ZNF335

General Information

Gene Name       : ZNF335
Gene ID             : ENSG00000198026.7
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: ZNF335)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ZNF335ENSG00000198026.72.178.495.33ENCSR000AEF
HepG2ZNF335ENSG00000198026.713.895.779.83ENCSR985KAT
K562ZNF335ENSG00000198026.79.096.087.58ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ZNF335-BGKcLV17-K562ENCSR650GNBZNF335K562ReleasedENCSR516TLX   CRISPRKD% = 51.2,59.6






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ZNF335ENCAB644YFOPA5-67158UC2731469ANotSatisfied, ENCODE4BGKcLV17-57, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ZNF335K562CRISPRBGKcLV17-55,BGKcLV17-56BGKcLV17-1,BGKcLV17-2NotSatisfiedENCODE4
ZNF335K562CRISPRENCSR650GNBENCSR516TLXZNF335-BGKcLV17-57,ZNF335-BGKcLV17-58NT-BGKcLV17-1,NT-BGKcLV17-251.2,59.6ReleasedENCODE4
ZNF335HepG2CRISPRBGHcLV18-51,BGHcLV18-52BGHcLV18-1,BGHcLV18-2NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ZNF335Product_ID: PA5-67158
Lot_ID: UC2731469A
Source: Thermo Fisher
Target Name: ZNF335-human		 ThermoFisher_PA5-67158_UC2731469A_ZNF335.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ZNF335 specific antibody, PA5-67158. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the ZNF335-specific antibody, PA5-67158. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ZNF335-K562-CRISPR-PA5-67158.png<br>Caption: Western blot following CRISPR against ZNF335 in K562 whole cell lysate using ZNF335 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZNF335. ZNF335 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ZNF335 is not avaliable on ENCORE database.



Proteins bound to RNA
  • ZNF335 RNA is bound by 11 proteins.  







eCLIP assay for ZNF335 is not avaliable on ENCORE database.





11 proteins are bound to ZNF335 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr204457741344577490-3'-UTR,intron-Distal3.246293.17522HepG2LSM11
chr204457740144577505-3'-UTR,intron-Distal4.506473.99014HepG2PABPN1
chr204459997944600120-5'-UTR,CDS,intron-Distal,intron-Proximal4.101843.61584HepG2SRSF1
chr204458948744589566-CDS,intron-Distal,intron-Proximal4.945565.26064K562AQR
chr204457895544579012-CDS,intron-Distal,intron-Proximal4.094023.19682HepG2PABPN1
chr204458074744580826-CDS,intron-Distal,intron-Proximal4.142479.08333HepG2PRPF8
chr204457860344578700-CDS,intron-Distal,intron-Proximal4.698316.66265HepG2PABPN1
chr204458904644589107-CDS,intron-Distal,intron-Proximal5.092276.01989HepG2PRPF8
chr204458119344581257-CDS,intron-Distal,intron-Proximal4.141266.23016HepG2PRPF8
chr204458629044586388-CDS,intron-Distal,intron-Proximal3.167508.45537HepG2PRPF8
chr204459979744599857-CDS,intron-Distal,intron-Proximal3.092126.67352HepG2PRPF8
chr204457784644577891-CDS,intron-Distal,intron-Proximal4.824266.16445HepG2PPIG
chr204458630244586372-CDS,intron-Distal,intron-Proximal3.702925.51372HepG2EFTUD2
chr204458884844588883-CDS,intron-Distal4.398694.38106HepG2PPIG
chr204458085644580913-CDS,intron-Distal3.477524.77136HepG2PPIG
chr204459828544598356-CDS,intron-Distal,intron-Proximal3.392943.50696K562BUD13
chr204459260944592686-CDS,intron-Distal,intron-Proximal4.422564.59919HepG2AQR
chr204459255744592660-CDS,intron-Distal,intron-Proximal4.254963.70678K562BUD13
chr204459256344592627-CDS,intron-Distal4.135665.80295HepG2PPIG
chr204459260944592653-CDS,intron-Distal,intron-Proximal4.264157.23600HepG2SF3B4
chr204458250644582565-CDS,intron-Distal4.303726.92541HepG2PPIG
chr204459434744594404-CDS,intron-Distal3.142424.94718HepG2PPIG
chr204458618444586256-CDS,intron-Distal,intron-Proximal4.078729.97647HepG2PRPF8
chr204459207444592139-CDS,intron-Distal,intron-Proximal3.580486.18341HepG2PRPF8
chr204458892744588988-CDS,intron-Distal3.969143.02457HepG2BCLAF1
chr204457781644577891-CDS,intron-Distal,intron-Proximal3.427313.77473HepG2PABPN1
chr204458256544582649-CDS,intron-Distal,intron-Proximal4.472877.39218K562SF3B4
chr204458947844589566-CDS,intron-Distal,intron-Proximal5.8101512.86303HepG2AQR
chr204457797144577993-CDS,intron-Distal,intron-Proximal4.439934.67689HepG2PPIG
chr204457843544578508-CDS,intron-Distal,intron-Proximal3.817266.70085HepG2PPIG
chr204459426744594277-CDS,intron-Distal,intron-Proximal4.297644.16554HepG2PPIG
chr204458078744580926-CDS,intron-Distal4.906826.39410HepG2PABPN1
chr204458936944589449-CDS,intron-Distal,intron-Proximal4.784595.26064K562AQR
chr204458952344589586-intron-Distal,intron-Proximal3.448273.59805HepG2SF3B4
chr204460036144600494-intron-Distal3.412305.13820HepG2NCBP2
chr204459265344592715-intron-Distal,intron-Proximal5.217558.89842HepG2SF3B4
chr204459419044594250-intron-Distal,intron-Proximal4.827995.68502HepG2PRPF8
chr204459413844594235-intron-Distal,intron-Proximal3.184883.18246K562PRPF8
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for ZNF335 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ZNF335 is not available on ENCORE database.

