DDX50

General Information

Gene Name       : DDX50
Gene ID             : ENSG00000107625.12
Essential Gene : No

Functions

Localization

Domains (AlphaFold: DDX50)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DDX50ENSG00000107625.1235.7132.4234.06ENCSR000AEF
HepG2DDX50ENSG00000107625.1213.8323.1918.51ENCSR985KAT
K562DDX50ENSG00000107625.1244.8349.0046.91ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DDX50ENCAB756NJZA301-312A1BGKcLV37-11, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DDX50K562CRISPRDDX50-BGKcLV37-11,DDX50-BGKcLV37-12NT-BGKcLV37-1,NT-BGKcLV37-256.0,54.2SubmittingENCORE2
DDX50HepG2CRISPRBGHcLV39-7,BGHcLV39-8BGHcLV39-1,BGHcLV39-2NO BAND,NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX50Product_ID: A301-312A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX50-human		 DDX50-K562-CRISPR-A301-312A.png<br>Caption: Western blot following CRISPR against DDX50 in K562 whole cell lysate using DDX50 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX50. DDX50 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for DDX50 is not avaliable on ENCORE database.



Proteins bound to RNA
  • DDX50 RNA is bound by 13 proteins.  







eCLIP assay for DDX50 is not avaliable on ENCORE database.





13 proteins are bound to DDX50 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr107070638670706438+3'-UTR4.460205.20653K562LIN28B
chr107070647870706568+3'-UTR5.902547.71888HepG2SUB1
chr107067011570670138+3'-UTR,CDS4.033664.32914K562YBX3
chr107070638170706504+3'-UTR,CDS4.9995851.00028K562PUM2
chr107066106870661223+5'-UTR,CDS3.4818013.34188K562DDX3X
chr107066108870661168+5'-UTR,CDS5.773226.24391K562GEMIN5
chr107066116470661223+CDS3.452705.65306K562ZNF622
chr107067970070679723+CDS4.903006.32937K562LIN28B
chr107067964770679700+CDS5.375568.98683K562LIN28B
chr107067329570673318+CDS4.381363.89826HepG2BCLAF1
chr107069458370694634+CDS,intron-Distal4.608794.56848K562PUM2
chr107066116670661221+CDS3.117364.30396K562UCHL5
chr107070200370702116+intron-Distal3.046343.46531K562KHSRP
chr107068911870689203+intron-Distal5.4311415.17671K562PTBP1
chr107068911370689216+intron-Distal3.603623.89405HepG2PTBP1
chr107068112570681272+intron-Distal3.881263.66668K562KHSRP
chr107069041370690440+intron-Distal4.305434.25105HepG2HNRNPC
chr107070067970700769+intron-Distal,intron-Proximal3.636143.06150K562KHSRP
chr107069044070690468+intron-Distal4.341504.43741HepG2HNRNPC
chr107066125470661300+intron-Proximal4.089654.34418HepG2RBM22
chr107066677870666842+intron-Proximal3.950013.66668K562KHSRP
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for DDX50 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for DDX50 is not available on ENCORE database.

  • 81 RBPs can affect the levels of DDX50.  








The Knockdown/knockout RNA-Seq assay for DDX50 is not available on ENCORE database.





