BRIX1

General Information

Gene Name       : BRIX1
Gene ID             : ENSG00000113460.12
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: BRIX1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878BRIX1ENSG00000113460.1271.9956.5364.26ENCSR000AEF
HepG2BRIX1ENSG00000113460.1227.0568.3947.72ENCSR985KAT
K562BRIX1ENSG00000113460.12102.28129.90116.09ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq BRIX1-BGKcLV23-K562ENCSR162HOUBRIX1K562ReleasedENCSR877JEM   CRISPRKD% = 70.1,70.4
knockdown followed by RNA-seq BRIX1-BGHcLV24-HepG2ENCSR060SLEBRIX1HepG2ReleasedENCSR671FDZ   CRISPRKD% = 84.3,83.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
BRIX1ENCAB292SQJPA5-89858UK2881309ABGHcLV24-19, Released, ENCODE4BGKcLV23-23, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
BRIX1K562CRISPRENCSR162HOUENCSR877JEMBRIX1-BGKcLV23-23,BRIX1-BGKcLV23-24NT-BGKcLV23-1,NT-BGKcLV23-270.1,70.4ReleasedENCODE4
BRIX1K562CRISPRBGKcLV23-25,BGKcLV23-26BGKcLV23-1,BGKcLV23-278.2,65.4NotSatisfiedENCODE4
BRIX1HepG2CRISPRENCSR060SLEENCSR671FDZBRIX1-BGHcLV24-19,BRIX1-BGHcLV24-20NT-BGHcLV24-1,NT-BGHcLV24-284.3,83.5ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
BRIX1Product_ID: PA5-89858
Lot_ID: UK2881309A
Source: Thermo Fisher
Target Name: BRIX1-human		 brix1-HEPG2-CRISPR-PA5-89858.png<br>Caption: Western blot following CRISPR against BRIX1 in HepG2 whole cell lysate using BRIX1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BRIX1. BRIX1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 brix1-K562-CRISPR-PA5-89858.png<br>Caption: Western blot following CRISPR against BRIX1 in K562 whole cell lysate using BRIX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BRIX1. BRIX1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for BRIX1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • BRIX1 RNA is bound by 14 proteins.  







eCLIP assay for BRIX1 is not avaliable on ENCORE database.





14 proteins are bound to BRIX1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr53492550434925609+3'-UTR,CDS3.528928.66760K562UCHL5
chr53492566734925727+3'-UTR3.373755.27768K562DDX6
chr53492568034925758+3'-UTR4.6697011.99854K562TIA1
chr53492567234925749+3'-UTR4.3138144.46745K562PUM2
chr53492565634925736+3'-UTR3.410529.30162K562ZC3H11A
chr53491582834916003+5'-UTR,CDS,intron-Proximal4.2432233.30951K562UCHL5
chr53491583134915957+5'-UTR,CDS3.354926.39282HepG2GRWD1
chr53491600134916012+CDS,intron-Proximal3.477548.23725K562AQR
chr53492237834922424+CDS,intron-Proximal4.660705.33214K562PRPF8
chr53491994434919962+CDS,intron-Distal,intron-Proximal3.202103.07653K562ZNF622
chr53492543834925559+CDS3.611844.46864HepG2BCLAF1
chr53492239134922445+CDS,intron-Proximal3.405347.17950K562AQR
chr53492507634925081+CDS,intron-Proximal4.3618110.75300K562YBX3
chr53492532934925347+CDS,intron-Proximal4.5242912.06297K562YBX3
chr53492237334922538+CDS,intron-Distal,intron-Proximal3.6273412.75661K562KHSRP
chr53491995734919984+CDS,intron-Distal3.494803.12941K562UCHL5
chr53491591234915979+CDS3.243704.63095K562DDX6
chr53492331734923362+CDS,intron-Proximal4.137805.55177K562PRPF8
chr53491846934918519+CDS,intron-Distal3.175635.10726K562UCHL5
chr53492552334925594+CDS3.494913.93112K562RBM15
chr53492504434925076+CDS3.9827812.08941K562YBX3
chr53491590334916004+CDS,intron-Proximal4.1410928.03000K562ZNF622
chr53492324134923339+CDS3.987113.74511K562IGF2BP1
chr53491599934916049+CDS,intron-Proximal3.598744.54206HepG2PRPF8
chr53491996234919989+CDS,intron-Distal,intron-Proximal3.305323.61259K562ZNF622
chr53492507934925085+CDS,intron-Proximal3.4701912.03964K562AQR
chr53491866434918718+intron-Distal,intron-Proximal4.245663.49003K562PRPF8
chr53492510534925138+intron-Proximal5.3325512.70303K562AQR
chr53492287934922942+intron-Proximal4.172065.97994K562AQR
chr53491601234916048+intron-Proximal3.877538.13013K562AQR
chr53492508534925105+intron-Proximal5.2642713.23057K562AQR
chr53492256334922614+intron-Proximal4.287885.25174K562KHSRP
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for BRIX1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for BRIX1 is not available on ENCORE database.

