805.33100K562CNOT7
DDX10ENSG00000178105.51310.819002432.51680-0.8920014.9360013.08130K562CPSF6
DDX10ENSG00000178105.5642.71460885.86400-0.462904.173302.85540K562DDX3X
DDX10ENSG00000178105.53600.004802591.943200.4739011.000009.79850K562DDX47
DDX10ENSG00000178105.5700.56580898.15100-0.358403.129201.71520K562DDX6
DDX10ENSG00000178105.52107.184701391.539400.598508.519106.82650K562DKC1
DDX10ENSG00000178105.5638.23170973.05420-0.608409.304107.03150K562EIF2S1
DDX10ENSG00000178105.51081.746501884.09780-0.8005019.6760018.01660K562EIF2S2
DDX10ENSG00000178105.51227.76600945.427000.376904.191803.22070K562EIF3G
DDX10ENSG00000178105.52078.605002673.49460-0.363105.004003.75910K562EIF4G2
DDX10ENSG00000178105.51736.248502469.77990-0.508402.343501.56300K562FIP1L1
DDX10ENSG00000178105.51402.345702474.88510-0.8195011.044709.28010K562FMR1
DDX10ENSG00000178105.51307.966402097.62620-0.681405.263403.95880K562FTO
DDX10ENSG00000178105.51594.55510962.494700.7282015.3867014.06770K562FXR1
DDX10ENSG00000178105.51235.815402322.51410-0.9102015.1006013.21320K562GPKOW
DDX10ENSG00000178105.51122.792901405.43180-0.323902.878801.78400K562HNRNPA1
DDX10ENSG00000178105.5546.163801253.84200-1.1989011.5634010.41610K562HNRNPC
DDX10ENSG00000178105.51662.850101187.402500.485704.339802.75540K562HNRNPK
DDX10ENSG00000178105.5913.241101188.43560-0.380002.270401.36700K562IGF2BP2
DDX10ENSG00000178105.51384.738301982.98240-0.518102.569001.67320K562KRR1
DDX10ENSG00000178105.51558.049201249.360300.318502.791401.94770K562MAGOH
DDX10ENSG00000178105.52008.462601537.349600.385503.886202.77910K562MBNL1
DDX10ENSG00000178105.51532.674002267.00790-0.564705.458004.20310K562METAP2
DDX10ENSG00000178105.51660.320202849.74970-0.7794012.4632010.21730K562MSI2
DDX10ENSG00000178105.51601.622901077.565900.571704.230503.07110K562NAA15
DDX10ENSG00000178105.51568.546702061.39740-0.394208.925007.68720K562NSUN2
DDX10ENSG00000178105.51168.034801620.67880-0.472503.999502.99970K562PCBP3
DDX10ENSG00000178105.51029.488301243.09520-0.272002.094201.39720K562POLR2G
DDX10ENSG00000178105.51466.768201019.995300.524005.175204.15000K562PRPF8
DDX10ENSG00000178105.5862.929001124.13330-0.381502.251401.44360K562PSIP1
DDX10ENSG00000178105.51795.127501213.804800.564406.108105.15330K562PTBP1
DDX10ENSG00000178105.52370.496003040.07860-0.358904.888903.60120K562PUM2
DDX10ENSG00000178105.52100.440401282.487500.711609.731008.76070K562PUS1
DDX10ENSG00000178105.51616.997901249.498600.371904.221703.50550K562RBM15
DDX10ENSG00000178105.5729.70680962.69780-0.399803.338101.86180K562RBM25
DDX10ENSG00000178105.51574.286901107.692800.507005.898204.31860K562SAFB
DDX10ENSG00000178105.52219.544003262.07910-0.555503.325901.63580K562SBDS
DDX10ENSG00000178105.5549.81600872.88380-0.666804.195202.23200K562SERBP1
DDX10ENSG00000178105.51383.665702033.95570-0.5558010.869009.32720K562SF3A3
DDX10ENSG00000178105.51586.900901189.892800.415304.450003.55730K562SRSF1
DDX10ENSG00000178105.5780.50920977.63380-0.324902.284001.34490K562SRSF4
DDX10ENSG00000178105.5839.935201123.66380-0.419903.466002.26800K562SRSF5
DDX10ENSG00000178105.51139.160801411.66120-0.309403.631502.27930K562SRSF7
DDX10ENSG00000178105.5689.21880987.69510-0.519103.766102.56440K562SUPV3L1
DDX10ENSG00000178105.52086.259201246.885500.7425014.7249012.83480K562SYNCRIP
DDX10ENSG00000178105.5785.268301204.33650-0.617005.990804.92130K562TARDBP
DDX10ENSG00000178105.5866.722601161.69560-0.422604.112003.08530K562TIA1
DDX10ENSG00000178105.5608.397101081.26760-0.829607.208105.64010K562TIAL1
DDX10ENSG00000178105.5910.800901139.76210-0.323502.351301.36160K562TRA2A
DDX10ENSG00000178105.51601.107902117.37380-0.403204.848503.15850K562TROVE2
DDX10ENSG00000178105.5614.702001045.29400-0.765908.371206.52670K562UCHL5
DDX10ENSG00000178105.51315.393702185.73710-0.7326021.8625020.08210K562UPF1
DDX10ENSG00000178105.51272.868701870.27640-0.555207.762306.25350K562WDR3
DDX10ENSG00000178105.51542.73350943.133100.709903.785401.86000K562XRCC6
DDX10ENSG00000178105.51019.829002324.72300-1.1887029.0109026.14880K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for DDX10 is not available on ENCORE database.





1 RBPs can affect the splicing of DDX10 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A3SSDDX10ENSG00000178105.5chr11+1085774441085775641085774651085775641085642421085643270.066000.007080.03919longerK562AQR
A3SSDDX10ENSG00000178105.5chr11+1087885761087887451087885991087887451087228791087229360.064000.001150.00788longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for DDX10 is not avaliable on ENCORE database.