SEC23IP

General Information

Gene Name       : SEC23IP
Gene ID             : ENSG00000107651.12
Essential Gene : No

Functions

Localization

Domains (AlphaFold: SEC23IP)


Antibody Validation

 View validation images for all SEC23IP antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878SEC23IPENSG00000107651.1242.9546.5644.76ENCSR000AEF
HepG2SEC23IPENSG00000107651.1234.9946.4440.72ENCSR985KAT
K562SEC23IPENSG00000107651.1221.1025.8223.46ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq BGKcLV33-29,BGKcLV33-30SEC23IPK562Released   CRISPRKD% = no band on the position,
knockdown followed by RNA-seq SEC23IP-BGHcLV31-HepG2ENCSR745KNESEC23IPHepG2ReleasedENCSR452TFV   CRISPRKD% = 87.1,87.6






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
SEC23IPENCAB911OUOA300-592A1Released, ENCODE4BGHcLV31-31, Released, ENCODE4
SEC23IPENCAB820MEZA300-594A1Released, ENCODE4BGHcLV31-31, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
SEC23IPK562CRISPRBGKcLV33-29,BGKcLV33-30BGKcLV33-1,BGKcLV33-2no band on the position,ReleasedENCODE4
SEC23IPK562CRISPRBGKcLV33-35,BGKcLV33-36BGKcLV33-1,BGKcLV33-2no band,NotSatisfiedENCODE4
SEC23IPHepG2CRISPRENCSR745KNEENCSR452TFVSEC23IP-BGHcLV31-31,SEC23IP-BGHcLV31-32NT-BGHcLV31-1,NT-BGHcLV31-287.1,87.6ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SEC23IPProduct_ID: A300-592A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SEC23IP-human		 SEC23IP-HEPG2-CRISPR-A300-592A.png<br>Caption: Western blot following CRISPR against SEC23IP in HepG2 whole cell lysate using SEC23IP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEC23IP. SEC23IP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A300-592A_1_SEC23IP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SEC23IP specific antibody, A300-592A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SEC23IP-specific antibody, A300-592A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
SEC23IPProduct_ID: A300-594A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SEC23IP-human		 SEC23IP-HEPG2-CRISPR-A300-594A.png<br>Caption: Western blot following CRISPR against SEC23IP in HepG2 whole cell lysate using SEC23IP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEC23IP. SEC23IP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A300-594A_1_SEC23IP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SEC23IP specific antibody, A300-594A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SEC23IP-specific antibody, A300-594A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for SEC23IP is not avaliable on ENCORE database.



Proteins bound to RNA
  • SEC23IP RNA is bound by 19 proteins.  







eCLIP assay for SEC23IP is not avaliable on ENCORE database.





19 proteins are bound to SEC23IP RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr10121693197121693278+3'-UTR,CDS,intron-Proximal3.086453.01994K562BUD13
chr10121701092121701144+3'-UTR,intron-Distal4.829405.30083K562DGCR8
chr10121700556121700628+3'-UTR3.153424.10408K562KHSRP
chr10121658226121658324+CDS,intron-Distal3.469153.77614HepG2G3BP1
chr10121658084121658151+CDS,intron-Distal4.206173.98311K562IGF2BP1
chr10121657979121658084+CDS,intron-Distal4.406883.25872K562IGF2BP1
chr10121693186121693232+CDS,intron-Proximal3.616373.95921HepG2U2AF2
chr10121691700121691788+CDS3.508154.04629HepG2G3BP1
chr10121652404121652458+CDS,intron-Proximal4.053523.18462K562IGF2BP2
chr10121674236121674283+CDS,intron-Proximal3.849944.96634HepG2PPIG
chr10121685547121685612+CDS,intron-Proximal3.006743.67092HepG2G3BP1
chr10121691763121691809+CDS3.190615.40132HepG2BCLAF1
chr10121685546121685567+CDS,intron-Proximal5.124077.04013HepG2BCLAF1
chr10121677969121678022+CDS3.130114.23325HepG2BCLAF1
chr10121692651121692748+CDS,intron-Proximal5.055275.68502HepG2PRPF8
chr10121671551121671620+CDS,intron-Proximal3.485004.47362HepG2G3BP1
chr10121691756121691820+CDS3.594593.95301K562ZNF622
chr10121692521121692562+CDS4.305633.79000HepG2U2AF2
chr10121691663121691708+CDS3.083555.35431HepG2BCLAF1
chr10121658287121658404+CDS,intron-Distal3.776755.36986K562RBM15
chr10121691763121691830+CDS3.500523.87318HepG2TRA2A
chr10121691743121691865+CDS,intron-Proximal3.518396.43326K562UCHL5
chr10121691761121691858+CDS,intron-Proximal4.492045.93840HepG2PRPF8
chr10121658310121658458+CDS,intron-Distal5.3363310.37403K562IGF2BP2
chr10121680419121680497+CDS,intron-Proximal3.201978.30744HepG2BCLAF1
chr10121680416121680481+CDS4.174033.59941K562UCHL5
chr10121685567121685613+CDS4.165306.65960HepG2BCLAF1
chr10121691890121692007+intron-Distal,intron-Proximal3.988773.24603K562KHSRP
chr10121692300121692415+intron-Distal4.425404.47532K562U2AF2
chr10121695457121695587+intron-Distal3.830573.71397K562KHSRP
chr10121692262121692381+intron-Distal4.626438.21761HepG2PTBP1
chr10121692292121692414+intron-Distal4.1775713.35401HepG2U2AF2
chr10121697287121697425+intron-Distal4.143944.07352K562KHSRP
chr10121685960121686030+intron-Distal3.416833.32861HepG2U2AF2
chr10121659843121659882+intron-Distal3.302964.25372HepG2HNRNPC
chr10121664176121664236+intron-Distal3.088523.40825HepG2CSTF2
chr10121659469121659575+intron-Distal5.563679.00303HepG2PCBP2
chr10121659833121659843+intron-Distal4.201254.95101HepG2HNRNPC
chr10121692273121692361+intron-Distal3.428286.26469K562PTBP1
chr10121693386121693436+intron-Distal,intron-Proximal4.651574.64001HepG2U2AF2
chr10121695052121695117+intron-Distal3.204833.70624HepG2HNRNPK
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for SEC23IP is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for SEC23IP is not available on ENCORE database.

