EEF2

General Information

Gene Name : EEF2
Gene ID : ENSG00000167658.15
Essential Gene : Yes

Functions

Ribosome & basic translation
Translation regulation

Localization(RBPImage: EEF2)

Cytoplasm
Cytosolic
Mitotic-Apparatus (Spindle; Centrosomes)

Domains (AlphaFold: EEF2)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all EEF2 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	EEF2	ENSG00000167658.15	1028.96	1660.66	1344.81	ENCSR000AEF
HepG2	EEF2	ENSG00000167658.15	1417.00	954.08	1185.54	ENCSR985KAT
K562	EEF2	ENSG00000167658.15	1084.57	1598.15	1341.36	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 4037	ENCSR004VIR	EEF2	K562	Released	ENCAB559BYO	ENCSR344GAQ
knockdown followed by RNA-seq	EEF2-BGKLV19-K562	ENCSR181RLB	EEF2	K562	Released		ENCSR661HEL	shRNA	KD% = 62.7,68.9
knockdown followed by RNA-seq	EEF2-BGHLV16-HepG2	ENCSR477TRX	EEF2	HepG2	Released		ENCSR689PHN	shRNA	KD% = 69.9,59.3

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
EEF2	4037	K562	ENCSR004VIR	ENCSR344GAQ	ENCAB559BYO	711842	1931156	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
EEF2	ENCAB334DWC	A301-688A	1	Released, ENCODE3	Released, ENCODE3	BGHLV16-19, Released, ENCODE3	BGKLV19-21, Released, ENCODE3
EEF2	ENCAB828EJY	702807	2000400	Released, ENCODE3	Released, ENCODE4	BGHLV16-19, Released, ENCODE3	BGKLV19-21, Released, ENCODE4
EEF2	ENCAB559BYO	711842	1931156	Released, ENCODE3	Released, ENCODE4	BGHLV16-19, Released, ENCODE3	BGKLV19-21, Released, ENCODE4
EEF2	ENCAB152SCA	711843	1954520	Released, ENCODE3	Released, ENCODE4	BGHLV16-19, Released, ENCODE3
EEF2	ENCAB244LPV	200559	CD0822	Released, ENCODE3	Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
EEF2	K562	shRNA	ENCSR181RLB	ENCSR661HEL	EEF2_BGKLV19_21B,EEF2_BGKLV19_22B	NT_BGKLV19_1B,NT_BGKLV19_2B	62.7,68.9	Released	ENCODE3
EEF2	HepG2	shRNA	ENCSR477TRX	ENCSR689PHN	EEF2_BGHLV16_19,EEF2_BGHLV16_20	NT_BGHLV16_1,NT_BGHLV16_2	69.9,59.3	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

EEF2: Click to RBP Image Database

Cytoplasm

Cytosolic

Mitotic-Apparatus (Spindle; Centrosomes)

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
EEF2	Product_ID: A301-688A Lot_ID: 1 Source: Bethyl Labs Target Name: EEF2-human
EEF2	Product_ID: 711843 Lot_ID: 1954520 Source: Thermo Fisher Target Name: EEF2-human
EEF2	Product_ID: 702807 Lot_ID: 2000400 Source: Thermo Fisher Target Name: EEF2-human
EEF2	Product_ID: 711842 Lot_ID: 1931156 Source: Thermo Fisher Target Name: EEF2-human
EEF2	Product_ID: 200559 Lot_ID: CD0822 Source: Zen Bioscience Target Name: EEF2-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for EEF2 is not avaliable on ENCORE database.

Proteins bound to RNA

EEF2 RNA is bound by 49 proteins.

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eCLIP assay for EEF2 is not avaliable on ENCORE database.

49 proteins are bound to EEF2 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr19	3976501	3976557	-	3'-UTR,CDS,intron-Distal	4.64494	400.00000	K562	METAP2
chr19	3976172	3976212	-	3'-UTR,CDS,intron-Distal	4.69642	400.00000	HepG2	SUB1
chr19	3976147	3976185	-	3'-UTR,CDS,intron-Distal	4.11309	400.00000	K562	IGF2BP2
chr19	3976273	3976315	-	3'-UTR,CDS,intron-Distal	3.78481	400.00000	K562	GTF2F1
chr19	3976334	3976372	-	3'-UTR,intron-Distal	3.50544	400.00000	K562	PABPC4
chr19	3976274	3976296	-	3'-UTR,CDS,intron-Distal	3.62009	400.00000	K562	PABPC4
chr19	3976386	3976434	-	3'-UTR,intron-Distal	4.20623	400.00000	K562	IGF2BP2
chr19	3976330	3976368	-	3'-UTR,intron-Distal	4.73844	400.00000	K562	IGF2BP2
chr19	3976185	3976210	-	3'-UTR,CDS,intron-Distal	4.28623	400.00000	K562	IGF2BP2
chr19	3976251	3976269	-	3'-UTR,CDS,intron-Distal	3.46905	400.00000	K562	PABPC4
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 348 entries

eCLIP assay for EEF2 is not avaliable on ENCORE database.

Diffrential Expression

5,100 differentially expressed genes after Knockdown/Knockout EEF2 protein.

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121 RBPs can affect the levels of EEF2.

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5,100 differentially expressed genes after Knockdown/Knockout EEF2 protein :

Only display 100 entries as example. Please click "View All" button to see all 5,100 entries.

