BCLAF1 RNA-Seq in HepG2

BCLAF1 CRISPR in HepG2 Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information


RBP	BCLAF1
Cell_Line	HepG2
Method	CRISPR
Exp_Name	BCLAF1-BGHcLV41-HepG2
ENCODE_series_ID
Batch_ID	BGHcLV41
Pool ID	Pool-240314
Local_Set_Name	set70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1	BCLAF1-BGHcLV41-3
Rep2	BCLAF1-BGHcLV41-4
CN1	NT-BGHcLV41-1
CN2	NT-BGHcLV41-2
Rep1_qPCR	69.5
Rep2_qPCR	76.2
Rep1_WB	need discussion
Rep2_WB
Antibody Cat#	A300-608A
Antibody Lot#	lot # 2
Antibody DCC ID
Status	Submitted
Project	ENCORE2
ID	1445

Experiment Information (Status: Submitting)

	BGHcLV41-3	BGHcLV41-4
idx	1270	1271
TRCN#_or_BGC#	BGC#0000892	BGC#0000892
shRNA_or_gRNA_sequence	TACCTGTTAGAATCATCAAG	TACCTGTTAGAATCATCAAG
PAM	AGG	AGG
Name	BCLAF1_69	BCLAF1_69
Sample_ID	BGHcLV41-3	BGHcLV41-4
transduction_Date	1/12/24	1/12/24
days	D6	D6
RBP_name	BCLAF1	BCLAF1
qPCR_result	69.5	76.2
Ave_qPCR	72.9
RT-qPCR_primer-F	aatggcacctgttcctcttg
RT-qPCR_primer-R	ggatcggaattcttgctcct
protein_conc	3268	3095
WB_result	need discussion
Ave_WB
WB_DONE_date	1/
MW	106kd
IP
antibody_Cat#	A300-608A	lot # 2
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note	check result befor release	check result befor release
ID	9509	9510

Western Blot Western Blot info was not avaliable

Experiment Status

	BGHcLV41-3	BGHcLV41-4
Sample_ID	BGHcLV41-3	BGHcLV41-4
Sample Name
Sample Name	BCLAF1-BGHcLV41-3	BCLAF1-BGHcLV41-4
RBP	BCLAF1	BCLAF1
Cell_Line	HepG2	HepG2
Exp UID
Status	Submitting	Submitting
Status_date	2024-09-20	2024-09-20
Project	ENCORE2	ENCORE2
Note
ID	12950	12951

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV41-3	BGHcLV41-4
Sample #	3	4
Sample Name	BCLAF1-BGHcLV41-3	BCLAF1-BGHcLV41-4
Sample_Name_Alias
Index Well Position	C01	D01
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2024-03-01	2024-03-01
Lib_ID	Lib-240301	Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp	302	299
Peak_Molarity	68.70	87.10
libSampleQC_DNA_Well	DNA_Library_set70/set1/C1.png	DNA_Library_set70/set1/D1.png
RIN	9.6	9.4
libSample_RNA_Well	RNA_Library_set70/set1/G6.png	RNA_Library_set70/set1/H6.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-03-14	2024-03-14
Sample_ID	BGHcLV41-3	BGHcLV41-4
RBP	BCLAF1	BCLAF1
Batch_ID	BGHcLV41	BGHcLV41
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4228	4229

Sequencing Information

	BGHcLV41-3	BGHcLV41-4
Sample_ID	BGHcLV41-3	BGHcLV41-4
Sample Name	BCLAF1-BGHcLV41-3	BCLAF1-BGHcLV41-4
Pool ID	Pool-240314	Pool-240314
LocalServer_folder	set70	set70
total_reads	27,296,640	35,947,359
total_aligned_reads	23,484,522	32,508,453
unique_aligned_reads	22,207,780	30,678,342
percent_uniqueAligned	0.81357	0.85342
correlation_replicates	0.991605	0.991605
spikein_reads	6,054	1,252
percent_spikeins	0.00022	0.00003
original_ReadLength	151	151
QC_Status	Submitting	Submitting
ID	2947	2948

Data Submission Information DCC submission information was not avaliable

File Information Files of the experiment was not avaliable in the database