  • 81 RBPs can affect the levels of SEC23IP.  








The Knockdown/knockout RNA-Seq assay for SEC23IP is not available on ENCORE database.





81 RBPs can affect the gene expression of SEC23IP :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
SEC23IPENSG00000107651.81507.694001941.97770-0.365202.588101.67680HepG2ABCF1
SEC23IPENSG00000107651.81173.497401499.12770-0.353302.657601.67910HepG2ADAR
SEC23IPENSG00000107651.81258.241501756.50270-0.481303.095502.10360HepG2BCCIP
SEC23IPENSG00000107651.82618.963401929.010000.441006.070804.98370HepG2CALR
SEC23IPENSG00000107651.82239.664201550.181900.530705.523504.45280HepG2CIRBP
SEC23IPENSG00000107651.82320.867001611.490200.5262014.1567012.89560HepG2CKAP4
SEC23IPENSG00000107651.81208.379201998.38970-0.725809.728908.51520HepG2CPSF6
SEC23IPENSG00000107651.81817.954502275.63170-0.324006.307205.25450HepG2DAZAP1
SEC23IPENSG00000107651.8965.463001308.51840-0.438602.959001.51790HepG2DDX28
SEC23IPENSG00000107651.81293.617301726.93240-0.416802.399901.67400HepG2DDX55
SEC23IPENSG00000107651.81334.641001542.13510-0.208501.948401.36490HepG2DKC1
SEC23IPENSG00000107651.81097.737701509.56750-0.459606.607505.55650HepG2DNAJC21
SEC23IPENSG00000107651.81486.718301133.342500.391403.139801.95990HepG2EFTUD2
SEC23IPENSG00000107651.8897.837601723.85300-0.9411011.9497010.64700HepG2EIF4A3
SEC23IPENSG00000107651.81748.285002150.46730-0.298702.402801.64150HepG2EIF4G2
SEC23IPENSG00000107651.81548.601602591.67540-0.7429021.7595020.34920HepG2ETF1
SEC23IPENSG00000107651.81410.57010982.016500.522405.709104.59160HepG2FUBP3
SEC23IPENSG00000107651.81365.211901876.41970-0.458902.763901.64940HepG2FUS
SEC23IPENSG00000107651.8637.23460982.60930-0.624806.031104.70560HepG2GEMIN5
SEC23IPENSG00000107651.81112.381301429.14650-0.361502.393301.45030HepG2GNB2L1
SEC23IPENSG00000107651.81689.070002265.34160-0.423503.336002.63720HepG2GPKOW
SEC23IPENSG00000107651.8687.432301695.18650-1.3022029.3390027.37490HepG2HNRNPC
SEC23IPENSG00000107651.81450.020901834.58560-0.339404.694703.79050HepG2HNRNPF
SEC23IPENSG00000107651.81294.699702072.99300-0.6791021.7145020.00360HepG2HNRNPL
SEC23IPENSG00000107651.81295.812502161.85620-0.738409.960908.84990HepG2HNRNPLL
SEC23IPENSG00000107651.81654.907201353.264700.290202.665001.72960HepG2HNRNPM
SEC23IPENSG00000107651.81047.152601683.98000-0.6854016.4554015.07350HepG2HNRNPU
SEC23IPENSG00000107651.81390.608501806.54490-0.377504.255503.37430HepG2IGF2BP1
SEC23IPENSG00000107651.81140.084501473.44130-0.370006.042504.56150HepG2KIF1C
SEC23IPENSG00000107651.82110.160202780.55090-0.398002.573301.77400HepG2LARP4
SEC23IPENSG00000107651.81701.761902256.50000-0.407105.831804.72860HepG2NOL12
SEC23IPENSG00000107651.82121.820101501.356900.4989010.007408.96830HepG2PCBP1
SEC23IPENSG00000107651.8667.11160912.62720-0.452104.386002.83580HepG2PKM
SEC23IPENSG00000107651.81164.422501501.97810-0.367204.423403.51120HepG2PPIL4
SEC23IPENSG00000107651.81907.277501588.041900.264203.052202.24480HepG2PRPF4
SEC23IPENSG00000107651.81908.833101527.671100.321304.444303.78640HepG2PTBP1
SEC23IPENSG00000107651.81532.427602153.96570-0.491206.294205.44390HepG2PUF60
SEC23IPENSG00000107651.82019.433502286.45820-0.179202.602402.02330HepG2PUM1
SEC23IPENSG00000107651.81880.845502284.83250-0.280702.977902.28200HepG2PUM2
SEC23IPENSG00000107651.82210.710001641.814800.429108.776707.78230HepG2PUS1
SEC23IPENSG00000107651.81852.069002166.14300-0.226002.235801.75660HepG2RBM22
SEC23IPENSG00000107651.81348.278301688.17970-0.324402.215101.33670HepG2RBM27
SEC23IPENSG00000107651.81260.416201976.27100-0.648909.142408.01560HepG2RPLP0
