ADAT1

General Information

Gene Name       : ADAT1
Gene ID             : ENSG00000065457.10
Essential Gene : No

Functions

Localization

Domains (AlphaFold: ADAT1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ADAT1ENSG00000065457.1054.7152.7053.70ENCSR000AEF
HepG2ADAT1ENSG00000065457.1029.1430.7329.94ENCSR985KAT
K562ADAT1ENSG00000065457.1028.4324.1826.30ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
eCLIPeCLIP: 4217ENCSR750YOMADAT1K562ReleasedENCAB109YPJENCSR546JKS  
knockdown followed by RNA-seq ADAT1-BGKcLV26-K562ENCSR122RJEADAT1K562ReleasedENCSR900GQL   CRISPRKD% = 70.3,74.4
knockdown followed by RNA-seq ADAT1-BGHcLV25-HepG2ENCSR051LWNADAT1HepG2ReleasedENCSR953VMB   CRISPRKD% = 60.2,59.4






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
ADAT14217K562ENCSR750YOMENCSR546JKSENCAB109YPJPA5-98841UK2881574ReleasedENCODE4


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ADAT1ENCAB109YPJPA5-98841UK2881574BGHcLV25-11, Released, ENCODE4BGKcLV26-7, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ADAT1K562CRISPRBGKcLV23-3,BGKcLV23-4BGKcLV23-1,BGKcLV23-20.0,0.0NotSatisfiedENCODE4
ADAT1K562CRISPRBGKcLV23-5,BGKcLV23-6BGKcLV23-1,BGKcLV23-20.0,0.0NotSatisfiedENCODE4
ADAT1K562CRISPRENCSR122RJEENCSR900GQLADAT1-BGKcLV26-7,ADAT1-BGKcLV26-8NT-BGKcLV26-1,NT-BGKcLV26-270.3,74.4ReleasedENCODE4
ADAT1HepG2CRISPRBGHcLV24-3,BGHcLV24-4BGHcLV24-1,BGHcLV24-224.0,58.0NotSatisfiedENCODE4
ADAT1HepG2CRISPRBGHcLV24-5,BGHcLV24-6BGHcLV24-1,BGHcLV24-229.0,43.0NotSatisfiedENCODE4
ADAT1HepG2CRISPRBGHcLV25-7,BGHcLV25-8BGHcLV25-1,BGHcLV25-210.8,21.2NotSatisfiedENCODE4
ADAT1HepG2CRISPRBGHcLV25-9,BGHcLV25-10BGHcLV25-1,BGHcLV25-218.7,39.1NotSatisfiedENCODE4
ADAT1HepG2CRISPRENCSR051LWNENCSR953VMBADAT1-BGHcLV25-11,ADAT1-BGHcLV25-12NT-BGHcLV25-1,NT-BGHcLV25-260.2,59.4ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ADAT1Product_ID: PA5-98841
Lot_ID: UK2881574
Source: Thermo Fisher
Target Name: ADAT1-human		 ADAT1-HEPG2-CRISPR-PA5-98841.png<br>Caption: Western blot following CRISPR against ADAT1 in HepG2 whole cell lysate using ADAT1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ADAT1. ADAT1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ADAT1-K562-CRISPR-PA5-98841.png<br>Caption: Western blot following CRISPR against ADAT1 in K562 whole cell lysate using ADAT1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ADAT1. ADAT1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ADAT1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • ADAT1 RNA is bound by 2 proteins.  







eCLIP assay for ADAT1 is not avaliable on ENCORE database.





2 proteins are bound to ADAT1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr167565464375654722-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal4.705184.67689HepG2PPIG
chr167565701775657138-5'-UTR,intron-Distal3.541904.35138HepG2NCBP2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for ADAT1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ADAT1 is not available on ENCORE database.

  • 100 RBPs can affect the levels of ADAT1.  








The Knockdown/knockout RNA-Seq assay for ADAT1 is not available on ENCORE database.





