RBM8A

General Information

Gene Name       : RBM8A
Gene ID             : ENSG00000265241.6
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RBM8A)


Antibody Validation

 View validation images for all RBM8A antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RBM8AENSG00000265241.6120.71178.13149.42ENCSR000AEF
HepG2RBM8AENSG00000265241.6181.81171.44176.62ENCSR985KAT
K562RBM8AENSG00000265241.6173.28218.07195.68ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RBM8A-BGKcLV30-K562ENCSR163RXIRBM8AK562ReleasedENCSR347NKX   CRISPRKD% = 86.5,88.1






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RBM8AENCAB121MYBA301-033A1Released, ENCODE4BGHcLV34-31, Released, ENCODE4BGKcLV30-23, Released, ENCODE4
RBM8AENCAB654PTXRN120PW1Released, ENCODE3BGHcLV34-31, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RBM8AK562CRISPRENCSR163RXIENCSR347NKXRBM8A-BGKcLV30-23,RBM8A-BGKcLV30-24NT-BGKcLV30-1,NT-BGKcLV30-286.5,88.1ReleasedENCODE4
RBM8AHepG2CRISPRBGHcLV32-21,BGHcLV32-22BGHcLV32-1,BGHcLV32-236.0,41.7NotSatisfiedENCODE4
RBM8AHepG2CRISPRRBM8A-BGHcLV34-31,RBM8A-BGHcLV34-32NT-BGHcLV34-1,NT-BGHcLV34-270.8,79.8SubmittingENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM8AProduct_ID: A301-033A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM8A-human		 RBM8A-HEPG2-CRISPR-A301-033A.png<br>Caption: Western blot following CRISPR against RBM8A in HepG2 whole cell lysate using RBM8A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM8A. RBM8A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-033A_1_RBM8A.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM8A specific antibody, A301-033A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM8A-specific antibody, A301-033A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM8A-K562-CRISPR-A301-033A.png<br>Caption: Western blot following CRISPR against RBM8A in K562 whole cell lysate using RBM8A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM8A. RBM8A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RBM8AProduct_ID: RN120PW
Lot_ID: 1
Source: MBLI
Target Name: RBM8A-human		 K562_MBLI_RN120PW_001_RBM8A.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM8A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM8A antibody (lanes under 'RBM8A').








RNA Targets

  • eCLIP assay for RBM8A is not avaliable on ENCORE database.



Proteins bound to RNA
  • RBM8A RNA is bound by 20 proteins.  







eCLIP assay for RBM8A is not avaliable on ENCORE database.