SEC23IPENSG00000107651.81312.227101499.66690-0.192601.915101.36210HepG2SAFB2
SEC23IPENSG00000107651.81942.093701590.531000.288003.570302.91110HepG2SF3B4
SEC23IPENSG00000107651.82959.543602446.546900.274502.953502.44130HepG2SRFBP1
SEC23IPENSG00000107651.82151.029401660.158500.373606.060405.42730HepG2SRSF1
SEC23IPENSG00000107651.81264.880002302.43210-0.8642010.061608.05190HepG2SRSF3
SEC23IPENSG00000107651.81764.500902126.64170-0.269303.601902.90470HepG2SRSF5
SEC23IPENSG00000107651.81340.433901802.41350-0.427206.925005.90450HepG2SRSF7
SEC23IPENSG00000107651.81204.868901719.25220-0.512903.613802.85650HepG2SUPV3L1
SEC23IPENSG00000107651.81398.279901636.35390-0.226801.792901.37250HepG2TIAL1
SEC23IPENSG00000107651.8834.892201650.92520-0.9836030.5397028.91220HepG2TRIM56
SEC23IPENSG00000107651.82308.731301677.746800.460504.830804.01360HepG2U2AF1
SEC23IPENSG00000107651.8651.511801015.69560-0.640605.714304.61380HepG2UCHL5
SEC23IPENSG00000107651.81738.668402058.65950-0.243703.056702.33070HepG2UPF1
SEC23IPENSG00000107651.81070.855001574.61850-0.556203.047401.99340HepG2UPF2
SEC23IPENSG00000107651.81657.067601987.55610-0.262403.296502.65300HepG2UTP3
SEC23IPENSG00000107651.81352.774001885.11680-0.478702.982401.87890HepG2XRCC6
SEC23IPENSG00000107651.81214.540501692.41170-0.478703.559602.74550HepG2XRN2
SEC23IPENSG00000107651.8969.84240759.168800.353202.557301.42760K562AARS
SEC23IPENSG00000107651.81651.102701142.027000.531704.480403.64180K562AKAP8L
SEC23IPENSG00000107651.81553.485401198.293700.374404.804003.56570K562ATP5C1
SEC23IPENSG00000107651.8765.65460991.79050-0.373303.137502.30150K562CPSF6
SEC23IPENSG00000107651.8747.28480963.33680-0.366402.711301.89870K562EIF4A3
SEC23IPENSG00000107651.8872.820801099.59420-0.333202.415201.62330K562FIP1L1
SEC23IPENSG00000107651.8471.93380746.27060-0.661105.484803.46250K562GEMIN5
SEC23IPENSG00000107651.8990.843401397.28840-0.495905.431704.36820K562GRSF1
SEC23IPENSG00000107651.8594.61150830.90570-0.482704.053703.34810K562HNRNPC
SEC23IPENSG00000107651.81111.938901490.81690-0.423003.824302.89270K562HSPD1
SEC23IPENSG00000107651.8917.736701155.12360-0.331902.642601.82100K562MAGOH
SEC23IPENSG00000107651.8763.186001152.84780-0.595106.879105.33900K562MATR3
SEC23IPENSG00000107651.81703.619201357.660100.327403.031002.07050K562MBNL1
SEC23IPENSG00000107651.81358.11450951.626700.513003.130302.18290K562NAA15
SEC23IPENSG00000107651.8623.86860791.09710-0.342602.524301.76190K562POLR2G
SEC23IPENSG00000107651.81003.646801248.25640-0.314702.173001.44990K562PPIG
SEC23IPENSG00000107651.81003.81490724.348000.470603.711602.84250K562PRPF8
SEC23IPENSG00000107651.81656.131801132.454400.548306.068005.30200K562PUS1
SEC23IPENSG00000107651.81399.467901103.508100.342703.343502.70130K562RBM15
SEC23IPENSG00000107651.81095.08660737.831300.569604.573603.52050K562RPS19
SEC23IPENSG00000107651.81591.41520744.820701.095205.621603.71070K562SF1
SEC23IPENSG00000107651.81408.659801215.112500.213102.134701.30320K562SRPK2
SEC23IPENSG00000107651.8671.05370855.26590-0.349902.443001.46330K562SRSF4
SEC23IPENSG00000107651.8990.32850793.474900.319602.350901.59390K562SYNCRIP
SEC23IPENSG00000107651.8853.231601113.50190-0.384102.731602.02800K562TARDBP
SEC23IPENSG00000107651.8834.070201074.07010-0.364803.074702.20370K562TIA1
SEC23IPENSG00000107651.81258.08440958.948400.391602.540401.49850K562U2AF1
SEC23IPENSG00000107651.81782.953601274.522500.484209.092307.88320K562UPF1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for SEC23IP is not available on ENCORE database.





