DDX21

General Information

Gene Name : DDX21
Gene ID : ENSG00000165732.12
Essential Gene : Yes

Functions

rRNA processing
Viral RNA regulation
snoRNA / snRNA / telomerase
Other

Localization(RBPImage: DDX21)

Nuclei
Nucleolus
Nuclear release mitosis

Domains (AlphaFold: DDX21)

Helicase

Antibody Validation

MBLI: RN090PW

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	DDX21	ENSG00000165732.12	140.57	123.69	132.13	ENCSR000AEF
HepG2	DDX21	ENSG00000165732.12	57.73	107.26	82.50	ENCSR985KAT
K562	DDX21	ENSG00000165732.12	228.73	245.25	236.99	ENCSR000AEO

Assays

5 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 284	ENCSR040QLV	DDX21	K562	Released	ENCAB315FWL	ENCSR266XXD
knockdown followed by RNA-seq	DDX21-BGKLV11-K562	ENCSR961WVL	DDX21	K562	Released		ENCSR129RWD	shRNA	KD% = 90.9,86.2
knockdown followed by RNA-seq	DDX21-BGHLV14-HepG2	ENCSR485ZTC	DDX21	HepG2	Released		ENCSR003EKR	shRNA	KD% = 90.6,92.5
knockdown followed by RNA-seq	DDX21-BGKcLV02-K562	ENCSR384LKC	DDX21	K562	Released		ENCSR163JUC	CRISPR	KD% = 94.5,96.4
knockdown followed by RNA-seq	DDX21-BGHcLV03-HepG2	ENCSR231RKU	DDX21	HepG2	Released		ENCSR105NML	CRISPR	KD% = NO band, need add more protein,

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
DDX21	284	K562	ENCSR040QLV	ENCSR266XXD	ENCAB315FWL	RN090PW	1	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
DDX21	ENCAB315FWL	RN090PW	1		Released, ENCODE3	Released, ENCODE3	BGHLV14-9, Released, ENCODE3	BGKLV11-9, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
DDX21	K562	shRNA	ENCSR961WVL	ENCSR129RWD	DDX21-LV11-9,DDX21-LV11-10	Nontarget-LV11-1,nontarget-LV11-2	90.9,86.2	Released	ENCODE3
DDX21	HepG2	shRNA			BGHLV12-11,BGHLV12-12	BGHLV12-1,BGHLV12-2		NotSatisfied	ENCODE3
DDX21	HepG2	shRNA	ENCSR485ZTC	ENCSR003EKR	DDX21_BGHLV14-9,DDX21_BGHLV14-10	NT_BGHLV14-1_1,NT_BGHLV14-2_1	90.6,92.5	Released	ENCODE3
DDX21	K562	CRISPR	ENCSR384LKC	ENCSR163JUC	DDX21_BGKcLV02-11,DDX21_BGKcLV02-12	NT_BGKcLV02-1,NT_BGKcLV02-2	94.5,96.4	Released	ENCODE3
DDX21	HepG2	CRISPR	ENCSR231RKU	ENCSR105NML	DDX21_BGHcLV03-9,DDX21_BGHcLV03-10	NT_BGHcLV03-1,NT_BGHcLV03-2	NO band, need add more protein,	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

DDX21: Click to RBP Image Database

Nuclei

Nucleolus

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
DDX21	Product_ID: RN090PW Lot_ID: 1 Source: MBLI Target Name: DDX21-human

RNA Targets

DDX21 protein binds to 75 RNA regions.

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Proteins bound to RNA

DDX21 RNA is bound by 35 proteins.

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DDX21 protein has 75 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
DDX21	chr20	26189971	26190023	-	4.80920	400.00000	K562	MIR663A	intron-Distal,intron-Proximal
DDX21	chr9	136217720	136217754	+	3.32413	400.00000	K562	RPL7A	intron-Distal
DDX21	chr11	122930079	122930123	-	3.23813	400.00000	K562	HSPA8	5'-UTR,intron-Distal,intron-Proximal
DDX21	chr11	122929644	122929665	-	3.35204	400.00000	K562	HSPA8	5'-UTR,intron-Distal
DDX21	chr11	122930040	122930079	-	3.20382	400.00000	K562	HSPA8	5'-UTR,intron-Distal,intron-Proximal
DDX21	chr11	17097323	17097363	-	3.39096	87.51971	K562	RPS13	intron-Distal
DDX21	chr6	16266414	16266440	+	4.64911	83.98397	K562	GMPR	intron-Distal
DDX21	chr9	136217754	136217770	+	3.40397	76.17843	K562	RPL7A	intron-Distal,intron-Proximal
DDX21	chr6	16266440	16266483	+	4.12990	72.20744	K562	GMPR	intron-Distal
DDX21	chr11	17096255	17096275	-	4.01472	70.58401	K562	RPS13	intron-Distal