20 proteins are bound to RBM8A RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1145509281145509350+3'-UTR4.237106.73573K562PUM2
chr1145510715145510804+3'-UTR4.8794618.26619K562UPF1
chr1145509305145509358+3'-UTR3.358588.95130K562KHSRP
chr1145511333145511388+3'-UTR3.976834.66555K562FAM120A
chr1145509304145509345+3'-UTR4.193247.37833K562PABPC4
chr1145510899145510932+3'-UTR5.045377.60978K562UPF1
chr1145511312145511374+3'-UTR4.437518.17454K562UPF1
chr1145510960145511062+3'-UTR3.242785.14369K562FAM120A
chr1145510961145511034+3'-UTR5.2191016.13372K562UPF1
chr1145510985145511047+3'-UTR4.511093.18753K562AKAP1
chr1145509646145509696+3'-UTR3.504146.67761K562UPF1
chr1145510894145511036+3'-UTR3.631155.73766K562YBX3
chr1145510344145510392+3'-UTR5.4240917.33080K562UPF1
chr1145510297145510344+3'-UTR5.3786618.13060K562UPF1
chr1145510517145510540+3'-UTR3.887624.50550K562UPF1
chr1145508051145508077+CDS,intron-Proximal5.1242622.84851K562UCHL5
chr1145508915145508939+CDS,intron-Proximal3.128374.88466K562UCHL5
chr1145508475145508539+CDS3.086346.33149K562RBM15
chr1145508940145509016+CDS4.390294.01763HepG2GRWD1
chr1145508552145508606+CDS3.006703.01032HepG2LARP4
chr1145508247145508300+CDS,intron-Proximal3.780524.28282K562EFTUD2
chr1145508561145508609+CDS3.284126.37614K562GRWD1
chr1145507675145507727+CDS4.059695.97027HepG2GRWD1
chr1145508054145508105+CDS,intron-Proximal3.159256.18341HepG2PRPF8
chr1145508189145508265+CDS,intron-Proximal3.221474.16067K562XRCC6
chr1145508968145509006+CDS3.302206.02234K562UCHL5
chr1145507727145507733+CDS4.509304.21087HepG2GRWD1
chr1145508023145508083+CDS,intron-Proximal3.576128.16971K562EFTUD2
chr1145508200145508239+CDS,intron-Proximal3.3854411.99289K562ZNF622
chr1145507989145508053+CDS,intron-Proximal4.378838.92943HepG2AQR
chr1145508235145508284+CDS3.204284.89942HepG2GRWD1
chr1145507683145507733+CDS4.8562619.91188K562UCHL5
chr1145508160145508284+CDS,intron-Proximal3.9261320.38079K562UCHL5
chr1145508251145508289+CDS,intron-Proximal4.609525.52476K562BUD13
chr1145508189145508247+CDS,intron-Proximal4.2109512.20650K562EFTUD2
chr1145508014145508043+CDS,intron-Proximal3.313244.79429HepG2GRWD1
chr1145508487145508548+CDS3.207496.83963HepG2GRWD1
chr1145508957145509052+CDS5.101227.26512HepG2LARP4
chr1145509154145509197+CDS,intron-Proximal3.178967.40378K562BUD13
chr1145508014145508051+CDS,intron-Proximal4.3630823.49461K562UCHL5
chr1145508015145508051+CDS,intron-Proximal3.0821316.82950K562ZNF622
chr1145508486145508544+CDS3.026307.04888K562UCHL5
chr1145508051145508076+CDS,intron-Proximal3.5087113.05000K562ZNF622
chr1145508203145508242+CDS,intron-Proximal3.669719.72262K562GRWD1
chr1145508205145508244+CDS,intron-Proximal3.459145.07480K562PUM1
chr1145508015145508080+CDS,intron-Proximal3.259914.09016K562DDX6
chr1145508181145508251+CDS,intron-Proximal3.491189.65971K562BUD13
chr1145508114145508144+intron-Proximal3.746075.03105K562BUD13
chr1145508121145508166+intron-Proximal4.288113.89565K562PRPF8
chr1145508626145508698+intron-Proximal5.153495.70555K562PRPF8
chr1145508110145508189+intron-Proximal6.9400817.10436K562EFTUD2
chr1145508421145508471+intron-Proximal3.597497.00923K562SF3B4
chr1145508611145508689+intron-Proximal3.692388.77060K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RBM8A is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RBM8A is not available on ENCORE database.

  • 79 RBPs can affect the levels of RBM8A.  








The Knockdown/knockout RNA-Seq assay for RBM8A is not available on ENCORE database.





