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NAA10 RNA-Seq in HepG2



NAA10      CRISPR in HepG2      Control:

General Information
RBPNAA10
Cell_LineHepG2
MethodCRISPR
Exp_NameNAA10-BGHcLV43-HepG2
ENCODE_series_ID
Batch_IDBGHcLV43
Pool IDPool-240723
Local_Set_Nameset74
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1NAA10-BGHcLV43-31
Rep2NAA10-BGHcLV43-32
CN1NT-BGHcLV43-1
CN2NT-BGHcLV43-2
Rep1_qPCR87.5
Rep2_qPCR90.1
Rep1_WB58.7
Rep2_WB54.1
Antibody Cat#13357S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1580




Experiment Information (Status: Sequenced)
BGHcLV43-31BGHcLV43-32
idx13991400
TRCN#_or_BGC#BGC#0001158BGC#0001158
shRNA_or_gRNA_sequenceACATTGCTGAGGACGAGAATACATTGCTGAGGACGAGAAT
PAMGGGGGG
NameNAA10_87NAA10_87
Sample_IDBGHcLV43-31BGHcLV43-32
transduction_Date6/6/246/6/24
daysD6D6
RBP_nameNAA10NAA10
qPCR_result87.590.1
Ave_qPCR88.8
RT-qPCR_primer-Fttctaccatggcctttcctg
RT-qPCR_primer-Rccaggacataccccacaatc
protein_conc36273898
WB_result58.754.1
Ave_WB56.4
WB_DONE_date6/17/24
MW28kd
IP
antibody_Cat#13357Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76047605




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NAA10Product_ID: 13357S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NAA10-human		 NAA10-HEPG2-CRISPR-13357S.png<br>Caption: Western blot following CRISPR against NAA10 in HepG2 whole cell lysate using NAA10 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NAA10. NAA10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13357S_1_NAA10.png<br>Caption: IP-WB analysis of 13357S whole cell lysate using the NAA10 specific antibody, 13357S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NAA10-specific antibody, 13357S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NAA10-K562-CRISPR-13357S.png<br>Caption: Western blot following CRISPR against NAA10 in K562 whole cell lysate using NAA10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NAA10. NAA10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV43-31BGHcLV43-32
Sample_IDBGHcLV43-31BGHcLV43-32
Sample Name
Sample NameNAA10-BGHcLV43-31NAA10-BGHcLV43-32
RBPNAA10NAA10
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1325013251




Library-Prep Information
BGHcLV43-31BGHcLV43-32
Sample #2324
Sample NameNAA10-BGHcLV43-31NAA10-BGHcLV43-32
Sample_Name_Alias
Index Well PositionG08H08
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-07-222024-07-22
Lib_IDLib-240722Lib-240722
Tecan_Location
Tecan
Tecan_date
Size_bp289286
Peak_Molarity82.0063.40
libSampleQC_DNA_WellDNA_Library_set74/set1/G3.pngDNA_Library_set74/set1/H3.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set74/set1/C9.pngRNA_Library_set74/set1/D9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-232024-07-23
Sample_IDBGHcLV43-31BGHcLV43-32
RBPNAA10NAA10
Batch_IDBGHcLV43BGHcLV43
WB_result58.70054.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43824383




Sequencing Information
BGHcLV43-31BGHcLV43-32
Sample_IDBGHcLV43-31BGHcLV43-32
Sample NameNAA10-BGHcLV43-31NAA10-BGHcLV43-32
Pool IDPool-240723Pool-240723
LocalServer_folderset74set74
total_reads43,449,23231,297,960
total_aligned_reads39,547,29928,357,678
unique_aligned_reads37,209,34126,683,246
percent_uniqueAligned0.856390.85256
correlation_replicates0.9981970.998197
spikein_reads26,17015,818
percent_spikeins0.000600.00051
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32493250




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database