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VASP RNA-Seq in HepG2



VASP      CRISPR in HepG2      Control:

General Information
RBPVASP
Cell_LineHepG2
MethodCRISPR
Exp_NameVASP-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1VASP-BGHcLV43-63
Rep2VASP-BGHcLV43-64
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR0.6
Rep2_qPCR4.7
Rep1_WB85.6
Rep2_WB78.7
Antibody Cat#3132S
Antibody Lot#lot # 5
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1594




Experiment Information (Status: Sequenced)
BGHcLV43-63BGHcLV43-64
idx14311432
TRCN#_or_BGC#BGC#0001205BGC#0001205
shRNA_or_gRNA_sequenceGGTTGTGGTAGATCTGGACGGGTTGTGGTAGATCTGGACG
PAMCGGCGG
NameVASP_90VASP_90
Sample_IDBGHcLV43-63BGHcLV43-64
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameVASPVASP
qPCR_result0.64.7
Ave_qPCR2.7
RT-qPCR_primer-Fcaacctccacaccctcaagt
RT-qPCR_primer-Rgcaatttgctgctttgtgaa
protein_conc35524450
WB_result85.678.7
Ave_WB82.1
WB_DONE_date6/21/24
MW46,50kd
IP
antibody_Cat#3132Slot # 5
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76387639




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
VASPProduct_ID: 3132S
Lot_ID: 5
Source: Cell Signaling Technology
Target Name: VASP-human		 VASP-HEPG2-CRISPR-3132S.png<br>Caption: Western blot following CRISPR against VASP in HepG2 whole cell lysate using VASP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against VASP. VASP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_3132S_5_VASP.png<br>Caption: IP-WB analysis of 3132S whole cell lysate using the VASP specific antibody, 3132S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the VASP-specific antibody, 3132S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 VASP-K562-CRISPR-3132S.png<br>Caption: Western blot following CRISPR against VASP in K562 whole cell lysate using VASP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against VASP. VASP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-63BGHcLV43-64
Sample_IDBGHcLV43-63BGHcLV43-64
Sample Name
Sample NameVASP-BGHcLV43-63VASP-BGHcLV43-64
RBPVASPVASP
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1327813279




Library-Prep Information
BGHcLV43-63BGHcLV43-64
Sample #5152
Sample NameVASP-BGHcLV43-63VASP-BGHcLV43-64
Sample_Name_Alias
Index Well PositionC12D12
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp297298
Peak_Molarity81.4065.10
libSampleQC_DNA_WellDNA_Library_set74/set2/C7.pngDNA_Library_set74/set2/D7.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/G12.pngRNA_Library_set74/set1/H12.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-63BGHcLV43-64
RBPVASPVASP
Batch_IDBGHcLV43BGHcLV43
WB_result85.60078.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID44104411




Sequencing Information
BGHcLV43-63BGHcLV43-64
Sample_IDBGHcLV43-63BGHcLV43-64
Sample NameVASP-BGHcLV43-63VASP-BGHcLV43-64
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads51,973,05249,629,913
total_aligned_reads49,109,62646,413,493
unique_aligned_reads46,181,26743,567,196
percent_uniqueAligned0.888560.87784
correlation_replicates0.9977950.997795
spikein_reads48,06813,520
percent_spikeins0.000920.00027
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32773278




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database