Antibodies of EEF2


5 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EEF2Product_ID: A301-688A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EEF2-human		 HepG2_Bethyl_A301-688A_1_EEF2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EEF2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EEF2 antibody (lanes under 'EEF2').
 EEF2_Secondary_Western.png<br>Caption: Western blot following shRNA against EEF2 in K562 and HepG2 whole cell lysate using EEF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EEF2. Lanes 5-7 follow the same pattern, but in HepG2. EEF2 protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_Bethyl_A301-688A_1_EEF2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EEF2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EEF2 antibody (lanes under 'EEF2').
 EEF2_Secondary_Western.png<br>Caption: Western blot following shRNA against EEF2 in K562 and HepG2 whole cell lysate using EEF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EEF2. Lanes 5-7 follow the same pattern, but in HepG2. EEF2 protein appears as the green band, GAPDH serves as a control and appears in red.
EEF2Product_ID: 711843
Lot_ID: 1954520
Source: Thermo Fisher
Target Name: EEF2-human		 ThermoFisher_711843_1954520_EEF2%20pThr56.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EEF2 pThr56 specific antibody, 711843. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EEF2 pThr56-specific antibody, 711843. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
EEF2Product_ID: 702807
Lot_ID: 2000400
Source: Thermo Fisher
Target Name: EEF2-human		 ThermoFisher_702807_2000400_EEF2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EEF2 specific antibody, 702807. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EEF2-specific antibody, 702807. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EEF2-K562-702807.png<br>Caption: Western blot following shRNA against EEF2 in K562 whole cell lysate using EEF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EEF2. EEF2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
EEF2Product_ID: 711842
Lot_ID: 1931156
Source: Thermo Fisher
Target Name: EEF2-human		 ThermoFisher_711842_1931156_EEF2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EEF2 specific antibody, 711842. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EEF2-specific antibody, 711842. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EEF2-K562-711842.png<br>Caption: Western blot following shRNA against EEF2 in K562 whole cell lysate using EEF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EEF2. EEF2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
EEF2Product_ID: 200559
Lot_ID: CD0822
Source: Zen Bioscience
Target Name: EEF2-human		 EEF2_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using eEF2 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 30% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 30% IP enrichment using mouse monoclonal anti-eEF2 antibody (lanes under 'anti-eEF2'). Asterisk indicates heavy and light chains of antibody.