Showing 1 to 10 of 75 entries

35 proteins are bound to DDX21 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr10	70744725	70744750	+	3'-UTR	6.61874	400.00000	K562	PABPC4
chr10	70744704	70744725	+	3'-UTR	6.35227	400.00000	K562	PABPC4
chr10	70715925	70715992	+	5'-UTR,CDS	4.45830	43.35622	K562	DDX3X
chr10	70715992	70716069	+	CDS,intron-Proximal	3.95860	41.57971	K562	DDX3X
chr10	70744704	70744769	+	3'-UTR	3.80034	36.51660	K562	PUM2
chr10	70743286	70743367	+	3'-UTR	3.07471	34.08669	K562	PUM2
chr10	70719875	70719995	+	CDS	5.66641	32.43569	HepG2	BCLAF1
chr10	70743673	70743759	+	3'-UTR	7.30206	31.06342	HepG2	SUB1
chr10	70719924	70719999	+	CDS	3.25865	29.95597	K562	UCHL5
chr10	70719878	70719987	+	CDS	3.14017	29.65795	K562	ZNF622
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 136 entries

No autoregularoty interactions between protein DDX21 and its RNA.

Diffrential Expression

8,193 differentially expressed genes after Knockdown/Knockout DDX21 protein.

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154 RBPs can affect the levels of DDX21.

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8,193 differentially expressed genes after Knockdown/Knockout DDX21 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
TFPI	ENSG00000003436.10	1978.03010	7531.56590	-1.92890	88.64940	85.82460	HepG2	DDX21
JHDM1D	ENSG00000006459.6	375.71400	1708.50570	-2.18500	73.87920	71.24280	HepG2	DDX21
TMEM98	ENSG00000006042.7	407.72520	1344.76480	-1.72170	44.76370	42.52520	HepG2	DDX21
SLC7A2	ENSG00000003989.12	2350.68360	5554.48240	-1.24060	37.82790	35.76870	HepG2	DDX21
HIVEP2	ENSG00000010818.4	382.42860	1055.21210	-1.46430	30.30750	28.42300	HepG2	DDX21
IL32	ENSG00000008517.12	3751.30720	1716.25490	1.12800	29.32680	27.46990	HepG2	DDX21
RHOBTB2	ENSG00000008853.12	722.64230	258.60090	1.48250	24.45760	22.72530	HepG2	DDX21
CROT	ENSG00000005469.7	587.37940	195.59110	1.58640	23.94220	22.22660	HepG2	DDX21
LAP3	ENSG00000002549.8	1093.28450	2013.75150	-0.88120	16.25960	14.77890	HepG2	DDX21
ABCB4	ENSG00000005471.11	338.22630	736.82180	-1.12330	16.06030	14.58470	HepG2	DDX21
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

154 RBPs can affect the gene expression of DDX21 :

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Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
DDX21	ENSG00000165732.8	1012.37700	3628.94450	-1.84180	164.49750	160.82930	HepG2	DNAJC2
DDX21	ENSG00000165732.8	2546.01710	7054.97990	-1.47040	119.85760	117.84500	HepG2	SSB
DDX21	ENSG00000165732.8	3139.60830	16652.52350	-2.40710	116.85080	112.74680	K562	DDX21
DDX21	ENSG00000165732.8	1239.83880	3968.77340	-1.67850	79.84900	77.85850	HepG2	TIAL1
DDX21	ENSG00000165732.8	2376.04290	4921.51100	-1.05050	74.43550	71.74420	HepG2	HNRNPL
DDX21	ENSG00000165732.8	2038.64710	4311.81500	-1.08070	52.26530	50.30060	HepG2	PUS1
DDX21	ENSG00000165732.8	624.13870	3240.05010	-2.37610	45.20690	42.96190	HepG2	DDX21
DDX21	ENSG00000165732.8	2166.44380	4050.09110	-0.90260	37.75850	36.01280	HepG2	DKC1
DDX21	ENSG00000165732.8	9225.69130	18104.85470	-0.97260	33.03420	30.36340	K562	CPSF6
DDX21	ENSG00000165732.8	1633.45890	4106.27300	-1.32990	30.92760	28.67800	HepG2	XRN2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 188 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

