non-target      CRISPR in HepG2      Control: NT-BGHcLV43-1,NT-BGHcLV43-2

General Information

List KD experiments controlled by BGHcLV43-1 and BGHcLV43-2


NT-BGHcLV43-1,NT-BGHcLV43-2:
  • BRD4-BGHcLV43-HepG2
  • CNOT3-BGHcLV43-HepG2
  • CRKL-BGHcLV43-HepG2
  • DDX41-BGHcLV43-HepG2
  • EIF2AK2-BGHcLV43-HepG2
  • EIF1-BGHcLV43-HepG2
  • EIF4H-BGHcLV43-HepG2
  • HEXIM1-BGHcLV43-HepG2
  • KARS-BGHcLV43-HepG2
  • LARP1-BGHcLV43-HepG2
  • NAA10-BGHcLV43-HepG2
  • NOP2-BGHcLV43-HepG2
  • PABPC1-BGHcLV43-HepG2
  • PARK7-BGHcLV43-HepG2
  • PKM-BGHcLV43-HepG2
  • PRKRA-BGHcLV43-HepG2
  • PSMD4-BGHcLV43-HepG2
  • RBBP5-BGHcLV43-HepG2
  • SEC24D-BGHcLV43-HepG2
  • SSU72-BGHcLV43-HepG2
  • TFAM-BGHcLV43-HepG2
  • UBA2-BGHcLV43-HepG2
  • USP15-BGHcLV43-HepG2
  • USP39-BGHcLV43-HepG2
  • VASP-BGHcLV43-HepG2




    • Experiment Information (Status: Submitting)
    BGHcLV43-1BGHcLV43-2
    idx13691370
    TRCN#_or_BGC#
    shRNA_or_gRNA_sequence
    PAM
    Namenonnon
    Sample_IDBGHcLV43-1BGHcLV43-2
    transduction_Date6/6/246/6/24
    daysD6D6
    RBP_namenon-targetnon-target
    qPCR_result
    Ave_qPCR
    RT-qPCR_primer-F
    RT-qPCR_primer-R
    protein_conc03098
    WB_result
    Ave_WB
    WB_DONE_date
    MW
    IP
    antibody_Cat#
    Antibody DCC ID
    submitted_to_DCC_date
    Rep1_TPM00
    Rep2_TPM00
    ActionReadyReady
    Library_start_date
    repeat_library
    Note
    ID96149615




    Western Blot
    Western Blot info was not avaliable




    Experiment Status
    BGHcLV43-1BGHcLV43-2
    Sample_IDBGHcLV43-1BGHcLV43-2
    Sample Name
    Sample NameNT-BGHcLV43-1NT-BGHcLV43-2
    RBPnon-targetnon-target
    Cell_LineHepG2HepG2
    Exp UID
    StatusSubmittingSubmitting
    Status_date2025-02-112025-02-11
    ProjectENCORE2ENCORE2
    Note
    ID1322813229




    Library-Prep Information
    BGHcLV43-1BGHcLV43-2
    Sample #12
    Sample NameNT-BGHcLV43-1NT-BGHcLV43-2
    Sample_Name_Alias
    Index Well PositionA06B06
    Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
    LibPrep_date2024-07-222024-07-22
    Lib_IDLib-240722Lib-240722
    Tecan_Location
    Tecan
    Tecan_date
    Size_bp292304
    Peak_Molarity103.0091.70
    libSampleQC_DNA_WellDNA_Library_set74/set1/A1.pngDNA_Library_set74/set1/B1.png
    RIN10.010.0
    libSample_RNA_WellRNA_Library_set74/set1/E6.pngRNA_Library_set74/set1/F6.png
    SampleQC_methodTapeStation_2022TapeStation_2022
    SampleQC_date2024-07-232024-07-23
    Sample_IDBGHcLV43-1BGHcLV43-2
    RBPNTNT
    Batch_IDBGHcLV43BGHcLV43
    WB_result0.0000.000
    Library DescriptionTruSeq mRNATruSeq mRNA
    Repeat_Library_Suffix
    Lib_StatusSendToSequenceSendToSequence
    ProjectENCORE2ENCORE2
    ID43604361




    Sequencing Information
    BGHcLV43-1BGHcLV43-2
    Sample_IDBGHcLV43-1BGHcLV43-2
    Sample NameNT-BGHcLV43-1NT-BGHcLV43-2
    Pool IDPool-240723Pool-240723
    LocalServer_folderset74set74
    total_reads32,564,93630,636,500
    total_aligned_reads27,791,47228,037,876
    unique_aligned_reads26,088,26726,369,519
    percent_uniqueAligned0.801120.86072
    correlation_replicates0.9956770.995677
    spikein_reads10,93214,410
    percent_spikeins0.000340.00047
    original_ReadLength101101
    QC_StatusSubmittingSubmitting
    ID32273228




    Data Submission Information
    DCC submission information was not avaliable




    File Information
    file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
    NT-BGHcLV43-1_S91_L003_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set74/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3227NovaSeq6000100paired-ended1ENCORE230544
    NT-BGHcLV43-1_S91_L003_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set74/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3227NovaSeq6000100paired-ended2ENCORE230596
    NT-BGHcLV43-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3227GRCh38V40NT-BGHcLV43-1_S91_L003_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV43-1_S91_L003_R2_001.filtered.trimmed.paired.fastq.gzENCORE230648
    NT-BGHcLV43-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3227GRCh38V40NT-BGHcLV43-1_Aligned.sortedByCoord.out.bamENCORE230700
    NT-BGHcLV43-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3227GRCh38V40NT-BGHcLV43-1_Aligned.sortedByCoord.out.bamENCORE230752
    NT-BGHcLV43-1_Signal.Unique.strand-.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3227GRCh38V40NT-BGHcLV43-1_Aligned.sortedByCoord.out.bamENCORE230804
    NT-BGHcLV43-1_Signal.Unique.strand+.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3227GRCh38V40NT-BGHcLV43-1_Aligned.sortedByCoord.out.bamENCORE230856
    NT-BGHcLV43-1_quant.sfENCODE_DATA/set74/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3227GRCh38V40NT-BGHcLV43-1_S91_L003_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV43-1_S91_L003_R2_001.filtered.trimmed.paired.fastq.gzENCORE230908
    NT-BGHcLV43-2_S92_L003_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set74/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3228NovaSeq6000100paired-ended1ENCORE230545
    NT-BGHcLV43-2_S92_L003_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set74/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3228NovaSeq6000100paired-ended2ENCORE230597
    NT-BGHcLV43-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3228GRCh38V40NT-BGHcLV43-2_S92_L003_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV43-2_S92_L003_R2_001.filtered.trimmed.paired.fastq.gzENCORE230649
    NT-BGHcLV43-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3228GRCh38V40NT-BGHcLV43-2_Aligned.sortedByCoord.out.bamENCORE230701
    NT-BGHcLV43-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3228GRCh38V40NT-BGHcLV43-2_Aligned.sortedByCoord.out.bamENCORE230753
    NT-BGHcLV43-2_Signal.Unique.strand-.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3228GRCh38V40NT-BGHcLV43-2_Aligned.sortedByCoord.out.bamENCORE230805
    NT-BGHcLV43-2_Signal.Unique.strand+.bwENCODE_DATA/set74/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3228GRCh38V40NT-BGHcLV43-2_Aligned.sortedByCoord.out.bamENCORE230857
    NT-BGHcLV43-2_quant.sfENCODE_DATA/set74/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3228GRCh38V40NT-BGHcLV43-2_S92_L003_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV43-2_S92_L003_R2_001.filtered.trimmed.paired.fastq.gzENCORE230909