  • 101 RBPs can affect the levels of ZNF335.  








The Knockdown/knockout RNA-Seq assay for ZNF335 is not available on ENCORE database.





101 RBPs can affect the gene expression of ZNF335 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
ZNF335ENSG00000198026.6816.01060576.920400.500102.977301.99730HepG2ABCF1
ZNF335ENSG00000198026.6690.04180485.580500.506905.865305.04440HepG2ACO1
ZNF335ENSG00000198026.6621.53060411.937900.593302.679602.15610HepG2AKAP1
ZNF335ENSG00000198026.6600.91550406.366700.564302.584001.68080HepG2BCCIP
ZNF335ENSG00000198026.6481.70770323.813400.572903.542702.67510HepG2CIRBP
ZNF335ENSG00000198026.6713.11620485.461100.554707.250406.26820HepG2CKAP4
ZNF335ENSG00000198026.6374.88810276.325400.440002.688801.68690HepG2YBX3
ZNF335ENSG00000198026.6191.50740304.51000-0.669103.051401.60090HepG2CSTF2T
ZNF335ENSG00000198026.6518.86860339.035200.613803.720303.01340HepG2DDX6
ZNF335ENSG00000198026.6578.30530464.565300.315802.145501.53710HepG2DKC1
ZNF335ENSG00000198026.6438.62800268.944700.705607.822906.79770HepG2DNAJC2
ZNF335ENSG00000198026.6628.59520377.044200.737303.518402.48460HepG2EEF2
ZNF335ENSG00000198026.6615.08570325.827400.916608.638907.62760HepG2EIF2S1
ZNF335ENSG00000198026.6729.49280360.031401.0187010.174008.96610HepG2EIF4A3
ZNF335ENSG00000198026.6610.14340350.629200.799105.187104.26470HepG2EIF4B
ZNF335ENSG00000198026.61146.78870793.355900.531506.312205.46620HepG2ETF1
ZNF335ENSG00000198026.6626.25710474.486600.400302.354201.58430HepG2EWSR1
ZNF335ENSG00000198026.6453.20000282.643600.681105.081404.02800HepG2FUBP3
ZNF335ENSG00000198026.6592.97920363.396300.706305.445304.49660HepG2FXR1
ZNF335ENSG00000198026.6729.93350337.029901.1148012.7501011.37530HepG2G3BP1
ZNF335ENSG00000198026.6541.24810357.896400.596604.007102.92710HepG2G3BP2
ZNF335ENSG00000198026.6494.87070272.434000.861006.311604.96210HepG2GEMIN5
ZNF335ENSG00000198026.6454.90710321.747700.499502.241901.32910HepG2GNB2L1
ZNF335ENSG00000198026.6478.00810273.909300.803206.631205.69520HepG2HNRNPA1
ZNF335ENSG00000198026.6458.42280304.683600.589303.846603.04010HepG2HNRNPC
ZNF335ENSG00000198026.6684.89450546.064400.326701.906501.30370HepG2HNRNPF
ZNF335ENSG00000198026.6841.04310341.444801.3004019.4780017.77690HepG2HNRNPK
ZNF335ENSG00000198026.6679.61080462.182100.556107.248406.20350HepG2HNRNPL
ZNF335ENSG00000198026.6595.64730302.668500.9766015.6226014.27900HepG2HNRNPU
ZNF335ENSG00000198026.6674.07040537.717600.326002.185301.53770HepG2IGF2BP1
ZNF335ENSG00000198026.6649.72690342.557400.923409.036007.89430HepG2IGF2BP2
ZNF335ENSG00000198026.6528.41260321.580600.716402.408201.49830HepG2IGF2BP3
ZNF335ENSG00000198026.6397.85900309.164100.363802.585801.87500HepG2ILF2
ZNF335ENSG00000198026.6544.10940344.603000.658903.843002.64170HepG2KHSRP
ZNF335ENSG00000198026.6582.48410443.872900.392003.597602.43360HepG2KIF1C
ZNF335ENSG00000198026.6997.89150594.553900.747006.142404.89650HepG2LARP4
ZNF335ENSG00000198026.6795.96000363.523801.1305013.7760012.35790HepG2MAGOH
