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NOP2 RNA-Seq in HepG2



NOP2      CRISPR in HepG2      Control:

General Information
RBPNOP2
Cell_LineHepG2
MethodCRISPR
Exp_NameNOP2-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1NOP2-BGHcLV43-35
Rep2NOP2-BGHcLV43-36
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR47.1
Rep2_qPCR33.0
Rep1_WB94.7
Rep2_WB82.8
Antibody Cat#90709S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1581




Experiment Information (Status: Sequenced)
BGHcLV43-35BGHcLV43-36
idx14031404
TRCN#_or_BGC#BGC#0001162BGC#0001162
shRNA_or_gRNA_sequenceGCGCAAGTTGGACCCTACGAGCGCAAGTTGGACCCTACGA
PAMAGGAGG
NameNOP2_98NOP2_98
Sample_IDBGHcLV43-35BGHcLV43-36
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameNOP2NOP2
qPCR_result47.133.0
Ave_qPCR40.1
RT-qPCR_primer-Fgagcggctcaagagtgttgt
RT-qPCR_primer-Rcatccagcagtactcggtca
protein_conc36123681
WB_result94.782.8
Ave_WB88.7
WB_DONE_date6/17/24
MW110kd
IP
antibody_Cat#90709Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76087609




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NOP2Product_ID: 90709S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NOP2-human		 NOP2-HEPG2-CRISPR-90709S.png<br>Caption: Western blot following CRISPR against NOP2 in HepG2 whole cell lysate using NOP2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NOP2. NOP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_90709S_1_NOP2.png<br>Caption: IP-WB analysis of 90709S whole cell lysate using the NOP2 specific antibody, 90709S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NOP2-specific antibody, 90709S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NOP2-K562-CRISPR-90709S.png<br>Caption: Western blot following CRISPR against NOP2 in K562 whole cell lysate using NOP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NOP2. NOP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-35BGHcLV43-36
Sample_IDBGHcLV43-35BGHcLV43-36
Sample Name
Sample NameNOP2-BGHcLV43-35NOP2-BGHcLV43-36
RBPNOP2NOP2
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1325213253




Library-Prep Information
BGHcLV43-35BGHcLV43-36
Sample #2526
Sample NameNOP2-BGHcLV43-35NOP2-BGHcLV43-36
Sample_Name_Alias
Index Well PositionA09B09
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp288295
Peak_Molarity112.00103.00
libSampleQC_DNA_WellDNA_Library_set74/set1/A4.pngDNA_Library_set74/set1/B4.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/E9.pngRNA_Library_set74/set1/F9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-35BGHcLV43-36
RBPNOP2NOP2
Batch_IDBGHcLV43BGHcLV43
WB_result94.70082.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43844385




Sequencing Information
BGHcLV43-35BGHcLV43-36
Sample_IDBGHcLV43-35BGHcLV43-36
Sample NameNOP2-BGHcLV43-35NOP2-BGHcLV43-36
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads33,559,97131,465,467
total_aligned_reads26,104,94724,926,980
unique_aligned_reads24,506,89123,426,464
percent_uniqueAligned0.730240.74451
correlation_replicates0.9990690.999069
spikein_reads23,69411,160
percent_spikeins0.000710.00035
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32513252




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database