DDX50      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-35BGHcLV49-36
idx00
TRCN#_or_BGC#BGC#0000901BGC#0000901
shRNA_or_gRNA_sequenceGCTCCATAATGTCCCCCCAGGCTCCATAATGTCCCCCCAG
PAMaggagg
NameDDX50_74DDX50_74
Sample_IDBGHcLV49-35BGHcLV49-36
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameDDX50DDX50
qPCR_result0.00.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc50165827
WB_result50.549.6
Ave_WB
WB_DONE_date6/19/25,6/27/25
MW83KD
IP
antibody_Cat#A301-312Alot # 1
Antibody DCC IDENCAB756NJZ
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99179918




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX50Product_ID: A301-312A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX50-human		 DDX50-hepg2-CRISPR-A301-312A-LICOR.png<br>Caption: Western blot following CRISPR against DDX50 in HepG2 whole cell lysate using DDX50 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX50. DDX50 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 DDX50-K562-CRISPR-A301-312A.png<br>Caption: Western blot following CRISPR against DDX50 in K562 whole cell lysate using DDX50 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX50. DDX50 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-35BGHcLV49-36
Sample_IDBGHcLV49-35BGHcLV49-36
Sample Name
Sample NameDDX50-BGHcLV49-35DDX50-BGHcLV49-36
RBPDDX50DDX50
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1434214343




Library-Prep Information
BGHcLV49-35BGHcLV49-36
Sample #3940
Sample NameDDX50-BGHcLV49-35DDX50-BGHcLV49-36
Sample_Name_Alias
Index Well PositionA06B06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp261273
Peak_Molarity78.2065.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-35BGHcLV49-36
RBPDDX50DDX50
Batch_IDBGHcLV49BGHcLV49
WB_result50.50049.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47844785




Sequencing Information
BGHcLV49-35BGHcLV49-36
Sample_IDBGHcLV49-35BGHcLV49-36
Sample NameDDX50-BGHcLV49-35DDX50-BGHcLV49-36
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35113512




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database