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SSU72 RNA-Seq in HepG2



SSU72      CRISPR in HepG2      Control:

General Information
RBPSSU72
Cell_LineHepG2
MethodCRISPR
Exp_NameSSU72-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1SSU72-BGHcLV43-53
Rep2SSU72-BGHcLV43-54
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR78.9
Rep2_qPCR77.7
Rep1_WB66.6
Rep2_WB66.3
Antibody Cat#12816S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1589




Experiment Information (Status: Sequenced)
BGHcLV43-53BGHcLV43-54
idx14211422
TRCN#_or_BGC#BGC#0001191BGC#0001191
shRNA_or_gRNA_sequenceGCGTCCGATCCTTTGGAACAGCGTCCGATCCTTTGGAACA
PAMGGGGGG
NameSSU72_91SSU72_91
Sample_IDBGHcLV43-53BGHcLV43-54
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameSSU72SSU72
qPCR_result78.977.7
Ave_qPCR78.3
RT-qPCR_primer-Fcaaccagaaccggagcat
RT-qPCR_primer-Ragcttcacgtgagtccctgt
protein_conc37824016
WB_result66.666.3
Ave_WB66.4
WB_DONE_date6/28/24
MW27kd
IP
antibody_Cat#12816Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76287629




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SSU72Product_ID: 12816S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: SSU72-human		 SSU72-HEPG2-CRISPR-12816S.png<br>Caption: Western blot following CRISPR against SSU72 in HepG2 whole cell lysate using SSU72 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SSU72. SSU72 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12816S_1_SSU72.png<br>Caption: IP-WB analysis of 12816S whole cell lysate using the SSU72 specific antibody, 12816S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SSU72-specific antibody, 12816S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SSU72-K562-CRISPR-12816S.png<br>Caption: Western blot following CRISPR against SSU72 in K562 whole cell lysate using SSU72 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SSU72. SSU72 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-53BGHcLV43-54
Sample_IDBGHcLV43-53BGHcLV43-54
Sample Name
Sample NameSSU72-BGHcLV43-53SSU72-BGHcLV43-54
RBPSSU72SSU72
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1326813269




Library-Prep Information
BGHcLV43-53BGHcLV43-54
Sample #4142
Sample NameSSU72-BGHcLV43-53SSU72-BGHcLV43-54
Sample_Name_Alias
Index Well PositionA11B11
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp296318
Peak_Molarity95.1098.30
libSampleQC_DNA_WellDNA_Library_set74/set1/A6.pngDNA_Library_set74/set1/B6.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/E11.pngRNA_Library_set74/set1/F11.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-53BGHcLV43-54
RBPSSU72SSU72
Batch_IDBGHcLV43BGHcLV43
WB_result66.60066.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID44004401




Sequencing Information
BGHcLV43-53BGHcLV43-54
Sample_IDBGHcLV43-53BGHcLV43-54
Sample NameSSU72-BGHcLV43-53SSU72-BGHcLV43-54
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads37,594,77942,915,220
total_aligned_reads32,360,47440,981,326
unique_aligned_reads30,373,44238,481,857
percent_uniqueAligned0.807920.89669
correlation_replicates0.9961240.996124
spikein_reads16,97611,428
percent_spikeins0.000450.00027
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32673268




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database