EIF2AK2

General Information

Gene Name       : EIF2AK2
Gene ID             : ENSG00000055332.17
Essential Gene : No

Functions

Localization

Domains (AlphaFold: EIF2AK2)


Antibody Validation

 View validation images for all EIF2AK2 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878EIF2AK2ENSG00000055332.1753.9345.5149.72ENCSR000AEF
HepG2EIF2AK2ENSG00000055332.1712.5825.5519.07ENCSR985KAT
K562EIF2AK2ENSG00000055332.1710.6013.1211.86ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq EIF2AK2-BGKcLV13-K562ENCSR628AFFEIF2AK2K562ReleasedENCSR712CQC   CRISPRKD% = 73.6,74.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
EIF2AK2ENCAB399AJN700286SA2330331BGKcLV13-53, Released, ENCODE4
EIF2AK212297S3Submitted, ENCORE2BGHcLV43-15, Submitted, ENCORE2BGKcLV42-27, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
EIF2AK2K562CRISPRBGKcLV13-51,BGKcLV13-52BGKcLV13-1,BGKcLV13-252.3,53.3NotSatisfiedENCODE4
EIF2AK2K562CRISPRENCSR628AFFENCSR712CQCEIF2AK2-BGKcLV13-53,EIF2AK2-BGKcLV13-54NT-BGKcLV13-1,NT-BGKcLV13-273.6,74.5ReleasedENCODE4
EIF2AK2K562CRISPREIF2AK2-BGKcLV42-27,EIF2AK2-BGKcLV42-28NT-BGKcLV42-1,NT-BGKcLV42-270.6,69.3SequencedENCORE2
EIF2AK2HepG2CRISPRBGHcLV14-51,BGHcLV14-52BGHcLV14-1,BGHcLV14-229.3,1.1NotSatisfiedENCODE4
EIF2AK2HepG2CRISPRBGHcLV14-53,BGHcLV14-54BGHcLV14-1,BGHcLV14-230.5,48.1NotSatisfiedENCODE4
EIF2AK2HepG2CRISPRBGHcLV20-17,BGHcLV20-18BGHcLV20-1,BGHcLV20-2NotSatisfiedENCODE4
EIF2AK2HepG2CRISPRBGHcLV20-19,BGHcLV20-20BGHcLV20-1,BGHcLV20-2NotSatisfiedENCODE4
EIF2AK2HepG2CRISPREIF2AK2-BGHcLV43-15,EIF2AK2-BGHcLV43-16NT-BGHcLV43-1,NT-BGHcLV43-281.8,87.1SequencedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2AK2Product_ID: 12297S
Lot_ID: 3
Source: Cell Signaling Technology
Target Name: EIF2AK2-human		 EIF2AK2-HEPG2-CRISPR-12297S.png<br>Caption: Western blot following CRISPR against EIF2AK2 in HepG2 whole cell lysate using EIF2AK2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK2. EIF2AK2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12297S_3_EiF2AK2.png<br>Caption: IP-WB analysis of 12297S whole cell lysate using the EiF2AK2 specific antibody, 12297S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EiF2AK2-specific antibody, 12297S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 E2AK2-K562-CRISPR-12297S.png<br>Caption: Western blot following CRISPR against EIF2AK2 in K562 whole cell lysate using EIF2AK2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK2. EIF2AK2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
EIF2AK2Product_ID: 700286
Lot_ID: SA2330331
Source: Thermo Fisher
Target Name: EIF2AK2-human		 PKR-K562-CRISPR.png<br>Caption: Western blot following CRISPR against EIF2AK2 in K562 whole cell lysate using EIF2AK2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK2. EIF2AK2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for EIF2AK2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • EIF2AK2 RNA is bound by 16 proteins.  







eCLIP assay for EIF2AK2 is not avaliable on ENCORE database.





