BCLAF1 antibody: A300-608A


Antibody Info
Product_IDA300-608A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB012FUX
Target Info
GeneNameBCLAF1
Phosphate
Target_nameBCLAF1-human
Antibody Info
Product_IDA300-608A
Lot_ID2
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_ID
Target Info
GeneNameBCLAF1
Phosphate
Target_nameBCLAF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A300-608A_1_BCLAF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-BCLAF1 antibody (lanes under 'BCLAF1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-BCLAF1 antibody (lanes under 'BCLAF1').

Method: immunoprecipitation

Releated Sample: eCLIP:643

Status: Released

Lab: Yeo Lab

 BCLAF1_Secondary_Western.png<br>Caption: Western blot following shRNA against BCLAF1 in K562 and HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against BCLAF1. Lanes 5-8 follow the same pattern, but in HepG2. BCLAF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against BCLAF1 in K562 and HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against BCLAF1. Lanes 5-8 follow the same pattern, but in HepG2. BCLAF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV19-7

Status: Released

Lab: Graveley Lab

K562 BCLAF1_Secondary_Western.png<br>Caption: Western blot following shRNA against BCLAF1 in K562 and HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against BCLAF1. Lanes 5-8 follow the same pattern, but in HepG2. BCLAF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against BCLAF1 in K562 and HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against BCLAF1. Lanes 5-8 follow the same pattern, but in HepG2. BCLAF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV19-7

Status: Released

Lab: Graveley Lab

Cell linePrimary validationSecondary validation
K562 BCLAF1-K562-CRISPR-A300-608A.png<br>Caption: Western blot following CRISPR against BCLAF1 in K562 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BCLAF1. BCLAF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against BCLAF1 in K562 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BCLAF1. BCLAF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGKcLV40-3

Status: Submitted

Lab: Graveley Lab