SPAG9

General Information

Gene Name : SPAG9
Gene ID : ENSG00000008294.20
Essential Gene : No

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: SPAG9)

Not Available

Antibody Validation

View validation images for all SPAG9 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	SPAG9	ENSG00000008294.20	26.12	23.54	24.83	ENCSR000AEF
HepG2	SPAG9	ENSG00000008294.20	34.27	44.91	39.59	ENCSR985KAT
K562	SPAG9	ENSG00000008294.20	43.17	39.16	41.16	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	SPAG9-BGKcLV33-K562	ENCSR642LZB	SPAG9	K562	Released		ENCSR271ZAB	CRISPR	KD% = no band on the position,
knockdown followed by RNA-seq	SPAG9-BGHcLV31-HepG2	ENCSR293VAR	SPAG9	HepG2	Released		ENCSR452TFV	CRISPR	KD% = 83.7,72.2

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
SPAG9	ENCAB002WNS	A300-336A	4				BGHcLV31-63, Released, ENCODE4	BGKcLV33-67, Released, ENCODE4
SPAG9	ENCAB167KJP	A300-337A	2				BGHcLV31-63, Released, ENCODE4	BGKcLV33-67, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
SPAG9	K562	CRISPR			BGKcLV33-63,BGKcLV33-64	BGKcLV33-1,BGKcLV33-2	89.5,77.1	NotSatisfied	ENCODE4
SPAG9	K562	CRISPR	ENCSR642LZB	ENCSR271ZAB	SPAG9-BGKcLV33-67,SPAG9-BGKcLV33-68	NT-BGKcLV33-1,NT-BGKcLV33-2	no band on the position,	Released	ENCODE4
SPAG9	HepG2	CRISPR	ENCSR293VAR	ENCSR452TFV	SPAG9-BGHcLV31-63,SPAG9-BGHcLV31-64	NT-BGHcLV31-1,NT-BGHcLV31-2	83.7,72.2	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SPAG9	Product_ID: A300-336A Lot_ID: 4 Source: Bethyl Labs Target Name: SPAG9-human
SPAG9	Product_ID: A300-337A Lot_ID: 2 Source: Bethyl Labs Target Name: SPAG9-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for SPAG9 is not avaliable on ENCORE database.

Proteins bound to RNA

SPAG9 RNA is bound by 47 proteins.

CanvasJS.com

eCLIP assay for SPAG9 is not avaliable on ENCORE database.

47 proteins are bound to SPAG9 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr17	49097526	49097604	-	CDS,intron-Distal	4.53759	36.25314	HepG2	BCLAF1
chr17	49097541	49097622	-	CDS,intron-Distal,intron-Proximal	3.73227	35.35233	K562	ZNF622
chr17	49097518	49097588	-	CDS,intron-Distal,intron-Proximal	3.61546	32.62672	K562	UCHL5
chr17	49067108	49067233	-	CDS,intron-Distal,intron-Proximal	3.69034	30.31382	HepG2	BCLAF1
chr17	49197515	49197724	-	CDS,intron-Distal	3.59430	28.48552	HepG2	EFTUD2
chr17	49083486	49083553	-	CDS,intron-Distal	5.97662	27.62668	HepG2	BCLAF1
chr17	49118898	49118993	-	CDS,intron-Distal	3.78911	25.64373	K562	ZNF622
chr17	49058783	49058881	-	intron-Distal	6.85994	25.63430	HepG2	PTBP1
chr17	49118922	49118999	-	CDS,intron-Distal	4.10807	25.53379	HepG2	BCLAF1
chr17	49053209	49053317	-	CDS,intron-Distal,intron-Proximal	4.97235	25.39538	HepG2	U2AF2
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 260 entries

eCLIP assay for SPAG9 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for SPAG9 is not available on ENCORE database.
118 RBPs can affect the levels of SPAG9.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for SPAG9 is not available on ENCORE database.