16 proteins are bound to EIF2AK2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr23733315637333242-3'-UTR,intron-Distal3.816673.50377HepG2TAF15
chr23732998037330095-3'-UTR,intron-Distal5.3316511.15661HepG2HNRNPL
chr23733321637333276-3'-UTR,intron-Distal4.541054.01524K562FAM120A
chr23736874337368845-3'-UTR,CDS,intron-Distal,intron-Proximal3.723046.60490HepG2BCLAF1
chr23732876537328827-3'-UTR,intron-Distal3.826606.87319HepG2ILF3
chr23737486937374970-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.853715.24452HepG2PPIG
chr23732740537327489-3'-UTR,intron-Distal4.099843.76295K562IGF2BP2
chr23732863637328687-3'-UTR,intron-Distal3.823083.24590HepG2ILF3
chr23734977837349808-CDS,intron-Distal,intron-Proximal3.859825.36555HepG2BCLAF1
chr23735342937353460-CDS,intron-Distal,intron-Proximal4.916556.37937HepG2BCLAF1
chr23734969237349817-CDS,intron-Distal,intron-Proximal5.559039.86388K562PUM2
chr23736565137365682-CDS,intron-Distal,intron-Proximal4.111844.51739HepG2BCLAF1
chr23733633337336438-CDS,intron-Distal,intron-Proximal4.284404.21087HepG2GRWD1
chr23733443437334544-CDS,intron-Distal,intron-Proximal3.383477.01246HepG2BCLAF1
chr23736565137365713-CDS,intron-Distal,intron-Proximal3.499723.93645HepG2PPIG
chr23736540737365439-CDS,intron-Distal,intron-Proximal3.337624.74886HepG2BCLAF1
chr23733462537334672-CDS,intron-Distal,intron-Proximal3.944054.50744HepG2U2AF2
chr23734964737349703-CDS,intron-Distal,intron-Proximal3.876013.56019HepG2GRWD1
chr23735346037353552-CDS,intron-Distal4.301817.97334HepG2BCLAF1
chr23734964837349778-CDS,intron-Distal,intron-Proximal3.145389.29726HepG2BCLAF1
chr23734715437347249-CDS,intron-Distal3.805358.19964HepG2BCLAF1
chr23737483037374944-CDS,intron-Distal,intron-Proximal3.619485.97027HepG2GRWD1
chr23737402537374105-CDS,intron-Distal,intron-Proximal3.406385.15776HepG2BCLAF1
chr23734710137347289-CDS,intron-Distal,intron-Proximal4.419385.23727HepG2G3BP1
chr23736412537364161-CDS,intron-Distal,intron-Proximal3.567343.27569HepG2BCLAF1
chr23738385037383922-intron-Distal5.580365.58036HepG2EFTUD2
chr23738351537383593-intron-Distal4.157486.53456HepG2PCBP2
chr23738395937384006-intron-Distal3.026856.29405HepG2PRPF8
chr23738396037384033-intron-Distal5.348399.65239HepG2EFTUD2
chr23736005237360080-intron-Distal6.1772419.03931K562RPS11
chr23738321137383281-intron-Distal4.154764.24431HepG2RBFOX2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for EIF2AK2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for EIF2AK2 is not available on ENCORE database.

  • 87 RBPs can affect the levels of EIF2AK2.  








The Knockdown/knockout RNA-Seq assay for EIF2AK2 is not available on ENCORE database.





