Notice: Undefined offset: 1 in /website/encore_alpha/gene/index.php on line 599
CRKL RNA-Seq in HepG2



CRKL      CRISPR in HepG2      Control:

General Information
RBPCRKL
Cell_LineHepG2
MethodCRISPR
Exp_NameCRKL-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1CRKL-BGHcLV43-11
Rep2CRKL-BGHcLV43-12
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR16.7
Rep2_qPCR36.7
Rep1_WB87.5
Rep2_WB89.4
Antibody Cat#38710S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1572




Experiment Information (Status: Sequenced)
BGHcLV43-11BGHcLV43-12
idx13791380
TRCN#_or_BGC#BGC#0001123BGC#0001123
shRNA_or_gRNA_sequenceGTCCGAGGAGTCGAACCTGGGTCCGAGGAGTCGAACCTGG
PAMCGGCGG
NameCRKL_91CRKL_91
Sample_IDBGHcLV43-11BGHcLV43-12
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameCRKLCRKL
qPCR_result16.736.7
Ave_qPCR26.7
RT-qPCR_primer-Ftaagatcggggaccaggaat
RT-qPCR_primer-Rggttgggtgctgagacagat
protein_conc30993339
WB_result87.589.4
Ave_WB88.5
WB_DONE_date6/13/24
MW39KDTUBULIN
IP
antibody_Cat#38710Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75827583




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CRKLProduct_ID: 38710S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: CRKL-human		 CRKL-HEPG2-CRISPR-38710S.png<br>Caption: Western blot following CRISPR against CRKL in HepG2 whole cell lysate using CRKL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CRKL. CRKL protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_38710S_1_CRKL.png<br>Caption: IP-WB analysis of 38710S whole cell lysate using the CRKL specific antibody, 38710S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CRKL-specific antibody, 38710S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CRKL-K562-CRISPR-38710S.png<br>Caption: Western blot following CRISPR against CRKL in K562 whole cell lysate using CRKL specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CRKL. CRKL protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-11BGHcLV43-12
Sample_IDBGHcLV43-11BGHcLV43-12
Sample Name
Sample NameCRKL-BGHcLV43-11CRKL-BGHcLV43-12
RBPCRKLCRKL
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1323413235




Library-Prep Information
BGHcLV43-11BGHcLV43-12
Sample #78
Sample NameCRKL-BGHcLV43-11CRKL-BGHcLV43-12
Sample_Name_Alias
Index Well PositionG06H06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp288293
Peak_Molarity72.3068.60
libSampleQC_DNA_WellDNA_Library_set74/set1/G1.pngDNA_Library_set74/set1/H1.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/C7.pngRNA_Library_set74/set1/D7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-11BGHcLV43-12
RBPCRKLCRKL
Batch_IDBGHcLV43BGHcLV43
WB_result87.50089.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43664367




Sequencing Information
BGHcLV43-11BGHcLV43-12
Sample_IDBGHcLV43-11BGHcLV43-12
Sample NameCRKL-BGHcLV43-11CRKL-BGHcLV43-12
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads43,322,71140,952,934
total_aligned_reads38,769,98337,841,262
unique_aligned_reads36,419,23735,499,916
percent_uniqueAligned0.840650.86685
correlation_replicates0.9961960.996196
spikein_reads26,60813,592
percent_spikeins0.000610.00033
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32333234




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database