ACO2

General Information

Gene Name       : ACO2
Gene ID             : ENSG00000100412.15
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: ACO2)


Antibody Validation

 View validation images for all ACO2 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ACO2ENSG00000100412.1526.2669.5647.91ENCSR000AEF
HepG2ACO2ENSG00000100412.1569.3935.5852.48ENCSR985KAT
K562ACO2ENSG00000100412.1578.4774.7376.60ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ACO2-BGKcLV26-K562ENCSR274ESZACO2K562ReleasedENCSR900GQL   CRISPRKD% = 76.7,73.7
knockdown followed by RNA-seq ACO2-BGHcLV25-HepG2ENCSR251SXSACO2HepG2ReleasedENCSR953VMB   CRISPRKD% = 59.3,59.8






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ACO2ENCAB412MAWA305-302A1BGHcLV25-3, Released, ENCODE4BGKcLV26-3, Released, ENCODE4
ACO2ENCAB850ZAMA305-308A1Released, ENCODE4BGHcLV25-3, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ACO2K562CRISPRENCSR274ESZENCSR900GQLACO2-BGKcLV26-3,ACO2-BGKcLV26-4NT-BGKcLV26-1,NT-BGKcLV26-276.7,73.7ReleasedENCODE4
ACO2HepG2CRISPRENCSR251SXSENCSR953VMBACO2-BGHcLV25-3,ACO2-BGHcLV25-4NT-BGHcLV25-1,NT-BGHcLV25-259.3,59.8ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ACO2Product_ID: A305-308A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ACO2-human		 Bethyl_A305-308A_1_ACO2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ACO2 specific antibody, A305-308A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ACO2-specific antibody, A305-308A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
ACO2Product_ID: A305-302A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ACO2-human		 ACO2-HEPG2-CRISPR-A305-302A.png<br>Caption: Western blot following CRISPR against ACO2 in HepG2 whole cell lysate using ACO2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ACO2. ACO2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ACO2-K562-CRISPR-A305-302A.png<br>Caption: Western blot following CRISPR against ACO2 in K562 whole cell lysate using ACO2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ACO2. ACO2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ACO2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • ACO2 RNA is bound by 26 proteins.  







eCLIP assay for ACO2 is not avaliable on ENCORE database.





26 proteins are bound to ACO2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr224192480041924865+3'-UTR3.9823014.15020K562TIA1
chr224192487841924948+3'-UTR3.877168.02406K562PABPC4
chr224192480941924927+3'-UTR7.0274318.80239HepG2SUB1
chr224192461541924682+3'-UTR5.125335.44904HepG2RPS3
chr224191975941919855+CDS,intron-Proximal5.195694.90235K562SF3B4
chr224192228241922347+CDS3.822183.45072HepG2YBX3
chr224190397941904053+CDS3.262924.94718HepG2PPIG
chr224191626341916280+CDS3.117363.12941K562UCHL5
chr224192085341920893+CDS3.426597.44661K562YBX3
chr224192448241924489+CDS,intron-Proximal3.575635.73766K562YBX3
chr224192448241924531+CDS,intron-Proximal4.331135.24403K562IGF2BP2
chr224192448941924539+CDS3.258598.88089K562YBX3
chr224186516341865247+CDS,intron-Proximal4.6750712.48497HepG2PRPF8
chr224191362841913707+CDS,intron-Proximal3.233357.62597HepG2PRPF8
chr224189582641895867+CDS,intron-Distal,intron-Proximal3.250887.59396K562YBX3
chr224192132941921399+CDS,intron-Proximal3.469283.71591HepG2PRPF8
chr224190386241903936+CDS3.969435.94556K562FMR1
chr224192397041924027+CDS,intron-Proximal3.582974.97175K562IGF2BP2
chr224192124441921295+CDS3.436847.28841K562ZNF622
chr224191363441913642+CDS,intron-Proximal3.706366.21987K562AQR
chr224192087641920961+CDS4.525478.23215K562FXR2
chr224190387241903926+CDS5.1777212.33415K562RPS3
chr224192394641924030+CDS,intron-Proximal3.627664.14939K562PUM1
chr224192399741924024+CDS4.369843.49905HepG2PPIG
chr224191143141911540+CDS,intron-Proximal4.160183.66349K562LIN28B
chr224191883341918871+CDS,intron-Proximal3.613885.03335K562UCHL5
chr224192236141922399+CDS4.161094.07072K562UCHL5
chr224192398941924011+CDS3.646858.50021K562YBX3
chr224192401141924026+CDS3.729798.84704K562YBX3
chr224192390441924025+CDS,intron-Proximal4.334024.08466HepG2IGF2BP1
chr224192396441923989+CDS4.223179.44646K562YBX3
chr224192119641921283+CDS,intron-Proximal3.627033.80834K562METAP2
chr224187764341877712+intron-Distal3.030193.14895K562NONO
chr224188193941882005+intron-Distal3.055083.11910K562RBFOX2
chr224191156241911648+intron-Distal,intron-Proximal4.515865.59467HepG2PRPF4
chr224189736641897432+intron-Distal4.860243.22788K562XRCC6
chr224191156441911645+intron-Distal,intron-Proximal4.895095.54520K562GTF2F1
chr224192097241921015+intron-Proximal4.784594.65563K562AQR
chr224191363841913671+intron-Proximal4.114913.44423K562BUD13
chr224192221441922258+intron-Proximal5.626975.12752HepG2SF3A3
chr224191364841913678+intron-Proximal3.501677.17950K562AQR
chr224192135541921405+intron-Proximal5.118604.48002K562AQR
chr224191460341914663+intron-Proximal5.024574.83371K562EFTUD2
chr224191994541919994+intron-Proximal5.577079.95272K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for ACO2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ACO2 is not available on ENCORE database.