87 RBPs can affect the gene expression of EIF2AK2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
EIF2AK2ENSG00000055332.121955.935001679.752200.219502.632002.03590HepG2ACO1
EIF2AK2ENSG00000055332.121598.185902380.19260-0.574607.438106.51460HepG2BOP1
EIF2AK2ENSG00000055332.124334.005703249.572800.415402.452401.54150HepG2CCAR1
EIF2AK2ENSG00000055332.121424.197301899.23980-0.415304.075402.74640HepG2CPSF7
EIF2AK2ENSG00000055332.121614.878402011.25390-0.316704.122003.36760HepG2CELF1
EIF2AK2ENSG00000055332.121509.866102162.70500-0.5184013.7295012.29140HepG2DAZAP1
EIF2AK2ENSG00000055332.121821.311301553.602300.229302.863202.01550HepG2DDX19B
EIF2AK2ENSG00000055332.121296.989001610.91810-0.312703.322002.05360HepG2DDX1
EIF2AK2ENSG00000055332.121733.912501395.180300.313502.754501.84450HepG2DDX24
EIF2AK2ENSG00000055332.121360.782601943.74170-0.514404.703403.90560HepG2DDX3X
EIF2AK2ENSG00000055332.121183.371701669.67200-0.496703.851503.13260HepG2DDX6
EIF2AK2ENSG00000055332.121113.044901579.59830-0.505007.937206.79480HepG2DNAJC21
EIF2AK2ENSG00000055332.121143.278901565.64350-0.453608.980507.88570HepG2DNAJC2
EIF2AK2ENSG00000055332.121191.125801605.13820-0.430403.409702.75550HepG2EIF2S1
EIF2AK2ENSG00000055332.122265.220702613.08900-0.206102.159001.61550HepG2ETF1
EIF2AK2ENSG00000055332.121312.964001067.291500.298802.254901.53860HepG2FUBP3
EIF2AK2ENSG00000055332.121312.072701603.77760-0.289603.489202.40190HepG2HDGF
EIF2AK2ENSG00000055332.121478.476501773.84150-0.262801.897401.31160HepG2HNRNPC
EIF2AK2ENSG00000055332.121546.795601970.09310-0.349006.548805.55550HepG2HNRNPL
EIF2AK2ENSG00000055332.121728.798202363.98900-0.451504.244403.47950HepG2HNRNPLL
EIF2AK2ENSG00000055332.121458.425301762.11410-0.272903.455902.78590HepG2HNRNPU
EIF2AK2ENSG00000055332.121152.126401535.60170-0.414507.542505.91940HepG2KIF1C
EIF2AK2ENSG00000055332.121800.274302144.52770-0.252402.530601.80230HepG2NOL12
EIF2AK2ENSG00000055332.122600.641502096.064900.311102.543201.82460HepG2NSUN2
EIF2AK2ENSG00000055332.121317.399901571.66530-0.254602.440701.74390HepG2PPIL4
EIF2AK2ENSG00000055332.122320.906201761.207300.398004.953403.94970HepG2PRPF4
EIF2AK2ENSG00000055332.121360.994401091.926400.317702.168901.45100HepG2PRPF8
EIF2AK2ENSG00000055332.121870.806102171.49420-0.215001.857401.30220HepG2PUM2
EIF2AK2ENSG00000055332.121312.308901710.95960-0.382706.362305.49670HepG2PUS1
EIF2AK2ENSG00000055332.123066.680401897.399400.692604.499003.61130HepG2RAVER1
EIF2AK2ENSG00000055332.122123.048202632.12760-0.310103.288502.36170HepG2RBM15
EIF2AK2ENSG00000055332.121983.983601609.140200.302002.817102.13690HepG2RBM25
EIF2AK2ENSG00000055332.121948.986001697.041900.199602.132201.58200HepG2RECQL
EIF2AK2ENSG00000055332.122212.889901704.398700.376606.327505.49390HepG2SF3B4
EIF2AK2ENSG00000055332.121449.705301830.00320-0.336105.966805.07800HepG2SMN1
EIF2AK2ENSG00000055332.122539.688201779.078600.5134012.2047011.36290HepG2SRSF1
EIF2AK2ENSG00000055332.121779.204902021.17670-0.184001.889001.35840HepG2SRSF5
EIF2AK2ENSG00000055332.121728.998301447.422900.256303.246602.65830HepG2SRSF9
EIF2AK2ENSG00000055332.122093.069801599.258800.388105.542104.79570HepG2TAF15
EIF2AK2ENSG00000055332.121447.659701712.23100-0.242202.040701.59790HepG2TIAL1
EIF2AK2ENSG00000055332.121281.859301727.52860-0.430507.366206.52220HepG2TRIM56
EIF2AK2ENSG00000055332.122913.538301991.897700.548506.909405.92820HepG2U2AF1
EIF2AK2ENSG00000055332.122102.020002546.08380-0.276505.046104.36210HepG2ZNF622
EIF2AK2ENSG00000055332.122634.823801895.228600.475203.211501.80500HepG2ZRANB2
EIF2AK2ENSG00000055332.123606.963102853.740600.337802.950701.68100K562ABCF1
EIF2AK2ENSG00000055332.121550.237501881.07050-0.279103.513502.88650K562AGGF1
EIF2AK2ENSG00000055332.121400.560601957.31370-0.482905.510104.57180K562AKAP8L
EIF2AK2ENSG00000055332.122361.285401836.956500.362202.914002.31360K562AQR
EIF2AK2ENSG00000055332.122000.467201429.008900.485204.022502.96830K562BOP1
EIF2AK2ENSG00000055332.12867.27880623.770600.475403.832902.55020K562CCAR1
EIF2AK2ENSG00000055332.123824.342003229.038800.244002.503701.57660K562CELF1
EIF2AK2ENSG00000055332.123681.479102613.521400.4942011.9183010.66910K562DDX47
EIF2AK2ENSG00000055332.12965.23640698.317000.466903.637302.08180K562DHX30
EIF2AK2ENSG00000055332.12896.80910670.761800.418903.162801.72380K562DNAJC21
EIF2AK2ENSG00000055332.121492.38730634.479901.2339012.4602010.35560K562EIF3A