100 RBPs can affect the gene expression of ADAT1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
ADAT1ENSG00000065457.6340.81020225.450800.596103.356402.18230HepG2AATF
ADAT1ENSG00000065457.6485.48500342.597900.502802.590201.67860HepG2ABCF1
ADAT1ENSG00000065457.6419.60060289.565900.535004.459303.72340HepG2ACO1
ADAT1ENSG00000065457.6412.05610184.796201.156808.694507.81180HepG2AKAP1
ADAT1ENSG00000065457.6376.91590290.957300.373302.241901.54410HepG2AKAP8L
ADAT1ENSG00000065457.6243.63110145.274900.745803.682802.79990HepG2CIRBP
ADAT1ENSG00000065457.6336.45960180.821200.895804.517603.53710HepG2CNOT7
ADAT1ENSG00000065457.6233.02180351.78950-0.594203.274902.53280HepG2CPSF6
ADAT1ENSG00000065457.6466.31460306.512900.605305.449804.60330HepG2CELF1
ADAT1ENSG00000065457.6462.39810248.789000.894107.550506.48600HepG2DDX21
ADAT1ENSG00000065457.6338.16800177.042100.933506.249705.34610HepG2DDX3X
ADAT1ENSG00000065457.6319.98380180.706700.824204.561003.40130HepG2EEF2
ADAT1ENSG00000065457.6262.65460150.606700.802304.350702.95860HepG2EFTUD2
ADAT1ENSG00000065457.6265.07260146.669800.853704.495103.36520HepG2EIF3D
ADAT1ENSG00000065457.6304.63340161.594900.914605.598004.68940HepG2EIF4A3
ADAT1ENSG00000065457.6521.30150157.289401.7286017.4277015.80200HepG2EIF4B
ADAT1ENSG00000065457.6784.19510591.118100.407704.273703.55420HepG2ETF1
ADAT1ENSG00000065457.6416.98460282.990600.559103.856002.87000HepG2EWSR1
ADAT1ENSG00000065457.6599.15520345.955200.792205.859104.78100HepG2FIP1L1
ADAT1ENSG00000065457.6401.23980276.300200.538102.420701.37190HepG2FUS
ADAT1ENSG00000065457.6366.67880162.969801.169809.702708.47630HepG2FXR1
ADAT1ENSG00000065457.6348.34750151.216601.203809.952608.72880HepG2G3BP1
ADAT1ENSG00000065457.6262.20110160.485800.708103.256002.28630HepG2G3BP2
ADAT1ENSG00000065457.6199.42410130.583500.610802.520301.66990HepG2GEMIN5
ADAT1ENSG00000065457.6362.15820254.444200.509202.460801.50470HepG2GNB2L1
ADAT1ENSG00000065457.6335.69780518.88540-0.628306.333105.30900HepG2HNRNPF
ADAT1ENSG00000065457.6222.07030153.031200.537102.064101.45520HepG2HNRNPK
ADAT1ENSG00000065457.6204.88480380.54380-0.893204.670503.87370HepG2HNRNPLL
ADAT1ENSG00000065457.6361.47590238.731800.598403.920902.80840HepG2HNRNPM
ADAT1ENSG00000065457.6323.60150153.722001.073808.010806.92450HepG2IGF2BP2
ADAT1ENSG00000065457.6311.52500144.206601.111103.960602.78480HepG2IGF2BP3
ADAT1ENSG00000065457.6251.61610154.592600.702702.835601.82000HepG2KHSRP
ADAT1ENSG00000065457.6352.48430249.318400.499502.496601.42360HepG2LARP7
ADAT1ENSG00000065457.6461.03310163.026301.4997016.4852014.95240HepG2MAGOH
ADAT1ENSG00000065457.6435.98960163.950601.4109013.1507011.85010HepG2MATR3
ADAT1ENSG00000065457.6317.96080153.486401.050604.903503.52810HepG2METAP2