13 RBPs can affect the splicing of SEC23IP :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SESEC23IPENSG00000107651.8chr10+121689840121689943121689118121689274121691644121691830-0.192000.003080.05094excludedK562SF3B4
SESEC23IPENSG00000107651.8chr10+121689118121689274121685547121685739121689840121689943-0.145000.000010.02146excludedHepG2IGF2BP1
SESEC23IPENSG00000107651.8chr10+121689118121689274121685547121685739121689840121689943-0.275000.000000.00000excludedHepG2U2AF1
SESEC23IPENSG00000107651.8chr10+121689118121689274121685547121685739121689840121689943-0.251000.000000.00001excludedHepG2U2AF2
SESEC23IPENSG00000107651.8chr10+121689840121689943121689118121689274121691644121691830-0.147000.000330.00482excludedK562AQR
SESEC23IPENSG00000107651.8chr10+121692516121692679121691644121691830121693197121693299-0.080000.007640.08564excludedK562PUS1
SESEC23IPENSG00000107651.8chr10+121689118121689274121685547121685739121689840121689943-0.131000.000210.03809excludedHepG2HNRNPA1
SESEC23IPENSG00000107651.8chr10+121657938121658471121652222121652457121662310121662521-0.118000.005600.09602excludedHepG2SRSF1
SESEC23IPENSG00000107651.8chr10+121689840121689943121689118121689274121691644121691830-0.068000.001540.04277excludedHepG2SF3A3
SESEC23IPENSG00000107651.8chr10+121692516121692679121691644121691830121693197121693299-0.070000.000480.03982excludedK562HNRNPUL1
SESEC23IPENSG00000107651.8chr10+121689118121689274121685547121685739121689840121689943-0.100000.004950.07202excludedK562U2AF2
SESEC23IPENSG00000107651.8chr10+121689840121689943121689118121689274121691644121691830-0.177000.000080.00351excludedK562RBM15
SESEC23IPENSG00000107651.8chr10+121689118121689274121685547121685739121689840121689943-0.242000.000000.00000excludedHepG2HNRNPU
SESEC23IPENSG00000107651.8chr10+121657938121658471121652222121652457121662310121662521-0.144000.002210.03694excludedK562HNRNPC
A3SSSEC23IPENSG00000107651.8chr10+1216931731216932991216931971216932991216925161216926790.084000.005480.03135longerK562AQR
A3SSSEC23IPENSG00000107651.8chr10+1216803661216804961216804001216804961216789551216791080.129000.000010.00013longerK562AQR
A3SSSEC23IPENSG00000107651.8chr10+1216742191216744621216742411216744621216715551216716760.112000.001060.00735longerK562AQR
A5SSSEC23IPENSG00000107651.8chr10+1216804001216805871216804001216804961216855471216857390.138000.000000.00000longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for SEC23IP is not avaliable on ENCORE database.