BCLAF1

General Information

Gene Name : BCLAF1
Gene ID : ENSG00000029363.16
Essential Gene : No

Functions

RNA stability & decay

Localization(RBPImage: BCLAF1)

Nuclei
Mutually Exclusive from Nucleolus
Speckles
Variable Nuclear Intensity
Nuclear release mitosis

Domains (AlphaFold: BCLAF1)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A300-608A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	BCLAF1	ENSG00000029363.16	149.36	152.01	150.69	ENCSR000AEF
HepG2	BCLAF1	ENSG00000029363.16	89.10	133.35	111.22	ENCSR985KAT
K562	BCLAF1	ENSG00000029363.16	119.51	130.91	125.21	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 643	ENCSR876EYA	BCLAF1	HepG2	Released	ENCAB012FUX	ENCSR128BUO
knockdown followed by RNA-seq	BCLAF1-BGKLV19-K562	ENCSR410ZPU	BCLAF1	K562	Released		ENCSR245BNJ	shRNA	KD% = 44.8,37.5
knockdown followed by RNA-seq	BCLAF1-BGHLV17-HepG2	ENCSR481AYC	BCLAF1	HepG2	Released		ENCSR856ZRV	shRNA	KD% = 99.4,98.3

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
BCLAF1	643	HepG2	ENCSR876EYA	ENCSR128BUO	ENCAB012FUX	A300-608A	1	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
BCLAF1	ENCAB012FUX	A300-608A	1		Released, ENCODE3		BGHLV17-5, Released, ENCODE3	BGKLV19-7, Released, ENCODE3
BCLAF1		A300-608A	2		Released, ENCODE3		BGHLV17-5, Released, ENCODE3	BGKcLV40-3, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
BCLAF1	K562	shRNA	ENCSR410ZPU	ENCSR245BNJ	BCLAF1_BGKLV19_7A,BCLAF1_BGKLV19_8A	NT_BGKLV19_1A,NT_BGKLV19_2A	44.8,37.5	Released	ENCODE3
BCLAF1	HepG2	shRNA			BGHLV16-7,BGHLV16-8	BGHLV16-1,BGHLV16-2		NotSatisfied	ENCODE3
BCLAF1	HepG2	shRNA	ENCSR481AYC	ENCSR856ZRV	BCLAF1_BGHLV17_5,BCLAF1_BGHLV17_6	NT_BGHLV17_63,NT_BGHLV17_64	99.4,98.3	Released	ENCODE3
BCLAF1	K562	CRISPR			BGKcLV37-3,BGKcLV37-4	BGKcLV37-1,BGKcLV37-2	0.0,0.0	NotSatisfied	ENCORE2
BCLAF1	K562	CRISPR			BCLAF1-BGKcLV40-3,BCLAF1-BGKcLV40-4	NT-BGKcLV40-1,NT-BGKcLV40-2	80.9,92.2005	Submitting	ENCORE2
BCLAF1	HepG2	CRISPR			BGHcLV36-3,BGHcLV36-4	BGHcLV36-1,BGHcLV36-2	NO CLEAR BAND ON THE POSITION,	NotSatisfied	ENCORE2
BCLAF1	HepG2	CRISPR			BCLAF1-BGHcLV41-3,BCLAF1-BGHcLV41-4	NT-BGHcLV41-1,NT-BGHcLV41-2	need discussion ,	Submitting	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

BCLAF1: Click to RBP Image Database

Nuclei

Mutually Exclusive from Nucleolus

Speckles

Variable Nuclear Intensity

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
BCLAF1	Product_ID: A300-608A Lot_ID: 1 Source: Bethyl Labs Target Name: BCLAF1-human

RNA Targets

BCLAF1 protein binds to 32,570 RNA regions.

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Proteins bound to RNA

BCLAF1 RNA is bound by 33 proteins.

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BCLAF1 protein has 32,570 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
BCLAF1	chr14	39648438	39648446	+	5.09559	81.36782	HepG2	PNN	3'-UTR,CDS
BCLAF1	chr10	7749166	7749178	+	3.65400	61.15303	HepG2	ITIH2	CDS,intron-Distal
BCLAF1	chrX	108921495	108921507	-	4.34542	58.04538	HepG2	ACSL4	CDS,intron-Distal
BCLAF1	chr2	44201291	44201308	-	3.74806	55.04008	HepG2	LRPPRC	CDS,intron-Distal
BCLAF1	chr7	26237075	26237082	-	3.75086	51.51893	HepG2	HNRNPA2B1	CDS,intron-Distal,intron-Proximal
BCLAF1	chr4	155529693	155529701	-	3.98118	51.27917	HepG2	FGG	CDS,intron-Distal,intron-Proximal
BCLAF1	chr14	39649728	39649754	+	4.80843	48.82062	HepG2	PNN	CDS
BCLAF1	chr2	242202162	242202280	-	3.31238	48.64917	HepG2	HDLBP	CDS,intron-Distal,intron-Proximal
BCLAF1	chr1	146687497	146687513	-	3.58020	45.31320	HepG2	FMO5	CDS,intron-Distal,intron-Proximal
BCLAF1	chrX	108904835	108904874	-	3.73382	37.56668	HepG2	ACSL4	CDS,intron-Distal

