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SPAG9
CRISPR
in K562
Control:
NT-BGKcLV33
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
SPAG9
Cell_Line
K562
Method
CRISPR
Exp_Name
SPAG9-BGKcLV33-K562
ENCODE_series_ID
ENCSR495FVC
Batch_ID
BGKcLV33
Pool ID
Pool-220418
Local_Set_Name
set53
ENCODE_KD_Exp_ID
ENCSR642LZB
ENCODE_CN_Exp_ID
ENCSR271ZAB
Rep1
SPAG9-BGKcLV33-67
Rep2
SPAG9-BGKcLV33-68
CN1
NT-BGKcLV33-1
CN2
NT-BGKcLV33-2
Rep1_qPCR
Rep2_qPCR
Rep1_WB
no band on the position
Rep2_WB
Antibody Cat#
A305-118A
Antibody Lot#
LOT #1
Antibody DCC ID
Status
Submitted
Project
ENCODE4
ID
914
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
NotSatisfied
)
BGKcLV33-63
BGKcLV33-63
BGKcLV33-64
BGKcLV33-64
idx
1165
1256
1166
1257
TRCN#_or_BGC#
BGC#0000881
BGC#0000877
BGC#0000881
BGC#0000877
shRNA_or_gRNA_sequence
TATCGGGCGCTATGACGAGG
GCAGTTCCAGTAACCTCCGG
TATCGGGCGCTATGACGAGG
GCAGTTCCAGTAACCTCCGG
PAM
AGG
CGG
AGG
CGG
Name
SPAG9_98
SNUPN_92
SPAG9_98
SNUPN_92
Sample_ID
BGKcLV33-63
BGKcLV33-63
BGKcLV33-64
BGKcLV33-64
transduction_Date
12/7/21
2/22/22
12/7/21
2/22/22
days
D6
D6
D6
D6
RBP_name
SPAG9
SNUPN
SPAG9
SNUPN
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc
434
2213
417
1989
WB_result
89.5
0.0
77.1
0.0
Ave_WB
83.3
0.0
WB_DONE_date
2/1/22
3/10/22
MW
146kd
41kd
IP
antibody_Cat#
A300-336A
A305-567A
LOT #4
LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM
0
0
0
0
Rep2_TPM
0
0
0
0
Action
Library_start_date
repeat_library
Note
ID
5305
5398
5306
5399
Western Blot
Western Blot info was not avaliable
Experiment Status
BGKcLV33-63
BGKcLV33-63
BGKcLV33-64
BGKcLV33-64
Sample_ID
BGKcLV33-63
BGKcLV33-63
BGKcLV33-64
BGKcLV33-64
Sample Name
Sample Name
RBP
SPAG9
SNUPN
SPAG9
SNUPN
Cell_Line
K562
K562
K562
K562
Exp UID
Status
NotSatisfied
NotSatisfied
NotSatisfied
NotSatisfied
Status_date
2022-03-30
2022-03-30
2022-03-30
2022-03-30
Project
ENCODE4
ENCODE4
ENCODE4
ENCODE4
Note
ID
10749
10787
10750
10788
Library-Prep
Sequencing
Library-Prep Information
Library info was not avaliable
Sequencing Information
Sequencing info was not avaliable
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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