  • 105 RBPs can affect the levels of BRIX1.  








The Knockdown/knockout RNA-Seq assay for BRIX1 is not available on ENCORE database.





105 RBPs can affect the gene expression of BRIX1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
BRIX1ENSG00000113460.8420.02110626.52150-0.576906.833505.96440HepG2ACO1
BRIX1ENSG00000113460.81069.98280844.328800.341601.825201.38310HepG2AKAP1
BRIX1ENSG00000113460.8499.38870629.49840-0.334002.607301.85950HepG2AKAP8L
BRIX1ENSG00000113460.8746.245501044.31350-0.484806.525905.54090HepG2AUH
BRIX1ENSG00000113460.8366.61510665.96690-0.861207.307705.92840HepG2BCLAF1
BRIX1ENSG00000113460.8491.15680663.74170-0.434402.582301.83300HepG2CIRBP
BRIX1ENSG00000113460.8522.71650626.42540-0.261101.937701.36790HepG2CKAP4
BRIX1ENSG00000113460.8576.09630884.43840-0.618505.573704.62910HepG2CPSF6
BRIX1ENSG00000113460.8408.40570576.35940-0.497002.875902.18590HepG2DDX21
BRIX1ENSG00000113460.8471.81040753.48010-0.675404.568603.60150HepG2DDX55
BRIX1ENSG00000113460.8642.591901004.34340-0.644303.301802.23640HepG2DDX59
BRIX1ENSG00000113460.8418.15720694.71240-0.732405.644704.78990HepG2DDX6
BRIX1ENSG00000113460.8472.48170599.42170-0.343302.506501.85700HepG2DKC1
BRIX1ENSG00000113460.8465.58610595.75360-0.355702.115101.49150HepG2DNAJC21
BRIX1ENSG00000113460.8389.48050590.46090-0.600307.391606.39650HepG2DNAJC2
BRIX1ENSG00000113460.8300.28460667.94740-1.1534013.1047011.87960HepG2EIF2S1
BRIX1ENSG00000113460.8552.62200738.21040-0.417702.288201.70450HepG2EIF4A3
BRIX1ENSG00000113460.8686.36140966.59740-0.493904.272903.27490HepG2EIF4G2
BRIX1ENSG00000113460.8493.70440690.87200-0.484803.021402.32740HepG2G3BP1
BRIX1ENSG00000113460.8412.00530602.98440-0.549503.473602.32420HepG2GNB2L1
BRIX1ENSG00000113460.8562.238401002.59120-0.834508.514307.43220HepG2GPKOW
BRIX1ENSG00000113460.8361.93270601.46070-0.732706.676205.73840HepG2HNRNPA1
BRIX1ENSG00000113460.8773.76850939.97930-0.280702.498801.60460HepG2HNRNPA2B1
BRIX1ENSG00000113460.8484.25090669.00320-0.466303.279402.53040HepG2HNRNPC
BRIX1ENSG00000113460.8648.65270986.48380-0.604808.849807.65960HepG2HNRNPF
BRIX1ENSG00000113460.8483.06710956.79020-0.9860012.3596011.15300HepG2HNRNPLL
BRIX1ENSG00000113460.8557.33220971.41180-0.8015011.6067010.22140HepG2IGF2BP1
BRIX1ENSG00000113460.8541.74140702.28470-0.374502.148501.54460HepG2IGF2BP2
BRIX1ENSG00000113460.8512.21640678.84930-0.406304.772503.83180HepG2ILF2
BRIX1ENSG00000113460.8898.643201256.49880-0.483602.034301.33130HepG2LARP4
BRIX1ENSG00000113460.8969.31390749.125100.371702.115701.55940HepG2MATR3
BRIX1ENSG00000113460.8680.45570975.77680-0.520002.846001.86350HepG2MTPAP
BRIX1ENSG00000113460.8568.08700848.36930-0.578604.845003.86560HepG2NSUN2
BRIX1ENSG00000113460.8501.29900682.96960-0.446102.730902.01360HepG2PABPC4
BRIX1ENSG00000113460.8401.87140581.38450-0.532803.041402.19780HepG2PRPF8
