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USP15 RNA-Seq in HepG2



USP15      CRISPR in HepG2      Control:

General Information
RBPUSP15
Cell_LineHepG2
MethodCRISPR
Exp_NameUSP15-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1USP15-BGHcLV43-59
Rep2USP15-BGHcLV43-60
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR0.0
Rep2_qPCR0.0
Rep1_WB84.3
Rep2_WB88.4
Antibody Cat#66310S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1592




Experiment Information (Status: Sequenced)
BGHcLV43-59BGHcLV43-60
idx14271428
TRCN#_or_BGC#BGC#0001202BGC#0001202
shRNA_or_gRNA_sequenceTGAACCAGCGACTATCGACTTGAACCAGCGACTATCGACT
PAMAGGAGG
NameUSP15_96USP15_96
Sample_IDBGHcLV43-59BGHcLV43-60
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameUSP15USP15
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-Ftgtgtatcctggacccattg
RT-qPCR_primer-Raagtgactgggcatcaccat
protein_conc43234163
WB_result84.388.4
Ave_WB86.3
WB_DONE_date6/21/24
MW120kd
IP
antibody_Cat#66310Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76347635




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
USP15Product_ID: 66310S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: USP15-human		 USP15-HEPG2-CRISPR-66310S.png<br>Caption: Western blot following CRISPR against USP15 in HepG2 whole cell lysate using USP15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against USP15. USP15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_66310S_1_USP15.png<br>Caption: IP-WB analysis of 66310S whole cell lysate using the USP15 specific antibody, 66310S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the USP15-specific antibody, 66310S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 USP15-K562-CRISPR-66310S.png<br>Caption: Western blot following CRISPR against USP15 in K562 whole cell lysate using USP15 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against USP15. USP15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-59BGHcLV43-60
Sample_IDBGHcLV43-59BGHcLV43-60
Sample Name
Sample NameUSP15-BGHcLV43-59USP15-BGHcLV43-60
RBPUSP15USP15
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1327413275




Library-Prep Information
BGHcLV43-59BGHcLV43-60
Sample #4748
Sample NameUSP15-BGHcLV43-59USP15-BGHcLV43-60
Sample_Name_Alias
Index Well PositionG11H11
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp293288
Peak_Molarity65.5069.40
libSampleQC_DNA_WellDNA_Library_set74/set1/G6.pngDNA_Library_set74/set1/H6.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/C12.pngRNA_Library_set74/set1/D12.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-59BGHcLV43-60
RBPUSP15USP15
Batch_IDBGHcLV43BGHcLV43
WB_result84.30088.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID44064407




Sequencing Information
BGHcLV43-59BGHcLV43-60
Sample_IDBGHcLV43-59BGHcLV43-60
Sample NameUSP15-BGHcLV43-59USP15-BGHcLV43-60
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads51,476,36058,717,015
total_aligned_reads47,962,55854,687,315
unique_aligned_reads44,979,27551,504,440
percent_uniqueAligned0.873790.87716
correlation_replicates0.9911400.991140
spikein_reads27,14844,638
percent_spikeins0.000530.00076
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32733274




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database