ZNF335ENSG00000198026.6511.91620365.535600.485802.471501.87200HepG2MATR3
ZNF335ENSG00000198026.6728.54420374.127600.961409.607308.46710HepG2NCBP2
ZNF335ENSG00000198026.6674.65930503.141000.423103.742602.85310HepG2NOL12
ZNF335ENSG00000198026.6252.60530353.39970-0.484402.285101.60260HepG2NSUN2
ZNF335ENSG00000198026.6542.44670346.771100.645403.043102.10960HepG2PABPC1
ZNF335ENSG00000198026.6652.31280338.067400.9482015.1064013.87110HepG2PCBP1
ZNF335ENSG00000198026.6459.20960309.097800.571002.820901.77050HepG2PCBP2
ZNF335ENSG00000198026.6423.88990269.954300.650903.726202.88290HepG2PPIL4
ZNF335ENSG00000198026.6594.25180344.003000.7886010.066509.15030HepG2PTBP1
ZNF335ENSG00000198026.6882.07050509.894100.7906016.8090015.60280HepG2PUM1
ZNF335ENSG00000198026.6850.57810509.507700.739209.998908.76670HepG2PUM2
ZNF335ENSG00000198026.6783.97670494.593200.664508.088507.12860HepG2PUS1
ZNF335ENSG00000198026.6504.02220338.117900.575904.406803.57170HepG2RBM25
ZNF335ENSG00000198026.6635.33890380.228800.740605.631704.20200HepG2RBM27
ZNF335ENSG00000198026.6519.10650308.764100.749404.201902.89080HepG2RCC2
ZNF335ENSG00000198026.6685.86310382.836700.8411011.3628010.40910HepG2NELFE
ZNF335ENSG00000198026.6585.49520356.570500.715408.178207.20230HepG2RECQL
ZNF335ENSG00000198026.6767.61670588.245100.383902.700802.02390HepG2RPLP0
ZNF335ENSG00000198026.6490.20190297.826700.718803.530102.84400HepG2RPS19
ZNF335ENSG00000198026.6535.98440350.643600.612103.816002.88020HepG2RPS2
ZNF335ENSG00000198026.6412.49480269.533700.613804.086803.33030HepG2SAFB2
ZNF335ENSG00000198026.61418.62890681.076501.058503.519802.75590HepG2SF3A3
ZNF335ENSG00000198026.6524.93510394.468600.412102.399801.74070HepG2SF3B1
ZNF335ENSG00000198026.6601.76500358.139400.748608.860307.89340HepG2SF3B4
ZNF335ENSG00000198026.6607.09700460.589700.398303.863803.05590HepG2SLBP
ZNF335ENSG00000198026.6448.49310314.311700.512804.533603.74520HepG2SMN1
ZNF335ENSG00000198026.6522.17700304.550900.777805.350203.98900HepG2SMNDC1
ZNF335ENSG00000198026.6992.25680748.892200.405903.827603.26760HepG2SRFBP1
ZNF335ENSG00000198026.6554.19820373.825000.567905.319504.71800HepG2SRSF1
ZNF335ENSG00000198026.6715.50850474.264300.593207.586106.61760HepG2SRSF5
ZNF335ENSG00000198026.6682.37450536.490200.346902.841802.13720HepG2SRSF7
ZNF335ENSG00000198026.6422.35980304.130800.473703.751203.13050HepG2SRSF9
ZNF335ENSG00000198026.6971.14850650.158800.578808.270707.47640HepG2SSB
ZNF335ENSG00000198026.6859.13770539.346200.6716011.2109010.10340HepG2SUCLG1
ZNF335ENSG00000198026.6406.40400308.744000.396402.316201.54900HepG2TIA1
ZNF335ENSG00000198026.6590.68070294.060801.0062013.5294012.56600HepG2TIAL1
ZNF335ENSG00000198026.6433.40060296.730500.546404.652803.95970HepG2TRIM56
ZNF335ENSG00000198026.6868.78740469.280400.888504.684403.51430HepG2TUFM
ZNF335ENSG00000198026.6989.47660498.196700.9899012.5671011.19530HepG2U2AF1
ZNF335ENSG00000198026.6570.31310216.611101.396504.863503.52570HepG2U2AF2
