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LARP1 RNA-Seq in HepG2



LARP1      CRISPR in HepG2      Control:

General Information
RBPLARP1
Cell_LineHepG2
MethodCRISPR
Exp_NameLARP1-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1LARP1-BGHcLV43-27
Rep2LARP1-BGHcLV43-28
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR37.0
Rep2_qPCR29.7
Rep1_WB88.1
Rep2_WB88.0
Antibody Cat#70180S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1579




Experiment Information (Status: Sequenced)
BGHcLV43-27BGHcLV43-28
idx13951396
TRCN#_or_BGC#BGC#0001153BGC#0001153
shRNA_or_gRNA_sequenceGTCTGCCACCTACGTGCCCGGTCTGCCACCTACGTGCCCG
PAMTGGTGG
NameLARP1_92LARP1_92
Sample_IDBGHcLV43-27BGHcLV43-28
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameLARP1LARP1
qPCR_result37.029.7
Ave_qPCR33.4
RT-qPCR_primer-FAACTGCAAGAATACCTCGGC
RT-qPCR_primer-RCACTGAGTGTCGCTTGTGGT
protein_conc34693556
WB_result88.188.0
Ave_WB88.1
WB_DONE_date6/21/24
MW150kd
IP
antibody_Cat#70180Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76007601




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
LARP1Product_ID: 70180S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: LARP1-human		 LARP1-HEPG2-CRISPR-70180S.png<br>Caption: Western blot following CRISPR against LARP1 in HepG2 whole cell lysate using LARP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against LARP1. LARP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_70180S_1_LARP1.png<br>Caption: IP-WB analysis of 70180S whole cell lysate using the LARP1 specific antibody, 70180S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the LARP1-specific antibody, 70180S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 LARP1-K562-CRISPR-70180S.png<br>Caption: Western blot following CRISPR against LARP1 in K562 whole cell lysate using LARP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against LARP1. LARP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-27BGHcLV43-28
Sample_IDBGHcLV43-27BGHcLV43-28
Sample Name
Sample NameLARP1-BGHcLV43-27LARP1-BGHcLV43-28
RBPLARP1LARP1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1324813249




Library-Prep Information
BGHcLV43-27BGHcLV43-28
Sample #2122
Sample NameLARP1-BGHcLV43-27LARP1-BGHcLV43-28
Sample_Name_Alias
Index Well PositionE08F08
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp293291
Peak_Molarity87.5098.70
libSampleQC_DNA_WellDNA_Library_set74/set1/E3.pngDNA_Library_set74/set1/F3.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/A9.pngRNA_Library_set74/set1/B9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-27BGHcLV43-28
RBPLARP1LARP1
Batch_IDBGHcLV43BGHcLV43
WB_result88.10088.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43804381




Sequencing Information
BGHcLV43-27BGHcLV43-28
Sample_IDBGHcLV43-27BGHcLV43-28
Sample NameLARP1-BGHcLV43-27LARP1-BGHcLV43-28
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads44,328,62349,768,854
total_aligned_reads39,113,61345,505,707
unique_aligned_reads36,891,69242,909,935
percent_uniqueAligned0.832230.86218
correlation_replicates0.9981710.998171
spikein_reads22,47631,756
percent_spikeins0.000510.00064
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32473248




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database