81 RBPs can affect the gene expression of DDX50 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
DDX50ENSG00000107625.8688.38900498.134400.466603.469602.27800HepG2AATF
DDX50ENSG00000107625.8547.59650710.44530-0.375603.402602.56360HepG2AKAP8L
DDX50ENSG00000107625.8825.303901057.51100-0.357702.230401.52140HepG2ASCC1
DDX50ENSG00000107625.8552.755401004.70100-0.862109.926708.53980HepG2CIRBP
DDX50ENSG00000107625.8532.93330804.47770-0.594103.181002.35740HepG2CNOT7
DDX50ENSG00000107625.8377.73880500.72150-0.406602.783301.61900HepG2DDX1
DDX50ENSG00000107625.8579.874701224.33440-1.0782015.0598013.71420HepG2DDX3X
DDX50ENSG00000107625.8342.12940486.58880-0.508204.320003.53820HepG2DNAJC2
DDX50ENSG00000107625.8419.42410577.12060-0.460502.564301.67450HepG2EEF2
DDX50ENSG00000107625.8569.418901011.05570-0.828308.895807.87070HepG2EIF2S1
DDX50ENSG00000107625.8657.978501117.39000-0.764007.295706.25830HepG2EIF4A3
DDX50ENSG00000107625.8720.339601087.83180-0.594703.834203.02900HepG2EIF4B
DDX50ENSG00000107625.8503.80280690.98460-0.455803.619702.66390HepG2EWSR1
DDX50ENSG00000107625.8793.791701127.23460-0.506004.023603.19930HepG2FXR1
DDX50ENSG00000107625.8570.596701045.76220-0.874009.608508.40650HepG2G3BP1
DDX50ENSG00000107625.8759.994501110.09710-0.546604.728303.54980HepG2G3BP2
DDX50ENSG00000107625.8289.62080417.17590-0.526502.928202.00470HepG2GEMIN5
DDX50ENSG00000107625.8314.29990495.70550-0.657305.083004.24280HepG2HNRNPA1
DDX50ENSG00000107625.8837.019201058.72620-0.339002.225101.59380HepG2HNRNPK
DDX50ENSG00000107625.8446.08680576.09170-0.369001.940101.40450HepG2HNRNPLL
DDX50ENSG00000107625.8623.709401063.02530-0.769208.145807.05120HepG2IGF2BP2
DDX50ENSG00000107625.8426.66300559.47360-0.391002.717401.99080HepG2ILF2
DDX50ENSG00000107625.8775.597601069.16410-0.463102.677001.69350HepG2KHSRP
DDX50ENSG00000107625.8727.108501127.62740-0.633005.783004.84610HepG2MAGOH
DDX50ENSG00000107625.8672.486601133.96540-0.753807.152506.17250HepG2MATR3
DDX50ENSG00000107625.8294.19660490.38430-0.737102.670501.63620HepG2METAP2
DDX50ENSG00000107625.8464.934801160.69850-1.3199020.6048018.97990HepG2NCBP2
DDX50ENSG00000107625.8627.798301033.77800-0.719607.058605.91520HepG2PABPC4
DDX50ENSG00000107625.8769.41010932.38710-0.277202.506101.92160HepG2PCBP1
DDX50ENSG00000107625.8726.39550948.70890-0.385204.334203.68250HepG2PTBP1
DDX50ENSG00000107625.8590.14500905.28500-0.617304.792804.03410HepG2PUF60
DDX50ENSG00000107625.8757.73380961.06830-0.342902.737202.14690HepG2PUM1
DDX50ENSG00000107625.8684.90480960.32380-0.487604.590003.73730HepG2PUM2
DDX50ENSG00000107625.8859.911401027.53870-0.256901.963001.42280HepG2QKI
DDX50ENSG00000107625.8933.38010738.827200.337102.832401.96610HepG2RBM15
DDX50ENSG00000107625.8816.66140982.43970-0.266602.219501.74200HepG2RBM22
DDX50ENSG00000107625.8764.93100932.53340-0.285801.993501.41400HepG2RBM25
DDX50ENSG00000107625.8447.92460787.12150-0.813304.275403.02570HepG2RBM27
DDX50ENSG00000107625.8752.672301055.84670-0.488306.383705.61930HepG2NELFE
DDX50ENSG00000107625.8812.25410983.33280-0.275702.482001.89430HepG2RECQL
DDX50ENSG00000107625.8550.81940808.02680-0.552803.356102.47570HepG2RPS3A
DDX50ENSG00000107625.8800.72170987.78180-0.302902.888802.28720HepG2SF3B4
DDX50ENSG00000107625.8459.85190568.85560-0.306902.266901.67490HepG2SMN1
DDX50ENSG00000107625.8948.13850634.460900.579502.442601.71000HepG2SNRNP70
DDX50ENSG00000107625.8953.212101109.62080-0.219201.952101.50920HepG2SRFBP1
DDX50ENSG00000107625.8445.42540613.56800-0.462002.280001.38140HepG2SRSF3
DDX50ENSG00000107625.8681.11480893.91890-0.392203.280102.60840HepG2SRSF5