  • 103 RBPs can affect the levels of ACO2.  








The Knockdown/knockout RNA-Seq assay for ACO2 is not available on ENCORE database.





103 RBPs can affect the gene expression of ACO2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
ACO2ENSG00000100412.111006.671901354.08730-0.427703.648302.42690HepG2AATF
ACO2ENSG00000100412.111781.968902154.19560-0.273703.973603.26950HepG2ACO1
ACO2ENSG00000100412.111828.375402424.88690-0.407402.775301.73580HepG2AKAP8
ACO2ENSG00000100412.111466.563102164.16040-0.5614012.4056010.93860HepG2AKAP8L
ACO2ENSG00000100412.113200.686902739.005800.224603.020502.30830HepG2AUH
ACO2ENSG00000100412.111896.937601306.255000.538104.740003.59120HepG2BCLAF1
ACO2ENSG00000100412.112000.372301481.430900.433204.298303.57110HepG2BOP1
ACO2ENSG00000100412.111862.310101359.984800.453404.195303.36360HepG2CPSF6
ACO2ENSG00000100412.112401.976902000.572300.263702.251701.66180HepG2CELF1
ACO2ENSG00000100412.112612.924602150.767200.280704.675003.74950HepG2DAZAP1
ACO2ENSG00000100412.111176.680701461.76630-0.313002.594701.71220HepG2DDX24
ACO2ENSG00000100412.114013.609303304.873700.280201.886101.35950HepG2DDX3X
ACO2ENSG00000100412.11647.384401214.41470-0.9076014.2253012.25830HepG2DHX30
ACO2ENSG00000100412.111058.391001353.63530-0.355002.461301.60390HepG2EIF3D
ACO2ENSG00000100412.111129.720401410.29630-0.320002.729101.65470HepG2EIF3G
ACO2ENSG00000100412.112109.935702936.84710-0.477103.214902.47630HepG2EIF4B
ACO2ENSG00000100412.111792.228802122.15610-0.243802.513401.70730HepG2EIF4G1
ACO2ENSG00000100412.112746.351603144.65260-0.195402.215701.66760HepG2ETF1
ACO2ENSG00000100412.111948.634202547.63530-0.386702.347701.63460HepG2FIP1L1
ACO2ENSG00000100412.112016.361501541.513200.387302.747602.10820HepG2GPKOW
ACO2ENSG00000100412.112304.161701976.780300.221002.715101.78730HepG2HNRNPA2B1
ACO2ENSG00000100412.112434.485901999.871700.283603.708802.88800HepG2HNRNPF
ACO2ENSG00000100412.112064.081802859.83330-0.470405.210104.27510HepG2HNRNPK
ACO2ENSG00000100412.112239.673401959.081400.193002.877402.19250HepG2HNRNPL
ACO2ENSG00000100412.112038.897101471.106700.470804.377403.60080HepG2HNRNPLL
ACO2ENSG00000100412.111237.972801757.32980-0.505406.793605.40190HepG2HNRNPM
ACO2ENSG00000100412.112620.659601969.295900.412205.281604.31430HepG2IGF2BP1
ACO2ENSG00000100412.111659.309201434.753800.209702.558401.85110HepG2ILF2
ACO2ENSG00000100412.112297.362401969.088000.222403.203302.10800HepG2KIF1C
ACO2ENSG00000100412.111847.686602637.84790-0.513603.678302.23100HepG2KRR1
ACO2ENSG00000100412.111754.520102739.16700-0.642705.489504.30560HepG2LARP4
ACO2ENSG00000100412.112195.328903044.82160-0.471904.420203.59320HepG2MAGOH
ACO2ENSG00000100412.111659.161702061.20770-0.313003.165702.30310HepG2MSI2
ACO2ENSG00000100412.112256.597402790.07140-0.306202.128801.48830HepG2PABPC4
ACO2ENSG00000100412.112109.239502969.66190-0.4936011.3126010.21980HepG2PCBP1
ACO2ENSG00000100412.11925.080001252.79250-0.437505.517304.50710HepG2PPIL4
ACO2ENSG00000100412.111221.210901813.67490-0.570608.158706.92320HepG2PRPF4
ACO2ENSG00000100412.112500.195803021.82950-0.273403.914003.28830HepG2PTBP1
ACO2ENSG00000100412.112510.071302161.367800.215703.756503.08840HepG2PUM1
ACO2ENSG00000100412.112764.917602194.160400.333506.202205.34560HepG2PUS1