BRIX1ENSG00000113460.8572.57240718.59980-0.327702.698002.15630HepG2PTBP1
BRIX1ENSG00000113460.8645.39240968.15450-0.585104.778304.02080HepG2PUF60
BRIX1ENSG00000113460.8613.78030778.26220-0.342502.599801.99130HepG2QKI
BRIX1ENSG00000113460.81007.88250642.671000.649103.441402.65690HepG2RAVER1
BRIX1ENSG00000113460.8576.23900799.73290-0.472904.895904.21190HepG2NELFE
BRIX1ENSG00000113460.8634.497801062.58270-0.743908.855207.74100HepG2RPLP0
BRIX1ENSG00000113460.8419.35500533.40220-0.347101.942101.41940HepG2RPS19
BRIX1ENSG00000113460.8369.31980535.38730-0.535703.383102.34050HepG2SERBP1
BRIX1ENSG00000113460.81317.35650830.308700.665805.495904.52620HepG2SF3A3
BRIX1ENSG00000113460.8660.88660928.27320-0.490106.982005.94880HepG2SLBP
BRIX1ENSG00000113460.81092.01570780.912500.483706.475305.82540HepG2SRSF1
BRIX1ENSG00000113460.8594.42340969.16420-0.7053011.6034010.34600HepG2SRSF7
BRIX1ENSG00000113460.8527.48420635.31370-0.268301.980301.49910HepG2SRSF9
BRIX1ENSG00000113460.8614.54300982.76670-0.6773011.5640010.65720HepG2SSB
BRIX1ENSG00000113460.8438.27580586.57100-0.420502.793201.84910HepG2SUPT6H
BRIX1ENSG00000113460.8397.68060750.11710-0.915507.867606.82100HepG2SUPV3L1
BRIX1ENSG00000113460.8574.20540701.91380-0.289702.092801.59250HepG2TAF15
BRIX1ENSG00000113460.8537.99370932.54410-0.793609.097707.89210HepG2TARDBP
BRIX1ENSG00000113460.8414.69200645.80440-0.639106.669205.95410HepG2TIAL1
BRIX1ENSG00000113460.8467.85560651.53470-0.477804.334703.66010HepG2TRIM56
BRIX1ENSG00000113460.8474.67970736.35490-0.633408.834407.70530HepG2UPF1
BRIX1ENSG00000113460.8550.51780756.88450-0.459304.632503.31530HepG2UTP18
BRIX1ENSG00000113460.81238.71560710.924700.8010017.3108016.00300HepG2UTP3
BRIX1ENSG00000113460.8628.82050809.85220-0.365004.081203.45180HepG2ZNF622
BRIX1ENSG00000113460.83131.903402361.462400.407303.336102.02440K562AARS
BRIX1ENSG00000113460.83515.895102368.518800.5698015.8992014.65420K562AGGF1
BRIX1ENSG00000113460.83635.867702464.549500.560908.206407.02100K562AKAP8L
BRIX1ENSG00000113460.83342.180302585.902500.370005.717404.34870K562ATP5C1
BRIX1ENSG00000113460.81848.029402397.60390-0.375605.346103.99560K562BCLAF1
BRIX1ENSG00000113460.82080.006703303.61830-0.667505.262104.04830K562BOP1
BRIX1ENSG00000113460.83449.232605432.44450-0.655304.274302.90870K562CNOT7
BRIX1ENSG00000113460.81919.682503512.84290-0.871809.019707.58490K562CPSF6
BRIX1ENSG00000113460.81411.277701818.94040-0.366104.437103.05910K562CSTF2
BRIX1ENSG00000113460.82471.469902101.933500.233602.583201.30820K562DDX27
BRIX1ENSG00000113460.81315.400001738.57760-0.402404.256602.92450K562DDX3X
BRIX1ENSG00000113460.86051.832104656.021100.378208.454607.39640K562DDX47
BRIX1ENSG00000113460.83985.156402652.042400.5874014.5203012.18340K562DKC1
BRIX1ENSG00000113460.81428.223101909.70110-0.419106.858604.92220K562EIF2S1
BRIX1ENSG00000113460.82495.115703834.71620-0.6200013.2305011.83980K562EIF2S2