ADAT1ENSG00000065457.6436.99380280.053100.641804.668503.63820HepG2MSI2
ADAT1ENSG00000065457.6575.01950167.822201.7766023.7993022.06450HepG2NCBP2
ADAT1ENSG00000065457.6207.03980337.44180-0.704704.012003.11590HepG2NSUN2
ADAT1ENSG00000065457.6265.48440149.500800.828404.663003.73690HepG2PABPC4
ADAT1ENSG00000065457.6353.93470258.746600.451802.810601.95620HepG2PARN
ADAT1ENSG00000065457.6307.73980163.566000.911706.186705.33450HepG2PCBP1
ADAT1ENSG00000065457.6198.64400121.296600.711503.409502.04420HepG2PKM
ADAT1ENSG00000065457.6435.89360274.991700.664503.337402.00710HepG2PRPF6
ADAT1ENSG00000065457.6473.15500166.415901.5074023.0307021.74300HepG2PTBP1
ADAT1ENSG00000065457.6767.42840331.166801.2124029.3114027.79480HepG2PUM1
ADAT1ENSG00000065457.6473.43830294.947100.682607.269206.34960HepG2PUS1
ADAT1ENSG00000065457.6372.94410180.272301.048703.992903.26170HepG2QKI
ADAT1ENSG00000065457.6687.62740494.065300.476804.564803.94300HepG2RBM22
ADAT1ENSG00000065457.6309.83840163.593700.921304.226203.40800HepG2RBM25
ADAT1ENSG00000065457.6438.81620185.220401.2443014.6849013.62450HepG2NELFE
ADAT1ENSG00000065457.6299.18480172.479600.794505.442304.61450HepG2RECQL
ADAT1ENSG00000065457.6376.49380142.740001.3991014.6216013.35770HepG2RPS19
ADAT1ENSG00000065457.6482.37910277.262000.798805.971404.81970HepG2RPS2
ADAT1ENSG00000065457.6270.59410143.224200.917805.479504.16710HepG2SERBP1
ADAT1ENSG00000065457.6263.80030376.64980-0.513802.796702.08620HepG2SF3B1
ADAT1ENSG00000065457.6356.36360173.293901.040009.566708.56690HepG2SF3B4
ADAT1ENSG00000065457.6434.81760271.479800.679505.671204.80260HepG2SMN1
ADAT1ENSG00000065457.6397.16130180.867701.1347011.9405011.10530HepG2SRSF1
ADAT1ENSG00000065457.6545.50200308.021800.8245010.345109.23700HepG2SRSF5
ADAT1ENSG00000065457.6331.59970509.78180-0.620406.025605.06120HepG2SRSF7
ADAT1ENSG00000065457.6268.36300147.133700.867006.598305.81160HepG2SRSF9
ADAT1ENSG00000065457.6758.32640350.306401.1141016.3252015.01800HepG2SUCLG1
ADAT1ENSG00000065457.6335.90720162.533001.047209.602608.65970HepG2TAF15
ADAT1ENSG00000065457.6238.51580149.385900.674902.577001.76780HepG2TIA1
ADAT1ENSG00000065457.6331.86000254.029900.385501.952001.51690HepG2TIAL1
ADAT1ENSG00000065457.6205.31170116.531100.817003.735901.92350HepG2TROVE2
ADAT1ENSG00000065457.6450.03930304.778100.562203.931702.86710HepG2TUFM
ADAT1ENSG00000065457.6485.67800322.602000.590103.057902.17990HepG2UBE2L3
ADAT1ENSG00000065457.6191.24990305.93700-0.677802.288801.38230HepG2UPF2
ADAT1ENSG00000065457.6529.02280307.473100.782809.097808.11430HepG2UTP3
ADAT1ENSG00000065457.6189.62950328.76630-0.793903.225402.44530HepG2XRN2
ADAT1ENSG00000065457.6107.61030357.90630-1.7338020.8538019.41070K562AGGF1
ADAT1ENSG00000065457.6236.70690393.24020-0.732304.123502.87080K562AKAP8
