SEC63      CRISPR in K562      Control: NT-BGKcLV33

General Information
RBPSEC63
Cell_LineK562
MethodCRISPR
Exp_NameSEC63-BGKcLV33-K562
ENCODE_series_IDENCSR058HZP
Batch_IDBGKcLV33
Pool IDPool-220418
Local_Set_Nameset53
ENCODE_KD_Exp_IDENCSR082BCP
ENCODE_CN_Exp_IDENCSR271ZAB
Rep1SEC63-BGKcLV33-41
Rep2SEC63-BGKcLV33-42
CN1NT-BGKcLV33-1
CN2NT-BGKcLV33-2
Rep1_qPCR
Rep2_qPCR
Rep1_WB0.0
Rep2_WB0.0
Antibody Cat#A304-212A
Antibody Lot#LOT #1
Antibody DCC ID
StatusSubmitted
ProjectENCODE4
ID904




Experiment Information (Status: NotSatisfied)
BGKcLV33-35BGKcLV33-35BGKcLV33-36BGKcLV33-36
idx1137122211381223
TRCN#_or_BGC#BGC#0000853BGC#0000848BGC#0000853BGC#0000848
shRNA_or_gRNA_sequenceGATCACGATGAGCCCCACGAGCCGAGAGAAAACCTAACGGGATCACGATGAGCCCCACGAGCCGAGAGAAAACCTAACGG
PAMAGGTGGAGGTGG
NameSEC63_94SEC23IP_96SEC63_94SEC23IP_96
Sample_IDBGKcLV33-35BGKcLV33-35BGKcLV33-36BGKcLV33-36
transduction_Date12/7/212/22/2212/7/212/22/22
daysD6D6D6D6
RBP_nameSEC63SEC23IPSEC63SEC23IP
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc37420654821800
WB_result74.7no band72.1
Ave_WB73.4
WB_DONE_date1/24/223/14/22, 3/21/22,3/2Licor, jess
MW88kd111kd
IP
antibody_Cat#A305-084AA300-592ALOT #1LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM014.400
Rep2_TPM017.300
Action
Library_start_date
repeat_library
Note
ID5277536452785365




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV33-35BGKcLV33-35BGKcLV33-36BGKcLV33-36
Sample_IDBGKcLV33-35BGKcLV33-35BGKcLV33-36BGKcLV33-36
Sample Name
Sample Name
RBPSEC63SEC23IPSEC63SEC23IP
Cell_LineK562K562K562K562
Exp UID
StatusNotSatisfiedNotSatisfiedNotSatisfiedNotSatisfied
Status_date2022-03-302022-03-302022-03-302022-03-30
ProjectENCODE4ENCODE4ENCODE4ENCODE4
Note
ID10721107771072210778




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database