118 RBPs can affect the gene expression of SPAG9 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SPAG9	ENSG00000008294.16	2302.48730	4819.62800	-1.06570	50.85830	48.87500	HepG2	PCBP1
SPAG9	ENSG00000008294.16	1618.69710	3366.76020	-1.05650	45.78440	43.75490	HepG2	SMN1
SPAG9	ENSG00000008294.16	2212.63880	4326.30380	-0.96740	43.73860	41.88740	HepG2	DKC1
SPAG9	ENSG00000008294.16	2547.75470	4605.92060	-0.85430	41.77250	39.94430	HepG2	PUS1
SPAG9	ENSG00000008294.16	2833.96930	5093.68810	-0.84590	38.95590	37.55340	HepG2	SSB
SPAG9	ENSG00000008294.16	1924.27760	5382.34600	-1.48390	36.31310	34.23060	HepG2	NCBP2
SPAG9	ENSG00000008294.16	1878.83980	3311.33970	-0.81760	33.35100	31.18540	HepG2	ILF2
SPAG9	ENSG00000008294.16	1260.84450	2774.27080	-1.13770	32.34930	29.85590	HepG2	HNRNPM
SPAG9	ENSG00000008294.16	1701.54130	2880.12480	-0.75930	31.47130	29.50200	HepG2	DNAJC2
SPAG9	ENSG00000008294.16	1462.39560	3020.77050	-1.04660	29.22010	26.21780	HepG2	EIF2S2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 141 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for SPAG9 is not available on ENCORE database.

28 RBPs can affect the splicing of SPAG9 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SPAG9	ENSG00000008294.16	chr17	-	49053223	49053262	49052131	49052308	49054468	49054582	0.19800	0.00000	included	HepG2	G3BP1
SE	SPAG9	ENSG00000008294.16	chr17	-	49109041	49109083	49098518	49098726	49118861	49119012	0.32500	0.00021	included	K562	SRSF1
SE	SPAG9	ENSG00000008294.16	chr17	-	49145292	49145373	49133772	49133843	49156944	49157065	0.09800	0.00131	included	HepG2	HNRNPM
SE	SPAG9	ENSG00000008294.16	chr17	-	49053223	49053262	49052131	49052308	49054468	49054582	0.48200	0.00000	included	HepG2	HNRNPK
SE	SPAG9	ENSG00000008294.16	chr17	-	49053223	49053262	49052131	49052308	49054468	49054582	-0.19000	0.00012	excluded	HepG2	U2AF2
SE	SPAG9	ENSG00000008294.16	chr17	-	49091592	49091714	49084629	49084687	49097519	49097619	-0.12700	0.00002	excluded	K562	AQR
SE	SPAG9	ENSG00000008294.16	chr17	-	49059884	49060033	49057106	49057278	49064472	49064627	-0.21900	0.00000	excluded	K562	AQR
SE	SPAG9	ENSG00000008294.16	chr17	-	49053223	49053262	49052131	49052308	49054468	49054582	-0.22300	0.00046	excluded	HepG2	FTO
SE	SPAG9	ENSG00000008294.16	chr17	-	49053223	49053262	49052131	49052308	49054468	49054582	0.22200	0.00000	included	HepG2	SRSF1
SE	SPAG9	ENSG00000008294.16	chr17	-	49109041	49109083	49098518	49098726	49118861	49119012	0.31700	0.00000	included	HepG2	SRSF1

Showing 1 to 10 of 55 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

SPAG9

General Information

Functions

Localization

Domains (AlphaFold: SPAG9)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for SPAG9 is not avaliable on ENCORE database.

47 proteins are bound to SPAG9 RNA:

eCLIP assay for SPAG9 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for SPAG9 is not available on ENCORE database.

118 RBPs can affect the gene expression of SPAG9 :

The Knockdown/knockout RNA-Seq assay for SPAG9 is not available on ENCORE database.

28 RBPs can affect the splicing of SPAG9 :

The Knockdown/knockout RNA-Seq assay for SPAG9 is not avaliable on ENCORE database.