ACO2ENSG00000100412.112820.756503272.52960-0.214302.041101.49300HepG2QKI
ACO2ENSG00000100412.111736.773302628.36700-0.5978012.0504011.12600HepG2RBM22
ACO2ENSG00000100412.112455.346002970.10130-0.274602.503101.85830HepG2RBM25
ACO2ENSG00000100412.111881.137803362.93340-0.8381029.8486028.46960HepG2NELFE
ACO2ENSG00000100412.111896.751203132.24730-0.7237022.9872021.51370HepG2RECQL
ACO2ENSG00000100412.111178.220501544.84270-0.390903.493302.28120HepG2RPS5
ACO2ENSG00000100412.11876.655301127.79850-0.363402.214101.50360HepG2RRP9
ACO2ENSG00000100412.111112.407401685.62450-0.599603.007901.41620HepG2RTF1
ACO2ENSG00000100412.112392.124001250.865200.9353038.0993036.13750HepG2SAFB2
ACO2ENSG00000100412.111647.835601321.921700.317802.223501.37720HepG2SERBP1
ACO2ENSG00000100412.112131.371901456.026800.549606.440305.40180HepG2SF3B1
ACO2ENSG00000100412.112776.994301952.390900.5082015.4566014.04360HepG2SLBP
ACO2ENSG00000100412.112105.338801581.522900.412602.522201.61030HepG2SMNDC1
ACO2ENSG00000100412.112192.212101509.391000.538303.474501.67200HepG2SND1
ACO2ENSG00000100412.111205.948501620.11320-0.425902.217401.52190HepG2SNRNP70
ACO2ENSG00000100412.111170.762802968.67790-1.3424075.5483073.85170HepG2SRFBP1
ACO2ENSG00000100412.112166.959303283.76280-0.5997017.9065016.92320HepG2SRSF1
ACO2ENSG00000100412.112438.597401964.831100.311504.517903.66130HepG2SRSF7
ACO2ENSG00000100412.113162.189602577.574800.294805.082704.42910HepG2SSB
ACO2ENSG00000100412.111990.839602630.73380-0.402102.674801.41210HepG2SSRP1
ACO2ENSG00000100412.112559.881101719.700800.573808.267106.70610HepG2SUPT6H
ACO2ENSG00000100412.111088.986101471.43630-0.434203.217702.49480HepG2SUPV3L1
ACO2ENSG00000100412.111535.218102951.75160-0.9431029.6573028.07890HepG2TAF15
ACO2ENSG00000100412.111851.294501434.233000.368203.065302.30730HepG2TARDBP
ACO2ENSG00000100412.111982.138301377.028200.5254011.2958010.27000HepG2TRIM56
ACO2ENSG00000100412.111646.273202178.94820-0.404403.968503.22230HepG2U2AF1
ACO2ENSG00000100412.111778.072002322.81550-0.385607.225806.18580HepG2UPF1
ACO2ENSG00000100412.111672.013702242.58570-0.423607.658406.74290HepG2UTP3
ACO2ENSG00000100412.113867.809602554.651100.5983024.4673023.24650HepG2ZNF622
ACO2ENSG00000100412.115897.697104799.241700.297205.850605.07410K562AGGF1
ACO2ENSG00000100412.115969.878504993.833900.257502.303901.70660K562AKAP8L
ACO2ENSG00000100412.112702.627604727.25820-0.8066014.5359013.33920K562AQR
ACO2ENSG00000100412.111393.409001902.73610-0.449504.186103.10640K562BOP1
ACO2ENSG00000100412.111676.201502183.20770-0.381303.371802.18440K562CCAR1
ACO2ENSG00000100412.116484.649407660.23250-0.240402.627701.67790K562CELF1
ACO2ENSG00000100412.114043.133604733.00180-0.227303.217102.52250K562DDX47
ACO2ENSG00000100412.111637.414602444.14080-0.577906.454504.30930K562DHX30
ACO2ENSG00000100412.116290.268405373.689400.227103.022602.10070K562DKC1
ACO2ENSG00000100412.111713.758202347.66980-0.454104.682402.91280K562DNAJC21
ACO2ENSG00000100412.113078.146803833.45340-0.316604.938004.01670K562EIF2S2
ACO2ENSG00000100412.111898.407701366.489500.474207.114005.85080K562EIF3G
ACO2ENSG00000100412.111908.975102422.54220-0.343702.582401.50000K562FKBP4
