DDX50      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-35BGHcLV49-36
idx00
TRCN#_or_BGC#BGC#0000901BGC#0000901
shRNA_or_gRNA_sequenceGCTCCATAATGTCCCCCCAGGCTCCATAATGTCCCCCCAG
PAMaggagg
NameDDX50_74DDX50_74
Sample_IDBGHcLV49-35BGHcLV49-36
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameDDX50DDX50
qPCR_result0.00.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc50165827
WB_result50.549.6
Ave_WB
WB_DONE_date6/19/25,6/27/25
MW83KD
IP
antibody_Cat#A301-312Alot # 1
Antibody DCC IDENCAB756NJZ
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99159916




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX50Product_ID: A301-312A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX50-human		 DDX50-hepg2-CRISPR-A301-312A-LICOR.png<br>Caption: Western blot following CRISPR against DDX50 in HepG2 whole cell lysate using DDX50 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX50. DDX50 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 DDX50-K562-CRISPR-A301-312A.png<br>Caption: Western blot following CRISPR against DDX50 in K562 whole cell lysate using DDX50 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX50. DDX50 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-35BGHcLV49-36
Sample_IDBGHcLV49-35BGHcLV49-36
Sample Name
Sample Name
RBPDDX50DDX50
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1434214343




Library-Prep Information
BGHcLV49-35BGHcLV49-36
Sample #3940
Sample NameDDX50-BGHcLV49-35DDX50-BGHcLV49-36
Sample_Name_Alias
Index Well PositionA06B06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp
Peak_Molarity
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_method
SampleQC_date
Sample_IDBGHcLV49-35BGHcLV49-36
RBPDDX50DDX50
Batch_IDBGHcLV49BGHcLV49
WB_result50.50049.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusIndexAssignedIndexAssigned
ProjectENCORE2ENCORE2
ID47844785




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database