EIF2AK2ENSG00000055332.121681.984801196.458600.491303.778602.55380K562EIF3D
EIF2AK2ENSG00000055332.12878.521601103.75810-0.329303.056101.82470K562EIF4B
EIF2AK2ENSG00000055332.123401.844102626.188000.373206.495705.07850K562EIF4G2
EIF2AK2ENSG00000055332.121522.575302051.22930-0.430004.407503.32050K562FIP1L1
EIF2AK2ENSG00000055332.121609.743601046.264600.621506.486804.62670K562FKBP4
EIF2AK2ENSG00000055332.122097.563801546.152500.439902.991202.10040K562FMR1
EIF2AK2ENSG00000055332.121900.977701310.642600.536403.022502.06260K562FTO
EIF2AK2ENSG00000055332.121931.429701394.455900.469903.190402.42270K562G3BP2
EIF2AK2ENSG00000055332.121558.285202023.99070-0.377202.540501.81360K562GRSF1
EIF2AK2ENSG00000055332.121929.661001285.903100.585506.429404.83460K562HNRNPA1
EIF2AK2ENSG00000055332.122149.436701205.415200.8343016.3132014.94280K562HNRNPC
EIF2AK2ENSG00000055332.12859.694501687.09050-0.9726012.4877010.14290K562HNRNPM
EIF2AK2ENSG00000055332.121990.296201423.901900.483005.400504.14030K562HNRNPU
EIF2AK2ENSG00000055332.12883.84400651.702700.439503.014701.45250K562ILF3
EIF2AK2ENSG00000055332.12951.82450629.787300.595705.387003.31530K562KHDRBS1
EIF2AK2ENSG00000055332.121582.655301988.40360-0.329302.904001.79580K562MAK16
EIF2AK2ENSG00000055332.121803.930202327.07470-0.367404.089502.95020K562MBNL1
EIF2AK2ENSG00000055332.122340.891601705.293900.456905.020303.95700K562MTPAP
EIF2AK2ENSG00000055332.122347.129401894.965500.308602.219801.54420K562NSUN2
EIF2AK2ENSG00000055332.123538.305002413.484100.551803.344401.96640K562PABPC1
EIF2AK2ENSG00000055332.12903.433901176.80370-0.381402.943001.46680K562PHF6
EIF2AK2ENSG00000055332.121371.670902328.44300-0.7634011.788509.74390K562PPIG
EIF2AK2ENSG00000055332.12701.785601080.76080-0.622907.087005.52310K562PSIP1
EIF2AK2ENSG00000055332.121025.301301468.03590-0.517803.568202.82520K562PTBP1
EIF2AK2ENSG00000055332.123697.425502865.376600.367703.571402.64670K562PUF60
EIF2AK2ENSG00000055332.123925.950803047.726100.365204.908203.45520K562PUM1
EIF2AK2ENSG00000055332.121579.207301941.29950-0.297802.376701.86490K562PUS1
EIF2AK2ENSG00000055332.121456.619401891.35360-0.376804.799604.03890K562RBM15
EIF2AK2ENSG00000055332.121844.116901107.656600.735308.192506.77190K562RPS19
EIF2AK2ENSG00000055332.123617.499302240.980000.690805.214902.95490K562RPS3A
EIF2AK2ENSG00000055332.122274.819301379.870500.721104.191302.97440K562SF3B1
EIF2AK2ENSG00000055332.12971.88850687.009500.500404.329302.71670K562SLBP
EIF2AK2ENSG00000055332.123011.240502500.073300.268302.957801.39990K562SUCLG1
EIF2AK2ENSG00000055332.121013.452001378.26780-0.443602.706001.62840K562TRA2A
EIF2AK2ENSG00000055332.121957.946001396.322600.487606.925405.66240K562U2AF2
EIF2AK2ENSG00000055332.121465.903901114.317000.395503.150302.06080K562UCHL5
EIF2AK2ENSG00000055332.122578.376101914.313500.429503.511702.15160K562UTP3
EIF2AK2ENSG00000055332.122281.557101719.284900.408102.710301.84260K562WDR3
EIF2AK2ENSG00000055332.122480.754501787.651800.472605.282004.14950K562WDR43
EIF2AK2ENSG00000055332.122647.491102176.594300.282502.567901.32940K562WRN
EIF2AK2ENSG00000055332.122623.656502069.323900.342303.258102.31270K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for EIF2AK2 is not available on ENCORE database.





4 RBPs can affect the splicing of EIF2AK2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEEIF2AK2ENSG00000055332.12chr2-373418733734200237336336373364383734710137347282-0.100000.000980.01391excludedHepG2U2AF2
A3SSEIF2AK2ENSG00000055332.12chr2-3737599937376400373759993737616637384050373841900.136000.007580.04158longerK562AQR
A3SSEIF2AK2ENSG00000055332.12chr2-3737483037375016373748303737496537375999373760580.080000.005500.03146longerK562AQR
MXEEIF2AK2ENSG00000055332.12chr2-37349648373498073735343137353554373471013734728237362626373626890.108000.005590.078481includedHepG2FTO
MXEEIF2AK2ENSG00000055332.12chr2-3734964837349807373534313735355437347101373472823736262637362689-0.148000.011260.069702includedK562AQR
MXEEIF2AK2ENSG00000055332.12chr2-37349648373498073735343137353554373471013734728237362626373626890.082000.004890.029981includedHepG2U2AF2
MXEEIF2AK2ENSG00000055332.12chr2-37349648373498073735343137353554373471013734728237362626373626890.102000.009890.096101includedK562HNRNPUL1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for EIF2AK2 is not avaliable on ENCORE database.