79 RBPs can affect the gene expression of RBM8A :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RBM8AENSG00000131795.81885.077201398.404500.430708.187807.25540HepG2ACO1
RBM8AENSG00000131795.83496.023001956.345800.8375010.767809.78530HepG2AKAP1
RBM8AENSG00000131795.81068.216801618.76560-0.599704.789003.76830HepG2ASCC1
RBM8AENSG00000131795.81854.336602211.65100-0.254203.163902.43650HepG2AUH
RBM8AENSG00000131795.81068.516601405.08940-0.395102.512901.65600HepG2BCLAF1
RBM8AENSG00000131795.81600.95320989.098300.694604.696003.69460HepG2CNOT7
RBM8AENSG00000131795.81114.150002459.21330-1.1423023.7286021.95980HepG2CPSF6
RBM8AENSG00000131795.81196.028502246.22200-0.9092026.9989025.41890HepG2CELF1
RBM8AENSG00000131795.81977.358502415.11680-0.288504.904103.95900HepG2DAZAP1
RBM8AENSG00000131795.81974.339502792.61410-0.500202.460301.53880HepG2DDX59
RBM8AENSG00000131795.81064.999101279.53940-0.264802.425101.76130HepG2DNAJC21
RBM8AENSG00000131795.81061.661801268.30210-0.256602.935102.26640HepG2DNAJC2
RBM8AENSG00000131795.81817.116301424.375900.351202.680701.77090HepG2EEF2
RBM8AENSG00000131795.81249.860901547.67390-0.308301.941201.43430HepG2EIF2S1
RBM8AENSG00000131795.81521.428001156.296700.395803.199902.23380HepG2EIF3D
RBM8AENSG00000131795.81862.672402382.76720-0.355305.433504.32720HepG2EIF4G1
RBM8AENSG00000131795.81242.541002282.51940-0.8773014.8250013.05670HepG2EIF4G2
RBM8AENSG00000131795.82058.445802539.27590-0.302904.369503.64410HepG2ETF1
RBM8AENSG00000131795.81877.695001366.544600.458305.400704.23510HepG2EWSR1
RBM8AENSG00000131795.82047.128402787.73640-0.445503.728602.99460HepG2GPKOW
RBM8AENSG00000131795.81507.608102218.47590-0.5573011.3966010.07440HepG2HNRNPF
RBM8AENSG00000131795.82046.449701620.458300.336602.748802.05400HepG2HNRNPK
RBM8AENSG00000131795.81864.823602199.94920-0.238403.310402.57840HepG2HNRNPL
RBM8AENSG00000131795.81541.035902660.38580-0.7877011.7072010.52670HepG2HNRNPLL
RBM8AENSG00000131795.81724.930901381.471200.320203.276702.25020HepG2HNRNPM
RBM8AENSG00000131795.81456.568802184.58370-0.584809.067307.82600HepG2IGF2BP1
RBM8AENSG00000131795.81153.416301627.23950-0.496504.405103.56960HepG2IGF2BP2
RBM8AENSG00000131795.81082.971501278.35460-0.239302.673101.66880HepG2KIF1C
RBM8AENSG00000131795.81906.261402394.83150-0.329203.832402.93400HepG2NOL12
RBM8AENSG00000131795.81146.140301582.49250-0.465403.746002.91440HepG2PABPC4
RBM8AENSG00000131795.81315.736701512.64250-0.201201.813601.30510HepG2PCBP1
RBM8AENSG00000131795.81908.477702286.11030-0.260502.048401.52330HepG2PUF60
RBM8AENSG00000131795.82021.565602426.84690-0.263604.370603.66200HepG2PUM1
RBM8AENSG00000131795.81627.313102425.04950-0.575509.725908.50740HepG2PUM2
RBM8AENSG00000131795.81333.693001666.91770-0.321803.262602.59230HepG2QKI
RBM8AENSG00000131795.82865.764102122.363800.433107.406506.65470HepG2RBM22
RBM8AENSG00000131795.81244.718101512.86700-0.281502.198401.59140HepG2RBM25
RBM8AENSG00000131795.81426.262901125.111600.342102.995802.36030HepG2RPS19
RBM8AENSG00000131795.8975.374401271.15600-0.382105.397004.55010HepG2SAFB2
RBM8AENSG00000131795.82774.910301935.902500.519303.959103.14660HepG2SF3A3
RBM8AENSG00000131795.81433.977902633.27170-0.8768016.1605014.60370HepG2SF3B1
RBM8AENSG00000131795.81113.304601602.44500-0.525409.152608.17320HepG2SF3B4
RBM8AENSG00000131795.81634.253702192.10530-0.423708.938407.79040HepG2SLBP
RBM8AENSG00000131795.81965.984501482.362400.407308.767407.72390HepG2SMN1
RBM8AENSG00000131795.82176.023502929.97100-0.429202.408801.68200HepG2SNRNP70
RBM8AENSG00000131795.83268.616502397.130200.4473010.9689010.15060HepG2SRFBP1
