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EIF2AK2 RNA-Seq in HepG2



EIF2AK2      CRISPR in HepG2      Control:

General Information
RBPEIF2AK2
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF2AK2-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF2AK2-BGHcLV43-15
Rep2EIF2AK2-BGHcLV43-16
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR0.0
Rep2_qPCR0.0
Rep1_WB81.8
Rep2_WB87.1
Antibody Cat#12297S
Antibody Lot#lot # 3
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1574




Experiment Information (Status: Sequenced)
BGHcLV43-15BGHcLV43-16
idx13831384
TRCN#_or_BGC#BGC#0001131BGC#0001131
shRNA_or_gRNA_sequenceAATACATACCGTCAGAAGCAAATACATACCGTCAGAAGCA
PAMGGGGGG
NameE2AK2_84E2AK2_84
Sample_IDBGHcLV43-15BGHcLV43-16
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameEIF2AK2EIF2AK2
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-FTTCACAGACCTACGGGATGG
RT-qPCR_primer-RGGTCGATCCTCAGGTTTCTTT
protein_conc30173241
WB_result81.887.1
Ave_WB84.4
WB_DONE_date6/13/24
MW74kd
IP
antibody_Cat#12297Slot # 3
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75887589




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2AK2Product_ID: 12297S
Lot_ID: 3
Source: Cell Signaling Technology
Target Name: EIF2AK2-human		 EIF2AK2-HEPG2-CRISPR-12297S.png<br>Caption: Western blot following CRISPR against EIF2AK2 in HepG2 whole cell lysate using EIF2AK2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK2. EIF2AK2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12297S_3_EiF2AK2.png<br>Caption: IP-WB analysis of 12297S whole cell lysate using the EiF2AK2 specific antibody, 12297S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EiF2AK2-specific antibody, 12297S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 E2AK2-K562-CRISPR-12297S.png<br>Caption: Western blot following CRISPR against EIF2AK2 in K562 whole cell lysate using EIF2AK2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK2. EIF2AK2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-15BGHcLV43-16
Sample_IDBGHcLV43-15BGHcLV43-16
Sample Name
Sample NameEIF2AK2-BGHcLV43-15EIF2AK2-BGHcLV43-16
RBPEIF2AK2EIF2AK2
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1323813239




Library-Prep Information
BGHcLV43-15BGHcLV43-16
Sample #1112
Sample NameEIF2AK2-BGHcLV43-15EIF2AK2-BGHcLV43-16
Sample_Name_Alias
Index Well PositionC07D07
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp301310
Peak_Molarity94.8088.40
libSampleQC_DNA_WellDNA_Library_set74/set1/C2.pngDNA_Library_set74/set1/D2.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/G7.pngRNA_Library_set74/set1/H7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-15BGHcLV43-16
RBPEIF2AK2EIF2AK2
Batch_IDBGHcLV43BGHcLV43
WB_result81.80087.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43704371




Sequencing Information
BGHcLV43-15BGHcLV43-16
Sample_IDBGHcLV43-15BGHcLV43-16
Sample NameEIF2AK2-BGHcLV43-15EIF2AK2-BGHcLV43-16
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads42,108,57445,121,344
total_aligned_reads38,095,77241,787,418
unique_aligned_reads35,834,43139,324,245
percent_uniqueAligned0.851000.87152
correlation_replicates0.9973770.997377
spikein_reads32,25811,068
percent_spikeins0.000770.00025
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32373238




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database