92 alternative splicing events after Knockdown/Knockout DDX21 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SNRNP70	ENSG00000104852.10	chr19	+	49605370	49606844	49604646	49604728	49607890	49607992	0.06600	0.00006	included	K562	DDX21
SE	BAG6	ENSG00000204463.8	chr6	-	31607276	31607423	31606804	31607003	31607975	31608083	0.14400	0.00888	included	K562	DDX21
SE	HNRNPDL	ENSG00000152795.13	chr4	-	83346715	83346820	83345781	83346036	83347189	83347303	0.17200	0.00000	included	K562	DDX21
SE	MFF	ENSG00000168958.15	chr2	+	228217229	228217289	228211440	228212100	228220392	228220477	0.31500	0.00080	included	K562	DDX21
SE	PABPC4	ENSG00000090621.9	chr1	-	40029507	40029594	40029285	40029413	40030094	40030214	0.16100	0.00241	included	K562	DDX21
SE	SRRM1	ENSG00000133226.12	chr1	+	24973569	24973699	24973157	24973280	24975349	24975520	0.19800	0.00018	included	K562	DDX21
SE	RPS24	ENSG00000138326.14	chr10	+	79799961	79799983	79796951	79797062	79800372	79800455	0.11800	0.00001	included	K562	DDX21
SE	AC079466.1	ENSG00000266976.1	chr19	+	29101888	29103061	29097594	29098011	29103345	29106136	-0.10400	0.00646	excluded	K562	DDX21
SE	EIF4A2	ENSG00000156976.10	chr3	+	186506098	186506209	186505591	186505671	186506913	186507080	-0.06900	0.00003	excluded	K562	DDX21
SE	CSNK1D	ENSG00000141551.10	chr17	-	80203851	80203915	80202269	80202707	80206750	80207372	-0.20900	0.00336	excluded	K562	DDX21

Showing 1 to 10 of 92 entries

12 RBPs can affect the splicing of DDX21 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	DDX21	ENSG00000165732.8	chr10	+	70731646	70731808			70729956	70730106	70733300	70733420	-0.14000	0.00000	excluded	K562	AKAP8L
SE	DDX21	ENSG00000165732.8	chr10	+	70734356	70734499			70733300	70733420	70737284	70737485	-0.05900	0.00001	excluded	K562	AQR
SE	DDX21	ENSG00000165732.8	chr10	+	70741218	70741337			70738621	70738732	70742298	70744829	-0.05800	0.00000	excluded	K562	AQR
SE	DDX21	ENSG00000165732.8	chr10	+	70721798	70721913			70719561	70720005	70723046	70723225	-0.06000	0.00000	excluded	K562	AQR
SE	DDX21	ENSG00000165732.8	chr10	+	70731646	70731808			70729956	70730106	70733300	70733420	-0.23900	0.00000	excluded	K562	PUS1
SE	DDX21	ENSG00000165732.8	chr10	+	70731646	70731808			70729956	70730106	70733300	70733420	-0.12900	0.00000	excluded	K562	UTP18
SE	DDX21	ENSG00000165732.8	chr10	+	70731646	70731808			70729956	70730106	70733300	70733420	-0.14000	0.00000	excluded	K562	RBM15
SE	DDX21	ENSG00000165732.8	chr10	+	70731646	70731808			70729956	70730106	70733300	70733420	-0.16200	0.00000	excluded	K562	AGGF1
SE	DDX21	ENSG00000165732.8	chr10	+	70731646	70731808			70729956	70730106	70733300	70733420	-0.08200	0.00011	excluded	K562	PABPC4
MXE	DDX21	ENSG00000165732.8	chr10	+	70733300	70733420	70738597	70738732	70729956	70730106	70741292	70741337	0.12100	0.00000	1included	K562	AGGF1

Showing 1 to 10 of 20 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

DDX21

General Information

Functions

Localization(RBPImage: DDX21)

Domains (AlphaFold: DDX21)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

DDX21 protein has 75 RNA targets:

35 proteins are bound to DDX21 RNA:

No autoregularoty interactions between protein DDX21 and its RNA.

Diffrential Expression

8,193 differentially expressed genes after Knockdown/Knockout DDX21 protein :

154 RBPs can affect the gene expression of DDX21 :

92 alternative splicing events after Knockdown/Knockout DDX21 protein :

12 RBPs can affect the splicing of DDX21 :

No autoregularoty alternative splicing events between protein DDX21 and its RNA.