BRIX1ENSG00000113460.82625.181501969.593400.414406.159004.98900K562EIF3G
BRIX1ENSG00000113460.82248.368803573.94630-0.668606.551805.21440K562FMR1
BRIX1ENSG00000113460.81922.441403029.11690-0.656004.822303.58200K562FTO
BRIX1ENSG00000113460.82848.740701888.974100.5926013.7461012.50090K562FXR1
BRIX1ENSG00000113460.82823.498102107.286600.422002.074801.47430K562G3BP2
BRIX1ENSG00000113460.82118.803803354.01940-0.662605.951304.78880K562GPKOW
BRIX1ENSG00000113460.84115.305805110.47870-0.312503.315202.49100K562GRSF1
BRIX1ENSG00000113460.82174.536502616.73770-0.267102.485401.46700K562HNRNPA1
BRIX1ENSG00000113460.81224.795802585.10210-1.077709.838208.76850K562HNRNPC
BRIX1ENSG00000113460.81620.067702307.97610-0.510603.436002.15430K562HNRNPF
BRIX1ENSG00000113460.82945.202402270.573800.375203.144101.82240K562HNRNPK
BRIX1ENSG00000113460.84463.430103084.160500.533205.442004.07970K562HNRNPM
BRIX1ENSG00000113460.83647.405502615.508900.479702.789901.96070K562KIF1C
BRIX1ENSG00000113460.81654.959102863.60240-0.791008.390606.66310K562KRR1
BRIX1ENSG00000113460.82982.050802389.113800.319703.218502.30600K562MAGOH
BRIX1ENSG00000113460.83592.415402930.029800.293903.182302.19480K562MBNL1
BRIX1ENSG00000113460.82168.942803273.84700-0.594005.591704.32050K562METAP2
BRIX1ENSG00000113460.82543.469503318.36880-0.383708.133406.81610K562MTPAP
BRIX1ENSG00000113460.83788.713302053.738200.883402.525201.69110K562NAA15
BRIX1ENSG00000113460.82634.590103687.13390-0.4849018.3980016.70680K562NSUN2
BRIX1ENSG00000113460.82761.754902125.806100.377503.814502.66580K562PCBP2
BRIX1ENSG00000113460.81874.738403099.62430-0.725409.550108.01460K562PCBP3
BRIX1ENSG00000113460.81624.142002377.52460-0.549808.436507.06530K562POLR2G
BRIX1ENSG00000113460.83190.746601926.663100.7277011.484109.94850K562PRPF8
BRIX1ENSG00000113460.81727.914702317.66920-0.423602.301801.48280K562PSIP1
BRIX1ENSG00000113460.83075.938602321.138300.406102.736102.07510K562PTBP1
BRIX1ENSG00000113460.87905.296406370.663000.311303.104101.97250K562PUM1
BRIX1ENSG00000113460.85978.469402444.169101.2903035.3062033.31740K562PUS1
BRIX1ENSG00000113460.84058.181002381.464400.7689021.7390020.01300K562RBM15
BRIX1ENSG00000113460.81518.987102113.87140-0.476803.323102.23520K562RCC2
BRIX1ENSG00000113460.83497.392904947.97390-0.500602.875101.32610K562SBDS
BRIX1ENSG00000113460.83021.705403638.06950-0.267803.679102.74670K562SF3A3
BRIX1ENSG00000113460.82413.609703189.35810-0.402102.861201.86980K562SF3B1
BRIX1ENSG00000113460.83038.648402191.863100.471207.349005.91230K562SF3B4
BRIX1ENSG00000113460.81406.350602003.96620-0.510903.230502.10170K562SRFBP1
BRIX1ENSG00000113460.83219.600703727.26360-0.211203.439902.41070K562SRPK2
BRIX1ENSG00000113460.83126.872202275.436200.458506.466605.39750K562SRSF1
BRIX1ENSG00000113460.81325.737701789.18850-0.432502.472701.48460K562SRSF4
BRIX1ENSG00000113460.81656.306902148.84660-0.375603.474702.27490K562SRSF5