DDX50ENSG00000107625.8671.58230838.76160-0.320703.212202.62660HepG2SRSF9
DDX50ENSG00000107625.8418.79670561.54390-0.423102.529601.83200HepG2TARDBP
DDX50ENSG00000107625.8204.88780399.34880-0.962806.659205.22850HepG2TFIP11
DDX50ENSG00000107625.8390.83130536.95530-0.458304.117703.45740HepG2TRIM56
DDX50ENSG00000107625.8664.96910884.53670-0.411602.979102.06830HepG2TUFM
DDX50ENSG00000107625.8764.53060581.187600.395502.553601.94810HepG2U2AF1
DDX50ENSG00000107625.8219.69520351.97060-0.680003.808302.91820HepG2UCHL5
DDX50ENSG00000107625.81990.166002284.69410-0.199102.027601.52740K562AGGF1
DDX50ENSG00000107625.81909.302503471.06730-0.8623016.1151014.85690K562AQR
DDX50ENSG00000107625.81507.306101854.11550-0.298803.510201.98980K562BCCIP
DDX50ENSG00000107625.81538.534201791.78810-0.219802.410401.51630K562BCLAF1
DDX50ENSG00000107625.8464.54380641.57400-0.465803.046801.93290K562CCAR1
DDX50ENSG00000107625.82057.221802429.22670-0.239802.490601.44420K562CPEB4
DDX50ENSG00000107625.81388.895201808.67780-0.381005.341003.61620K562DDX21
DDX50ENSG00000107625.81384.227201726.01430-0.318402.874801.81330K562DDX3X
DDX50ENSG00000107625.83887.866902513.539900.6292016.3353014.87370K562DDX47
DDX50ENSG00000107625.82999.345802558.141800.229502.662801.80760K562DKC1
DDX50ENSG00000107625.81189.857201557.84560-0.388802.923301.98560K562EFTUD2
DDX50ENSG00000107625.81592.039001895.91280-0.252002.800101.58230K562EIF2S1
DDX50ENSG00000107625.82756.491203533.58090-0.358306.061104.68740K562EIF4G2
DDX50ENSG00000107625.81213.777901574.08090-0.375004.292702.63360K562EWSR1
DDX50ENSG00000107625.8970.846101396.80890-0.524802.902502.02640K562FMR1
DDX50ENSG00000107625.81567.464501875.32920-0.258702.712602.11260K562FXR1
DDX50ENSG00000107625.81515.046001838.73470-0.279402.924101.75170K562GLRX3
DDX50ENSG00000107625.82884.539402253.800100.355902.853501.59960K562HNRNPK
DDX50ENSG00000107625.83385.571702246.023100.591908.260406.89920K562HSPD1
DDX50ENSG00000107625.8813.287501119.18710-0.460602.714201.79180K562KRR1
DDX50ENSG00000107625.82943.410303766.37790-0.355703.493102.22440K562MSI2
DDX50ENSG00000107625.81483.080501933.18410-0.382406.158104.99220K562MTPAP
DDX50ENSG00000107625.81639.075602147.97330-0.390108.900907.66520K562NSUN2
DDX50ENSG00000107625.83326.230604017.92510-0.272603.898202.75570K562PUM2
DDX50ENSG00000107625.81711.352702209.42500-0.368503.411202.47290K562RBM22
DDX50ENSG00000107625.82265.028001544.327000.552505.434704.28630K562RPS19
DDX50ENSG00000107625.81578.686802119.45360-0.425006.599605.38770K562SF3A3
DDX50ENSG00000107625.8896.447001246.49170-0.475602.735701.76720K562SF3B1
DDX50ENSG00000107625.81129.580001897.53560-0.7483011.833709.98610K562SF3B4
DDX50ENSG00000107625.8702.40590965.92300-0.459603.724502.48480K562SRFBP1
DDX50ENSG00000107625.81893.245102171.42170-0.197802.425201.54190K562SRPK2
DDX50ENSG00000107625.81488.028502286.08460-0.619507.742306.48700K562TARDBP
DDX50ENSG00000107625.8966.314401338.05400-0.469603.573801.65220K562TFIP11
DDX50ENSG00000107625.8818.761101074.07660-0.391602.669701.75240K562TIAL1
DDX50ENSG00000107625.8889.620101141.17040-0.359302.844501.88250K562TRIM56
DDX50ENSG00000107625.82453.780901769.077000.471904.072702.69130K562U2AF1
DDX50ENSG00000107625.82510.274001826.703200.458506.701605.45930K562U2AF2
DDX50ENSG00000107625.81051.957201553.61190-0.562605.476904.00020K562UCHL5
DDX50ENSG00000107625.81635.140701948.82240-0.253202.075101.31880K562WDR3
DDX50ENSG00000107625.83406.698604112.36080-0.271602.626301.37470K562WRN
DDX50ENSG00000107625.83103.387003910.37800-0.333503.350202.38910K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for DDX50 is not available on ENCORE database.