ZNF335ENSG00000198026.6333.05000234.904800.503602.142801.47490HepG2UCHL5
ZNF335ENSG00000198026.6773.54300604.010500.356803.413802.65180HepG2UPF1
ZNF335ENSG00000198026.6823.99900583.148700.498705.820605.00500HepG2UTP3
ZNF335ENSG00000198026.6495.26070328.024300.594303.158702.02280HepG2XRCC6
ZNF335ENSG00000198026.6664.72280391.511500.763605.264904.27270HepG2XRN2
ZNF335ENSG00000198026.6789.72430664.296200.249402.153701.65360HepG2ZNF622
ZNF335ENSG00000198026.61052.63280730.151000.527604.955904.21160K562AQR
ZNF335ENSG00000198026.6680.68410341.756300.993905.979104.59750K562EFTUD2
ZNF335ENSG00000198026.6559.78050440.384100.346002.630801.45870K562EIF2S1
ZNF335ENSG00000198026.6292.30080184.319300.665104.232403.25750K562EIF3G
ZNF335ENSG00000198026.6494.22720377.217700.389702.789401.62940K562EIF4B
ZNF335ENSG00000198026.6576.02200435.615500.403003.068802.43590K562FXR1
ZNF335ENSG00000198026.644.86610142.38140-1.666102.999302.25850K562G3BP2
ZNF335ENSG00000198026.6374.37190280.486000.416402.095001.39980K562GPKOW
ZNF335ENSG00000198026.6467.36380265.791300.814205.689303.99330K562IGF2BP2
ZNF335ENSG00000198026.6388.49490270.311100.523203.390402.03590K562IGF2BP3
ZNF335ENSG00000198026.6392.85710279.250600.492303.062902.17720K562MAGOH
ZNF335ENSG00000198026.6678.91960378.535200.842703.860602.63760K562PABPN1
ZNF335ENSG00000198026.6370.63440248.448300.577003.390002.30850K562PCBP2
ZNF335ENSG00000198026.6433.45890717.73530-0.727606.165004.91920K562PCBP3
ZNF335ENSG00000198026.6348.58460550.53430-0.659306.287005.08350K562POLR2G
ZNF335ENSG00000198026.6773.95160450.014500.782203.176301.57130K562PPP1R8
ZNF335ENSG00000198026.6616.74290332.572800.890907.933806.65610K562PRPF8
ZNF335ENSG00000198026.6497.74720271.315900.875309.689508.50170K562PTBP1
ZNF335ENSG00000198026.6900.504701117.21490-0.311102.357201.37890K562PUM1
ZNF335ENSG00000198026.6914.45940742.488500.300502.393301.83530K562RBM15
ZNF335ENSG00000198026.6477.35870260.112800.875808.156706.60830K562RBM22
ZNF335ENSG00000198026.6364.28190253.596100.522402.882501.46970K562RBM34
ZNF335ENSG00000198026.6479.74140355.545300.432102.620901.65810K562RCC2
ZNF335ENSG00000198026.6639.59850490.517700.382802.477501.45580K562SAFB
ZNF335ENSG00000198026.6677.96760346.167400.969603.692202.23850K562SF1
ZNF335ENSG00000198026.6288.59870147.761900.965704.049203.00760K562SF3B4
ZNF335ENSG00000198026.6733.67140421.997900.797803.274801.81080K562SFPQ
ZNF335ENSG00000198026.6661.48710397.571900.734402.753801.52720K562SNRNP200
ZNF335ENSG00000198026.6431.07150265.989600.696505.401904.42020K562SRSF1
ZNF335ENSG00000198026.6437.93490251.230900.801602.434201.45840K562SRSF5
ZNF335ENSG00000198026.6619.85220269.178101.203306.150205.06050K562TARDBP
ZNF335ENSG00000198026.6496.77420259.658100.9359010.160308.47770K562TIA1
ZNF335ENSG00000198026.6467.77720254.850900.876107.888005.78930K562TRA2A
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for ZNF335 is not available on ENCORE database.