Showing 1 to 10 of 100 entries

33 proteins are bound to BCLAF1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr6	136590614	136590741	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.24487	44.57980	K562	ZNF622
chr6	136579724	136579850	-	3'-UTR,intron-Distal,intron-Proximal	3.93722	42.91496	K562	KHSRP
chr6	136599655	136599815	-	CDS,intron-Distal	3.71513	39.86227	K562	UCHL5
chr6	136599689	136599815	-	CDS,intron-Distal	3.02995	29.49397	K562	ZNF622
chr6	136596971	136597069	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.39232	28.29451	K562	ZNF622
chr6	136598914	136599004	-	CDS,intron-Distal,intron-Proximal	5.51979	23.89296	K562	AQR
chr6	136599010	136599096	-	CDS,intron-Distal	3.19133	23.86764	K562	ZNF622
chr6	136579727	136579841	-	3'-UTR,intron-Distal,intron-Proximal	4.59004	23.06337	HepG2	KHSRP
chr6	136590601	136590686	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.22959	22.64722	K562	UCHL5
chr6	136590526	136590582	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.34572	22.02483	K562	AQR
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 221 entries

The BCLAF1 protein binds to its own RNA.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
BCLAF1	chr6	136599658	136599745	-	4.31398	17.96214	HepG2	BCLAF1	CDS,intron-Distal
BCLAF1	chr6	136599745	136599806	-	4.18629	16.40391	HepG2	BCLAF1	CDS,intron-Distal
BCLAF1	chr6	136590681	136590743	-	4.47755	15.55920	HepG2	BCLAF1	3'-UTR,CDS,intron-Distal,intron-Proximal
BCLAF1	chr6	136599010	136599065	-	3.62917	13.63814	HepG2	BCLAF1	CDS,intron-Distal
BCLAF1	chr6	136593136	136593213	-	4.19783	13.37200	HepG2	BCLAF1	3'-UTR,CDS,intron-Distal
BCLAF1	chr6	136596998	136597060	-	3.65394	13.10126	HepG2	BCLAF1	3'-UTR,CDS,intron-Distal
BCLAF1	chr6	136590617	136590681	-	3.76274	13.09328	HepG2	BCLAF1	3'-UTR,CDS,intron-Distal,intron-Proximal
BCLAF1	chr6	136588183	136588243	-	3.31409	12.85546	HepG2	BCLAF1	3'-UTR,CDS,intron-Distal
BCLAF1	chr6	136599415	136599477	-	3.33537	11.76394	HepG2	BCLAF1	CDS,intron-Distal
BCLAF1	chr6	136582158	136582237	-	4.15011	10.67609	HepG2	BCLAF1	3'-UTR,intron-Distal,intron-Proximal

Showing 1 to 10 of 24 entries

Diffrential Expression

7,223 differentially expressed genes after Knockdown/Knockout BCLAF1 protein.

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104 RBPs can affect the levels of BCLAF1.

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7,223 differentially expressed genes after Knockdown/Knockout BCLAF1 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
ANLN	ENSG00000011426.6	723.17090	2137.27300	-1.56340	32.67130	30.18300	HepG2	BCLAF1
NCAPD2	ENSG00000010292.8	1285.24280	3224.00380	-1.32680	28.40310	26.05920	HepG2	BCLAF1
TFPI	ENSG00000003436.10	8880.13130	4819.08200	0.88170	16.07830	14.20190	HepG2	BCLAF1
SRCIN1	ENSG00000017373.11	360.16210	87.78670	2.03650	15.52430	13.67500	HepG2	BCLAF1
TACC3	ENSG00000013810.14	398.43820	1013.10760	-1.34640	14.37410	12.56960	HepG2	BCLAF1
BRCA1	ENSG00000012048.15	393.00100	871.80680	-1.14950	14.03330	12.23930	HepG2	BCLAF1
FKBP4	ENSG00000004478.5	753.03190	1506.78810	-1.00070	13.83130	12.04540	HepG2	BCLAF1
CYP24A1	ENSG00000019186.5	287.58580	636.61330	-1.14640	12.50300	10.77920	HepG2	BCLAF1
C1orf112	ENSG00000000460.12	129.74390	337.81670	-1.38060	11.10010	9.45610	HepG2	BCLAF1
CHDH	ENSG00000016391.6	3389.39170	1906.58710	0.82990	10.89320	9.26050	HepG2	BCLAF1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