20 RBPs can affect the splicing of DDX50 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEDDX50ENSG00000107625.8chr10+706659817066609370661072706612277066646670666763-0.056000.000200.00562excludedK562SRSF1
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.144000.000330.00809includedHepG2U2AF1
SEDDX50ENSG00000107625.8chr10+706939547069411670679587706797377069455470694674-0.093000.000000.00005excludedK562AKAP8L
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.230000.000000.00000includedK562XRCC6
SEDDX50ENSG00000107625.8chr10+707008157070095070696691706968517070610770706603-0.062000.000030.00064excludedK562AQR
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.145000.000000.00001includedK562FXR1
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.057000.000490.06879includedK562DROSHA
SEDDX50ENSG00000107625.8chr10+706939547069411670679587706797377069455470694674-0.063000.000100.00251excludedK562PUS1
SEDDX50ENSG00000107625.8chr10+706659817066609370661072706612277066646670666763-0.055000.000120.00282excludedK562PUS1
SEDDX50ENSG00000107625.8chr10+7066766670667726706664667066676370670062706701380.060000.000140.07949includedK562SSB
SEDDX50ENSG00000107625.8chr10+706939547069411670679587706797377069455470694674-0.206000.000000.00000excludedK562U2AF1
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.136000.000620.04658includedHepG2HNRNPL
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.099000.001700.09313includedK562HNRNPL
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.146000.000300.07388includedHepG2NOL12
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.106000.000230.04336includedK562UPF1
SEDDX50ENSG00000107625.8chr10+706939547069411670679587706797377069455470694674-0.090000.000200.00509excludedK562U2AF2
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.090000.000430.01957includedK562TARDBP
SEDDX50ENSG00000107625.8chr10+706939547069411670679587706797377069455470694674-0.098000.000000.00047excludedK562TARDBP
SEDDX50ENSG00000107625.8chr10+706939547069411670679587706797377069455470694674-0.071000.000010.00067excludedK562HNRNPU
SEDDX50ENSG00000107625.8chr10+706659817066609370661072706612277066646670666763-0.071000.000000.00030excludedK562AGGF1
SEDDX50ENSG00000107625.8chr10+706957617069583570694554706946747069669170696851-0.076000.002660.05814excludedK562AGGF1
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.182000.000000.00123includedK562EIF3G
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.137000.000000.00013includedK562PTBP1
SEDDX50ENSG00000107625.8chr10+7066598170666093706610727066122770666466706667630.170000.000270.00637includedK562HNRNPC
MXEDDX50ENSG00000107625.8chr10+70695761706958357070081570700950706945547069467470702968707030130.160000.000200.005641includedK562PUS1
MXEDDX50ENSG00000107625.8chr10+70696691706968517070296870703013706945547069467470706107707066030.109000.000030.000441includedK562AQR
MXEDDX50ENSG00000107625.8chr10+70696691706968517070296870703013706795877067973770706107707066030.064000.000110.001331includedK562AQR
MXEDDX50ENSG00000107625.8chr10+70696691706968517070296870703013706945547069467470706107707066030.112000.000570.012681includedK562SF3B4

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for DDX50 is not avaliable on ENCORE database.