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EIF4H RNA-Seq in HepG2



EIF4H      CRISPR in HepG2      Control:

General Information
RBPEIF4H
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF4H-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF4H-BGHcLV43-21
Rep2EIF4H-BGHcLV43-22
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR10.6
Rep2_qPCR15.7
Rep1_WB94.8
Rep2_WB92.4
Antibody Cat#3469S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1576




Experiment Information (Status: Sequenced)
BGHcLV43-21BGHcLV43-22
idx13891390
TRCN#_or_BGC#BGC#0001139BGC#0001139
shRNA_or_gRNA_sequenceAATCCATGTTGGATCCACGGAATCCATGTTGGATCCACGG
PAMAGGAGG
NameEIF4H_74EIF4H_74
Sample_IDBGHcLV43-21BGHcLV43-22
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameEIF4HEIF4H
qPCR_result10.615.7
Ave_qPCR13.1
RT-qPCR_primer-Fatacggttcagggcgacata
RT-qPCR_primer-Rgggaatccacttcatcgaat
protein_conc33213973
WB_result94.892.4
Ave_WB93.6
WB_DONE_date6/13/24
MW25,27kd
IP
antibody_Cat#3469Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75947595




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF4HProduct_ID: 3469S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF4H-human		 EIF4H-HEPG2-CRISPR-3469S.png<br>Caption: Western blot following CRISPR against EIF4H in HepG2 whole cell lysate using EIF4H specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4H. EIF4H protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_3469S_1_EIF4H.png<br>Caption: IP-WB analysis of 3469S whole cell lysate using the EIF4H specific antibody, 3469S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF4H-specific antibody, 3469S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4H-K562-CRISPR-3469S.png<br>Caption: Western blot following CRISPR against EIF4H in K562 whole cell lysate using EIF4H specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4H. EIF4H protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-21BGHcLV43-22
Sample_IDBGHcLV43-21BGHcLV43-22
Sample Name
Sample NameEIF4H-BGHcLV43-21EIF4H-BGHcLV43-22
RBPEIF4HEIF4H
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1324213243




Library-Prep Information
BGHcLV43-21BGHcLV43-22
Sample #1516
Sample NameEIF4H-BGHcLV43-21EIF4H-BGHcLV43-22
Sample_Name_Alias
Index Well PositionG07H07
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp301287
Peak_Molarity79.1045.40
libSampleQC_DNA_WellDNA_Library_set74/set1/G2.pngDNA_Library_set74/set1/H2.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/C8.pngRNA_Library_set74/set1/D8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-21BGHcLV43-22
RBPEIF4HEIF4H
Batch_IDBGHcLV43BGHcLV43
WB_result94.80092.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43744375




Sequencing Information
BGHcLV43-21BGHcLV43-22
Sample_IDBGHcLV43-21BGHcLV43-22
Sample NameEIF4H-BGHcLV43-21EIF4H-BGHcLV43-22
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads34,084,78627,461,507
total_aligned_reads30,997,43525,156,604
unique_aligned_reads29,150,10823,629,998
percent_uniqueAligned0.855220.86048
correlation_replicates0.9973480.997348
spikein_reads19,38214,676
percent_spikeins0.000570.00053
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32413242




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database