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Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
TFPI	ENSG00000003436.10	4090.24170	8919.98030	-1.12490	27.21650	24.91150	HepG2	EEF2
SLC7A2	ENSG00000003989.12	1350.31710	5509.63490	-2.02870	22.68950	20.51590	HepG2	EEF2
CD74	ENSG00000019582.10	3520.57730	1566.18410	1.16850	18.75670	16.69690	HepG2	EEF2
JHDM1D	ENSG00000006459.6	629.03110	2218.96110	-1.81870	18.46740	16.42840	HepG2	EEF2
DPEP1	ENSG00000015413.5	630.34570	185.31570	1.76610	14.66900	12.80740	HepG2	EEF2
C8B	ENSG00000021852.8	696.57200	298.71280	1.22140	14.65450	12.79550	HepG2	EEF2
MVP	ENSG00000013364.14	1299.33670	675.12130	0.94450	12.71770	10.95460	HepG2	EEF2
RB1CC1	ENSG00000023287.8	1135.08300	1927.15700	-0.76370	9.97150	8.36170	HepG2	EEF2
SPATA20	ENSG00000006282.15	1875.00070	1088.98920	0.78380	9.96120	8.35210	HepG2	EEF2
MLXIPL	ENSG00000009950.11	4494.40450	2542.67340	0.82170	9.60420	8.01720	HepG2	EEF2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

121 RBPs can affect the gene expression of EEF2 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
EEF2	ENSG00000167658.11	26046.41060	90757.00230	-1.80090	267.40070	264.79950	HepG2	SRSF1
EEF2	ENSG00000167658.11	62973.65070	29371.76950	1.10020	126.95220	124.00440	HepG2	SLBP
EEF2	ENSG00000167658.11	43135.45010	17569.70590	1.29570	125.03470	122.07640	HepG2	SAFB2
EEF2	ENSG00000167658.11	47273.77380	19342.62660	1.28920	113.71300	110.80280	HepG2	TRIM56
EEF2	ENSG00000167658.11	55539.57490	29476.91510	0.91380	93.67440	90.80260	HepG2	HNRNPL
EEF2	ENSG00000167658.11	44210.25710	92944.93750	-1.07200	83.20880	81.29720	HepG2	NELFE
EEF2	ENSG00000167658.11	40972.04000	83517.06190	-1.02740	64.35820	62.53770	HepG2	PTBP1
EEF2	ENSG00000167658.11	53019.32490	32517.06080	0.70520	58.19500	56.22030	HepG2	PUM1
EEF2	ENSG00000167658.11	38105.06260	81579.68140	-1.09820	49.57830	47.59290	HepG2	TAF15
EEF2	ENSG00000167658.11	37782.97990	20153.08360	0.90660	45.51970	43.09000	HepG2	ILF2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 146 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for EEF2 is not available on ENCORE database.

9 RBPs can affect the splicing of EEF2 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
A5SS	EEF2	ENSG00000167658.11	chr19	-	3982243	3982422			3982342	3982422	3981336	3981450	-0.82800	0.00000	0.00017	shorter	K562	RBM15
MXE	EEF2	ENSG00000167658.11	chr19	-	3981336	3981450	3981944	3982050	3980684	3980977	3982243	3982311	0.10800	0.00002	0.00181	1included	K562	DHX30
A3SS	EEF2	ENSG00000167658.11	chr19	-	3979326	3979575			3979326	3979511	3979805	3980064	0.37800	0.00006	0.00057	longer	K562	AQR
MXE	EEF2	ENSG00000167658.11	chr19	-	3981336	3981450	3981944	3982050	3980684	3980977	3982243	3982311	-0.08700	0.00057	0.01570	2included	K562	SUPV3L1
A3SS	EEF2	ENSG00000167658.11	chr19	-	3979326	3979575			3979326	3979511	3979805	3980064	0.29700	0.00145	0.06459	longer	K562	PUS1
MXE	EEF2	ENSG00000167658.11	chr19	-	3981336	3981450	3981944	3982050	3980684	3980977	3982243	3982311	-0.08000	0.00151	0.09530	2included	K562	NONO
MXE	EEF2	ENSG00000167658.11	chr19	-	3981336	3981450	3981944	3982050	3980684	3980977	3982243	3982311	-0.08100	0.00167	0.07219	2included	K562	TIA1
MXE	EEF2	ENSG00000167658.11	chr19	-	3981336	3981450	3981944	3982050	3980684	3980977	3982243	3982311	-0.07800	0.00234	0.03956	2included	K562	HNRNPC
MXE	EEF2	ENSG00000167658.11	chr19	-	3981336	3981450	3981944	3982050	3980684	3980977	3982243	3982311	-0.08600	0.01006	0.09702	2included	K562	HNRNPUL1

Showing 1 to 9 of 9 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

EEF2

General Information

Functions

Localization(RBPImage: EEF2)

Domains (AlphaFold: EEF2)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for EEF2 is not avaliable on ENCORE database.

49 proteins are bound to EEF2 RNA:

eCLIP assay for EEF2 is not avaliable on ENCORE database.

Diffrential Expression

5,100 differentially expressed genes after Knockdown/Knockout EEF2 protein :

121 RBPs can affect the gene expression of EEF2 :

The Knockdown/knockout RNA-Seq assay for EEF2 is not available on ENCORE database.

9 RBPs can affect the splicing of EEF2 :

The Knockdown/knockout RNA-Seq assay for EEF2 is not avaliable on ENCORE database.