BRIX1ENSG00000113460.83162.297702384.709400.407105.667604.48490K562SYNCRIP
BRIX1ENSG00000113460.81720.718402221.47780-0.368504.120303.09210K562TIA1
BRIX1ENSG00000113460.81405.540402229.29520-0.665503.311102.28190K562TIAL1
BRIX1ENSG00000113460.81718.411602367.55510-0.462303.117602.10060K562TRIM56
BRIX1ENSG00000113460.82865.070302043.383000.487504.371402.93250K562U2AF1
BRIX1ENSG00000113460.83033.160802110.128600.523408.819407.37600K562U2AF2
BRIX1ENSG00000113460.81407.283801974.53960-0.488604.563303.22320K562UCHL5
BRIX1ENSG00000113460.82146.435603909.53610-0.8651037.5500035.26280K562UPF1
BRIX1ENSG00000113460.82157.070503345.28310-0.6331011.429009.59560K562WDR3
BRIX1ENSG00000113460.83473.415804840.75330-0.478905.866904.53740K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for BRIX1 is not available on ENCORE database.





2 RBPs can affect the splicing of BRIX1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEBRIX1ENSG00000113460.8chr5+349226433492269334922321349223923492279934923001-0.081000.000000.00000excludedK562AQR
SEBRIX1ENSG00000113460.8chr5+349231053492315634922799349228733492323734923339-0.103000.000000.00000excludedK562AQR
SEBRIX1ENSG00000113460.8chr5+349248893492508034923237349233393492533034926101-0.060000.000000.00000excludedK562AQR
SEBRIX1ENSG00000113460.8chr5+349199443491998834915480349160023492232134922392-0.189000.000000.00000excludedK562AQR
A3SSBRIX1ENSG00000113460.8chr5+3492262434922693349226433492269334922272349223920.103000.000000.00000longerK562AQR
RIBRIX1ENSG00000113460.8chr5+3492262434922873349226243492269334922799349228730.085000.000000.00000includedK562PUS1
MXEBRIX1ENSG00000113460.8chr5+34922321349223923492488934925080349158563491600234925330349254500.072000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34922624349228733492488934925080349222723492239234925330349254500.075000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34922799349228733492495134925080349223213492239234925330349254500.074000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34922799349228733492310534923156349223213492239234923237349233390.304000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34922799349228733492323734923339349223213492239234924951349250800.085000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34922799349228733492488934925080349222723492239234925330349254500.074000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34923237349233393492495134925080349223213492239234925330349254500.053000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34923237349233393492488934925080349222723492239234925330349254500.054000.000000.000001includedK562AQR
MXEBRIX1ENSG00000113460.8chr5+34923237349233393492495134925080349227993492287334925330349254500.105000.000000.000001includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for BRIX1 is not avaliable on ENCORE database.