104 RBPs can affect the gene expression of BCLAF1 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
BCLAF1	ENSG00000029363.11	8987.84900	3616.98420	1.31310	98.74620	96.88340	HepG2	SRSF1
BCLAF1	ENSG00000029363.11	14322.09930	5244.36550	1.44930	48.86390	46.49290	K562	PUS1
BCLAF1	ENSG00000029363.11	9728.33730	5109.98140	0.92880	37.00110	34.75110	K562	RBM15
BCLAF1	ENSG00000029363.11	16976.52540	8027.06140	1.08050	30.61590	28.85890	K562	AQR
BCLAF1	ENSG00000029363.11	6458.46200	3361.35340	0.94200	28.44320	25.48670	K562	RCC2
BCLAF1	ENSG00000029363.11	3222.68590	6758.08880	-1.06840	25.47000	23.64760	HepG2	CPSF6
BCLAF1	ENSG00000029363.11	7873.72460	5082.20570	0.63150	24.88840	23.29780	K562	AGGF1
BCLAF1	ENSG00000029363.11	7823.76830	3875.31560	1.01340	24.00530	21.78910	K562	PRPF8
BCLAF1	ENSG00000029363.11	2952.40730	4505.82540	-0.60990	20.56540	18.02040	K562	BCLAF1
BCLAF1	ENSG00000029363.11	4480.35750	6424.16950	-0.51990	15.70430	14.30410	HepG2	AUH
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 125 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

149 alternative splicing events after Knockdown/Knockout BCLAF1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SNRNP70	ENSG00000104852.10	chr19	+	49605370	49606844	49604646	49604728	49607890	49607992	0.22100	0.00000	included	HepG2	BCLAF1
SE	PLOD2	ENSG00000152952.7	chr3	-	145795648	145795711	145794568	145794682	145796902	145797044	0.19000	0.00000	included	HepG2	BCLAF1
SE	ALDOA	ENSG00000149925.12	chr16	+	30077145	30077248	30075781	30075826	30078554	30078654	0.10400	0.00195	included	HepG2	BCLAF1
SE	SEC31A	ENSG00000138674.12	chr4	-	83752089	83752128	83750152	83750211	83763292	83763634	0.44900	0.00000	included	HepG2	BCLAF1
SE	KHK	ENSG00000138030.8	chr2	+	27317667	27317802	27315199	27315316	27319596	27319669	0.64000	0.00000	included	HepG2	BCLAF1
SE	OSBPL9	ENSG00000117859.14	chr1	+	52221991	52222030	52215816	52215867	52226361	52226452	0.21900	0.00124	included	HepG2	BCLAF1
SE	COBLL1	ENSG00000082438.11	chr2	-	165583615	165583654	165578934	165579032	165584478	165584707	0.24000	0.00155	included	HepG2	BCLAF1
SE	GPR126	ENSG00000112414.10	chr6	+	142762044	142762090	142759348	142759501	142764473	142767403	0.20900	0.00000	included	HepG2	BCLAF1
SE	RBM39	ENSG00000131051.16	chr20	-	34301626	34301779	34300940	34301018	34302106	34302311	0.10700	0.00045	included	HepG2	BCLAF1
SE	CHKA	ENSG00000110721.7	chr11	-	67828959	67829297	67820981	67821514	67829419	67829501	0.06400	0.00085	included	HepG2	BCLAF1

Showing 1 to 10 of 100 entries

49 RBPs can affect the splicing of BCLAF1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	BCLAF1	ENSG00000029363.11	chr6	-	136597605	136597646	136596980	136597127	136599002	136599241	0.25000	0.00000	included	K562	SRSF1
SE	BCLAF1	ENSG00000029363.11	chr6	-	136590278	136590441	136589299	136589477	136590574	136590750	-0.22400	0.00000	excluded	K562	SRSF1
SE	BCLAF1	ENSG00000029363.11	chr6	-	136588166	136588313	136582402	136582615	136589299	136589477	-0.16400	0.00000	excluded	K562	SRSF1
SE	BCLAF1	ENSG00000029363.11	chr6	-	136590278	136590441	136589299	136589477	136590574	136590750	-0.16000	0.00001	excluded	K562	PRPF8
SE	BCLAF1	ENSG00000029363.11	chr6	-	136588166	136588313	136582402	136582615	136589299	136589477	-0.27900	0.00000	excluded	K562	PRPF8
SE	BCLAF1	ENSG00000029363.11	chr6	-	136588166	136588313	136582402	136582615	136589299	136589477	-0.30700	0.00000	excluded	HepG2	U2AF1
SE	BCLAF1	ENSG00000029363.11	chr6	-	136588166	136588313	136582402	136582615	136589299	136589477	-0.20000	0.00000	excluded	HepG2	HNRNPM
SE	BCLAF1	ENSG00000029363.11	chr6	-	136590278	136590441	136589299	136589477	136590574	136590750	-0.13300	0.00061	excluded	K562	XRCC6
SE	BCLAF1	ENSG00000029363.11	chr6	-	136588166	136588313	136582402	136582615	136589299	136589477	-0.19800	0.00000	excluded	HepG2	DKC1
SE	BCLAF1	ENSG00000029363.11	chr6	-	136588166	136588313	136582402	136582615	136589299	136589477	-0.11700	0.00000	excluded	HepG2	TBRG4

Showing 1 to 10 of 102 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The BCLAF1 protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
SE	BCLAF1	ENSG00000029363.11	chr6	-	136590278	136590441			136589299	136589477	136590574	136590750	-0.08400	0.00000	0.00342	excluded	HepG2	BCLAF1

Showing 1 to 1 of 1 entries