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EIF1 RNA-Seq in HepG2



EIF1      CRISPR in HepG2      Control:

General Information
RBPEIF1
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF1-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF1-BGHcLV43-17
Rep2EIF1-BGHcLV43-18
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR45.9
Rep2_qPCR40.1
Rep1_WB60.5
Rep2_WB75.6716
Antibody Cat#12496S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1575




Experiment Information (Status: Sequenced)
BGHcLV43-17BGHcLV43-18
idx13851386
TRCN#_or_BGC#BGC#0001134BGC#0001134
shRNA_or_gRNA_sequenceCCACTCTTTCGGTAAGCTATCCACTCTTTCGGTAAGCTAT
PAMGGGGGG
NameEIF1_94EIF1_94
Sample_IDBGHcLV43-17BGHcLV43-18
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameEIF1EIF1
qPCR_result45.940.1
Ave_qPCR43.0
RT-qPCR_primer-Fcagagaaacggcaggaagac
RT-qPCR_primer-Rtctccatattccggatgctc
protein_conc29153113
WB_result60.575.6716
Ave_WB68.1
WB_DONE_date6/13/24,6/21/24
MW13kd
IP
antibody_Cat#12496Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75907591




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF1Product_ID: 12496S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF1-human		 EIF1-HEPG2-CRISPR-12496S.png<br>Caption: Western blot following CRISPR against EIF1 in HepG2 whole cell lysate using EIF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF1. EIF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12496S_1_EIF1.png<br>Caption: IP-WB analysis of 12496S whole cell lysate using the EIF1 specific antibody, 12496S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF1-specific antibody, 12496S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF1-K562-CRISPR-12496S.png<br>Caption: Western blot following CRISPR against EIF1 in K562 whole cell lysate using EIF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF1. EIF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-17BGHcLV43-18
Sample_IDBGHcLV43-17BGHcLV43-18
Sample Name
Sample NameEIF1-BGHcLV43-17EIF1-BGHcLV43-18
RBPEIF1EIF1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1324013241




Library-Prep Information
BGHcLV43-17BGHcLV43-18
Sample #1314
Sample NameEIF1-BGHcLV43-17EIF1-BGHcLV43-18
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp301300
Peak_Molarity89.6086.80
libSampleQC_DNA_WellDNA_Library_set74/set1/E2.pngDNA_Library_set74/set1/F2.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/A8.pngRNA_Library_set74/set1/B8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-17BGHcLV43-18
RBPEIF1EIF1
Batch_IDBGHcLV43BGHcLV43
WB_result60.50075.672
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43724373




Sequencing Information
BGHcLV43-17BGHcLV43-18
Sample_IDBGHcLV43-17BGHcLV43-18
Sample NameEIF1-BGHcLV43-17EIF1-BGHcLV43-18
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads41,881,70033,289,508
total_aligned_reads38,464,26431,129,192
unique_aligned_reads36,176,26529,278,489
percent_uniqueAligned0.863770.87951
correlation_replicates0.9998190.999819
spikein_reads21,91414,600
percent_spikeins0.000520.00044
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32393240




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database