RBM8AENSG00000131795.82534.226101672.634800.5993015.7676014.83630HepG2SRSF1
RBM8AENSG00000131795.81773.137302179.51590-0.297703.470402.70360HepG2SRSF7
RBM8AENSG00000131795.81084.986601360.80260-0.326804.072303.42730HepG2SRSF9
RBM8AENSG00000131795.81244.785502081.27360-0.7416021.4312020.29870HepG2SSB
RBM8AENSG00000131795.82180.846502567.07280-0.235203.354002.68240HepG2SUCLG1
RBM8AENSG00000131795.81018.264901352.15250-0.409103.251602.23790HepG2SUPT6H
RBM8AENSG00000131795.81182.743001582.65880-0.420202.661001.99610HepG2SUPV3L1
RBM8AENSG00000131795.81876.473102592.98200-0.466605.118904.17750HepG2TARDBP
RBM8AENSG00000131795.82677.363101712.684300.644509.038907.89580HepG2U2AF1
RBM8AENSG00000131795.81853.363402364.16540-0.351202.219501.47440HepG2UBE2L3
RBM8AENSG00000131795.82301.568401657.552100.473509.689408.67890HepG2UTP3
RBM8AENSG00000131795.83360.477302839.708500.242803.438902.81890K562AGGF1
RBM8AENSG00000131795.83715.499502954.829500.330403.240402.53170K562AKAP8L
RBM8AENSG00000131795.85311.899303994.626500.411103.166602.54680K562AQR
RBM8AENSG00000131795.81914.650902440.91430-0.350306.027804.59080K562BCLAF1
RBM8AENSG00000131795.82113.058403906.78370-0.8866011.7153010.08440K562CPSF6
RBM8AENSG00000131795.86742.715904566.107100.5623013.8434012.49700K562DDX47
RBM8AENSG00000131795.83923.422103179.708100.303104.348003.21620K562DKC1
RBM8AENSG00000131795.82573.389701889.350300.445706.887605.64340K562EIF3G
RBM8AENSG00000131795.85771.497604919.254200.230403.404902.36650K562EIF4G2
RBM8AENSG00000131795.82701.367001782.718600.5995012.8203011.61550K562FXR1
RBM8AENSG00000131795.82852.096003730.13200-0.387203.372602.49840K562GPKOW
RBM8AENSG00000131795.81867.451702385.73580-0.353403.582602.91510K562HNRNPC
RBM8AENSG00000131795.82717.629302001.670800.441004.074302.53560K562HNRNPK
RBM8AENSG00000131795.83936.237203135.868500.327903.688002.71710K562KIF1C
RBM8AENSG00000131795.81696.478502126.76660-0.326103.012601.40360K562LSM11
RBM8AENSG00000131795.83700.508202462.323500.587605.116603.79260K562NAA15
RBM8AENSG00000131795.82953.686803635.10740-0.299506.142205.09000K562NSUN2
RBM8AENSG00000131795.82377.988301874.103100.343403.153702.10970K562PCBP2
RBM8AENSG00000131795.83156.050101971.999400.678409.925008.49870K562PRPF8
RBM8AENSG00000131795.86985.111505367.344100.380004.014003.03600K562PUF60
RBM8AENSG00000131795.85525.655802930.494300.9149018.4797017.10830K562PUS1
RBM8AENSG00000131795.83841.133202855.230500.427806.252705.38580K562RBM15
RBM8AENSG00000131795.82626.603601962.186800.420604.422203.32800K562RBM22
RBM8AENSG00000131795.81627.207201951.23070-0.262002.301701.40230K562RCC2
RBM8AENSG00000131795.82594.286002064.927000.329102.577801.52730K562NELFE
RBM8AENSG00000131795.84202.964403586.638800.228702.704101.89850K562SF3A3
RBM8AENSG00000131795.81363.054501849.74820-0.440503.793602.53700K562SRFBP1
RBM8AENSG00000131795.83802.990802006.292700.9225023.8212021.74380K562SRSF1
RBM8AENSG00000131795.81216.190701909.47940-0.650806.752305.11120K562SUPV3L1
RBM8AENSG00000131795.83519.498602480.533200.504608.452307.01480K562SYNCRIP
RBM8AENSG00000131795.82249.029201622.961300.470603.933802.58370K562U2AF1
RBM8AENSG00000131795.82010.707801676.165500.262402.404101.65530K562U2AF2
RBM8AENSG00000131795.82280.231403298.08420-0.532408.411606.84270K562WDR3
RBM8AENSG00000131795.84222.138203429.455200.299902.740601.89790K562WDR43
RBM8AENSG00000131795.83007.283601989.364700.596102.979501.32980K562XRCC6
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RBM8A is not available on ENCORE database.





