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HEXIM1 RNA-Seq in HepG2



HEXIM1      CRISPR in HepG2      Control:

General Information
RBPHEXIM1
Cell_LineHepG2
MethodCRISPR
Exp_NameHEXIM1-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1HEXIM1-BGHcLV43-23
Rep2HEXIM1-BGHcLV43-24
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR17.3
Rep2_qPCR37.1
Rep1_WB96.8
Rep2_WB95.7
Antibody Cat#12604S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1577




Experiment Information (Status: Sequenced)
BGHcLV43-23BGHcLV43-24
idx13911392
TRCN#_or_BGC#BGC#0001144BGC#0001144
shRNA_or_gRNA_sequenceAGAAGTGGAACCGACGCCCGAGAAGTGGAACCGACGCCCG
PAMAGGAGG
NameHEXIM1_95HEXIM1_95
Sample_IDBGHcLV43-23BGHcLV43-24
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameHEXIM1HEXIM1
qPCR_result17.337.1
Ave_qPCR27.2
RT-qPCR_primer-Ftgtccaggaagagctgaacc
RT-qPCR_primer-Rcgattgccacctactgtcct
protein_conc31913179
WB_result96.895.7
Ave_WB96.2
WB_DONE_date6/13/24
MW60kd
IP
antibody_Cat#12604Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75967597




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
HEXIM1Product_ID: 12604S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: HEXIM1-human		 HEXIM1-HEPG2-CRISPR-12604S.png<br>Caption: Western blot following CRISPR against HEXIM1 in HepG2 whole cell lysate using HEXIM1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HEXIM1. HEXIM1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12604S_1_HEXIM1.png<br>Caption: IP-WB analysis of 12604S whole cell lysate using the HEXIM1 specific antibody, 12604S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HEXIM1-specific antibody, 12604S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 HEXIM1-K562-CRISPR-12604S.png<br>Caption: Western blot following CRISPR against HEXIM1 in K562 whole cell lysate using HEXIM1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HEXIM1. HEXIM1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-23BGHcLV43-24
Sample_IDBGHcLV43-23BGHcLV43-24
Sample Name
Sample NameHEXIM1-BGHcLV43-23HEXIM1-BGHcLV43-24
RBPHEXIM1HEXIM1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1324413245




Library-Prep Information
BGHcLV43-23BGHcLV43-24
Sample #1718
Sample NameHEXIM1-BGHcLV43-23HEXIM1-BGHcLV43-24
Sample_Name_Alias
Index Well PositionA08B08
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp300305
Peak_Molarity108.00117.00
libSampleQC_DNA_WellDNA_Library_set74/set1/A3.pngDNA_Library_set74/set1/B3.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/E8.pngRNA_Library_set74/set1/F8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-23BGHcLV43-24
RBPHEXIM1HEXIM1
Batch_IDBGHcLV43BGHcLV43
WB_result96.80095.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43764377




Sequencing Information
BGHcLV43-23BGHcLV43-24
Sample_IDBGHcLV43-23BGHcLV43-24
Sample NameHEXIM1-BGHcLV43-23HEXIM1-BGHcLV43-24
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads31,437,12341,809,600
total_aligned_reads28,220,52435,201,318
unique_aligned_reads26,558,44033,142,285
percent_uniqueAligned0.844810.79270
correlation_replicates0.9971560.997156
spikein_reads12,67425,696
percent_spikeins0.000400.00061
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32433244




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database