ADAT1ENSG00000065457.6140.46580372.40670-1.4067015.1388013.40530K562AKAP8L
ADAT1ENSG00000065457.6264.12690391.12540-0.566403.280302.65140K562AQR
ADAT1ENSG00000065457.6235.14130390.72350-0.732604.407803.22590K562ATP5C1
ADAT1ENSG00000065457.6276.95150419.92120-0.600504.288602.44020K562DDX19B
ADAT1ENSG00000065457.6600.33000350.556000.7760010.161809.00320K562DDX47
ADAT1ENSG00000065457.6279.80160400.80530-0.518503.971802.89700K562DKC1
ADAT1ENSG00000065457.6489.35260307.787900.668806.581305.54130K562EIF2S2
ADAT1ENSG00000065457.6263.51910126.301601.060904.915403.53320K562EIF3A
ADAT1ENSG00000065457.6351.30260220.169800.674004.482603.48060K562EIF3G
ADAT1ENSG00000065457.6212.38430136.552900.637102.488801.71020K562EIF4A3
ADAT1ENSG00000065457.6595.86800461.652600.368102.668301.74390K562EIF4G2
ADAT1ENSG00000065457.6282.69940142.007300.993207.694906.75300K562FXR1
ADAT1ENSG00000065457.6284.18060410.19930-0.529502.898402.12640K562GRSF1
ADAT1ENSG00000065457.6117.77080256.70470-1.124103.024301.73150K562HNRNPA2B1
ADAT1ENSG00000065457.6217.56120395.25520-0.861407.038305.57740K562KIF1C
ADAT1ENSG00000065457.6223.78790378.32520-0.757504.904903.34550K562MAK16
ADAT1ENSG00000065457.6242.53790442.78030-0.868408.250606.51070K562MBNL1
ADAT1ENSG00000065457.6134.98690310.35350-1.201107.640905.88810K562NAA15
ADAT1ENSG00000065457.698.33240224.69910-1.192303.161702.08380K562PABPN1
ADAT1ENSG00000065457.6711.98260284.427501.3237016.6664014.68410K562PCBP3
ADAT1ENSG00000065457.6626.45700218.161201.5217026.5524024.30260K562POLR2G
ADAT1ENSG00000065457.6246.31180361.47300-0.553403.277102.37060K562PPIG
ADAT1ENSG00000065457.6124.67900196.14800-0.653702.289801.47460K562PSIP1
ADAT1ENSG00000065457.6137.73370369.40400-1.4233013.5288012.37410K562PUS1
ADAT1ENSG00000065457.6144.66560359.86330-1.3147014.3313012.97300K562RBM15
ADAT1ENSG00000065457.6357.42930219.022300.706504.470003.04600K562NELFE
ADAT1ENSG00000065457.6213.51450115.575200.885403.555802.40730K562RPS3
ADAT1ENSG00000065457.6114.62470235.95990-1.041602.738901.51770K562SNRNP200
ADAT1ENSG00000065457.6106.88220169.68150-0.666802.477101.51850K562SRFBP1
ADAT1ENSG00000065457.6167.77690374.45750-1.158306.350404.20150K562UTP18
ADAT1ENSG00000065457.6318.32690440.67250-0.469203.015602.10940K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for ADAT1 is not available on ENCORE database.





1 RBPs can affect the splicing of ADAT1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEADAT1ENSG00000065457.6chr16-756524837565253875651134756511707565416375654232-0.353000.002300.02466excludedK562AQR
A3SSADAT1ENSG00000065457.6chr16-7564668575646777756466857564675975651039756511700.138000.000260.00210longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for ADAT1 is not avaliable on ENCORE database.