DDX10      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-25BGHcLV49-26
idx00
TRCN#_or_BGC#BGC#0000743BGC#0000743
shRNA_or_gRNA_sequenceAATATACTCGTGTGCACACCAATATACTCGTGTGCACACC
PAMAGGAGG
NameDDX10_75DDX10_75
Sample_IDBGHcLV49-25BGHcLV49-26
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameDDX10DDX10
qPCR_result46.057.8
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc52195658
WB_result91.982.6721
Ave_WB
WB_DONE_date7/10/25
MW101KD
IPRabbit / I
antibody_Cat#A300-617ALOT #1
Antibody DCC IDENCAB959QKK
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99059906




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX10Product_ID: A300-617A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX10-human		 DDX10-hepg2-CRISPR-A300-617A-LICOR.png<br>Caption: Western blot following CRISPR against DDX10 in HepG2 whole cell lysate using DDX10 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX10. DDX10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A300-617A_1_DDX10.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX10 specific antibody, A300-617A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX10-specific antibody, A300-617A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DDX10-K562-CRISPR-A300-617A.png<br>Caption: Western blot following CRISPR against DDX10 in K562 whole cell lysate using DDX10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX10. DDX10 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-25BGHcLV49-26
Sample_IDBGHcLV49-25BGHcLV49-26
Sample Name
Sample Name
RBPDDX10DDX10
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1434014341




Library-Prep Information
BGHcLV49-25BGHcLV49-26
Sample #3738
Sample NameDDX10-BGHcLV49-25DDX10-BGHcLV49-26
Sample_Name_Alias
Index Well PositionG05H05
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp258260
Peak_Molarity72.8058.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-25BGHcLV49-26
RBPDDX10DDX10
Batch_IDBGHcLV49BGHcLV49
WB_result91.90082.672
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID47824783




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database