| HepG2 | | 
Caption: Western blot following CRISPR against RBM8A in HepG2 whole cell lysate using RBM8A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM8A. RBM8A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGHcLV34-31 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the RBM8A specific antibody, A301-033A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM8A-specific antibody, A301-033A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Comments: The IP band isn't always stronger than the corresponding band in the input. Some antibodies that specifically recognize their target but might not strongly bind to them during the IP, i.e. some of the immunoprecipitated material could be washed away. Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against RBM8A in K562 whole cell lysate using RBM8A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM8A. RBM8A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGKcLV30-23 Status: Released Lab: Graveley Lab |
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