ACO2ENSG00000100412.111466.872002058.52130-0.488905.182804.00690K562FMR1
ACO2ENSG00000100412.111381.348001744.79140-0.337002.255801.43490K562FTO
ACO2ENSG00000100412.113020.209002664.102200.180901.850201.33540K562FXR1
ACO2ENSG00000100412.112443.823301975.853400.306602.839101.38710K562G3BP1
ACO2ENSG00000100412.11497.800001310.98410-1.3970018.3558016.08260K562G3BP2
ACO2ENSG00000100412.111332.196201931.59600-0.536006.566705.34730K562GPKOW
ACO2ENSG00000100412.113598.791805108.10730-0.505308.180306.86760K562GRSF1
ACO2ENSG00000100412.112068.124402788.96200-0.431405.466404.65560K562HNRNPC
ACO2ENSG00000100412.111401.367402063.74790-0.558404.440602.84480K562HNRNPK
ACO2ENSG00000100412.112769.846504587.63300-0.7279020.3162018.02860K562HNRNPU
ACO2ENSG00000100412.113223.955702067.639000.640705.585703.91390K562IGF2BP2
ACO2ENSG00000100412.112707.284102102.632900.364502.883601.66260K562IGF2BP3
ACO2ENSG00000100412.111517.155202204.23270-0.538905.618303.51310K562KHDRBS1
ACO2ENSG00000100412.11972.793001649.36430-0.761707.245905.65120K562KRR1
ACO2ENSG00000100412.113017.307502167.358800.477206.599505.08920K562MATR3
ACO2ENSG00000100412.113283.419004123.45920-0.328706.727405.51600K562MTPAP
ACO2ENSG00000100412.115422.007406814.62560-0.329803.231101.60180K562NOL12
ACO2ENSG00000100412.113871.187304581.54560-0.243105.909404.87580K562NSUN2
ACO2ENSG00000100412.115059.243002914.226500.795704.357803.04120K562PABPN1
ACO2ENSG00000100412.112588.615602123.563000.285602.792001.43740K562PCBP1
ACO2ENSG00000100412.111395.606101932.28210-0.469404.630603.36000K562PCBP2
ACO2ENSG00000100412.113219.576504352.66400-0.435004.046003.04040K562PCBP3
ACO2ENSG00000100412.112705.637103338.55980-0.303303.498302.59090K562POLR2G
ACO2ENSG00000100412.114544.549806797.52500-0.580908.812507.34150K562PUF60
ACO2ENSG00000100412.115802.410207230.10630-0.317404.213602.87770K562PUM1
ACO2ENSG00000100412.115494.012407084.20340-0.366706.832705.30310K562PUM2
ACO2ENSG00000100412.115641.457204825.472300.225302.698902.11180K562RBM15
ACO2ENSG00000100412.111216.617901837.04720-0.594505.571404.15760K562SF3B1
ACO2ENSG00000100412.112726.267702068.865300.398004.492903.59600K562SRSF1
ACO2ENSG00000100412.112807.298801954.187000.522504.810703.35680K562SRSF5
ACO2ENSG00000100412.112833.625602019.549200.488504.591403.49690K562TIA1
ACO2ENSG00000100412.112946.334101982.395800.571604.574703.10870K562TRA2A
ACO2ENSG00000100412.11842.920601270.00720-0.591404.545103.07820K562U2AF1
ACO2ENSG00000100412.11578.251001311.89050-1.1819024.2021021.82820K562U2AF2
ACO2ENSG00000100412.113595.365104857.90900-0.4342011.5724010.22950K562UPF1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for ACO2 is not available on ENCORE database.





1 RBPs can affect the splicing of ACO2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A5SSACO2ENSG00000100412.11chr22+4191174741911996419117474191192141913530419136350.075000.000000.00000longerK562AQR
A5SSACO2ENSG00000100412.11chr22+4191617441916381419161744191628041918833419189910.067000.000000.00000longerK562AQR
MXEACO2ENSG00000100412.11chr22+41911747419119214191353041913635419113804191153941914484419145760.057000.000010.000111includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for ACO2 is not avaliable on ENCORE database.