2 RBPs can affect the splicing of ZNF335 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEZNF335ENSG00000198026.6chr20-4457936644579418445790914457923444580785445811600.195000.000630.00826includedK562AQR
SEZNF335ENSG00000198026.6chr20-445862244458631944582327445825874458647344586693-0.080000.005390.04942excludedK562AQR
A3SSZNF335ENSG00000198026.6chr20-4457797144578064445779714457803744578123445782070.200000.000620.08836longerK562RBM22
A3SSZNF335ENSG00000198026.6chr20-4457797144578064445779714457803744578123445782070.164000.000020.00342longerHepG2RBM22
A3SSZNF335ENSG00000198026.6chr20-4457860444578751445786044457870844578857445790120.171000.000170.00143longerK562AQR
A3SSZNF335ENSG00000198026.6chr20-4459070844590889445907084459082144592111445923170.183000.000320.00252longerK562AQR
A3SSZNF335ENSG00000198026.6chr20-4458622444586360445862244458631944586473445865920.151000.000030.00026longerK562AQR
A3SSZNF335ENSG00000198026.6chr20-4457909144579264445790914457923444580785445811600.251000.000790.00566longerK562AQR
A3SSZNF335ENSG00000198026.6chr20-4457812344578291445781234457820744578438445785160.145000.000000.00003longerK562AQR
A3SSZNF335ENSG00000198026.6chr20-4458123644581433445812364458134844582327445825870.323000.000000.00001longerK562AQR
A3SSZNF335ENSG00000198026.6chr20-4458647344586693445864734458656744587839445880720.285000.000000.00000longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for ZNF335 is not avaliable on ENCORE database.