14 RBPs can affect the splicing of RBM8A :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SERBM8AENSG00000131795.8chr1+145508206145508284145508015145508075145508474145508611-0.052000.000040.00154excludedK562SF3B4
SERBM8AENSG00000131795.8chr1+145508209145508284145508015145508075145508474145508611-0.051000.000380.00896excludedK562U2AF2
SERBM8AENSG00000131795.8chr1+145508206145508284145508015145508075145508474145508611-0.069000.000000.00000excludedK562TARDBP
A3SSRBM8AENSG00000131795.8chr1+1455088801455090521455089151455090521455083951455086110.061000.000000.00000longerK562AQR
A5SSRBM8AENSG00000131795.8chr1+145508015145508284145508015145508075145508474145508611-0.090000.000000.00000shorterK562TARDBP
A5SSRBM8AENSG00000131795.8chr1+145508015145508284145508015145508075145508474145508611-0.053000.000340.02349shorterK562U2AF2
A5SSRBM8AENSG00000131795.8chr1+145508015145508284145508015145508075145508474145508611-0.057000.000080.01115shorterK562SF3B4
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.134000.001320.02241includedK562SF3B4
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082091455082840.061000.007630.04451includedK562PUS1
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455080751455084741455086110.204000.006800.06369includedHepG2SF3A3
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.053000.000490.02272includedK562SF3B1
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.284000.000000.00034includedK562TAF15
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.106000.000000.00000includedHepG2U2AF2
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.154000.000000.00000includedK562AGGF1
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455082841455084741455086110.053000.000000.00000includedK562AGGF1
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455080751455084741455086110.135000.001430.01524includedK562AKAP8L
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.057000.000000.00006includedHepG2TAF15
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.149000.000000.00000includedK562RBM15
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455082841455084741455086110.056000.000000.00000includedK562RBM15
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.294000.003820.05989includedK562U2AF1
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455082841455084741455086110.071000.000000.00000includedK562U2AF1
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.257000.000000.00003includedK562U2AF2
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455082841455084741455086110.077000.000000.00000includedK562U2AF2
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.099000.000000.00000includedHepG2SF3B4
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082061455082840.133000.000000.00000includedK562UTP18
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455082841455084741455086110.062000.000000.00000includedK562UTP18
RIRBM8AENSG00000131795.8chr1+145508015145508611145508015145508075145508474145508611-0.151000.000610.04796excludedK562FXR2
RIRBM8AENSG00000131795.8chr1+1455080151455082841455080151455080751455082091455082840.092000.000020.00015includedK562AQR
RIRBM8AENSG00000131795.8chr1+1455080151455086111455080151455082841455084741455086110.064000.000000.00000includedK562AQR
RIRBM8AENSG00000131795.8chr1+145508015145508611145508015145508075145508474145508611-0.560000.000200.01117excludedK562TARDBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RBM8A is not avaliable on ENCORE database.